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The map label for this gene is ispE

Identifier: 108563794

GI number: 108563794

Start: 1421564

End: 1422388

Strand: Direct

Name: ispE

Synonym: HPAG1_1369

Alternate gene names: 108563794

Gene position: 1421564-1422388 (Clockwise)

Preceding gene: 108563793

Following gene: 108563795

Centisome position: 89.05

GC content: 38.42

Gene sequence:

>825_bases
TTGATGCATGTCTTTGAAGTTTATCCTAAAGTCAATATTTTTTTAAAAATCCTTCACAAAGAAGGGGCTTACCACAAGCT
TATTTCTCGCATGTGTTTAGTCAAAGATAAGCTAAAGGATATTATTAGCGTCAAAAGTGCGCTTTCCTTTTCACTAAAAG
GGGATTTTGACTGCCCTTTAGAAGAAAACTCGCTCTTTAAAGCCCTCCAAATTTTAAAGAATTTTTTAAAATCAAAAAAT
TTTTCTCATTCTGCCATCAAATCCCTAGACACCCTAGCGATTGAAGTGGAAAAAAATATCCCCACTCAAGCCGGATTAGG
CGGTGGGAGCGCTGATGCTGGGGGGCTATTGTATCATTTGAATCACATTTTTGACTGGCGTTTGAGTTTAGAAGAGCTTT
ATAGCATGGGATCTTTAGTGGGTGCGGACACCAATTTTTTCATCTCGCAACACAAAAGCGCTAACGCTACTTCTTATGGC
GAAGTCATTGAAAATTTTGAAGAAGAGCCTTTAGAAAATCGCCTAGAAATCTATGCACCAAATCATGTTTTTTGCAGCAC
TAAAGCCATCTATCAAGCCTATAAACCTGAAACTTGTTTTTCTCAAACTAAAGAATGGCTTAAAAAGCCGAGTTTGGAAT
GCCTAAAAACTTGCGACAGAAGCGAATTAAACGATCTTTTAAAGCCGGCTTTACTCACTAACCAAGCCTTAAGAGATATA
GAAAGCGAATTAGGCAAGGAGTGGTTTTTTAGCGGGAGCGGGAGCGCGTTTTTTAGGCTAAAAAATACTCAAAAGGGAGC
GAATGAAACTCATTGCCAGCAATAA

Upstream 100 bases:

>100_bases
TGCGCGCTGAACTCAAAGAGGCCATTGTGAGCGAAAACCAAAAAGCTTCTGCGTGCGTAGATGAAAGCGTGTTAGAAAAC
ATCAAAAAGGTCATTAAGCC

Downstream 100 bases:

>100_bases
AAAAGCCTATTTTGACTATGAAATTTTGGAAACTTTAGAAGCGGGATTAGCGCTTTTAGGTTCTGAAGTGAAGGCTTTGA
GGCAAACTAGGGTGAATTTA

Product: 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase

Products: NA

Alternate protein names: CMK; 4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Number of amino acids: Translated: 274; Mature: 274

Protein sequence:

>274_residues
MMHVFEVYPKVNIFLKILHKEGAYHKLISRMCLVKDKLKDIISVKSALSFSLKGDFDCPLEENSLFKALQILKNFLKSKN
FSHSAIKSLDTLAIEVEKNIPTQAGLGGGSADAGGLLYHLNHIFDWRLSLEELYSMGSLVGADTNFFISQHKSANATSYG
EVIENFEEEPLENRLEIYAPNHVFCSTKAIYQAYKPETCFSQTKEWLKKPSLECLKTCDRSELNDLLKPALLTNQALRDI
ESELGKEWFFSGSGSAFFRLKNTQKGANETHCQQ

Sequences:

>Translated_274_residues
MMHVFEVYPKVNIFLKILHKEGAYHKLISRMCLVKDKLKDIISVKSALSFSLKGDFDCPLEENSLFKALQILKNFLKSKN
FSHSAIKSLDTLAIEVEKNIPTQAGLGGGSADAGGLLYHLNHIFDWRLSLEELYSMGSLVGADTNFFISQHKSANATSYG
EVIENFEEEPLENRLEIYAPNHVFCSTKAIYQAYKPETCFSQTKEWLKKPSLECLKTCDRSELNDLLKPALLTNQALRDI
ESELGKEWFFSGSGSAFFRLKNTQKGANETHCQQ
>Mature_274_residues
MMHVFEVYPKVNIFLKILHKEGAYHKLISRMCLVKDKLKDIISVKSALSFSLKGDFDCPLEENSLFKALQILKNFLKSKN
FSHSAIKSLDTLAIEVEKNIPTQAGLGGGSADAGGLLYHLNHIFDWRLSLEELYSMGSLVGADTNFFISQHKSANATSYG
EVIENFEEEPLENRLEIYAPNHVFCSTKAIYQAYKPETCFSQTKEWLKKPSLECLKTCDRSELNDLLKPALLTNQALRDI
ESELGKEWFFSGSGSAFFRLKNTQKGANETHCQQ

Specific function: Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol

COG id: COG1947

COG function: function code I; 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. IspE subfamily

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPE_HELPH (Q1CRI6)

Other databases:

- EMBL:   CP000241
- RefSeq:   YP_628110.1
- ProteinModelPortal:   Q1CRI6
- SMR:   Q1CRI6
- STRING:   Q1CRI6
- GeneID:   4098040
- GenomeReviews:   CP000241_GR
- KEGG:   hpa:HPAG1_1369
- eggNOG:   COG1947
- HOGENOM:   HBG734593
- OMA:   ECLKTCD
- ProtClustDB:   PRK04181
- BioCyc:   HPYL357544:HPAG1_1369-MONOMER
- HAMAP:   MF_00061
- InterPro:   IPR006204
- InterPro:   IPR004424
- InterPro:   IPR020568
- InterPro:   IPR014721
- Gene3D:   G3DSA:3.30.230.10
- PANTHER:   PTHR20861:SF2
- PIRSF:   PIRSF010376
- TIGRFAMs:   TIGR00154

Pfam domain/function: PF00288 GHMP_kinases_N; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.1.148

Molecular weight: Translated: 30987; Mature: 30987

Theoretical pI: Translated: 6.99; Mature: 6.99

Prosite motif: NA

Important sites: ACT_SITE 10-10 ACT_SITE 143-143

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMHVFEVYPKVNIFLKILHKEGAYHKLISRMCLVKDKLKDIISVKSALSFSLKGDFDCPL
CCCHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
EENSLFKALQILKNFLKSKNFSHSAIKSLDTLAIEVEKNIPTQAGLGGGSADAGGLLYHL
CCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHEEEECCCCCCCCCCCCCCCCCCHHHHHH
NHIFDWRLSLEELYSMGSLVGADTNFFISQHKSANATSYGEVIENFEEEPLENRLEIYAP
HHHHHHHCCHHHHHHHHHHHCCCCHHHHCCCCCCCCCHHHHHHHHHHHCHHHCCEEEECC
NHVFCSTKAIYQAYKPETCFSQTKEWLKKPSLECLKTCDRSELNDLLKPALLTNQALRDI
CCEEEHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
ESELGKEWFFSGSGSAFFRLKNTQKGANETHCQQ
HHHHCHHHEECCCCCEEEEECCCCCCCCCCCCCC
>Mature Secondary Structure
MMHVFEVYPKVNIFLKILHKEGAYHKLISRMCLVKDKLKDIISVKSALSFSLKGDFDCPL
CCCHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
EENSLFKALQILKNFLKSKNFSHSAIKSLDTLAIEVEKNIPTQAGLGGGSADAGGLLYHL
CCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHEEEECCCCCCCCCCCCCCCCCCHHHHHH
NHIFDWRLSLEELYSMGSLVGADTNFFISQHKSANATSYGEVIENFEEEPLENRLEIYAP
HHHHHHHCCHHHHHHHHHHHCCCCHHHHCCCCCCCCCHHHHHHHHHHHCHHHCCEEEECC
NHVFCSTKAIYQAYKPETCFSQTKEWLKKPSLECLKTCDRSELNDLLKPALLTNQALRDI
CCEEEHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
ESELGKEWFFSGSGSAFFRLKNTQKGANETHCQQ
HHHHCHHHEECCCCCEEEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA