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The map label for this gene is dadA [C]

Identifier: 108563351

GI number: 108563351

Start: 969884

End: 971116

Strand: Reverse

Name: dadA [C]

Synonym: HPAG1_0926

Alternate gene names: 108563351

Gene position: 971116-969884 (Counterclockwise)

Preceding gene: 108563352

Following gene: 108563350

Centisome position: 60.83

GC content: 40.47

Gene sequence:

>1233_bases
ATGAAAAAAGAAGTCGTGGTCATAGGCGGTGGGATCGTGGGGCTTTCTTGCGCGTATTCTATGCATAAATTAGGGCATAA
GGTTTGCGTGATTGAAAAAAGCGATGGCAAAAACGGCACTTCCTTTGGGAATGCTGGGCTTATTTCTGCGTTTAAAAAAG
CCCCACTCTCATGTCCTGGTGTGGTGTTAGACACCCTAAAACTCATGCTCAAAAACCAAGCCCCTTTAAAATTCCATTTT
GGGCTTAATTTAAAGCTCTACCAATGGATTTTAAAGTTTATGAAAAGCGCGAACGCCAAATCCACGCACCGCACCATGGC
GTTGTTTGAACGCTACGGGTGGCTGAGTATTGATATTTATCATCAAATGCTAAAAGACGGCATGGACTTTTGGTATAAAG
AAGATGGGCTTTTAATGATCTACACTTTAGAAGAAAGTTTTGAAAAAAAGCTTAAAACTTGCGATGACAGCGGCGCTTAT
AAAATCCTTAGCGCAAAAGAAACCAAAGAATACATGCCTATTGTTAATGACAATATCTGCGGGAGCGTGCTTTTGACCGA
AAACGCGCATGTGGATCCGGGCGAAGTGATGCGCTCTTTGCAAGAATATTTACAAAACGCGGGCGTGGAGTTTCTTTATA
ATGAAGAAGTGATTGATTTTGAGTTTAAAAATAACCTCATTGATGGCGTTATCACGCATAAGGAAAAAATCCAAGCAGAA
ACAATCATTCTAGCCACCGGGGCTAACCCCACCCTCATTAAAAAAACCAAAAACGATTTTTTAATGATGGGGGCTAAAGG
CTATAGTATCACCTTTAAAATGCCTGAAGAATTAAAACCCAAAACCTCTTCTTTATTTGCGGATATTTTCATGGCAATGA
CCCCACGAAGAGACACCGTTAGGATCACTTCTAAATTAGAATTAAACACCAACAACGCCCTCATTGATAAAGAGCAAATC
GCTAACATGAAAAAGAATTTAGCCGCTTTCACGCAGCCTTTTGAAATGAAAGACGCCATAGAATGGTGCGGTTTCAGACC
TTTAACCCCTAATGATATTCCTTATTTGGGCTATGACAAACGCTATAAAAACTTAATCCATGCGACAGGATTGGGGTGGC
TTGGTATCACTTTTGGCCCGGCCATTGGTAAAATCATCGCCAATTTAAGCCAAGACGGAGCGAATGAAAAAAATGCCGAT
ATTATGCTTTTTTCTGCATTTTTTAGGGATTAA

Upstream 100 bases:

>100_bases
TCCGGCTAGAGCGACCTTTCAAGTGGCTAAACTGCCTAAAGACGCTTTAGTAGAAATTGAAGCGATAGCCATTAGGTAAT
TTATTAAAGGGGCTATTAGC

Downstream 100 bases:

>100_bases
GGAATTTCTTTTTTAAACCCTAGTTTGTTAAGGAGCTTTTATGGAAACGATTGATTCGGTGGTGCGTTTATTATCCAATT
TTGTGTGGGGGATTCCCATG

Product: D-amino acid dehydrogenase

Products: 2-oxo acid; NH3; reduced acceptor

Alternate protein names: NA

Number of amino acids: Translated: 410; Mature: 410

Protein sequence:

>410_residues
MKKEVVVIGGGIVGLSCAYSMHKLGHKVCVIEKSDGKNGTSFGNAGLISAFKKAPLSCPGVVLDTLKLMLKNQAPLKFHF
GLNLKLYQWILKFMKSANAKSTHRTMALFERYGWLSIDIYHQMLKDGMDFWYKEDGLLMIYTLEESFEKKLKTCDDSGAY
KILSAKETKEYMPIVNDNICGSVLLTENAHVDPGEVMRSLQEYLQNAGVEFLYNEEVIDFEFKNNLIDGVITHKEKIQAE
TIILATGANPTLIKKTKNDFLMMGAKGYSITFKMPEELKPKTSSLFADIFMAMTPRRDTVRITSKLELNTNNALIDKEQI
ANMKKNLAAFTQPFEMKDAIEWCGFRPLTPNDIPYLGYDKRYKNLIHATGLGWLGITFGPAIGKIIANLSQDGANEKNAD
IMLFSAFFRD

Sequences:

>Translated_410_residues
MKKEVVVIGGGIVGLSCAYSMHKLGHKVCVIEKSDGKNGTSFGNAGLISAFKKAPLSCPGVVLDTLKLMLKNQAPLKFHF
GLNLKLYQWILKFMKSANAKSTHRTMALFERYGWLSIDIYHQMLKDGMDFWYKEDGLLMIYTLEESFEKKLKTCDDSGAY
KILSAKETKEYMPIVNDNICGSVLLTENAHVDPGEVMRSLQEYLQNAGVEFLYNEEVIDFEFKNNLIDGVITHKEKIQAE
TIILATGANPTLIKKTKNDFLMMGAKGYSITFKMPEELKPKTSSLFADIFMAMTPRRDTVRITSKLELNTNNALIDKEQI
ANMKKNLAAFTQPFEMKDAIEWCGFRPLTPNDIPYLGYDKRYKNLIHATGLGWLGITFGPAIGKIIANLSQDGANEKNAD
IMLFSAFFRD
>Mature_410_residues
MKKEVVVIGGGIVGLSCAYSMHKLGHKVCVIEKSDGKNGTSFGNAGLISAFKKAPLSCPGVVLDTLKLMLKNQAPLKFHF
GLNLKLYQWILKFMKSANAKSTHRTMALFERYGWLSIDIYHQMLKDGMDFWYKEDGLLMIYTLEESFEKKLKTCDDSGAY
KILSAKETKEYMPIVNDNICGSVLLTENAHVDPGEVMRSLQEYLQNAGVEFLYNEEVIDFEFKNNLIDGVITHKEKIQAE
TIILATGANPTLIKKTKNDFLMMGAKGYSITFKMPEELKPKTSSLFADIFMAMTPRRDTVRITSKLELNTNNALIDKEQI
ANMKKNLAAFTQPFEMKDAIEWCGFRPLTPNDIPYLGYDKRYKNLIHATGLGWLGITFGPAIGKIIANLSQDGANEKNAD
IMLFSAFFRD

Specific function: Oxidative Deamination Of D-Amino Acids. [C]

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Inner Membrane-Bound [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DadA oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI1787438, Length=400, Percent_Identity=26.25, Blast_Score=136, Evalue=2e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: 1.4.99.1

Molecular weight: Translated: 46149; Mature: 46149

Theoretical pI: Translated: 8.52; Mature: 8.52

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKEVVVIGGGIVGLSCAYSMHKLGHKVCVIEKSDGKNGTSFGNAGLISAFKKAPLSCPG
CCCEEEEEECCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCHHHHHHHHCCCCCCH
VVLDTLKLMLKNQAPLKFHFGLNLKLYQWILKFMKSANAKSTHRTMALFERYGWLSIDIY
HHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCEEHHHH
HQMLKDGMDFWYKEDGLLMIYTLEESFEKKLKTCDDSGAYKILSAKETKEYMPIVNDNIC
HHHHHCCHHHEECCCCEEEEEECCHHHHHHHHCCCCCCCEEEEECCCHHHCCCCCCCCCC
GSVLLTENAHVDPGEVMRSLQEYLQNAGVEFLYNEEVIDFEFKNNLIDGVITHKEKIQAE
CEEEEECCCCCCHHHHHHHHHHHHHHCCEEEEECCCEEEEEHHCCCHHHHHHHHHHCCEE
TIILATGANPTLIKKTKNDFLMMGAKGYSITFKMPEELKPKTSSLFADIFMAMTPRRDTV
EEEEEECCCCEEEEECCCCEEEEECCCEEEEEECCHHCCCHHHHHHHHHHHHCCCCCCEE
RITSKLELNTNNALIDKEQIANMKKNLAAFTQPFEMKDAIEWCGFRPLTPNDIPYLGYDK
EEEEEEEECCCCCEECHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCCCCCCCH
RYKNLIHATGLGWLGITFGPAIGKIIANLSQDGANEKNADIMLFSAFFRD
HHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCC
>Mature Secondary Structure
MKKEVVVIGGGIVGLSCAYSMHKLGHKVCVIEKSDGKNGTSFGNAGLISAFKKAPLSCPG
CCCEEEEEECCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCHHHHHHHHCCCCCCH
VVLDTLKLMLKNQAPLKFHFGLNLKLYQWILKFMKSANAKSTHRTMALFERYGWLSIDIY
HHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCEEHHHH
HQMLKDGMDFWYKEDGLLMIYTLEESFEKKLKTCDDSGAYKILSAKETKEYMPIVNDNIC
HHHHHCCHHHEECCCCEEEEEECCHHHHHHHHCCCCCCCEEEEECCCHHHCCCCCCCCCC
GSVLLTENAHVDPGEVMRSLQEYLQNAGVEFLYNEEVIDFEFKNNLIDGVITHKEKIQAE
CEEEEECCCCCCHHHHHHHHHHHHHHCCEEEEECCCEEEEEHHCCCHHHHHHHHHHCCEE
TIILATGANPTLIKKTKNDFLMMGAKGYSITFKMPEELKPKTSSLFADIFMAMTPRRDTV
EEEEEECCCCEEEEECCCCEEEEECCCEEEEEECCHHCCCHHHHHHHHHHHHCCCCCCEE
RITSKLELNTNNALIDKEQIANMKKNLAAFTQPFEMKDAIEWCGFRPLTPNDIPYLGYDK
EEEEEEEECCCCCEECHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCCCCCCCH
RYKNLIHATGLGWLGITFGPAIGKIIANLSQDGANEKNADIMLFSAFFRD
HHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Iron [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 40 {D-proline}} 0.13 {K3Fe(CN)6}} 0.63 {dichloroindophenol}} 0.01 {dichloroindophenol}} 11 {D-methionine}} 6 {D-phenylalanine}} 12 {D-alpha-aminobutyrate}} 0.15 {cytochrome} 19 {D-serine}} 29 {D-alanine}} 6.4 {D-alanine}} 5.3 {D

Substrates: D-amino acid; H2O; acceptor

Specific reaction: a D-amino acid + H2O + acceptor = a 2-oxo acid + NH3 + reduced acceptor

General reaction: Oxidative deamination; Redox reaction [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]