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The map label for this gene is dnaB [H]

Identifier: 108563734

GI number: 108563734

Start: 1355049

End: 1356515

Strand: Reverse

Name: dnaB [H]

Synonym: HPAG1_1309

Alternate gene names: 108563734

Gene position: 1356515-1355049 (Counterclockwise)

Preceding gene: 108563735

Following gene: 108563733

Centisome position: 84.98

GC content: 41.45

Gene sequence:

>1467_bases
ATGGATCATTTAAAGCATTTGCAGCAATTGCAAAACATTGAAAGGATCGTGCTTTCAGGCATTGTGTTGGCCAATCATAA
GATTGAAGAGGTCCATAGCGTTTTAGAGCCTAGCGACTTTTACTACCCGCCTAACGGCTTGTTTTTTGAAATCGCTTTAA
AACTGCATGAAGAAGATTGCCCCATTGATGAGAATTTTATCCGCCAAAAAATGCCTAAAGACAAGCAGATCAAAGAAGAG
GATCTGGTCGCTATTTTTGCGGCAAGCCCTATAGATAATATTGAAGCCTATGTGGAAGAGATTAAAAACGCTTCCATTAA
ACGAAAACTTTTTGGCTTGGCTAACACCATTAGAGAGCAAGCCCTAGAAAGCGCACAAAAATCCAGCGATATTTTAGGCG
CTGTGGAGCGAGAAGTCTATGCGTTATTGAATGGTAGCACGATTGAGGGCTTTAGGAGCATTAAAGAAGTGCTTGAAAGC
ACGATGGATCTCATTACAGAAAACCAAAGAAAAGGGAGTTTGGAAGTTACTGGCATACCGACTGGCTTTGTCCAGTTGGA
TAATTATACGAGCGGTTTTAATAAGGGGAGTTTAATCATTATAGGGGCAAGGCCGTCTATGGGTAAAACCAGTTTGATGA
TGAACATGGTCTTAAGCGCGCTCAATGACGATAGGGGGGTAGCGGTTTTTAGTTTGGAAATGTCCGCAGAGCAACTCGCT
TTAAGGGCGTTATCGGATCTCACTTCCATTAACATGCATGATTTAGAAAGCGGGAGGCTTGATGATGATCAATGGGAAAA
TTTAGCCAAATGCTACGATCACCTTTCTTGCAAAAAACTCTTTTTCTACGATAAAAGCTATGTGAGGATAGAGCAAATCC
GCTTGCAACTGCGAAAGCTTAAATCCCAACACAAGGAATTGGGTATCGCTTTTATTGACTATTTGCAACTCATGTCAGGG
AGTAAGGCCACTAAAGAGCGCCATGAGCAAATCGCTGAAATTTCAAGGGAGCTTAAGACTTTAGCCAGAGAGTTAGAAAT
CCCTATCATAGCGTTAGTGCAACTCAACCGCAGCCTAGAAAACCGAGACGATAAACGGCCCATTCTTTCGGATATTAAAG
ACAGCGGGGGGATTGAACAAGACGCTGATATTGTTTTATTTTTATACAGAGGCTATATCTATCAAATGAGGGCTGAAGAC
AACAAAATAGACAAACTCAAAAAAGAAGGCAAAATTGAAGAGGCGCAAGAGTTGCACCTAAAAGTTAATGAAGAAAGGCG
TATCCACAAGCAAAATGGCAGCATTGAAGAGGCTGAAATCATCGTGGCTAAAAACAGGAATGGGGCTACAGGAACGGTTT
ATACGCGCTTTAACGCTCCTTTCACGCGCTATGAAGACATGCCCATAGATTCCCATTTAGAAGAAAACCAAGAAACTAAA
GTGGATTATGATATAGTTACAACTTGA

Upstream 100 bases:

>100_bases
AGTTTAGCGCACGCCCTAGCGAGTTTAGAATTGAAGAACAATTACGCTTTAACGCCCCTAGATTTGATAGAAAAGATCAA
ACGATTATAAAAGGATAAAA

Downstream 100 bases:

>100_bases
AAGACAAAACTTTTCAGGGGGCGTTTGAACTTCTTACAACCCCCAAAGAATACTTATGGTGTGGGGTGTTTTTAAGCCTT
TTGTTGGCAATCAATCTTTA

Product: replicative DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 488; Mature: 488

Protein sequence:

>488_residues
MDHLKHLQQLQNIERIVLSGIVLANHKIEEVHSVLEPSDFYYPPNGLFFEIALKLHEEDCPIDENFIRQKMPKDKQIKEE
DLVAIFAASPIDNIEAYVEEIKNASIKRKLFGLANTIREQALESAQKSSDILGAVEREVYALLNGSTIEGFRSIKEVLES
TMDLITENQRKGSLEVTGIPTGFVQLDNYTSGFNKGSLIIIGARPSMGKTSLMMNMVLSALNDDRGVAVFSLEMSAEQLA
LRALSDLTSINMHDLESGRLDDDQWENLAKCYDHLSCKKLFFYDKSYVRIEQIRLQLRKLKSQHKELGIAFIDYLQLMSG
SKATKERHEQIAEISRELKTLARELEIPIIALVQLNRSLENRDDKRPILSDIKDSGGIEQDADIVLFLYRGYIYQMRAED
NKIDKLKKEGKIEEAQELHLKVNEERRIHKQNGSIEEAEIIVAKNRNGATGTVYTRFNAPFTRYEDMPIDSHLEENQETK
VDYDIVTT

Sequences:

>Translated_488_residues
MDHLKHLQQLQNIERIVLSGIVLANHKIEEVHSVLEPSDFYYPPNGLFFEIALKLHEEDCPIDENFIRQKMPKDKQIKEE
DLVAIFAASPIDNIEAYVEEIKNASIKRKLFGLANTIREQALESAQKSSDILGAVEREVYALLNGSTIEGFRSIKEVLES
TMDLITENQRKGSLEVTGIPTGFVQLDNYTSGFNKGSLIIIGARPSMGKTSLMMNMVLSALNDDRGVAVFSLEMSAEQLA
LRALSDLTSINMHDLESGRLDDDQWENLAKCYDHLSCKKLFFYDKSYVRIEQIRLQLRKLKSQHKELGIAFIDYLQLMSG
SKATKERHEQIAEISRELKTLARELEIPIIALVQLNRSLENRDDKRPILSDIKDSGGIEQDADIVLFLYRGYIYQMRAED
NKIDKLKKEGKIEEAQELHLKVNEERRIHKQNGSIEEAEIIVAKNRNGATGTVYTRFNAPFTRYEDMPIDSHLEENQETK
VDYDIVTT
>Mature_488_residues
MDHLKHLQQLQNIERIVLSGIVLANHKIEEVHSVLEPSDFYYPPNGLFFEIALKLHEEDCPIDENFIRQKMPKDKQIKEE
DLVAIFAASPIDNIEAYVEEIKNASIKRKLFGLANTIREQALESAQKSSDILGAVEREVYALLNGSTIEGFRSIKEVLES
TMDLITENQRKGSLEVTGIPTGFVQLDNYTSGFNKGSLIIIGARPSMGKTSLMMNMVLSALNDDRGVAVFSLEMSAEQLA
LRALSDLTSINMHDLESGRLDDDQWENLAKCYDHLSCKKLFFYDKSYVRIEQIRLQLRKLKSQHKELGIAFIDYLQLMSG
SKATKERHEQIAEISRELKTLARELEIPIIALVQLNRSLENRDDKRPILSDIKDSGGIEQDADIVLFLYRGYIYQMRAED
NKIDKLKKEGKIEEAQELHLKVNEERRIHKQNGSIEEAEIIVAKNRNGATGTVYTRFNAPFTRYEDMPIDSHLEENQETK
VDYDIVTT

Specific function: Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with dnaC protein, primase, and other prepriming proteins [

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=466, Percent_Identity=33.2618025751073, Blast_Score=254, Evalue=1e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 55766; Mature: 55766

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDHLKHLQQLQNIERIVLSGIVLANHKIEEVHSVLEPSDFYYPPNGLFFEIALKLHEEDC
CCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCEECCCCCEEEEEEEECCCCC
PIDENFIRQKMPKDKQIKEEDLVAIFAASPIDNIEAYVEEIKNASIKRKLFGLANTIREQ
CCCHHHHHHHCCCCCCCCHHCEEEEEECCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
ALESAQKSSDILGAVEREVYALLNGSTIEGFRSIKEVLESTMDLITENQRKGSLEVTGIP
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECC
TGFVQLDNYTSGFNKGSLIIIGARPSMGKTSLMMNMVLSALNDDRGVAVFSLEMSAEQLA
CCEEEECCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHH
LRALSDLTSINMHDLESGRLDDDQWENLAKCYDHLSCKKLFFYDKSYVRIEQIRLQLRKL
HHHHHHHHHCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHH
KSQHKELGIAFIDYLQLMSGSKATKERHEQIAEISRELKTLARELEIPIIALVQLNRSLE
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
NRDDKRPILSDIKDSGGIEQDADIVLFLYRGYIYQMRAEDNKIDKLKKEGKIEEAQELHL
CCCCCCHHHHHHHHCCCCCCCCCEEEEEECCCEEEEECCCCHHHHHHHCCCHHHHHHHHE
KVNEERRIHKQNGSIEEAEIIVAKNRNGATGTVYTRFNAPFTRYEDMPIDSHLEENQETK
EECHHHHHHHCCCCCCEEEEEEEECCCCCCEEEEEEECCCCCCCCCCCCHHHHCCCCCCC
VDYDIVTT
CCEEEECC
>Mature Secondary Structure
MDHLKHLQQLQNIERIVLSGIVLANHKIEEVHSVLEPSDFYYPPNGLFFEIALKLHEEDC
CCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCEECCCCCEEEEEEEECCCCC
PIDENFIRQKMPKDKQIKEEDLVAIFAASPIDNIEAYVEEIKNASIKRKLFGLANTIREQ
CCCHHHHHHHCCCCCCCCHHCEEEEEECCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
ALESAQKSSDILGAVEREVYALLNGSTIEGFRSIKEVLESTMDLITENQRKGSLEVTGIP
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECC
TGFVQLDNYTSGFNKGSLIIIGARPSMGKTSLMMNMVLSALNDDRGVAVFSLEMSAEQLA
CCEEEECCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHH
LRALSDLTSINMHDLESGRLDDDQWENLAKCYDHLSCKKLFFYDKSYVRIEQIRLQLRKL
HHHHHHHHHCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHH
KSQHKELGIAFIDYLQLMSGSKATKERHEQIAEISRELKTLARELEIPIIALVQLNRSLE
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
NRDDKRPILSDIKDSGGIEQDADIVLFLYRGYIYQMRAEDNKIDKLKKEGKIEEAQELHL
CCCCCCHHHHHHHHCCCCCCCCCEEEEEECCCEEEEECCCCHHHHHHHCCCHHHHHHHHE
KVNEERRIHKQNGSIEEAEIIVAKNRNGATGTVYTRFNAPFTRYEDMPIDSHLEENQETK
EECHHHHHHHCCCCCCEEEEEEEECCCCCCEEEEEEECCCCCCCCCCCCHHHHCCCCCCC
VDYDIVTT
CCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9252185 [H]