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The map label for this gene is priA [H]

Identifier: 108563430

GI number: 108563430

Start: 1059987

End: 1061846

Strand: Reverse

Name: priA [H]

Synonym: HPAG1_1005

Alternate gene names: 108563430

Gene position: 1061846-1059987 (Counterclockwise)

Preceding gene: 108563431

Following gene: 108563427

Centisome position: 66.52

GC content: 38.49

Gene sequence:

>1860_bases
ATGTTCTATTACTTGATCGCTCCTTTAAAAAATAAAACCCCCCCTTTAACTTACTTTTCTAAAGAACGACACCTTAAAGG
AGCGTTAGTCAATATCCCTTTAAGGAATAAAACGCTTTTGGGCGTCGTCCTTGAAGAAGTTTTAAAACCCTCTTTTGAAT
GCCTAGAATTGGAAAAAACCCCTTATTTTTTACTCCCCTTTCAAATAGAGCTCGCTATTTTTATCGCCCAATATTACTCG
GCTAATCTTTCTTCAGTCTTAAGCCTTTTTGCCCCTTTTAAAGAATGCGATTTAGTGGGGTTAGAAAAAATTGAGCCTAT
TCTTAATGCGTTAAGCCAAACGCAAACCAACGCTTTAAAAGAATTGCAAAAACATCCAGCAAGCTTGCTTTTTGGCGATA
CGGGTAGCGGGAAAACCGAAATTTATATGCATGCAATCGCTCAAACTTTGGAGCAAAAAAAAAGCGCTTTGTTGTTAGTG
CCAGAAATCGCCCTCACCCCTCAAATGCAGCAACGCCTTAAAAGGGTCTTTAAAGAAAATTTAGGCTTGTGGCATAGCAA
ACTCTCTCAAAATCAAAAAAAACAATTTTTAGAAAAGCTTTATTCGCAAGAAATCAAATTAGTGGTAGGCACACGAAGCG
CGTTGTTTTTACCCCTTAAGGAGTTGGGTTTAATCGTTGTAGATGAAGAGCATGACTTTTCTTATAAGTCCCATCAAAGC
CCTATGTATAACGCTAGGGATTTATGCTTGTATTTATCCCATAAATTCCCTATTCAAGTGGTTTTAGGCTCTGCTACGCC
AAGTTTGAATAGTTACAAACGCTTCAAAGATAAGGCTTTAGTGCGCTTAAAGGGGCGCTATACGCCTACGCAAAAAAACA
TTATTTTTGAAAAAACCGAGCGTTTTATCACGCCCAAACTCCTAGAAGCGCTGCAACAAGTCCTAGACAAAAACGAGCAA
GCCATTATTTTTGTGCCTACAAGGGCTAATTTCAAAACCTTGCTGTGTCAAAATTGCTATAAAAGCGTTCAATGCCCTTT
TTGCAGCGTGAATATGAGTTTGCATTTAAAAACCAACAAACTCATGTGCCATTATTGCCATTTTTCAAGCCCTATCCCTA
AAATTTGCAACGAGTGTCAAAGCGAAGTTTTAGTGGGTAAAAGGATAGGCACCATGCAAGTGTTGAACGAGTTGGAAGGC
CTTTTGAAAGGCGCTAAAATAGCGATTTTAGATAAAGATCACACCAGCACGCCAAAAAAACTCCACAATATTTTAAACGA
TTTCAACGCTCAAAAAACGAATATCTTAATCGGCACTCAAATGATAAGCAAAGGGCATGATTACGCTAAAGTGAGTTTAG
CGGTTGTTTTAGGCATAGACAATATCATTAAATCTAATAGTTACAGGGCTTTAGAAGAAGGCGTGTCGTTACTTTATCAA
ATCGCTGGGAGGAGCGCTAGGCAAATTTCTGGCCAAGTGTTCATTCAAAGCACCGAAACCGATCTATTAAAAAATTTCTT
AGAAGATTATGAAGATTTTTTACAATACGAATTGCAAGAAAGGTGCGAACTCTACCCGCCTTTTTCAAGGCTGTGTTTGT
TAGAGTTTAAGCATAAAAACGAAGAAAAAGCCCAACAATTGAGCCTAAAAGCCTCCCAAACCCTTTCTTTGTGTTTAGAA
AAGGGCGTAACGCTCTCTAATTTCAAAGCGCCCATTGAAAAAATCGCCTCTTCTTACCGCTACCTTATTTTGTTGCGTTC
CAAAAACCCTTTAAGCCTAATCAAAAGCGTGCATGCATTTTTAAAAACCGCCCCTAATATCCCTTGCAGCGTGAACATGG
ACCCTATAGATATTTTTTAA

Upstream 100 bases:

>100_bases
AATTAAACGCCTTAGAGCTTTTGAAGGATTATTTGCATGAAAGTTGTCAAAACGAGTATTTGCATAATGAACTCCAAAAG
CTTTTAGAAAAAATCTCATC

Downstream 100 bases:

>100_bases
AAACTTAAGTTTTCTATATTATTTCAAAAAACTCATGTTTTTCTAGCGACTAACAGCGTGAAATTCACCCATTTAAACAC
CGCTTCCACATGCTTAAACC

Product: primosome assembly protein PriA

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 619; Mature: 619

Protein sequence:

>619_residues
MFYYLIAPLKNKTPPLTYFSKERHLKGALVNIPLRNKTLLGVVLEEVLKPSFECLELEKTPYFLLPFQIELAIFIAQYYS
ANLSSVLSLFAPFKECDLVGLEKIEPILNALSQTQTNALKELQKHPASLLFGDTGSGKTEIYMHAIAQTLEQKKSALLLV
PEIALTPQMQQRLKRVFKENLGLWHSKLSQNQKKQFLEKLYSQEIKLVVGTRSALFLPLKELGLIVVDEEHDFSYKSHQS
PMYNARDLCLYLSHKFPIQVVLGSATPSLNSYKRFKDKALVRLKGRYTPTQKNIIFEKTERFITPKLLEALQQVLDKNEQ
AIIFVPTRANFKTLLCQNCYKSVQCPFCSVNMSLHLKTNKLMCHYCHFSSPIPKICNECQSEVLVGKRIGTMQVLNELEG
LLKGAKIAILDKDHTSTPKKLHNILNDFNAQKTNILIGTQMISKGHDYAKVSLAVVLGIDNIIKSNSYRALEEGVSLLYQ
IAGRSARQISGQVFIQSTETDLLKNFLEDYEDFLQYELQERCELYPPFSRLCLLEFKHKNEEKAQQLSLKASQTLSLCLE
KGVTLSNFKAPIEKIASSYRYLILLRSKNPLSLIKSVHAFLKTAPNIPCSVNMDPIDIF

Sequences:

>Translated_619_residues
MFYYLIAPLKNKTPPLTYFSKERHLKGALVNIPLRNKTLLGVVLEEVLKPSFECLELEKTPYFLLPFQIELAIFIAQYYS
ANLSSVLSLFAPFKECDLVGLEKIEPILNALSQTQTNALKELQKHPASLLFGDTGSGKTEIYMHAIAQTLEQKKSALLLV
PEIALTPQMQQRLKRVFKENLGLWHSKLSQNQKKQFLEKLYSQEIKLVVGTRSALFLPLKELGLIVVDEEHDFSYKSHQS
PMYNARDLCLYLSHKFPIQVVLGSATPSLNSYKRFKDKALVRLKGRYTPTQKNIIFEKTERFITPKLLEALQQVLDKNEQ
AIIFVPTRANFKTLLCQNCYKSVQCPFCSVNMSLHLKTNKLMCHYCHFSSPIPKICNECQSEVLVGKRIGTMQVLNELEG
LLKGAKIAILDKDHTSTPKKLHNILNDFNAQKTNILIGTQMISKGHDYAKVSLAVVLGIDNIIKSNSYRALEEGVSLLYQ
IAGRSARQISGQVFIQSTETDLLKNFLEDYEDFLQYELQERCELYPPFSRLCLLEFKHKNEEKAQQLSLKASQTLSLCLE
KGVTLSNFKAPIEKIASSYRYLILLRSKNPLSLIKSVHAFLKTAPNIPCSVNMDPIDIF
>Mature_619_residues
MFYYLIAPLKNKTPPLTYFSKERHLKGALVNIPLRNKTLLGVVLEEVLKPSFECLELEKTPYFLLPFQIELAIFIAQYYS
ANLSSVLSLFAPFKECDLVGLEKIEPILNALSQTQTNALKELQKHPASLLFGDTGSGKTEIYMHAIAQTLEQKKSALLLV
PEIALTPQMQQRLKRVFKENLGLWHSKLSQNQKKQFLEKLYSQEIKLVVGTRSALFLPLKELGLIVVDEEHDFSYKSHQS
PMYNARDLCLYLSHKFPIQVVLGSATPSLNSYKRFKDKALVRLKGRYTPTQKNIIFEKTERFITPKLLEALQQVLDKNEQ
AIIFVPTRANFKTLLCQNCYKSVQCPFCSVNMSLHLKTNKLMCHYCHFSSPIPKICNECQSEVLVGKRIGTMQVLNELEG
LLKGAKIAILDKDHTSTPKKLHNILNDFNAQKTNILIGTQMISKGHDYAKVSLAVVLGIDNIIKSNSYRALEEGVSLLYQ
IAGRSARQISGQVFIQSTETDLLKNFLEDYEDFLQYELQERCELYPPFSRLCLLEFKHKNEEKAQQLSLKASQTLSLCLE
KGVTLSNFKAPIEKIASSYRYLILLRSKNPLSLIKSVHAFLKTAPNIPCSVNMDPIDIF

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=561, Percent_Identity=33.3333333333333, Blast_Score=280, Evalue=2e-76,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 70465; Mature: 70465

Theoretical pI: Translated: 9.32; Mature: 9.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFYYLIAPLKNKTPPLTYFSKERHLKGALVNIPLRNKTLLGVVLEEVLKPSFECLELEKT
CEEEEEECCCCCCCCCCEECCCCCCCCEEEECCCCCCHHHHHHHHHHHCCCCEEEEECCC
PYFLLPFQIELAIFIAQYYSANLSSVLSLFAPFKECDLVGLEKIEPILNALSQTQTNALK
CEEEEEHHHHHHHHHHHHHCCCHHHHHHHHCCHHHCCCCCHHHHHHHHHHHHHHHHHHHH
ELQKHPASLLFGDTGSGKTEIYMHAIAQTLEQKKSALLLVPEIALTPQMQQRLKRVFKEN
HHHHCCCCEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEECCHHCCHHHHHHHHHHHHHH
LGLWHSKLSQNQKKQFLEKLYSQEIKLVVGTRSALFLPLKELGLIVVDEEHDFSYKSHQS
HCHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCEEEEHHHCCEEEEECCCCCCCCCCCC
PMYNARDLCLYLSHKFPIQVVLGSATPSLNSYKRFKDKALVRLKGRYTPTQKNIIFEKTE
CCCCHHHEEEEECCCCCEEEEEECCCCCHHHHHHHHHHHHEEEECCCCCCCCCEEHHHHH
RFITPKLLEALQQVLDKNEQAIIFVPTRANFKTLLCQNCYKSVQCPFCSVNMSLHLKTNK
HHCCHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCEEEECEEEEEECCC
LMCHYCHFSSPIPKICNECQSEVLVGKRIGTMQVLNELEGLLKGAKIAILDKDHTSTPKK
EEEEEECCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCHHH
LHNILNDFNAQKTNILIGTQMISKGHDYAKVSLAVVLGIDNIIKSNSYRALEEGVSLLYQ
HHHHHHHCCCCCCEEEEEHHHHHCCCCHHHEEEEEEECHHHHHHCCCHHHHHHHHHHHHH
IAGRSARQISGQVFIQSTETDLLKNFLEDYEDFLQYELQERCELYPPFSRLCLLEFKHKN
HHCCHHHCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCC
EEKAQQLSLKASQTLSLCLEKGVTLSNFKAPIEKIASSYRYLILLRSKNPLSLIKSVHAF
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHH
LKTAPNIPCSVNMDPIDIF
HHCCCCCCEECCCCCCCCC
>Mature Secondary Structure
MFYYLIAPLKNKTPPLTYFSKERHLKGALVNIPLRNKTLLGVVLEEVLKPSFECLELEKT
CEEEEEECCCCCCCCCCEECCCCCCCCEEEECCCCCCHHHHHHHHHHHCCCCEEEEECCC
PYFLLPFQIELAIFIAQYYSANLSSVLSLFAPFKECDLVGLEKIEPILNALSQTQTNALK
CEEEEEHHHHHHHHHHHHHCCCHHHHHHHHCCHHHCCCCCHHHHHHHHHHHHHHHHHHHH
ELQKHPASLLFGDTGSGKTEIYMHAIAQTLEQKKSALLLVPEIALTPQMQQRLKRVFKEN
HHHHCCCCEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEECCHHCCHHHHHHHHHHHHHH
LGLWHSKLSQNQKKQFLEKLYSQEIKLVVGTRSALFLPLKELGLIVVDEEHDFSYKSHQS
HCHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCEEEEHHHCCEEEEECCCCCCCCCCCC
PMYNARDLCLYLSHKFPIQVVLGSATPSLNSYKRFKDKALVRLKGRYTPTQKNIIFEKTE
CCCCHHHEEEEECCCCCEEEEEECCCCCHHHHHHHHHHHHEEEECCCCCCCCCEEHHHHH
RFITPKLLEALQQVLDKNEQAIIFVPTRANFKTLLCQNCYKSVQCPFCSVNMSLHLKTNK
HHCCHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCEEEECEEEEEECCC
LMCHYCHFSSPIPKICNECQSEVLVGKRIGTMQVLNELEGLLKGAKIAILDKDHTSTPKK
EEEEEECCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCHHH
LHNILNDFNAQKTNILIGTQMISKGHDYAKVSLAVVLGIDNIIKSNSYRALEEGVSLLYQ
HHHHHHHCCCCCCEEEEEHHHHHCCCCHHHEEEEEEECHHHHHHCCCHHHHHHHHHHHHH
IAGRSARQISGQVFIQSTETDLLKNFLEDYEDFLQYELQERCELYPPFSRLCLLEFKHKN
HHCCHHHCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCC
EEKAQQLSLKASQTLSLCLEKGVTLSNFKAPIEKIASSYRYLILLRSKNPLSLIKSVHAF
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHH
LKTAPNIPCSVNMDPIDIF
HHCCCCCCEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]