Click here to switch to the map view.

The map label for this gene is nuoB

Identifier: 108758077

GI number: 108758077

Start: 3184275

End: 3184838

Strand: Reverse

Name: nuoB

Synonym: MXAN_2733

Alternate gene names: 108758077

Gene position: 3184838-3184275 (Counterclockwise)

Preceding gene: 108762570

Following gene: 108759239

Centisome position: 34.85

GC content: 66.31

Gene sequence:

>564_bases
ATGGCTGACGCTGACCTCGCAAACATCGTGACGACCCGCCGTGACGAGTCCATGGGCTGGCTCCAGTCCATCGTCTCCAA
GGGCCTCGGTTGGGCGCGCAAGTACTCGCTCTTCACCTATCCGTACGCCACGGCCTGCTGCGGCATGGAGTACATGTCCG
TGGCCGCCAGCCGGCACGACATCTCCCGCTTCGGCGCGGAGTTCCCCCGCTTCTCGCCGCGCCAGGCGGACCTGCTGATG
GTGGTGGGCACCATCAACCTGAAGCAGGCCCCCATCCTCAAGCGCGTCTATGAGCAGATGGCCGAGCCCAAGTGGGTCGT
GGCCTTCGGCGTGTGCGCGTCCTCGGGCGGCTTCTACGACAACTACGCGGTGCTCCAGGGCATCGACCGCATCATCCCGG
TGGACGTCTACATCCCCGGCTGCCCGCCGCGCCCGGAGCAGGTGCTCGACGGCCTGATGCTCCTGCAGGACAAGATTGGC
AACCAGGTCCACCGCATCGCGGAGCGCGAGGAGGCCAACCCCACGGCCGCTCGGCACAACCTGCTGCTGTCCATGAACAA
GTAA

Upstream 100 bases:

>100_bases
GTTCGGGTACGTGGAGATGCTGGTTTTCGCCGTGACGCTGGTGGTCGGCCTTATCTATATCTGGAAGAAGGGCGCCCTGG
ACTGGGAGAGCTGAGACATC

Downstream 100 bases:

>100_bases
CGCGCCCCACGCGCGGTACCGCCAGGGCCCCGCCCGCTTCCCGCCTCAGTCGGTGGAAGCGCCGGGGCCTTGGTATTTCT
GGCGCCACTACCAGCGATGG

Product: NADH dehydrogenase subunit B

Products: NA

Alternate protein names: NADH dehydrogenase I subunit B; NDH-1 subunit B

Number of amino acids: Translated: 187; Mature: 186

Protein sequence:

>187_residues
MADADLANIVTTRRDESMGWLQSIVSKGLGWARKYSLFTYPYATACCGMEYMSVAASRHDISRFGAEFPRFSPRQADLLM
VVGTINLKQAPILKRVYEQMAEPKWVVAFGVCASSGGFYDNYAVLQGIDRIIPVDVYIPGCPPRPEQVLDGLMLLQDKIG
NQVHRIAEREEANPTAARHNLLLSMNK

Sequences:

>Translated_187_residues
MADADLANIVTTRRDESMGWLQSIVSKGLGWARKYSLFTYPYATACCGMEYMSVAASRHDISRFGAEFPRFSPRQADLLM
VVGTINLKQAPILKRVYEQMAEPKWVVAFGVCASSGGFYDNYAVLQGIDRIIPVDVYIPGCPPRPEQVLDGLMLLQDKIG
NQVHRIAEREEANPTAARHNLLLSMNK
>Mature_186_residues
ADADLANIVTTRRDESMGWLQSIVSKGLGWARKYSLFTYPYATACCGMEYMSVAASRHDISRFGAEFPRFSPRQADLLMV
VGTINLKQAPILKRVYEQMAEPKWVVAFGVCASSGGFYDNYAVLQGIDRIIPVDVYIPGCPPRPEQVLDGLMLLQDKIGN
QVHRIAEREEANPTAARHNLLLSMNK

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG0377

COG function: function code C; NADH:ubiquinone oxidoreductase 20 kD subunit and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family

Homologues:

Organism=Homo sapiens, GI187281616, Length=135, Percent_Identity=50.3703703703704, Blast_Score=149, Evalue=2e-36,
Organism=Escherichia coli, GI1788624, Length=145, Percent_Identity=53.1034482758621, Blast_Score=172, Evalue=1e-44,
Organism=Escherichia coli, GI1789074, Length=149, Percent_Identity=33.5570469798658, Blast_Score=90, Evalue=9e-20,
Organism=Escherichia coli, GI1788834, Length=142, Percent_Identity=32.3943661971831, Blast_Score=81, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17509685, Length=132, Percent_Identity=50.7575757575758, Blast_Score=144, Evalue=3e-35,
Organism=Drosophila melanogaster, GI18859983, Length=149, Percent_Identity=45.6375838926174, Blast_Score=152, Evalue=1e-37,
Organism=Drosophila melanogaster, GI24642371, Length=149, Percent_Identity=45.6375838926174, Blast_Score=152, Evalue=1e-37,
Organism=Drosophila melanogaster, GI24651058, Length=149, Percent_Identity=45.6375838926174, Blast_Score=148, Evalue=2e-36,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NUOB_MYXXD (Q1D8S3)

Other databases:

- EMBL:   CP000113
- RefSeq:   YP_630950.1
- ProteinModelPortal:   Q1D8S3
- SMR:   Q1D8S3
- STRING:   Q1D8S3
- GeneID:   4103789
- GenomeReviews:   CP000113_GR
- KEGG:   mxa:MXAN_2733
- TIGR:   MXAN_2733
- eggNOG:   COG0377
- HOGENOM:   HBG553221
- OMA:   DLANIVT
- PhylomeDB:   Q1D8S3
- ProtClustDB:   PRK14817
- BioCyc:   MXAN246197:MXAN_2733-MONOMER
- GO:   GO:0006810
- HAMAP:   MF_01356
- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406
- PANTHER:   PTHR11995:SF2
- PANTHER:   PTHR11995
- TIGRFAMs:   TIGR01957

Pfam domain/function: PF01058 Oxidored_q6

EC number: =1.6.99.5

Molecular weight: Translated: 20871; Mature: 20740

Theoretical pI: Translated: 7.94; Mature: 7.94

Prosite motif: PS01150 COMPLEX1_20K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADADLANIVTTRRDESMGWLQSIVSKGLGWARKYSLFTYPYATACCGMEYMSVAASRHD
CCCCHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHEEEECCHHHHHHHHHHHHHHHHHHH
ISRFGAEFPRFSPRQADLLMVVGTINLKQAPILKRVYEQMAEPKWVVAFGVCASSGGFYD
HHHHHHCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCEEEEEEEECCCCCCCC
NYAVLQGIDRIIPVDVYIPGCPPRPEQVLDGLMLLQDKIGNQVHRIAEREEANPTAARHN
CHHHHHHHHHHEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHE
LLLSMNK
EEEEECC
>Mature Secondary Structure 
ADADLANIVTTRRDESMGWLQSIVSKGLGWARKYSLFTYPYATACCGMEYMSVAASRHD
CCCHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHEEEECCHHHHHHHHHHHHHHHHHHH
ISRFGAEFPRFSPRQADLLMVVGTINLKQAPILKRVYEQMAEPKWVVAFGVCASSGGFYD
HHHHHHCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCEEEEEEEECCCCCCCC
NYAVLQGIDRIIPVDVYIPGCPPRPEQVLDGLMLLQDKIGNQVHRIAEREEANPTAARHN
CHHHHHHHHHHEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHE
LLLSMNK
EEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA