Click here to switch to the map view.

The map label for this gene is tilS [H]

Identifier: 108563137

GI number: 108563137

Start: 750435

End: 751451

Strand: Reverse

Name: tilS [H]

Synonym: HPAG1_0712

Alternate gene names: 108563137

Gene position: 751451-750435 (Counterclockwise)

Preceding gene: 108563138

Following gene: 108563136

Centisome position: 47.07

GC content: 37.66

Gene sequence:

>1017_bases
TTGACAGCGCGAGATTTTAAAACCTATTTAGAGCCTTTAAGAGAGGGGAAAAATTTATTGGGTTTTTCGGGTGGGTTGGA
TTCCACTTGCTTGTTCCATCTTTTAGTTGGAAAAAACATCGCTTTTGACATCGCTTTAGTGGATTATAACACGCAAAAAC
AACGCCTTGAAATCATCCAACACGCCCAAAAACTCGCCCAAGCGCACCATAAAAAATGCTATATCCATTACGCCCCAAAG
ATTGAGCGCAATTTTGAAATGCAAGCGAGGAAGATCCGTTATGATTTTTTTGAAACCCTAATGAGAGAGTATTCTTACAA
GCATTTGATTTTAGCGCACCACTTGAATGACAGGCTGGAATGGTTTTTGATGCAATTAAGCAAAGGAGCCGGGCTAAACA
CGCTTTTAAGCTTTCAAGCTTATGAAAAAAGAGGGTTTTATGCGATCGTTCGCCCCTTGCTCTACACCCCTAAAGACACC
CTTAAAACGCTCGCTAAAGATCAAAAATTTTTTGAAGATGATTCTAATTCTTCTTTAAAATTCAAACGCAATTTTTTCAG
GAAACATTACGCTAACGCCTTGATGCAAGATTATTCTAAGGGCATTATCCAAAGTTTTAAATTTTTGGATCAAGAAAAAG
AGCGGCTTTATCCTTTAACGCCCGTTTCACAAATGCATGGGATCACTTTTTTTAAGCATTCGCAAAACGCGCTGTTTATG
GTGGATAAAATCTTAAAGCAAAAGGGGTATGTGTTGAGCTTTTCTCAAAAAGAAGAAATCAAGCGCCATTTTTTTAGCCT
AGAAATCGCTCAAAAATTCATCATTGAAAGCAATAAAGAGCATGTTTTTATCGCTCTTAAACCCCCAAAAACTTTAAGCA
TGCCAAAGGATTTTAAAGAAAAAGCCAGGAGGTTGGATATCCCTAAACGCTTACGGCCTGTTTTATACGCAGAGTTTTTA
AAACAACCAACGCATGATTTTTTAACCCGTTTCAAACAGAGTTTAACGGATCTATAA

Upstream 100 bases:

>100_bases
AAATTTTAGGCATAGAAGGCGCAAGCTTACTCAAAGGCATTTTAACCCACAAGGAAACTTCATAAAAGCACCATCAATTC
AGCATGCGTTTAACACCGAT

Downstream 100 bases:

>100_bases
AAGAGCTTCATTTCAATCAAGCGATAAGTTTTTAAAGCGCTTTTTAACCTTGATTGTGTCAAAATACAGCAAAAAACTTT
GATATGAGAGCGTATGGACT

Product: hypothetical protein

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 338; Mature: 337

Protein sequence:

>338_residues
MTARDFKTYLEPLREGKNLLGFSGGLDSTCLFHLLVGKNIAFDIALVDYNTQKQRLEIIQHAQKLAQAHHKKCYIHYAPK
IERNFEMQARKIRYDFFETLMREYSYKHLILAHHLNDRLEWFLMQLSKGAGLNTLLSFQAYEKRGFYAIVRPLLYTPKDT
LKTLAKDQKFFEDDSNSSLKFKRNFFRKHYANALMQDYSKGIIQSFKFLDQEKERLYPLTPVSQMHGITFFKHSQNALFM
VDKILKQKGYVLSFSQKEEIKRHFFSLEIAQKFIIESNKEHVFIALKPPKTLSMPKDFKEKARRLDIPKRLRPVLYAEFL
KQPTHDFLTRFKQSLTDL

Sequences:

>Translated_338_residues
MTARDFKTYLEPLREGKNLLGFSGGLDSTCLFHLLVGKNIAFDIALVDYNTQKQRLEIIQHAQKLAQAHHKKCYIHYAPK
IERNFEMQARKIRYDFFETLMREYSYKHLILAHHLNDRLEWFLMQLSKGAGLNTLLSFQAYEKRGFYAIVRPLLYTPKDT
LKTLAKDQKFFEDDSNSSLKFKRNFFRKHYANALMQDYSKGIIQSFKFLDQEKERLYPLTPVSQMHGITFFKHSQNALFM
VDKILKQKGYVLSFSQKEEIKRHFFSLEIAQKFIIESNKEHVFIALKPPKTLSMPKDFKEKARRLDIPKRLRPVLYAEFL
KQPTHDFLTRFKQSLTDL
>Mature_337_residues
TARDFKTYLEPLREGKNLLGFSGGLDSTCLFHLLVGKNIAFDIALVDYNTQKQRLEIIQHAQKLAQAHHKKCYIHYAPKI
ERNFEMQARKIRYDFFETLMREYSYKHLILAHHLNDRLEWFLMQLSKGAGLNTLLSFQAYEKRGFYAIVRPLLYTPKDTL
KTLAKDQKFFEDDSNSSLKFKRNFFRKHYANALMQDYSKGIIQSFKFLDQEKERLYPLTPVSQMHGITFFKHSQNALFMV
DKILKQKGYVLSFSQKEEIKRHFFSLEIAQKFIIESNKEHVFIALKPPKTLSMPKDFKEKARRLDIPKRLRPVLYAEFLK
QPTHDFLTRFKQSLTDL

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=190, Percent_Identity=28.9473684210526, Blast_Score=75, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012795
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3 [H]

EC number: NA

Molecular weight: Translated: 40141; Mature: 40010

Theoretical pI: Translated: 10.28; Mature: 10.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTARDFKTYLEPLREGKNLLGFSGGLDSTCLFHLLVGKNIAFDIALVDYNTQKQRLEIIQ
CCCHHHHHHHHHHHCCCCEECCCCCCCHHHHHHHHHCCCCEEEEEEEECCCHHHHHHHHH
HAQKLAQAHHKKCYIHYAPKIERNFEMQARKIRYDFFETLMREYSYKHLILAHHLNDRLE
HHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
WFLMQLSKGAGLNTLLSFQAYEKRGFYAIVRPLLYTPKDTLKTLAKDQKFFEDDSNSSLK
HHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCHH
FKRNFFRKHYANALMQDYSKGIIQSFKFLDQEKERLYPLTPVSQMHGITFFKHSQNALFM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCEEEEECCCHHHH
VDKILKQKGYVLSFSQKEEIKRHFFSLEIAQKFIIESNKEHVFIALKPPKTLSMPKDFKE
HHHHHHHCCCEEECCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHH
KARRLDIPKRLRPVLYAEFLKQPTHDFLTRFKQSLTDL
HHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TARDFKTYLEPLREGKNLLGFSGGLDSTCLFHLLVGKNIAFDIALVDYNTQKQRLEIIQ
CCHHHHHHHHHHHCCCCEECCCCCCCHHHHHHHHHCCCCEEEEEEEECCCHHHHHHHHH
HAQKLAQAHHKKCYIHYAPKIERNFEMQARKIRYDFFETLMREYSYKHLILAHHLNDRLE
HHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
WFLMQLSKGAGLNTLLSFQAYEKRGFYAIVRPLLYTPKDTLKTLAKDQKFFEDDSNSSLK
HHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCHH
FKRNFFRKHYANALMQDYSKGIIQSFKFLDQEKERLYPLTPVSQMHGITFFKHSQNALFM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCEEEEECCCHHHH
VDKILKQKGYVLSFSQKEEIKRHFFSLEIAQKFIIESNKEHVFIALKPPKTLSMPKDFKE
HHHHHHHCCCEEECCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHH
KARRLDIPKRLRPVLYAEFLKQPTHDFLTRFKQSLTDL
HHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]