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The map label for this gene is ansA [H]

Identifier: 108563133

GI number: 108563133

Start: 745165

End: 746163

Strand: Direct

Name: ansA [H]

Synonym: HPAG1_0708

Alternate gene names: 108563133

Gene position: 745165-746163 (Clockwise)

Preceding gene: 108563131

Following gene: 108563146

Centisome position: 46.68

GC content: 45.85

Gene sequence:

>999_bases
ATGGCTCAAAATTTACCCACCATTGCTTTACTGGCGACAGGGGGGACGATTGCAGGGAGCGGCACGAGCGCGAGTTCGGG
TAGTTATAAGAGTGGTGAGTTGGGCATCAAAGAGCTTCTTAAAGCTATCCCTAGTCTTAATAGACTCGCTCGCATTCAAG
GGGAACAGATTTCTAACATCGGCTCACAAGACATGAATGAAGAAATATGGTTCAAACTTGCTAAACGCGCCCAAGAATTG
CTCAATGATAGCCGCATTCAAGGCGTTGTCATCACGCATGGCACGGACACTTTAGAAGAGAGCGCGTATTTTTTAAATTT
AGTTTTACGCTCCACAAAACCGGTGGTGTTAGTGGGAGCGATGCGTAACGCTTCTTCTTTGAGCGCGGATGGGGCTTTGA
ATTTGTATAACGCGCTGAGCGTAGCGCTCAATGAAAAAAGCGCGAATAAAGGCGTGTTGGTGGTGATGGACGATAATATT
TTTAGCGCTAGGGAAGTGGTTAAAACGCACACCACCCACACTTCCACCTTTAAAGCCTTAAATAGTGGCGCGATAGGGAG
TGTGTATTATGGCAAAACGCGCTATTACATGCAGCCTTTGAGAAAACACACCACAGAGAGCGAATTTTCTCTTTCACAAC
TCAAAACCCCCTTGCCTAAAGTGGATATTATTTACACGCATGCCGGCATGACCCCTGATCTATTCCAAGCGAGCCTAAAC
TCGCATGCAAAAGGCGTTGTGATAGCCGGGGTGGGTAATGGGAATGTGAGCACTGGGTTTTTAAAAGCGATGCAAGAAGC
GAGCCAAATGGGGGTGGTTATTGTTCGTTCTAGCAGGGTAGGCAGCGGTGGAGTTACTTCAGGCGAGATTGATGACAAGG
CTTATGGCTTCATCACAAGCGACAATTTAAACCCCCAAAAAGCCAGAGTGCTTTTACAACTCGCTTTAACTAAAACAAAT
GATAAGGCAAAAATCCAAGAAATGTTTGAAGAGTATTAA

Upstream 100 bases:

>100_bases
GTTTTACTATACTATAAAATCTTTTAATTTCAAAAGGTGGTCCATAATGAGAATGTTTTTGAAATTATTGATTCTTTTAT
TTTGTTTGAAGGGGCAAGTT

Downstream 100 bases:

>100_bases
ACGATTTTCTTAGATTACCCAATTATCAAAGATAATTGGGTGATTTGGTTTATTTTGTTTTTGACTTTATTTTCATCAAA
TATTGATAACTATCAAAATA

Product: L-asparaginase II

Products: NA

Alternate protein names: L-ASNase; L-asparagine amidohydrolase [H]

Number of amino acids: Translated: 332; Mature: 331

Protein sequence:

>332_residues
MAQNLPTIALLATGGTIAGSGTSASSGSYKSGELGIKELLKAIPSLNRLARIQGEQISNIGSQDMNEEIWFKLAKRAQEL
LNDSRIQGVVITHGTDTLEESAYFLNLVLRSTKPVVLVGAMRNASSLSADGALNLYNALSVALNEKSANKGVLVVMDDNI
FSAREVVKTHTTHTSTFKALNSGAIGSVYYGKTRYYMQPLRKHTTESEFSLSQLKTPLPKVDIIYTHAGMTPDLFQASLN
SHAKGVVIAGVGNGNVSTGFLKAMQEASQMGVVIVRSSRVGSGGVTSGEIDDKAYGFITSDNLNPQKARVLLQLALTKTN
DKAKIQEMFEEY

Sequences:

>Translated_332_residues
MAQNLPTIALLATGGTIAGSGTSASSGSYKSGELGIKELLKAIPSLNRLARIQGEQISNIGSQDMNEEIWFKLAKRAQEL
LNDSRIQGVVITHGTDTLEESAYFLNLVLRSTKPVVLVGAMRNASSLSADGALNLYNALSVALNEKSANKGVLVVMDDNI
FSAREVVKTHTTHTSTFKALNSGAIGSVYYGKTRYYMQPLRKHTTESEFSLSQLKTPLPKVDIIYTHAGMTPDLFQASLN
SHAKGVVIAGVGNGNVSTGFLKAMQEASQMGVVIVRSSRVGSGGVTSGEIDDKAYGFITSDNLNPQKARVLLQLALTKTN
DKAKIQEMFEEY
>Mature_331_residues
AQNLPTIALLATGGTIAGSGTSASSGSYKSGELGIKELLKAIPSLNRLARIQGEQISNIGSQDMNEEIWFKLAKRAQELL
NDSRIQGVVITHGTDTLEESAYFLNLVLRSTKPVVLVGAMRNASSLSADGALNLYNALSVALNEKSANKGVLVVMDDNIF
SAREVVKTHTTHTSTFKALNSGAIGSVYYGKTRYYMQPLRKHTTESEFSLSQLKTPLPKVDIIYTHAGMTPDLFQASLNS
HAKGVVIAGVGNGNVSTGFLKAMQEASQMGVVIVRSSRVGSGGVTSGEIDDKAYGFITSDNLNPQKARVLLQLALTKTND
KAKIQEMFEEY

Specific function: Unknown

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1789327, Length=329, Percent_Identity=49.2401215805471, Blast_Score=315, Evalue=3e-87,
Organism=Escherichia coli, GI1788065, Length=288, Percent_Identity=28.125, Blast_Score=86, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6323189, Length=331, Percent_Identity=38.0664652567976, Blast_Score=206, Evalue=3e-54,
Organism=Saccharomyces cerevisiae, GI6323187, Length=331, Percent_Identity=38.0664652567976, Blast_Score=206, Evalue=3e-54,
Organism=Saccharomyces cerevisiae, GI6323186, Length=331, Percent_Identity=38.0664652567976, Blast_Score=206, Evalue=3e-54,
Organism=Saccharomyces cerevisiae, GI6323184, Length=331, Percent_Identity=38.0664652567976, Blast_Score=206, Evalue=3e-54,
Organism=Saccharomyces cerevisiae, GI6320527, Length=332, Percent_Identity=33.1325301204819, Blast_Score=181, Evalue=1e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004550
- InterPro:   IPR006034
- InterPro:   IPR020827 [H]

Pfam domain/function: PF00710 Asparaginase [H]

EC number: =3.5.1.1 [H]

Molecular weight: Translated: 35622; Mature: 35491

Theoretical pI: Translated: 9.52; Mature: 9.52

Prosite motif: PS00144 ASN_GLN_ASE_1 ; PS00917 ASN_GLN_ASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQNLPTIALLATGGTIAGSGTSASSGSYKSGELGIKELLKAIPSLNRLARIQGEQISNI
CCCCCCEEEEEEECCEEECCCCCCCCCCCCCCCCCHHHHHHHCCCHHHHHHHCCHHHHCC
GSQDMNEEIWFKLAKRAQELLNDSRIQGVVITHGTDTLEESAYFLNLVLRSTKPVVLVGA
CCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCEEEEEE
MRNASSLSADGALNLYNALSVALNEKSANKGVLVVMDDNIFSAREVVKTHTTHTSTFKAL
CCCCCCCCCCCCHHHHHHHHHEECCCCCCCCEEEEECCCCHHHHHHHHHCCCCHHHHHHH
NSGAIGSVYYGKTRYYMQPLRKHTTESEFSLSQLKTPLPKVDIIYTHAGMTPDLFQASLN
CCCCCCEEEECCHHHHHHHHHHCCCCCCCCHHHHCCCCCCEEEEEEECCCCHHHHHHHHC
SHAKGVVIAGVGNGNVSTGFLKAMQEASQMGVVIVRSSRVGSGGVTSGEIDDKAYGFITS
CCCCEEEEEECCCCCCHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCEEEEEEC
DNLNPQKARVLLQLALTKTNDKAKIQEMFEEY
CCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCC
>Mature Secondary Structure 
AQNLPTIALLATGGTIAGSGTSASSGSYKSGELGIKELLKAIPSLNRLARIQGEQISNI
CCCCCEEEEEEECCEEECCCCCCCCCCCCCCCCCHHHHHHHCCCHHHHHHHCCHHHHCC
GSQDMNEEIWFKLAKRAQELLNDSRIQGVVITHGTDTLEESAYFLNLVLRSTKPVVLVGA
CCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCEEEEEE
MRNASSLSADGALNLYNALSVALNEKSANKGVLVVMDDNIFSAREVVKTHTTHTSTFKAL
CCCCCCCCCCCCHHHHHHHHHEECCCCCCCCEEEEECCCCHHHHHHHHHCCCCHHHHHHH
NSGAIGSVYYGKTRYYMQPLRKHTTESEFSLSQLKTPLPKVDIIYTHAGMTPDLFQASLN
CCCCCCEEEECCHHHHHHHHHHCCCCCCCCHHHHCCCCCCEEEEEEECCCCHHHHHHHHC
SHAKGVVIAGVGNGNVSTGFLKAMQEASQMGVVIVRSSRVGSGGVTSGEIDDKAYGFITS
CCCCEEEEEECCCCCCHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCEEEEEEC
DNLNPQKARVLLQLALTKTNDKAKIQEMFEEY
CCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]