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The map label for this gene is rpfG [H]

Identifier: 108757216

GI number: 108757216

Start: 5191503

End: 5192738

Strand: Direct

Name: rpfG [H]

Synonym: MXAN_4232

Alternate gene names: 108757216

Gene position: 5191503-5192738 (Clockwise)

Preceding gene: 108760745

Following gene: 108757868

Centisome position: 56.8

GC content: 67.15

Gene sequence:

>1236_bases
GTGGAAGCCATCCCCCCTGCCCCACCCCGAATCCTCATCGTCGATGATGACGACTCCGTACGAGACGTCATCTCTGTCCT
GCTACGTGAGGAAGGCTACAACTGTGTCGTCGCCAACGGGGCCGAGATGGCCCTGGACCTGGCGGGCGAGGAGGAGACGC
CGCTCGTCATCAGTGACATGAAGATGCCGGGCAAGGACGGCCTCTGGCTCCTGGAGAACCTCCGCGAGCGGCTCCCCGAC
ACCTCCGTCATCATGCTCACGGGCTACGGTGACACCGAATCGGCAGTGGACTGCCTGCGCCGCGGCGCGGTGGACTACCT
GCTCAAGCCACCCAAGCTCACGGACCTCATCCGGGCCATCGAGCGGGCGCTGGCCAAGCGCCGCATCGAGATGGCCCGCA
AGCGCTACCAGAAGAAGCTGGAGCGCAAGGTCCGGGACAGGACGGCCGAGCTGCGCAGCGCCCTGCGCGACATCGCCAAC
ACGTACCAGAACACGCTGCTGGCCCTGGTGGCCGCCCTGGACGCGCGCGAGCACGAGACGAGCGACCACTCCCAGCGCGT
GGTCAGCTACACGTCCGCCATCGCGCAGCGCATGGGCATCCAGGGCAAGGAGCTGGAGGAGATCGGACGCGGCGCGCTGC
TGCACGACATCGGGAAGATTGGCGTGCCGGACGCGGTGCTGCTCAAGCCGGGCAAGCTCACCCCGGACGAGTGGCTGGAG
ATGCGCAAGCACCCGGACATCGGCTTCCAGATGATCCAGGCCATTCCGTTCCTGGACACGCCTGCCTCCATCGTCCTTTC
GCACCAGGAGCGCTGGGACGGCGCCGGCTATCCGCGCAACCTCCAGCGGCACGAAATCCACATCGGCGCGCGCATCTTCG
CCGTGGCGGACACGCTGGACGCGATGACGAGCGACCGGCCCTACCGCAAGGGCACGACGTTCACCAACGCCATCCAGGAG
ATCAAACGCTGCGCCAACACGCAGTTCGATCCGGAGGTCGTGCGGGCGTTCCTCGACATTGGCGAGGAGGGGCTGATCCG
CATCAAGCAGGAGATGGCGACGAAGAAGCTCCAGCTCCCGCAGGCCGAGCAGGACGCCCATGACGCCGAGGCGGAGCTGG
CCCGGCTCACGGATCTGGACGACGACGTGGACGCCGCCGTGCCTGGGCACTCCGCCAGTGACGAGGACGAGCCCAAGTCC
GCCGTCGTTCGTTCGGTGGCTGGCAACAAGGGGTGA

Upstream 100 bases:

>100_bases
TCGCCAGGCCCCTCAATCGATCAGCCGTGAACCCTGATCCGATGGTAAATTCCCGTACGCGTTGGCCAATGAATCCCGGA
AACCTCTAGAATCGCTCGCT

Downstream 100 bases:

>100_bases
GCCTGGCTGGAAGAGATGACCGCACGGGGGGCGGCTGTTATGAGGACAGGTCCGCCGTGACGACACCTGCCCTCCAGCCC
CCGCCTTCCGCGTTGGCTCC

Product: response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 411; Mature: 411

Protein sequence:

>411_residues
MEAIPPAPPRILIVDDDDSVRDVISVLLREEGYNCVVANGAEMALDLAGEEETPLVISDMKMPGKDGLWLLENLRERLPD
TSVIMLTGYGDTESAVDCLRRGAVDYLLKPPKLTDLIRAIERALAKRRIEMARKRYQKKLERKVRDRTAELRSALRDIAN
TYQNTLLALVAALDAREHETSDHSQRVVSYTSAIAQRMGIQGKELEEIGRGALLHDIGKIGVPDAVLLKPGKLTPDEWLE
MRKHPDIGFQMIQAIPFLDTPASIVLSHQERWDGAGYPRNLQRHEIHIGARIFAVADTLDAMTSDRPYRKGTTFTNAIQE
IKRCANTQFDPEVVRAFLDIGEEGLIRIKQEMATKKLQLPQAEQDAHDAEAELARLTDLDDDVDAAVPGHSASDEDEPKS
AVVRSVAGNKG

Sequences:

>Translated_411_residues
MEAIPPAPPRILIVDDDDSVRDVISVLLREEGYNCVVANGAEMALDLAGEEETPLVISDMKMPGKDGLWLLENLRERLPD
TSVIMLTGYGDTESAVDCLRRGAVDYLLKPPKLTDLIRAIERALAKRRIEMARKRYQKKLERKVRDRTAELRSALRDIAN
TYQNTLLALVAALDAREHETSDHSQRVVSYTSAIAQRMGIQGKELEEIGRGALLHDIGKIGVPDAVLLKPGKLTPDEWLE
MRKHPDIGFQMIQAIPFLDTPASIVLSHQERWDGAGYPRNLQRHEIHIGARIFAVADTLDAMTSDRPYRKGTTFTNAIQE
IKRCANTQFDPEVVRAFLDIGEEGLIRIKQEMATKKLQLPQAEQDAHDAEAELARLTDLDDDVDAAVPGHSASDEDEPKS
AVVRSVAGNKG
>Mature_411_residues
MEAIPPAPPRILIVDDDDSVRDVISVLLREEGYNCVVANGAEMALDLAGEEETPLVISDMKMPGKDGLWLLENLRERLPD
TSVIMLTGYGDTESAVDCLRRGAVDYLLKPPKLTDLIRAIERALAKRRIEMARKRYQKKLERKVRDRTAELRSALRDIAN
TYQNTLLALVAALDAREHETSDHSQRVVSYTSAIAQRMGIQGKELEEIGRGALLHDIGKIGVPDAVLLKPGKLTPDEWLE
MRKHPDIGFQMIQAIPFLDTPASIVLSHQERWDGAGYPRNLQRHEIHIGARIFAVADTLDAMTSDRPYRKGTTFTNAIQE
IKRCANTQFDPEVVRAFLDIGEEGLIRIKQEMATKKLQLPQAEQDAHDAEAELARLTDLDDDVDAAVPGHSASDEDEPKS
AVVRSVAGNKG

Specific function: Positively regulates the synthesis of extracellular enzymes and extracellular polysaccharides. The cell-cell signaling system encoded by rpf genes is required for full virulence and is implicated in regulation of the formation and dispersal of biofilms [H

COG id: COG3437

COG function: function code KT; Response regulator containing a CheY-like receiver domain and an HD-GYP domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=118, Percent_Identity=39.8305084745763, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1790437, Length=115, Percent_Identity=39.1304347826087, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1790299, Length=117, Percent_Identity=39.3162393162393, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI1788905, Length=117, Percent_Identity=34.1880341880342, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1786599, Length=126, Percent_Identity=33.3333333333333, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR003607
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 45718; Mature: 45718

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEAIPPAPPRILIVDDDDSVRDVISVLLREEGYNCVVANGAEMALDLAGEEETPLVISDM
CCCCCCCCCEEEEECCCCCHHHHHHHHHHHCCCEEEEECCCCEEEEECCCCCCCEEEECC
KMPGKDGLWLLENLRERLPDTSVIMLTGYGDTESAVDCLRRGAVDYLLKPPKLTDLIRAI
CCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHCCHHHHCCCCCHHHHHHHH
ERALAKRRIEMARKRYQKKLERKVRDRTAELRSALRDIANTYQNTLLALVAALDAREHET
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
SDHSQRVVSYTSAIAQRMGIQGKELEEIGRGALLHDIGKIGVPDAVLLKPGKLTPDEWLE
CHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCHHHHHHHHCCCCCEEEECCCCCCHHHHHH
MRKHPDIGFQMIQAIPFLDTPASIVLSHQERWDGAGYPRNLQRHEIHIGARIFAVADTLD
HHCCCCHHHHHHHHCCCCCCCHHHHCCCHHHCCCCCCCCCCCHHHEEHHHHHHHHHHHHH
AMTSDRPYRKGTTFTNAIQEIKRCANTQFDPEVVRAFLDIGEEGLIRIKQEMATKKLQLP
HHHCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCC
QAEQDAHDAEAELARLTDLDDDVDAAVPGHSASDEDEPKSAVVRSVAGNKG
CCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCC
>Mature Secondary Structure
MEAIPPAPPRILIVDDDDSVRDVISVLLREEGYNCVVANGAEMALDLAGEEETPLVISDM
CCCCCCCCCEEEEECCCCCHHHHHHHHHHHCCCEEEEECCCCEEEEECCCCCCCEEEECC
KMPGKDGLWLLENLRERLPDTSVIMLTGYGDTESAVDCLRRGAVDYLLKPPKLTDLIRAI
CCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHCCHHHHCCCCCHHHHHHHH
ERALAKRRIEMARKRYQKKLERKVRDRTAELRSALRDIANTYQNTLLALVAALDAREHET
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
SDHSQRVVSYTSAIAQRMGIQGKELEEIGRGALLHDIGKIGVPDAVLLKPGKLTPDEWLE
CHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCHHHHHHHHCCCCCEEEECCCCCCHHHHHH
MRKHPDIGFQMIQAIPFLDTPASIVLSHQERWDGAGYPRNLQRHEIHIGARIFAVADTLD
HHCCCCHHHHHHHHCCCCCCCHHHHCCCHHHCCCCCCCCCCCHHHEEHHHHHHHHHHHHH
AMTSDRPYRKGTTFTNAIQEIKRCANTQFDPEVVRAFLDIGEEGLIRIKQEMATKKLQLP
HHHCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCC
QAEQDAHDAEAELARLTDLDDDVDAAVPGHSASDEDEPKSAVVRSVAGNKG
CCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11123673 [H]