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The map label for this gene is ogt [H]

Identifier: 108758006

GI number: 108758006

Start: 3045111

End: 3045668

Strand: Reverse

Name: ogt [H]

Synonym: MXAN_2611

Alternate gene names: 108758006

Gene position: 3045668-3045111 (Counterclockwise)

Preceding gene: 108760264

Following gene: 108758401

Centisome position: 33.32

GC content: 70.97

Gene sequence:

>558_bases
ATGACGATGCCCCCACCGCTGCGTTTGCTCATGGACCGCGCCCCCACGCCCATCGGCTCGATGCTGGTCGTCTGTGACGA
AGCGGGCAGCCTGCGCGCGCTCGACTGGGAGGAGTACGAGCCCCGGATGCTCCGGCTGCTGCGGCTCCACTACGGCGTGA
ACGGCTACGTGCTCGAGCCCGCGAGCGACCCCTTCGGCAGGACGACCGCGATGCAGACCTATCTGCGCGGAGACGTCAGC
GTCATCGACTCGTTGCCCGTGGCCACCGCGGGTACGCCATTCCAGCGAGAAGTCTGGCGCGCGCTGCGTGACATCCCCGC
GGGCGCCACCACGAGCTACGGCCAACTGGCCCAGCGAATCGGCCGCCCCAAGGCCGTGCGCGCCGTGGGGATGGCCAACG
GCGCCAACCCGATTGGCGTGGTGGTCCCCTGCCACCGGGTGGTGGGGGCCAACGCCTCGCTGACGGGCTACGGCGGTGGA
CTGGAGCGCAAGCAGTGGCTGCTCGACCACGAGCGCCGCCATGCATCGCGCGGGACGACGCGGCCACCAACGCGCTGA

Upstream 100 bases:

>100_bases
AGCCCCTGGCGTGCCTATGCAGCACAGCACCTCTGGGCCGCGGATGCCGCCCGGCCCGGACCCACACCCCACCTTTCCCG
TTCCTCCTCGCGACAGAGAG

Downstream 100 bases:

>100_bases
TGCCGTCGGGCGCCTCGTCCTGAAGACAGACGCCCGGCGGCATCACGCTCCGCACGCCGGTTCAGCCGCAGCCCAGCGGG
TACGTCACCTCTACCAGCGA

Product: methylated-DNA--protein-cysteine methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 185; Mature: 184

Protein sequence:

>185_residues
MTMPPPLRLLMDRAPTPIGSMLVVCDEAGSLRALDWEEYEPRMLRLLRLHYGVNGYVLEPASDPFGRTTAMQTYLRGDVS
VIDSLPVATAGTPFQREVWRALRDIPAGATTSYGQLAQRIGRPKAVRAVGMANGANPIGVVVPCHRVVGANASLTGYGGG
LERKQWLLDHERRHASRGTTRPPTR

Sequences:

>Translated_185_residues
MTMPPPLRLLMDRAPTPIGSMLVVCDEAGSLRALDWEEYEPRMLRLLRLHYGVNGYVLEPASDPFGRTTAMQTYLRGDVS
VIDSLPVATAGTPFQREVWRALRDIPAGATTSYGQLAQRIGRPKAVRAVGMANGANPIGVVVPCHRVVGANASLTGYGGG
LERKQWLLDHERRHASRGTTRPPTR
>Mature_184_residues
TMPPPLRLLMDRAPTPIGSMLVVCDEAGSLRALDWEEYEPRMLRLLRLHYGVNGYVLEPASDPFGRTTAMQTYLRGDVSV
IDSLPVATAGTPFQREVWRALRDIPAGATTSYGQLAQRIGRPKAVRAVGMANGANPIGVVVPCHRVVGANASLTGYGGGL
ERKQWLLDHERRHASRGTTRPPTR

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=116, Percent_Identity=38.7931034482759, Blast_Score=82, Evalue=3e-16,
Organism=Escherichia coli, GI1787596, Length=165, Percent_Identity=55.7575757575758, Blast_Score=205, Evalue=1e-54,
Organism=Escherichia coli, GI1788542, Length=124, Percent_Identity=42.741935483871, Blast_Score=103, Evalue=8e-24,
Organism=Caenorhabditis elegans, GI115533070, Length=92, Percent_Identity=42.3913043478261, Blast_Score=78, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI115533068, Length=92, Percent_Identity=42.3913043478261, Blast_Score=78, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6320001, Length=85, Percent_Identity=45.8823529411765, Blast_Score=94, Evalue=1e-20,
Organism=Drosophila melanogaster, GI17137554, Length=98, Percent_Identity=43.8775510204082, Blast_Score=74, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 20242; Mature: 20110

Theoretical pI: Translated: 10.25; Mature: 10.25

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMPPPLRLLMDRAPTPIGSMLVVCDEAGSLRALDWEEYEPRMLRLLRLHYGVNGYVLEP
CCCCCHHHHHHHCCCCCCCCEEEEECCCCCEEECCCHHCCHHHHHHHHHHCCCCCEEECC
ASDPFGRTTAMQTYLRGDVSVIDSLPVATAGTPFQREVWRALRDIPAGATTSYGQLAQRI
CCCCCCHHHHHHHHHHCCHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHH
GRPKAVRAVGMANGANPIGVVVPCHRVVGANASLTGYGGGLERKQWLLDHERRHASRGTT
CCCHHHHHHHCCCCCCCEEEEEECHHHHCCCCCEECCCCCCCHHHHHHHHHHHHHHCCCC
RPPTR
CCCCC
>Mature Secondary Structure 
TMPPPLRLLMDRAPTPIGSMLVVCDEAGSLRALDWEEYEPRMLRLLRLHYGVNGYVLEP
CCCCHHHHHHHCCCCCCCCEEEEECCCCCEEECCCHHCCHHHHHHHHHHCCCCCEEECC
ASDPFGRTTAMQTYLRGDVSVIDSLPVATAGTPFQREVWRALRDIPAGATTSYGQLAQRI
CCCCCCHHHHHHHHHHCCHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHH
GRPKAVRAVGMANGANPIGVVVPCHRVVGANASLTGYGGGLERKQWLLDHERRHASRGTT
CCCHHHHHHHCCCCCCCEEEEEECHHHHCCCCCEECCCCCCCHHHHHHHHHHHHHHCCCC
RPPTR
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2825131; 9097039; 9278503; 1520330 [H]