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The map label for this gene is cls [H]

Identifier: 108757767

GI number: 108757767

Start: 127779

End: 129026

Strand: Direct

Name: cls [H]

Synonym: MXAN_0110

Alternate gene names: 108757767

Gene position: 127779-129026 (Clockwise)

Preceding gene: 108761237

Following gene: 108759336

Centisome position: 1.4

GC content: 67.95

Gene sequence:

>1248_bases
GTGAAGCTCGCCTGGCTCGTGCCCGTGGTGTTGATTGCCGCCGGGTGTCCCGCTCCCAATCACCGGCATGACCTGCGCCT
GCGCGCGTTGCCGGGGACCAGTCCGGAGGCGCGCTCGCTCGCGTTCTTCCAGTCATTGGGCGTGGCGCTGGTGCCAGGGC
ACCGCGTGGAGTTGGTGGAGAACGGCTTCATCTTCGACGCCATCGAGGAGGAGATTCTCGCCGCGCGCACCAGCATCCAC
ATCATCAGCTACATCTGGCGCCCCGGTGAGCCGTCGGACCGGCTGGTGCGGGCGCTGGCGGCGCGGCGGCCCGGGGTGGC
GTGCCGCGTGCTCGTGGACCCATTGGGCAGCGTCAACTTCGAGGCGGTGATTCCCGCGCTGGCGGCCAGCGGCTGTGACG
CGCGCTACTTCCGGCCCATCCAAGGTGACTTCGTGGCCTTGGACGCGGTTCGCCTGAGGGCGCGGAACCACCGGAAGATG
GTGGTGAGGGATGGCGAGGTGGGCATCACCGGCGGCTGGGGCATCTGGAAGAGCTGGATGGGGAATGCGCAGAGCGCGGA
GTCCTGGCGCGACATGAACATCCGCGTGGAGGGCCCGGCCGTGCGAGAGATGCAGGTCGCCTTCGCGCAGAACTGGCAGG
AGGCGGGTGGAGACTTCCTGCCGGCGACGGACTTCCCGGAGCCTCGCTACGCGGGGGATGCGCGCGCCGGCTTCGTGGCC
AGCACGGACAACCGCTTCCTGTCGGACGCGCGGCGGATGACGCTGCTCACCATCGCGGCGGCGCAGGAGCGGCTCTGGAT
TGCGAACTCCTACTTCATTCCCTCCGACGCCATCAGTGACATGCTGCTGGAGAAGGTGAAGCAAGGCGTGGACGTGCGGG
TGCTGGTGCCCGGGCGGCACCATGACGTCGGGCCCGTGAACGCGGCGCAGCGGGCGTCCTATGCGCGCTTGCTGGAGGGC
GGCGTGCGCATCTGGGAGTACGAGCTGTCGATGATGCATTCCAAGACGATGCTCGTGGATGACTCCCTGTCCGTCGTGGG
CTCCACCAACATGGACCCGCTGGCGTTGACGACGTTGGAGGAGTGCTCGGTGGTGGTGGAGGACGTGGCGCTGGCGGCGC
AGCTCGCCGCATCCTTTGAGAAGGATTTACGCCACTCGCGGGAGATTCACTGGAGCGGGTGGAAGCGGCGCGGGCTCTTG
CAGAAGCTGGGCGAGCGGCTGCCGTGGTTCATCGGCAGTTTTCTCTGA

Upstream 100 bases:

>100_bases
TCCACCCTCACGGTGGAGAGGTGCCCGCCGCGCGTCTCGGGGTGTCTTGCGTGAGACATCGGCAGGTGCGCCGCGGGCCA
TGGGCGCTTAGTTTCCGGCG

Downstream 100 bases:

>100_bases
GGCGTGGTTCCACGCTCGGAGTGTATGGGGAGGGGATGGTAAAGAGGCGCCATGGCTCGCGCACGCCCGACCTCGCCTCG
TTGCCCTCGTTGTAACGCGC

Product: cardiolipin synthase

Products: Biphosphatidylglycerol; Glycerol. [C]

Alternate protein names: CL synthase 1 [H]

Number of amino acids: Translated: 415; Mature: 415

Protein sequence:

>415_residues
MKLAWLVPVVLIAAGCPAPNHRHDLRLRALPGTSPEARSLAFFQSLGVALVPGHRVELVENGFIFDAIEEEILAARTSIH
IISYIWRPGEPSDRLVRALAARRPGVACRVLVDPLGSVNFEAVIPALAASGCDARYFRPIQGDFVALDAVRLRARNHRKM
VVRDGEVGITGGWGIWKSWMGNAQSAESWRDMNIRVEGPAVREMQVAFAQNWQEAGGDFLPATDFPEPRYAGDARAGFVA
STDNRFLSDARRMTLLTIAAAQERLWIANSYFIPSDAISDMLLEKVKQGVDVRVLVPGRHHDVGPVNAAQRASYARLLEG
GVRIWEYELSMMHSKTMLVDDSLSVVGSTNMDPLALTTLEECSVVVEDVALAAQLAASFEKDLRHSREIHWSGWKRRGLL
QKLGERLPWFIGSFL

Sequences:

>Translated_415_residues
MKLAWLVPVVLIAAGCPAPNHRHDLRLRALPGTSPEARSLAFFQSLGVALVPGHRVELVENGFIFDAIEEEILAARTSIH
IISYIWRPGEPSDRLVRALAARRPGVACRVLVDPLGSVNFEAVIPALAASGCDARYFRPIQGDFVALDAVRLRARNHRKM
VVRDGEVGITGGWGIWKSWMGNAQSAESWRDMNIRVEGPAVREMQVAFAQNWQEAGGDFLPATDFPEPRYAGDARAGFVA
STDNRFLSDARRMTLLTIAAAQERLWIANSYFIPSDAISDMLLEKVKQGVDVRVLVPGRHHDVGPVNAAQRASYARLLEG
GVRIWEYELSMMHSKTMLVDDSLSVVGSTNMDPLALTTLEECSVVVEDVALAAQLAASFEKDLRHSREIHWSGWKRRGLL
QKLGERLPWFIGSFL
>Mature_415_residues
MKLAWLVPVVLIAAGCPAPNHRHDLRLRALPGTSPEARSLAFFQSLGVALVPGHRVELVENGFIFDAIEEEILAARTSIH
IISYIWRPGEPSDRLVRALAARRPGVACRVLVDPLGSVNFEAVIPALAASGCDARYFRPIQGDFVALDAVRLRARNHRKM
VVRDGEVGITGGWGIWKSWMGNAQSAESWRDMNIRVEGPAVREMQVAFAQNWQEAGGDFLPATDFPEPRYAGDARAGFVA
STDNRFLSDARRMTLLTIAAAQERLWIANSYFIPSDAISDMLLEKVKQGVDVRVLVPGRHHDVGPVNAAQRASYARLLEG
GVRIWEYELSMMHSKTMLVDDSLSVVGSTNMDPLALTTLEECSVVVEDVALAAQLAASFEKDLRHSREIHWSGWKRRGLL
QKLGERLPWFIGSFL

Specific function: Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol [H]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787502, Length=379, Percent_Identity=30.0791556728232, Blast_Score=161, Evalue=9e-41,
Organism=Escherichia coli, GI1787007, Length=322, Percent_Identity=29.5031055900621, Blast_Score=114, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022924
- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.- [C]

Molecular weight: Translated: 45951; Mature: 45951

Theoretical pI: Translated: 7.33; Mature: 7.33

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLAWLVPVVLIAAGCPAPNHRHDLRLRALPGTSPEARSLAFFQSLGVALVPGHRVELVE
CCHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHCCEEEECCCEEEEEE
NGFIFDAIEEEILAARTSIHIISYIWRPGEPSDRLVRALAARRPGVACRVLVDPLGSVNF
CCCCHHHHHHHHHHHHHHHEEEHEEECCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCH
EAVIPALAASGCDARYFRPIQGDFVALDAVRLRARNHRKMVVRDGEVGITGGWGIWKSWM
HHHHHHHHHCCCCCHHCCCCCCCEEEHHHHHHHHCCCCEEEEECCCEEEECCHHHHHHHC
GNAQSAESWRDMNIRVEGPAVREMQVAFAQNWQEAGGDFLPATDFPEPRYAGDARAGFVA
CCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEE
STDNRFLSDARRMTLLTIAAAQERLWIANSYFIPSDAISDMLLEKVKQGVDVRVLVPGRH
CCCCHHHHHHHHHHEEEEEHHHCEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEECCCC
HDVGPVNAAQRASYARLLEGGVRIWEYELSMMHSKTMLVDDSLSVVGSTNMDPLALTTLE
CCCCCCCHHHHHHHHHHHHCCCEEEEEHHHHHHCCEEEEECCCCEEECCCCCCEEEHHHH
ECSVVVEDVALAAQLAASFEKDLRHSREIHWSGWKRRGLLQKLGERLPWFIGSFL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCHHHHHCC
>Mature Secondary Structure
MKLAWLVPVVLIAAGCPAPNHRHDLRLRALPGTSPEARSLAFFQSLGVALVPGHRVELVE
CCHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHCCEEEECCCEEEEEE
NGFIFDAIEEEILAARTSIHIISYIWRPGEPSDRLVRALAARRPGVACRVLVDPLGSVNF
CCCCHHHHHHHHHHHHHHHEEEHEEECCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCH
EAVIPALAASGCDARYFRPIQGDFVALDAVRLRARNHRKMVVRDGEVGITGGWGIWKSWM
HHHHHHHHHCCCCCHHCCCCCCCEEEHHHHHHHHCCCCEEEEECCCEEEECCHHHHHHHC
GNAQSAESWRDMNIRVEGPAVREMQVAFAQNWQEAGGDFLPATDFPEPRYAGDARAGFVA
CCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEE
STDNRFLSDARRMTLLTIAAAQERLWIANSYFIPSDAISDMLLEKVKQGVDVRVLVPGRH
CCCCHHHHHHHHHHEEEEEHHHCEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEECCCC
HDVGPVNAAQRASYARLLEGGVRIWEYELSMMHSKTMLVDDSLSVVGSTNMDPLALTTLE
CCCCCCCHHHHHHHHHHHHCCCEEEEEHHHHHHCCEEEEECCCCEEECCCCCCEEEHHHH
ECSVVVEDVALAAQLAASFEKDLRHSREIHWSGWKRRGLLQKLGERLPWFIGSFL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Phosphatidylglycerol [C]

Specific reaction: 2 Phosphatidylglycerol = Biphosphatidylglycerol + Glycerol. [C]

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]