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The map label for this gene is yxeI [H]

Identifier: 108758199

GI number: 108758199

Start: 6727703

End: 6728773

Strand: Reverse

Name: yxeI [H]

Synonym: MXAN_5407

Alternate gene names: 108758199

Gene position: 6728773-6727703 (Counterclockwise)

Preceding gene: 108757414

Following gene: 108757083

Centisome position: 73.62

GC content: 65.17

Gene sequence:

>1071_bases
ATGTGCACTGATTTTCTGGTCGCGACCGAGGACTCGAGCGCTGTCGTCGTTGGCCGCAGCATGGAGTTCGGCATCGACCT
CGGCTCCAAGTTCCGGTTCCACGCCAAGGGGCATACGGTCCAGTCTCCGGGTTCCCTCACGCACCTGCATGGCCTCAAGT
GGACGACGAAGTTCGACTACGTCGGCCTCACCGTCACGCTCTATGGGGTCACCGTCATCGGCGATGCGATGAACTCGGAA
GGCCTCTCCGCCGGAGCGCTGTGGATGCCCACGTCCACGTATCCCCAGGCCGCCAAGGACCCGTCCCGGGGACTGGCGCC
CGAGCTCTTCGTGGAGTGGGCCGTGGGTAACTTCGCCACCGTGGACGCGGTGAAGGAGGCCCTGGCGAACGGAGACGCCG
AGCTGTGGCACAGCGACTGGATGTCGAGCAAGATTCCGCTCCACTTCGCCCTCCACGACGCGAAGGGCAACAGCATGGTC
GTCGAGTTCACCGACCGGCAGATGTTCCTGTACGCGTCGACGGGTCATGACAACTCGCCGCTGAACATGCACAACCCCGT
TGCTGTCCTGACCAACCACCCGACCTTCCCGGACCACCTGGAGAACCTCAAGAACTACGCGAGGCTGTCGTCCCGTGGCG
CCACGCCCGTGAAGCTGGGAACCGGCACATTCGCACCTCACGGCTGCGGCAGCGGCCTCTTCGGGATTCCCGGCGACTCC
ACGCCTCCCTCCCGGTTCGTCCGGGCCGTCTACCTCCGTGAGTTCTCCGAGAAGCCCAAGAACGCGGCCGAGGCCCGCTC
CCTGGCGCTGCACCTGCTCAACACCGTCGACATCCCCTACGGCACCAGCCGCGACTTGAACGAGGCCACGGGCAAGGACT
CTCTCGACTACACCCAGTGGGTCGTCGTGAAGGACCTCACGAACCGCACGTTCGGCGTCCGGACCTACGACAGCCTGGGG
CTGCAGACGCTGGACCTGCGCACGGTCGATTTCAAGAAGGCGGATGGCAAGACGCTCGCCGTCTCCCCGGTGGATTCGCA
GCTCGACGCCAACAAGCAGCTGGCGAGCTGA

Upstream 100 bases:

>100_bases
CCCGCGAGGCTCAGGTGGCACGCGTCCTGCTCTGGTGACGCACTGCCCCCCCAACACCAGACCTTCGCATGGCGCGAAGG
CATCAACGAGGACGTTTCAC

Downstream 100 bases:

>100_bases
GTCACTGACTCCCCCTCCGTGGCGCGGGGCCGGGCACCTGGCTGCAAACGGGTACCCGGCCCCGCGTCGTTGGCTTCACT
CGGAACGCCGCGCGCCTGGA

Product: choloylglycine hydrolase family protein

Products: 3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate; glycine

Alternate protein names: NA

Number of amino acids: Translated: 356; Mature: 356

Protein sequence:

>356_residues
MCTDFLVATEDSSAVVVGRSMEFGIDLGSKFRFHAKGHTVQSPGSLTHLHGLKWTTKFDYVGLTVTLYGVTVIGDAMNSE
GLSAGALWMPTSTYPQAAKDPSRGLAPELFVEWAVGNFATVDAVKEALANGDAELWHSDWMSSKIPLHFALHDAKGNSMV
VEFTDRQMFLYASTGHDNSPLNMHNPVAVLTNHPTFPDHLENLKNYARLSSRGATPVKLGTGTFAPHGCGSGLFGIPGDS
TPPSRFVRAVYLREFSEKPKNAAEARSLALHLLNTVDIPYGTSRDLNEATGKDSLDYTQWVVVKDLTNRTFGVRTYDSLG
LQTLDLRTVDFKKADGKTLAVSPVDSQLDANKQLAS

Sequences:

>Translated_356_residues
MCTDFLVATEDSSAVVVGRSMEFGIDLGSKFRFHAKGHTVQSPGSLTHLHGLKWTTKFDYVGLTVTLYGVTVIGDAMNSE
GLSAGALWMPTSTYPQAAKDPSRGLAPELFVEWAVGNFATVDAVKEALANGDAELWHSDWMSSKIPLHFALHDAKGNSMV
VEFTDRQMFLYASTGHDNSPLNMHNPVAVLTNHPTFPDHLENLKNYARLSSRGATPVKLGTGTFAPHGCGSGLFGIPGDS
TPPSRFVRAVYLREFSEKPKNAAEARSLALHLLNTVDIPYGTSRDLNEATGKDSLDYTQWVVVKDLTNRTFGVRTYDSLG
LQTLDLRTVDFKKADGKTLAVSPVDSQLDANKQLAS
>Mature_356_residues
MCTDFLVATEDSSAVVVGRSMEFGIDLGSKFRFHAKGHTVQSPGSLTHLHGLKWTTKFDYVGLTVTLYGVTVIGDAMNSE
GLSAGALWMPTSTYPQAAKDPSRGLAPELFVEWAVGNFATVDAVKEALANGDAELWHSDWMSSKIPLHFALHDAKGNSMV
VEFTDRQMFLYASTGHDNSPLNMHNPVAVLTNHPTFPDHLENLKNYARLSSRGATPVKLGTGTFAPHGCGSGLFGIPGDS
TPPSRFVRAVYLREFSEKPKNAAEARSLALHLLNTVDIPYGTSRDLNEATGKDSLDYTQWVVVKDLTNRTFGVRTYDSLG
LQTLDLRTVDFKKADGKTLAVSPVDSQLDANKQLAS

Specific function: Unknown

COG id: COG3049

COG function: function code M; Penicillin V acylase and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase C59 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003199 [H]

Pfam domain/function: PF02275 CBAH [H]

EC number: 3.5.1.24

Molecular weight: Translated: 38680; Mature: 38680

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCTDFLVATEDSSAVVVGRSMEFGIDLGSKFRFHAKGHTVQSPGSLTHLHGLKWTTKFDY
CCCCEEEEECCCCEEEEECCEEECCCCCCEEEEEECCCEECCCCCEEEECCCEEEEEECE
VGLTVTLYGVTVIGDAMNSEGLSAGALWMPTSTYPQAAKDPSRGLAPELFVEWAVGNFAT
EEEEEEEEEEEEEECCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCHHHEEEECCCCCHH
VDAVKEALANGDAELWHSDWMSSKIPLHFALHDAKGNSMVVEFTDRQMFLYASTGHDNSP
HHHHHHHHHCCCHHHHHHHCCCCCCCEEEEEEECCCCEEEEEEECCEEEEEEECCCCCCC
LNMHNPVAVLTNHPTFPDHLENLKNYARLSSRGATPVKLGTGTFAPHGCGSGLFGIPGDS
CCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCCCC
TPPSRFVRAVYLREFSEKPKNAAEARSLALHLLNTVDIPYGTSRDLNEATGKDSLDYTQW
CCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCCCCCEEE
VVVKDLTNRTFGVRTYDSLGLQTLDLRTVDFKKADGKTLAVSPVDSQLDANKQLAS
EEEEECCCCEECEEEHHHCCCEEEEEEEEEEECCCCCEEEECCCCCHHCCCHHCCC
>Mature Secondary Structure
MCTDFLVATEDSSAVVVGRSMEFGIDLGSKFRFHAKGHTVQSPGSLTHLHGLKWTTKFDY
CCCCEEEEECCCCEEEEECCEEECCCCCCEEEEEECCCEECCCCCEEEECCCEEEEEECE
VGLTVTLYGVTVIGDAMNSEGLSAGALWMPTSTYPQAAKDPSRGLAPELFVEWAVGNFAT
EEEEEEEEEEEEEECCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCHHHEEEECCCCCHH
VDAVKEALANGDAELWHSDWMSSKIPLHFALHDAKGNSMVVEFTDRQMFLYASTGHDNSP
HHHHHHHHHCCCHHHHHHHCCCCCCCEEEEEEECCCCEEEEEEECCEEEEEEECCCCCCC
LNMHNPVAVLTNHPTFPDHLENLKNYARLSSRGATPVKLGTGTFAPHGCGSGLFGIPGDS
CCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCCCC
TPPSRFVRAVYLREFSEKPKNAAEARSLALHLLNTVDIPYGTSRDLNEATGKDSLDYTQW
CCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCCCCCEEE
VVVKDLTNRTFGVRTYDSLGLQTLDLRTVDFKKADGKTLAVSPVDSQLDANKQLAS
EEEEECCCCEECEEEHHHCCCEEEEEEEEEEECCCCCEEEECCCCCHHCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3alpha,7alpha,12alpha-trihydroxy-5beta-cholan-24-oylglycine; H2O

Specific reaction: 3alpha,7alpha,12alpha-trihydroxy-5beta-cholan-24-oylglycine + H2O = 3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate + glycine

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8867804; 9384377 [H]