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The map label for this gene is purU [H]

Identifier: 108563785

GI number: 108563785

Start: 1412719

End: 1413600

Strand: Reverse

Name: purU [H]

Synonym: HPAG1_1360

Alternate gene names: 108563785

Gene position: 1413600-1412719 (Counterclockwise)

Preceding gene: 108563786

Following gene: 108563784

Centisome position: 88.55

GC content: 38.89

Gene sequence:

>882_bases
ATGTTAGAATTTATTTTAAAAATTCAAGCTAGAGACTCTAAAGGCTTGGTGAGCGCGATTAGCACCACTATCGCTAACAA
GGGCTATAACATCGTCAAAAACGATGAATTTGTTGATCCCTTAAAACAACGCTTTTTCATGCGCTTAAAAATCCAAAAAG
AAATTAAGCCCTTGAATGTTGAAATGAAAGAGCAAGAAGAGCGATCCTTAAAAACCGCTCTTTTTAAAGCCCTAGAAAAC
TTTAACGAACTATTGATTGAAGTCATTTTAACGCATAAAAAAAACATCATTCTGCTCGCTACTAAAGAGAGCCATTGCTT
AGGGGATTTGCTTTTAAGGGTGTATGGAGGGGAATTGAACGCTCAAATTTTAGGCGTTATTTCCAACCACGAGATTTTAC
GCCCTTTAGTGGAAAAATTTGACATCCCTTATTTTTATGCGCCTTGCGATAATCAAGTTTTGCATGAAAAAGAAGTTTTA
GAAATCATTAAAAATCTGGAATTAAAACACAAAGTGAGCACGGATTTGCTCGTTTTAGCCAAATACATGCGCATTTTAAG
CCATGATTTTACGAAGCGCTATGAAAATCAGATCTTAAATATCCATCATAGTTTCTTGCCCGCATTCATCGGGGCTAACC
CTTACCAGCAAGCGTTTGAAAGGGGCGTGAAAGTCATCGGGGCCACGGCGCATTTTGTGAATGAAAGCCTTGATGCCGGA
CCGATTATCATACAAGACACTCTGCCCATTAACCACAACTATAGCGTGGAAAAAATGCGCCTAGCGGGCAAGGATATAGA
AAAACTGGTTTTGGCTAGGGCTTTAAAACTCGTTTTAGAAGATCGGGTGTTTGTGCATGAAAACAAAACGGTGGTGTTTT
GA

Upstream 100 bases:

>100_bases
TGGAAAAAAGCCCTATGGAGCGCTTCATTGAAAAAGCCACGCAATCAGCGAGTAATATCATCACGCAAGCCTTTGGCTAT
CAATTGTTAATGAGATGAAG

Downstream 100 bases:

>100_bases
ATGCTTTTAGATTGCAGCAATCTCAATGAAGAACCCCTAAAATCTAGCGTAGCCAAAGCCTTTTTTGAAAACTTTGACTT
TAGCGGAGATAAAATTGATT

Product: formyltetrahydrofolate hydrolase

Products: NA

Alternate protein names: Formyl-FH(4) hydrolase [H]

Number of amino acids: Translated: 293; Mature: 293

Protein sequence:

>293_residues
MLEFILKIQARDSKGLVSAISTTIANKGYNIVKNDEFVDPLKQRFFMRLKIQKEIKPLNVEMKEQEERSLKTALFKALEN
FNELLIEVILTHKKNIILLATKESHCLGDLLLRVYGGELNAQILGVISNHEILRPLVEKFDIPYFYAPCDNQVLHEKEVL
EIIKNLELKHKVSTDLLVLAKYMRILSHDFTKRYENQILNIHHSFLPAFIGANPYQQAFERGVKVIGATAHFVNESLDAG
PIIIQDTLPINHNYSVEKMRLAGKDIEKLVLARALKLVLEDRVFVHENKTVVF

Sequences:

>Translated_293_residues
MLEFILKIQARDSKGLVSAISTTIANKGYNIVKNDEFVDPLKQRFFMRLKIQKEIKPLNVEMKEQEERSLKTALFKALEN
FNELLIEVILTHKKNIILLATKESHCLGDLLLRVYGGELNAQILGVISNHEILRPLVEKFDIPYFYAPCDNQVLHEKEVL
EIIKNLELKHKVSTDLLVLAKYMRILSHDFTKRYENQILNIHHSFLPAFIGANPYQQAFERGVKVIGATAHFVNESLDAG
PIIIQDTLPINHNYSVEKMRLAGKDIEKLVLARALKLVLEDRVFVHENKTVVF
>Mature_293_residues
MLEFILKIQARDSKGLVSAISTTIANKGYNIVKNDEFVDPLKQRFFMRLKIQKEIKPLNVEMKEQEERSLKTALFKALEN
FNELLIEVILTHKKNIILLATKESHCLGDLLLRVYGGELNAQILGVISNHEILRPLVEKFDIPYFYAPCDNQVLHEKEVL
EIIKNLELKHKVSTDLLVLAKYMRILSHDFTKRYENQILNIHHSFLPAFIGANPYQQAFERGVKVIGATAHFVNESLDAG
PIIIQDTLPINHNYSVEKMRLAGKDIEKLVLARALKLVLEDRVFVHENKTVVF

Specific function: Produces formate from formyl-tetrahydrofolate. Provides the major source of formate for the purT-dependent synthesis of 5'-phosphoribosyl-N-formylglycinamide (FGAR) during aerobic growth. Has a role in regulating the one-carbon pool [H]

COG id: COG0788

COG function: function code F; Formyltetrahydrofolate hydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=177, Percent_Identity=36.7231638418079, Blast_Score=99, Evalue=7e-21,
Organism=Homo sapiens, GI209869995, Length=177, Percent_Identity=36.7231638418079, Blast_Score=99, Evalue=7e-21,
Organism=Homo sapiens, GI209869993, Length=177, Percent_Identity=36.7231638418079, Blast_Score=99, Evalue=7e-21,
Organism=Escherichia coli, GI1787483, Length=297, Percent_Identity=42.7609427609428, Blast_Score=239, Evalue=2e-64,
Organism=Escherichia coli, GI1788846, Length=127, Percent_Identity=33.8582677165354, Blast_Score=80, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17567511, Length=144, Percent_Identity=35.4166666666667, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24582400, Length=125, Percent_Identity=32.8, Blast_Score=77, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR002376
- InterPro:   IPR004810 [H]

Pfam domain/function: PF01842 ACT; PF00551 Formyl_trans_N [H]

EC number: =3.5.1.10 [H]

Molecular weight: Translated: 33694; Mature: 33694

Theoretical pI: Translated: 8.62; Mature: 8.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEFILKIQARDSKGLVSAISTTIANKGYNIVKNDEFVDPLKQRFFMRLKIQKEIKPLNV
CCEEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCC
EMKEQEERSLKTALFKALENFNELLIEVILTHKKNIILLATKESHCLGDLLLRVYGGELN
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHCCCCC
AQILGVISNHEILRPLVEKFDIPYFYAPCDNQVLHEKEVLEIIKNLELKHKVSTDLLVLA
EEEEEEECCHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHH
KYMRILSHDFTKRYENQILNIHHSFLPAFIGANPYQQAFERGVKVIGATAHFVNESLDAG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
PIIIQDTLPINHNYSVEKMRLAGKDIEKLVLARALKLVLEDRVFVHENKTVVF
CEEEEECCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHCCEEEECCCEEEC
>Mature Secondary Structure
MLEFILKIQARDSKGLVSAISTTIANKGYNIVKNDEFVDPLKQRFFMRLKIQKEIKPLNV
CCEEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCC
EMKEQEERSLKTALFKALENFNELLIEVILTHKKNIILLATKESHCLGDLLLRVYGGELN
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHCCCCC
AQILGVISNHEILRPLVEKFDIPYFYAPCDNQVLHEKEVLEIIKNLELKHKVSTDLLVLA
EEEEEEECCHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHH
KYMRILSHDFTKRYENQILNIHHSFLPAFIGANPYQQAFERGVKVIGATAHFVNESLDAG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
PIIIQDTLPINHNYSVEKMRLAGKDIEKLVLARALKLVLEDRVFVHENKTVVF
CEEEEECCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHCCEEEECCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 8335255 [H]