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The map label for this gene is polA [H]

Identifier: 108563870

GI number: 108563870

Start: 1501256

End: 1503955

Strand: Direct

Name: polA [H]

Synonym: HPAG1_1445

Alternate gene names: 108563870

Gene position: 1501256-1503955 (Clockwise)

Preceding gene: 108563869

Following gene: 108563871

Centisome position: 94.04

GC content: 37.85

Gene sequence:

>2700_bases
ATGGAGCAGCCAGTCATTAAAGAGGGGACTTTAGCGTTAATTGATACTTTTGCGTATTTGTTTAGAAGCTATTACATGAG
CGCTAAAAATAAGCCTTTAACCAACGATAAGGGCTTTCCTACAGGGCTTTTAACGGGGCTTGTGGGCATGGTTAAAAAAT
TTTATAAAGATAAAAAAAACATGCCTTTTATCGTGTTCGCTCTAGAAAGCCAGACTAAAACTAAAAGATCTGAAAAATTG
GGCGAATACAAACAAAATCGCAAAGACGCCCCTAAAGAAATGCTTTTACAAATCCCTATCGCCTTAGAATGGTTGCAAAA
AATGGGTTTTACTTGCGTGGAGGTGAATGGGTTTGAAGCCGATGATGTGATCGCAAGCTTGGCCACGCTAAGCCCTTATA
AAACCCGCATTTATTCTAAAGATAAGGATTTTAACCAGCTTTTGAGCGATAAAATCGCGCTTTTTGATGGCAAAACGGAG
TTTTTGGCGAAAGATTGTGTGGAAAAATACGGGATTTTGCCGAGTCAATTCACGGATTATCAGGGCATTGTGGGGGATAG
TAGCGATAATTACAAGGGGGTTAAAGGCATTGGGAGCAAGAACGCTAAGGAATTGTTGCAGCGATTGGGGAGCTTGGAAA
AAATCTATGAAAATTTAGACTTGGCGAAAAATTTACTCAGCCCTAAAATGTATCAAGCCTTGATACAAGACAAAGGAAGC
GCGTTTTTAAGCAAAGAATTAGCCACTTTAGAAAGAGGATGCATTAAAGAATTTGATTTTTTAAGTTGCGCTTTTCCTAG
CGAAAACCCCTTATTGAAAATTAAAGATGAATTGAAAGAATATGGTTTTATTTCTACCTTAAGGGATTTAGAAAATTCCC
CTACGCCTTTAATTTTAGACAACGCCCCCATATTAGATAGCGTGCCTATATTAGACAACGCCTCCGCATTAGACAATGCC
CCTAAAAAATCACGCCTAATCGTTTTAGAAAACACCGAGCTTTTGAGCGCGTTTTTAGAAAAATTAAAAAATTCTAACGC
AAGGATTTTTATGCGCTTGGTGCTAAATAAAGAAAAAAAAGTTTTGGCCCTAGCGTTTTTATATGAAGATCAAGGCTATT
TTTTACCTTTAGAAGAGGCGTTATTTTCGCCCTTTTCTTTAGAGTTTTTGCAAAACGCTTTTTTTAAAATGTTACAGCAT
GCATGTATCATTGGGCATGATTTAAAGCCTTTATTAAGCTTTTTAAAAGCTAAATATCAGGTGCCTTTAGAAAACATTCG
CATCCAAGACACTCAAATTTTAGCGTTTTTAAAAAATCCGGAAAAAGTGGGGTTTGATGCAGTTTTAAAGGAATATTTGA
AAGAAGATTTAATCCCGCATGAAAAAATCAAAGATTTTAAGACAACAAGTAAGGCGGAAAAATCAGAGCAATTGAGTTTG
GAATTAAACGCTTTAAAGCGTTTGTGCGAGTATTTTGAAAAAGGGGGATTGGAAGAAAACTTGCTTTCTTTGGCCAGAGG
AGTTGAAACGCCGTTTGTGAAAGTTTTAATGGATATGGAATTTCAAGGCTTTAAGATTGATGCACCTTATTTCAAGCGAT
TAGAACAGGAGTTTAAAGATGAATTAAAAGTTTTAGAGCGCCAAATTTTGGATTTAATCGGCGTGGATTTTAACCTCAAT
TCGCCTAAGCAACTTAGCGAAGTCTTGTATGAAAAATTAGAACTTCCTCAAAATAAAAGCCATTCTACCGATGAAAAAAA
CTTGTTAAAAATCCTAGACAAGCACCCAAGTATCGCTTTGATTTTAGAATACAGGGAATTGAATAAGCTTTTTAACACTT
ACACCACCCCCTTATTGCGCCTAAAAGACAAAGACGATAAAATCCATACCACTTTTATCCAAACCGGCACAGCTACCGGG
CGTTTAAGCTCGCATTCGCCTAATTTGCAAAATATCCCGGTGCGATCGCCTAAGGGCTTACTCATTCGTAAGGGCTTTAT
TGCCAGCTCTAAAGAATATTGTTTGCTAGGGGTGGATTATTCGCAGATTGAATTGCGCTTGTTAGCCCATTTTAGCCAGG
ATAAGGATTTAATGGAGGCGTTTTTAAAGGGGCGAGACATCCATTTAGAAACTTCTAAGGCGTTGTTTGGTGGAGATTTG
GCCAAAGAAAAACGATCCATCGCTAAAAGCATTAATTTTGGGCTGGTGTATGGCATGGGGAGTAAGAAATTGAGCGAAAC
TTTAAACATCTCTTTAAATGAGGCTAAAAGCTACATAGAAGCGTATTTCAAACGATTCCCTAGCATTAAAGATTATTTAA
ACCGCATGAAAGAAGAGATTTTAAAAACTTCTAAAGCCTTTACTTTGCTTGGGCGCTATCGGGTGTTTGATTTTAATGGC
GTGAATGATTACGTTAAGGGCAATTATTTGCGAGAGGGCGTGAATGCGATTTTTCAAGGGAGTGCCAGCGATTTATTGAA
ATTAGGCATGCTCAAAGTGAGCGAGCGTTTCAAAAATAACCCTTCGGTGAGGCTGCTTTTGCAAGTGCATGACGAATTGA
TTTTTGAGATTGAAGAAAAAAATGCATTAGAGTTGCAGCAAGAAATCCAACGCATCCTCAATGATGAAGTTTATCCTTTG
AGGGTGCCGTTAGAAACGAGCGCGTTTGTAGCGAATCGTTGGAATGAACTAAAAGGTTAG

Upstream 100 bases:

>100_bases
AAGAAACACCCATTCAAAAAGACTCGTTATTTTGAAAGAGATGAGGTCAAGCTTGTGTTACAATAAATTTAAAATTTGCT
TGATTGAAGAGGGTTGAACC

Downstream 100 bases:

>100_bases
TTTTTTAATTGAGTTTAAGAAAAAATATATTATTATTTCTTTATAAGTAATACTTAGCCATTTTAAGCTAATGTAATGTG
AAGTGATTATCAAAAAATAA

Product: DNA polymerase I

Products: NA

Alternate protein names: POL I [H]

Number of amino acids: Translated: 899; Mature: 899

Protein sequence:

>899_residues
MEQPVIKEGTLALIDTFAYLFRSYYMSAKNKPLTNDKGFPTGLLTGLVGMVKKFYKDKKNMPFIVFALESQTKTKRSEKL
GEYKQNRKDAPKEMLLQIPIALEWLQKMGFTCVEVNGFEADDVIASLATLSPYKTRIYSKDKDFNQLLSDKIALFDGKTE
FLAKDCVEKYGILPSQFTDYQGIVGDSSDNYKGVKGIGSKNAKELLQRLGSLEKIYENLDLAKNLLSPKMYQALIQDKGS
AFLSKELATLERGCIKEFDFLSCAFPSENPLLKIKDELKEYGFISTLRDLENSPTPLILDNAPILDSVPILDNASALDNA
PKKSRLIVLENTELLSAFLEKLKNSNARIFMRLVLNKEKKVLALAFLYEDQGYFLPLEEALFSPFSLEFLQNAFFKMLQH
ACIIGHDLKPLLSFLKAKYQVPLENIRIQDTQILAFLKNPEKVGFDAVLKEYLKEDLIPHEKIKDFKTTSKAEKSEQLSL
ELNALKRLCEYFEKGGLEENLLSLARGVETPFVKVLMDMEFQGFKIDAPYFKRLEQEFKDELKVLERQILDLIGVDFNLN
SPKQLSEVLYEKLELPQNKSHSTDEKNLLKILDKHPSIALILEYRELNKLFNTYTTPLLRLKDKDDKIHTTFIQTGTATG
RLSSHSPNLQNIPVRSPKGLLIRKGFIASSKEYCLLGVDYSQIELRLLAHFSQDKDLMEAFLKGRDIHLETSKALFGGDL
AKEKRSIAKSINFGLVYGMGSKKLSETLNISLNEAKSYIEAYFKRFPSIKDYLNRMKEEILKTSKAFTLLGRYRVFDFNG
VNDYVKGNYLREGVNAIFQGSASDLLKLGMLKVSERFKNNPSVRLLLQVHDELIFEIEEKNALELQQEIQRILNDEVYPL
RVPLETSAFVANRWNELKG

Sequences:

>Translated_899_residues
MEQPVIKEGTLALIDTFAYLFRSYYMSAKNKPLTNDKGFPTGLLTGLVGMVKKFYKDKKNMPFIVFALESQTKTKRSEKL
GEYKQNRKDAPKEMLLQIPIALEWLQKMGFTCVEVNGFEADDVIASLATLSPYKTRIYSKDKDFNQLLSDKIALFDGKTE
FLAKDCVEKYGILPSQFTDYQGIVGDSSDNYKGVKGIGSKNAKELLQRLGSLEKIYENLDLAKNLLSPKMYQALIQDKGS
AFLSKELATLERGCIKEFDFLSCAFPSENPLLKIKDELKEYGFISTLRDLENSPTPLILDNAPILDSVPILDNASALDNA
PKKSRLIVLENTELLSAFLEKLKNSNARIFMRLVLNKEKKVLALAFLYEDQGYFLPLEEALFSPFSLEFLQNAFFKMLQH
ACIIGHDLKPLLSFLKAKYQVPLENIRIQDTQILAFLKNPEKVGFDAVLKEYLKEDLIPHEKIKDFKTTSKAEKSEQLSL
ELNALKRLCEYFEKGGLEENLLSLARGVETPFVKVLMDMEFQGFKIDAPYFKRLEQEFKDELKVLERQILDLIGVDFNLN
SPKQLSEVLYEKLELPQNKSHSTDEKNLLKILDKHPSIALILEYRELNKLFNTYTTPLLRLKDKDDKIHTTFIQTGTATG
RLSSHSPNLQNIPVRSPKGLLIRKGFIASSKEYCLLGVDYSQIELRLLAHFSQDKDLMEAFLKGRDIHLETSKALFGGDL
AKEKRSIAKSINFGLVYGMGSKKLSETLNISLNEAKSYIEAYFKRFPSIKDYLNRMKEEILKTSKAFTLLGRYRVFDFNG
VNDYVKGNYLREGVNAIFQGSASDLLKLGMLKVSERFKNNPSVRLLLQVHDELIFEIEEKNALELQQEIQRILNDEVYPL
RVPLETSAFVANRWNELKG
>Mature_899_residues
MEQPVIKEGTLALIDTFAYLFRSYYMSAKNKPLTNDKGFPTGLLTGLVGMVKKFYKDKKNMPFIVFALESQTKTKRSEKL
GEYKQNRKDAPKEMLLQIPIALEWLQKMGFTCVEVNGFEADDVIASLATLSPYKTRIYSKDKDFNQLLSDKIALFDGKTE
FLAKDCVEKYGILPSQFTDYQGIVGDSSDNYKGVKGIGSKNAKELLQRLGSLEKIYENLDLAKNLLSPKMYQALIQDKGS
AFLSKELATLERGCIKEFDFLSCAFPSENPLLKIKDELKEYGFISTLRDLENSPTPLILDNAPILDSVPILDNASALDNA
PKKSRLIVLENTELLSAFLEKLKNSNARIFMRLVLNKEKKVLALAFLYEDQGYFLPLEEALFSPFSLEFLQNAFFKMLQH
ACIIGHDLKPLLSFLKAKYQVPLENIRIQDTQILAFLKNPEKVGFDAVLKEYLKEDLIPHEKIKDFKTTSKAEKSEQLSL
ELNALKRLCEYFEKGGLEENLLSLARGVETPFVKVLMDMEFQGFKIDAPYFKRLEQEFKDELKVLERQILDLIGVDFNLN
SPKQLSEVLYEKLELPQNKSHSTDEKNLLKILDKHPSIALILEYRELNKLFNTYTTPLLRLKDKDDKIHTTFIQTGTATG
RLSSHSPNLQNIPVRSPKGLLIRKGFIASSKEYCLLGVDYSQIELRLLAHFSQDKDLMEAFLKGRDIHLETSKALFGGDL
AKEKRSIAKSINFGLVYGMGSKKLSETLNISLNEAKSYIEAYFKRFPSIKDYLNRMKEEILKTSKAFTLLGRYRVFDFNG
VNDYVKGNYLREGVNAIFQGSASDLLKLGMLKVSERFKNNPSVRLLLQVHDELIFEIEEKNALELQQEIQRILNDEVYPL
RVPLETSAFVANRWNELKG

Specific function: In addition to polymerase activity, this DNA polymerase exhibits 3' to 5' and 5' to 3' exonuclease activity [H]

COG id: COG0749

COG function: function code L; DNA polymerase I - 3'-5' exonuclease and polymerase domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 5'-3' exonuclease domain [H]

Homologues:

Organism=Homo sapiens, GI112420958, Length=515, Percent_Identity=28.7378640776699, Blast_Score=185, Evalue=2e-46,
Organism=Homo sapiens, GI139394648, Length=542, Percent_Identity=25.6457564575646, Blast_Score=150, Evalue=4e-36,
Organism=Escherichia coli, GI1790294, Length=949, Percent_Identity=31.7175974710221, Blast_Score=378, Evalue=1e-105,
Organism=Escherichia coli, GI226510970, Length=192, Percent_Identity=35.4166666666667, Blast_Score=103, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI86563272, Length=402, Percent_Identity=29.8507462686567, Blast_Score=186, Evalue=4e-47,
Organism=Drosophila melanogaster, GI17933644, Length=539, Percent_Identity=28.7569573283859, Blast_Score=181, Evalue=3e-45,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002562
- InterPro:   IPR020046
- InterPro:   IPR020045
- InterPro:   IPR002421
- InterPro:   IPR020047
- InterPro:   IPR019760
- InterPro:   IPR001098
- InterPro:   IPR018320
- InterPro:   IPR002298
- InterPro:   IPR008918
- InterPro:   IPR012337 [H]

Pfam domain/function: PF01367 5_3_exonuc; PF02739 5_3_exonuc_N; PF00476 DNA_pol_A [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 102714; Mature: 102714

Theoretical pI: Translated: 8.49; Mature: 8.49

Prosite motif: PS00447 DNA_POLYMERASE_A

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEQPVIKEGTLALIDTFAYLFRSYYMSAKNKPLTNDKGFPTGLLTGLVGMVKKFYKDKKN
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCC
MPFIVFALESQTKTKRSEKLGEYKQNRKDAPKEMLLQIPIALEWLQKMGFTCVEVNGFEA
CCEEEEEECCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCH
DDVIASLATLSPYKTRIYSKDKDFNQLLSDKIALFDGKTEFLAKDCVEKYGILPSQFTDY
HHHHHHHHHCCHHHHHHCCCCCHHHHHHHHHHEEECCCHHHHHHHHHHHHCCCCCCCCCC
QGIVGDSSDNYKGVKGIGSKNAKELLQRLGSLEKIYENLDLAKNLLSPKMYQALIQDKGS
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCH
AFLSKELATLERGCIKEFDFLSCAFPSENPLLKIKDELKEYGFISTLRDLENSPTPLILD
HHHHHHHHHHHHHHHHHCCCCEECCCCCCCEEEEHHHHHHHHHHHHHHHHCCCCCCEEEC
NAPILDSVPILDNASALDNAPKKSRLIVLENTELLSAFLEKLKNSNARIFMRLVLNKEKK
CCCCCCCCCCCCCCHHHCCCCCCCEEEEEECHHHHHHHHHHHHCCCCEEEEEEHHCCCCC
VLALAFLYEDQGYFLPLEEALFSPFSLEFLQNAFFKMLQHACIIGHDLKPLLSFLKAKYQ
EEEEEEEEECCCCEEEHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHC
VPLENIRIQDTQILAFLKNPEKVGFDAVLKEYLKEDLIPHEKIKDFKTTSKAEKSEQLSL
CCHHHEEECHHHEEEEECCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
ELNALKRLCEYFEKGGLEENLLSLARGVETPFVKVLMDMEFQGFKIDAPYFKRLEQEFKD
HHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHCCCCCCEEECCHHHHHHHHHHHH
ELKVLERQILDLIGVDFNLNSPKQLSEVLYEKLELPQNKSHSTDEKNLLKILDKHPSIAL
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCEEE
ILEYRELNKLFNTYTTPLLRLKDKDDKIHTTFIQTGTATGRLSSHSPNLQNIPVRSPKGL
EEEHHHHHHHHHHHCCCCEEEECCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCC
LIRKGFIASSKEYCLLGVDYSQIELRLLAHFSQDKDLMEAFLKGRDIHLETSKALFGGDL
EEECCCCCCCCCEEEEECCHHHHHHHHHHHHCCCHHHHHHHHCCCCEEEECCHHHHCCHH
AKEKRSIAKSINFGLVYGMGSKKLSETLNISLNEAKSYIEAYFKRFPSIKDYLNRMKEEI
HHHHHHHHHHCCCEEEEECCCHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
LKTSKAFTLLGRYRVFDFNGVNDYVKGNYLREGVNAIFQGSASDLLKLGMLKVSERFKNN
HHHHHHHHHHHCEEEEEECCCCHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCC
PSVRLLLQVHDELIFEIEEKNALELQQEIQRILNDEVYPLRVPLETSAFVANRWNELKG
CCEEEHHHHHHHHHEEECCCHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MEQPVIKEGTLALIDTFAYLFRSYYMSAKNKPLTNDKGFPTGLLTGLVGMVKKFYKDKKN
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCC
MPFIVFALESQTKTKRSEKLGEYKQNRKDAPKEMLLQIPIALEWLQKMGFTCVEVNGFEA
CCEEEEEECCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCH
DDVIASLATLSPYKTRIYSKDKDFNQLLSDKIALFDGKTEFLAKDCVEKYGILPSQFTDY
HHHHHHHHHCCHHHHHHCCCCCHHHHHHHHHHEEECCCHHHHHHHHHHHHCCCCCCCCCC
QGIVGDSSDNYKGVKGIGSKNAKELLQRLGSLEKIYENLDLAKNLLSPKMYQALIQDKGS
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCH
AFLSKELATLERGCIKEFDFLSCAFPSENPLLKIKDELKEYGFISTLRDLENSPTPLILD
HHHHHHHHHHHHHHHHHCCCCEECCCCCCCEEEEHHHHHHHHHHHHHHHHCCCCCCEEEC
NAPILDSVPILDNASALDNAPKKSRLIVLENTELLSAFLEKLKNSNARIFMRLVLNKEKK
CCCCCCCCCCCCCCHHHCCCCCCCEEEEEECHHHHHHHHHHHHCCCCEEEEEEHHCCCCC
VLALAFLYEDQGYFLPLEEALFSPFSLEFLQNAFFKMLQHACIIGHDLKPLLSFLKAKYQ
EEEEEEEEECCCCEEEHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHC
VPLENIRIQDTQILAFLKNPEKVGFDAVLKEYLKEDLIPHEKIKDFKTTSKAEKSEQLSL
CCHHHEEECHHHEEEEECCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
ELNALKRLCEYFEKGGLEENLLSLARGVETPFVKVLMDMEFQGFKIDAPYFKRLEQEFKD
HHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHCCCCCCEEECCHHHHHHHHHHHH
ELKVLERQILDLIGVDFNLNSPKQLSEVLYEKLELPQNKSHSTDEKNLLKILDKHPSIAL
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCEEE
ILEYRELNKLFNTYTTPLLRLKDKDDKIHTTFIQTGTATGRLSSHSPNLQNIPVRSPKGL
EEEHHHHHHHHHHHCCCCEEEECCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCC
LIRKGFIASSKEYCLLGVDYSQIELRLLAHFSQDKDLMEAFLKGRDIHLETSKALFGGDL
EEECCCCCCCCCEEEEECCHHHHHHHHHHHHCCCHHHHHHHHCCCCEEEECCHHHHCCHH
AKEKRSIAKSINFGLVYGMGSKKLSETLNISLNEAKSYIEAYFKRFPSIKDYLNRMKEEI
HHHHHHHHHHCCCEEEEECCCHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
LKTSKAFTLLGRYRVFDFNGVNDYVKGNYLREGVNAIFQGSASDLLKLGMLKVSERFKNN
HHHHHHHHHHHCEEEEEECCCCHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCC
PSVRLLLQVHDELIFEIEEKNALELQQEIQRILNDEVYPLRVPLETSAFVANRWNELKG
CCEEEHHHHHHHHHEEECCCHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]