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The map label for this gene is kinE [H]

Identifier: 108757873

GI number: 108757873

Start: 2774506

End: 2776797

Strand: Reverse

Name: kinE [H]

Synonym: MXAN_2386

Alternate gene names: 108757873

Gene position: 2776797-2774506 (Counterclockwise)

Preceding gene: 108763756

Following gene: 108763699

Centisome position: 30.38

GC content: 70.11

Gene sequence:

>2292_bases
ATGCGGGTCCTGATTGTCACCCAGGGGGGAGGGGCGGACCTGGCGCCAGTCGAGTCCTGGCTGCGTGAGCAGGGACACAC
GGTGGTCACCGTGACGCGCGAGGCGGAGGTGCCCGCCGCGTGGCGCAGCGGAACGTGCGCGCTGGTGATGGTGGATGCCC
GGACGGACCCCTCCCGCGTCCCGCTGGTGCGCGCACTTCGCAGCCTGCCCGGCGGTGAGGAATCCGTGTTGCTCCTCCTG
GGCCACCGGGGCGCGCTGGGCGGACTGCGGCCCGTCCTGGAGGCCGGCGCCGATGATTTGCTCGCGTGGCCCGTGGATGA
GGAGGAACTGGCGCTGCGGTTGGAGCTGGCCCACCGCCGCTTCGTCCGGCGCGAGGGCCGCAAGGGCCTGCCCTTCGGAG
ATGACCTGAAGGAGACGCTGCTGGCCGTCAGCCCCGTGCCCACGTCCATCACCACCATCACCGAGGGGCGCGTGGTGGCG
GCCAACGAAGCCTACTTCGAACTCTTCGGCTACACGCGCGAGGAGGTCATCGGGCGCACCACGATGGAGCTGGCGCTGTG
GCAGGCGCCCCTGAACCGCTCGCAAATCGTGGACACGCTGCGGCGCCACGGCACGGTGCGCGGCGTGGACGCGCAGTACC
GCACCCGCGAGGGCGAGCTGCGGCACACGTTGCTCTTCATGGGCCTGGTGCCCTACGGCGGCACGCCCCACATCATCGCC
TTCTTCCCGGACATCACCCCGCTCAAGCGCGCCGAGGAGAGCCTGCGCCGCTCGGAGGTGAGCTTCCGCACCCTCATCGG
CAGCCTGCCGGACATGGTGGCCGTCTTCGACACCGACTCGCGCGTGCGCTACGCCAACCTCAAGGTCGCCACGGCCCTGG
GCTACGAGAGTGCGAACGAGCTGCTGGGCAAGCACTTCTCCGACATCATCCCCAAGGAGGACTTCGCCAGCGCCAACGCG
CGCATGCACGAAGCCATCCGCACCGGCCGCGCGGCGCACCAGGAGCGGCGGCTGCTCAAACGCGACGGCAGCGTGCTGCA
CGTGGAGTCCACCACCTTTCCCCTGCCCTTCGACGGCGAGGACTCCATCGTTGCCGTCTCCCATGACTTGACGGAGCGCT
ACCAGATGCAGGCGCGGCTGATGCTCGCGCAGCGCATGGCCTCCGTCGGCACACTGGCCGCGGGCGTGGCACACGAAATC
AACAACCCCCTGGCCTACCTCACCGCCAACCTGGCCTTCGCACGCGAGGAGCTCACCGGCGTGCTGCCCACCGGCACCCG
GAACATGGAGCCCCGGCTGGCGGAGGCGGTGGCCAGCGCCCAGGCCGCGCTCGCCGAGGCGCAGCAGGGCGCCGACCGGG
TGCGCAGCATCGTCCGGGACTTGAAGACGTTCAGCCGGGTGGACTCAGCGGAGAGCGAGGAAGTGGACGTCCGGCAGGTG
CTGGAGTCCACGCTGAACCTGGCCACCACGGAGATTCGTCACCGCGCCCGCCTGGTGAAGCAGCTCGACGCGGTCCCCAC
CGTGGTCGGCAACGAGTCACGGTTGGGACAGGTGTTCCTCAACCTGCTGGTGAACGCCGCCCAGGCCATTCCCTCCGGGA
CGCCGGAGCGCCATGAAATCCGCGTCGTCACCCGCCTTTCGGGCAACGGCCACGTCTGCGTGGAGGTGTCGGACACGGGC
GCCGGTATCGCCAAGGAACACCTGCCGCGCCTGTTCGACCCCTTCTTCACCACCAAGGAGCCCGGGGTGGGCACCGGACT
GGGCCTGTCCATCTGCCACAGCCTGGTGACGGCCCTGGGTGGGGAAATCCATGCGACGAGCGAGCCGGGCAGGGGCTCCA
CCTTCCAGGTGCTCCTGACCCCCTCCCAGCGACTCGGGACCGAGCGCCCACCGCCCGCTCCGCCGCCCGCCCCCACGGAG
AAACGGGGCAGCGTGCTGGTGGTGGACGACGAGCCGCTCGTCTGCACGGCCCTGGGGCGTACGCTGCGTCCGCACCACGA
CGTCACCCTCTTCACCCGGGCCCAGGAGGCCTTGGAGCGCATCGAGGCCGGCGAGCGCTTCGACGTCGTCTTCTGCGACC
TGATGATGCCGGGCATGAGTGGGATGGACTTCTACTCCGCCCTCCAGGCCCGCCACCCCGAGCAGGCCCAGCGGGTCATC
TTCCTCACCGGCGGCGCGGTGACGCCTCAGGCCCGCGCCTTCCTGGAATCGGTACCAAGCCCCCATCTGGAAAAGCCGTT
CGCTGGCAGGGAACTCCTCTCGCTGATCCAGGAACGGCTCGCGCACGCATAG

Upstream 100 bases:

>100_bases
CCGGGCGCTTCGTCACCTGCACGCCACACCGGCACGGCACCCCGCTTTTACAACAGCAAACGTCGTGTAACGATGAGCGT
TCGTCATCAAGAGGTGTCCC

Downstream 100 bases:

>100_bases
CGAATTGTCAGCCTGTGGAAGATTTGGACAGGCCCCCCCACCCCCTGCCCCTATCCTCCCCACCGCCCCCGACCGCTGGG
CAGGAAACCGTCCACGGGAG

Product: putative sensory box histidine kinase/response regulator

Products: NA

Alternate protein names: Sensor histidine kinase E [H]

Number of amino acids: Translated: 763; Mature: 763

Protein sequence:

>763_residues
MRVLIVTQGGGADLAPVESWLREQGHTVVTVTREAEVPAAWRSGTCALVMVDARTDPSRVPLVRALRSLPGGEESVLLLL
GHRGALGGLRPVLEAGADDLLAWPVDEEELALRLELAHRRFVRREGRKGLPFGDDLKETLLAVSPVPTSITTITEGRVVA
ANEAYFELFGYTREEVIGRTTMELALWQAPLNRSQIVDTLRRHGTVRGVDAQYRTREGELRHTLLFMGLVPYGGTPHIIA
FFPDITPLKRAEESLRRSEVSFRTLIGSLPDMVAVFDTDSRVRYANLKVATALGYESANELLGKHFSDIIPKEDFASANA
RMHEAIRTGRAAHQERRLLKRDGSVLHVESTTFPLPFDGEDSIVAVSHDLTERYQMQARLMLAQRMASVGTLAAGVAHEI
NNPLAYLTANLAFAREELTGVLPTGTRNMEPRLAEAVASAQAALAEAQQGADRVRSIVRDLKTFSRVDSAESEEVDVRQV
LESTLNLATTEIRHRARLVKQLDAVPTVVGNESRLGQVFLNLLVNAAQAIPSGTPERHEIRVVTRLSGNGHVCVEVSDTG
AGIAKEHLPRLFDPFFTTKEPGVGTGLGLSICHSLVTALGGEIHATSEPGRGSTFQVLLTPSQRLGTERPPPAPPPAPTE
KRGSVLVVDDEPLVCTALGRTLRPHHDVTLFTRAQEALERIEAGERFDVVFCDLMMPGMSGMDFYSALQARHPEQAQRVI
FLTGGAVTPQARAFLESVPSPHLEKPFAGRELLSLIQERLAHA

Sequences:

>Translated_763_residues
MRVLIVTQGGGADLAPVESWLREQGHTVVTVTREAEVPAAWRSGTCALVMVDARTDPSRVPLVRALRSLPGGEESVLLLL
GHRGALGGLRPVLEAGADDLLAWPVDEEELALRLELAHRRFVRREGRKGLPFGDDLKETLLAVSPVPTSITTITEGRVVA
ANEAYFELFGYTREEVIGRTTMELALWQAPLNRSQIVDTLRRHGTVRGVDAQYRTREGELRHTLLFMGLVPYGGTPHIIA
FFPDITPLKRAEESLRRSEVSFRTLIGSLPDMVAVFDTDSRVRYANLKVATALGYESANELLGKHFSDIIPKEDFASANA
RMHEAIRTGRAAHQERRLLKRDGSVLHVESTTFPLPFDGEDSIVAVSHDLTERYQMQARLMLAQRMASVGTLAAGVAHEI
NNPLAYLTANLAFAREELTGVLPTGTRNMEPRLAEAVASAQAALAEAQQGADRVRSIVRDLKTFSRVDSAESEEVDVRQV
LESTLNLATTEIRHRARLVKQLDAVPTVVGNESRLGQVFLNLLVNAAQAIPSGTPERHEIRVVTRLSGNGHVCVEVSDTG
AGIAKEHLPRLFDPFFTTKEPGVGTGLGLSICHSLVTALGGEIHATSEPGRGSTFQVLLTPSQRLGTERPPPAPPPAPTE
KRGSVLVVDDEPLVCTALGRTLRPHHDVTLFTRAQEALERIEAGERFDVVFCDLMMPGMSGMDFYSALQARHPEQAQRVI
FLTGGAVTPQARAFLESVPSPHLEKPFAGRELLSLIQERLAHA
>Mature_763_residues
MRVLIVTQGGGADLAPVESWLREQGHTVVTVTREAEVPAAWRSGTCALVMVDARTDPSRVPLVRALRSLPGGEESVLLLL
GHRGALGGLRPVLEAGADDLLAWPVDEEELALRLELAHRRFVRREGRKGLPFGDDLKETLLAVSPVPTSITTITEGRVVA
ANEAYFELFGYTREEVIGRTTMELALWQAPLNRSQIVDTLRRHGTVRGVDAQYRTREGELRHTLLFMGLVPYGGTPHIIA
FFPDITPLKRAEESLRRSEVSFRTLIGSLPDMVAVFDTDSRVRYANLKVATALGYESANELLGKHFSDIIPKEDFASANA
RMHEAIRTGRAAHQERRLLKRDGSVLHVESTTFPLPFDGEDSIVAVSHDLTERYQMQARLMLAQRMASVGTLAAGVAHEI
NNPLAYLTANLAFAREELTGVLPTGTRNMEPRLAEAVASAQAALAEAQQGADRVRSIVRDLKTFSRVDSAESEEVDVRQV
LESTLNLATTEIRHRARLVKQLDAVPTVVGNESRLGQVFLNLLVNAAQAIPSGTPERHEIRVVTRLSGNGHVCVEVSDTG
AGIAKEHLPRLFDPFFTTKEPGVGTGLGLSICHSLVTALGGEIHATSEPGRGSTFQVLLTPSQRLGTERPPPAPPPAPTE
KRGSVLVVDDEPLVCTALGRTLRPHHDVTLFTRAQEALERIEAGERFDVVFCDLMMPGMSGMDFYSALQARHPEQAQRVI
FLTGGAVTPQARAFLESVPSPHLEKPFAGRELLSLIQERLAHA

Specific function: Phosphorylates the sporulation-regulatory protein spo0A under biofilm growth conditions. Also able to weakly phosphorylate spo0F [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 PAS (PER-ARNT-SIM) domains [H]

Homologues:

Organism=Escherichia coli, GI1788549, Length=258, Percent_Identity=31.0077519379845, Blast_Score=126, Evalue=6e-30,
Organism=Escherichia coli, GI1790436, Length=252, Percent_Identity=32.5396825396825, Blast_Score=115, Evalue=9e-27,
Organism=Escherichia coli, GI48994928, Length=536, Percent_Identity=23.5074626865672, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI145693157, Length=280, Percent_Identity=31.0714285714286, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI87081816, Length=269, Percent_Identity=29.7397769516729, Blast_Score=91, Evalue=3e-19,
Organism=Escherichia coli, GI1790300, Length=366, Percent_Identity=28.9617486338798, Blast_Score=86, Evalue=1e-17,
Organism=Escherichia coli, GI1786600, Length=376, Percent_Identity=25, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI1789149, Length=248, Percent_Identity=29.0322580645161, Blast_Score=74, Evalue=4e-14,
Organism=Escherichia coli, GI1788713, Length=207, Percent_Identity=27.0531400966184, Blast_Score=73, Evalue=8e-14,
Organism=Escherichia coli, GI1788393, Length=273, Percent_Identity=27.8388278388278, Blast_Score=66, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 83369; Mature: 83369

Theoretical pI: Translated: 6.48; Mature: 6.48

Prosite motif: PS50112 PAS ; PS50113 PAC ; PS50110 RESPONSE_REGULATORY ; PS50109 HIS_KIN ; PS00133 CARBOXYPEPT_ZN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVLIVTQGGGADLAPVESWLREQGHTVVTVTREAEVPAAWRSGTCALVMVDARTDPSRV
CEEEEEECCCCCCHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCEEEEEEECCCCCCCC
PLVRALRSLPGGEESVLLLLGHRGALGGLRPVLEAGADDLLAWPVDEEELALRLELAHRR
HHHHHHHHCCCCCCEEEEEEECCCCCCCHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHH
FVRREGRKGLPFGDDLKETLLAVSPVPTSITTITEGRVVAANEAYFELFGYTREEVIGRT
HHHHCCCCCCCCCCCHHHHHHCCCCCCCCEEEEECCEEEEECHHHHHHHCCCHHHHHCHH
TMELALWQAPLNRSQIVDTLRRHGTVRGVDAQYRTREGELRHTLLFMGLVPYGGTPHIIA
HHHHHHHHCCCCHHHHHHHHHHCCCEECCCCHHHCCCCHHHHHHHHHHHCCCCCCCCEEE
FFPDITPLKRAEESLRRSEVSFRTLIGSLPDMVAVFDTDSRVRYANLKVATALGYESANE
ECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEEECEEEEEHHCHHHHHH
LLGKHFSDIIPKEDFASANARMHEAIRTGRAAHQERRLLKRDGSVLHVESTTFPLPFDGE
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCC
DSIVAVSHDLTERYQMQARLMLAQRMASVGTLAAGVAHEINNPLAYLTANLAFAREELTG
CCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCE
VLPTGTRNMEPRLAEAVASAQAALAEAQQGADRVRSIVRDLKTFSRVDSAESEEVDVRQV
ECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
LESTLNLATTEIRHRARLVKQLDAVPTVVGNESRLGQVFLNLLVNAAQAIPSGTPERHEI
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEE
RVVTRLSGNGHVCVEVSDTGAGIAKEHLPRLFDPFFTTKEPGVGTGLGLSICHSLVTALG
EEEEEECCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCC
GEIHATSEPGRGSTFQVLLTPSQRLGTERPPPAPPPAPTEKRGSVLVVDDEPLVCTALGR
CCEEECCCCCCCCEEEEEECCHHHCCCCCCCCCCCCCCCCCCCCEEEECCCCEEHHHHCC
TLRPHHDVTLFTRAQEALERIEAGERFDVVFCDLMMPGMSGMDFYSALQARHPEQAQRVI
CCCCCCCEEEHHHHHHHHHHHHCCCCEEEEEEEHHCCCCCCCHHHHHHHHCCCCCCCEEE
FLTGGAVTPQARAFLESVPSPHLEKPFAGRELLSLIQERLAHA
EEECCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRVLIVTQGGGADLAPVESWLREQGHTVVTVTREAEVPAAWRSGTCALVMVDARTDPSRV
CEEEEEECCCCCCHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCEEEEEEECCCCCCCC
PLVRALRSLPGGEESVLLLLGHRGALGGLRPVLEAGADDLLAWPVDEEELALRLELAHRR
HHHHHHHHCCCCCCEEEEEEECCCCCCCHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHH
FVRREGRKGLPFGDDLKETLLAVSPVPTSITTITEGRVVAANEAYFELFGYTREEVIGRT
HHHHCCCCCCCCCCCHHHHHHCCCCCCCCEEEEECCEEEEECHHHHHHHCCCHHHHHCHH
TMELALWQAPLNRSQIVDTLRRHGTVRGVDAQYRTREGELRHTLLFMGLVPYGGTPHIIA
HHHHHHHHCCCCHHHHHHHHHHCCCEECCCCHHHCCCCHHHHHHHHHHHCCCCCCCCEEE
FFPDITPLKRAEESLRRSEVSFRTLIGSLPDMVAVFDTDSRVRYANLKVATALGYESANE
ECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEEECEEEEEHHCHHHHHH
LLGKHFSDIIPKEDFASANARMHEAIRTGRAAHQERRLLKRDGSVLHVESTTFPLPFDGE
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCC
DSIVAVSHDLTERYQMQARLMLAQRMASVGTLAAGVAHEINNPLAYLTANLAFAREELTG
CCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCE
VLPTGTRNMEPRLAEAVASAQAALAEAQQGADRVRSIVRDLKTFSRVDSAESEEVDVRQV
ECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
LESTLNLATTEIRHRARLVKQLDAVPTVVGNESRLGQVFLNLLVNAAQAIPSGTPERHEI
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEE
RVVTRLSGNGHVCVEVSDTGAGIAKEHLPRLFDPFFTTKEPGVGTGLGLSICHSLVTALG
EEEEEECCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCC
GEIHATSEPGRGSTFQVLLTPSQRLGTERPPPAPPPAPTEKRGSVLVVDDEPLVCTALGR
CCEEECCCCCCCCEEEEEECCHHHCCCCCCCCCCCCCCCCCCCCEEEECCCCEEHHHHCC
TLRPHHDVTLFTRAQEALERIEAGERFDVVFCDLMMPGMSGMDFYSALQARHPEQAQRVI
CCCCCCCEEEHHHHHHHHHHHHCCCCEEEEEEEHHCCCCCCCHHHHHHHHCCCCCCCEEE
FLTGGAVTPQARAFLESVPSPHLEKPFAGRELLSLIQERLAHA
EEECCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]