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The map label for this gene is greA [H]

Identifier: 108563274

GI number: 108563274

Start: 883271

End: 883765

Strand: Reverse

Name: greA [H]

Synonym: HPAG1_0849

Alternate gene names: 108563274

Gene position: 883765-883271 (Counterclockwise)

Preceding gene: 108563275

Following gene: 108563273

Centisome position: 55.36

GC content: 38.79

Gene sequence:

>495_bases
ATGAATAAAGAACCTATGAGTATGCATGGATACAATAAGATTTGCGCGGAATTGAAGCAATTAAAAGAGGTGGAACGGCC
TAATATTGTGAAAGAAATTGATATTGCTAGAGGGCATGGGGACTTGAAAGAAAACGCTGAATACCATGCCGCTAAAGAAA
AACAACGCTTCATTGAAGCGAGAATCGTGGATTTAAGCGAAATTGTCGCTAACGCTCAAGTGATTGATCCGAGCGTTTTA
GCTCATAATAAAGTGAGTTTTGGCAGCACGATTAAAATCCTTAATTTAGACAACGATAAAGAGTTTTCTTATACGATAGT
AGGGAGCGTGGAGAGCGATCCGGCTAAAGGGTTGATCTCTTTTGGTTCGCCGATCGCTAAGAGCTTGATAGGTAAGAGTA
AGGGCGATGCGGTGAGCATTCAATTGCCTAATGGCGAAAGCGATTTTGAAATTTTAGACATTTATTATAAAGAGATTTGT
TTTGATGAAAATTAA

Upstream 100 bases:

>100_bases
GCCAGTGGGAGCGCGAGAAAAATCGCTAATGAAATGGCTTTTTTGCTGAATTTAACTTAATTTTTACTATAATCTCTACT
GAATAAGCTTAGGAGATTAA

Downstream 100 bases:

>100_bases
AATCCAAAAAATCCACCCAAACGCCCTTATCCCTAAATACCAAACCGAGGGTTCTTCAGGTTTTGATTTGCACGCTGTAG
AAGAAGTAACGATCAAACCT

Product: transcription elongation factor GreA

Products: NA

Alternate protein names: Transcript cleavage factor greA [H]

Number of amino acids: Translated: 164; Mature: 164

Protein sequence:

>164_residues
MNKEPMSMHGYNKICAELKQLKEVERPNIVKEIDIARGHGDLKENAEYHAAKEKQRFIEARIVDLSEIVANAQVIDPSVL
AHNKVSFGSTIKILNLDNDKEFSYTIVGSVESDPAKGLISFGSPIAKSLIGKSKGDAVSIQLPNGESDFEILDIYYKEIC
FDEN

Sequences:

>Translated_164_residues
MNKEPMSMHGYNKICAELKQLKEVERPNIVKEIDIARGHGDLKENAEYHAAKEKQRFIEARIVDLSEIVANAQVIDPSVL
AHNKVSFGSTIKILNLDNDKEFSYTIVGSVESDPAKGLISFGSPIAKSLIGKSKGDAVSIQLPNGESDFEILDIYYKEIC
FDEN
>Mature_164_residues
MNKEPMSMHGYNKICAELKQLKEVERPNIVKEIDIARGHGDLKENAEYHAAKEKQRFIEARIVDLSEIVANAQVIDPSVL
AHNKVSFGSTIKILNLDNDKEFSYTIVGSVESDPAKGLISFGSPIAKSLIGKSKGDAVSIQLPNGESDFEILDIYYKEIC
FDEN

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family [H]

Homologues:

Organism=Escherichia coli, GI87082225, Length=157, Percent_Identity=50.3184713375796, Blast_Score=153, Evalue=5e-39,
Organism=Escherichia coli, GI87082261, Length=155, Percent_Identity=34.1935483870968, Blast_Score=86, Evalue=1e-18,

Paralogues:

None

Copy number: 259 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018151
- InterPro:   IPR006359
- InterPro:   IPR001437
- InterPro:   IPR022691 [H]

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N [H]

EC number: NA

Molecular weight: Translated: 18227; Mature: 18227

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: PS00829 GREAB_1 ; PS00830 GREAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKEPMSMHGYNKICAELKQLKEVERPNIVKEIDIARGHGDLKENAEYHAAKEKQRFIEA
CCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCHHCCHHHHHHHHHHHHHH
RIVDLSEIVANAQVIDPSVLAHNKVSFGSTIKILNLDNDKEFSYTIVGSVESDPAKGLIS
HHHHHHHHHHCCCCCCCHHHHCCCCCCCCEEEEEECCCCCCEEEEEEECCCCCCCCHHHH
FGSPIAKSLIGKSKGDAVSIQLPNGESDFEILDIYYKEICFDEN
CCCHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNKEPMSMHGYNKICAELKQLKEVERPNIVKEIDIARGHGDLKENAEYHAAKEKQRFIEA
CCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCHHCCHHHHHHHHHHHHHH
RIVDLSEIVANAQVIDPSVLAHNKVSFGSTIKILNLDNDKEFSYTIVGSVESDPAKGLIS
HHHHHHHHHHCCCCCCCHHHHCCCCCCCCEEEEEECCCCCCEEEEEEECCCCCCCCHHHH
FGSPIAKSLIGKSKGDAVSIQLPNGESDFEILDIYYKEICFDEN
CCCHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA