Click here to switch to the map view.

The map label for this gene is cofH [H]

Identifier: 108563064

GI number: 108563064

Start: 664161

End: 665243

Strand: Direct

Name: cofH [H]

Synonym: HPAG1_0639

Alternate gene names: 108563064

Gene position: 664161-665243 (Clockwise)

Preceding gene: 108563060

Following gene: 108563065

Centisome position: 41.6

GC content: 37.77

Gene sequence:

>1083_bases
ATGGACTTTTTAGAAAAAGTATTAGACAATCAAGTTACTGAAAGTAAAGAATTAGTGAAGCTTTATGATTATGATTTATA
CACGCTAGGGGAAGCAGCGGATCACATGCGCCAGAACATGCACCAAAAAATCGTGTATTTTAATGTCAATAGGCATTTAA
ACCCTAGCAATATTTGCACAGACGCTTGCAAATTTTGTGCTTTTTCAGCCCACAGAAAAAACCCTAACCCTTATGAAATG
AGTTTAGAAGAAATTATAGAAAAGGTTAAAAACTCCTACAACAAGGGGATTAAAGAAGTCCATATCGTGAGCGCTCATAA
CCCTAATTACTCTTATGAATGGTATTTAAAGGTGTTTGAAACCATCAAGCAAGAAATGCCTAACTTGCATTTAAAGGCCA
TGACCGCTGCAGAAGTGCATTTTTTAAGTGTTAAATTCAACAAACCTTTTGAATTGGTGCTAGAAGACATGCTCAAAGCT
GGGGTGGATTCCATGCCTGGTGGGGGGGCGGAGATTTTTGATGAAGAAGTTAGGCGTAAAATCTGTAAGGGTAAGGTGGG
ATCTTCTCGGTGGTTAGAAATCCATGCTTATTGGCACAAATTAGGCAAAATGAGTAACGCTACCATGCTTTTTGGGCATA
TTGAAAATAAAATCCATCGCATCGATCACATGCTGAGAATCAAAAAAATCCAAAGCCCTAAAAATAAAGTGGAAAATAAA
GAAGGGGGTTTTAACGCTTTTATCCCCTTGTTGTATCAAAAAGAAAACAATTATTTGAAAGTGGAAAAATCCCCTAGCGC
GATAGAAATCTTAAAAACCATCGCCATATCTCGCATTCTTTTAAACAATATCCCCCACATTAAAGCTTATTGGGCGACTT
TGGGCTTGAATTTGGCTTTAGTGGCTCAAGAATTTGGCGCTAACGATTTAGACGGCACGATAGAAATAGAGAGCATTCAA
AGCGCGGCTGGCGCGAAGAGCCGGCATGGTTTAGAAAAAGAAGATTTGATATTTAAAATCAAGGACGCTGGTTTTGTTGC
GGTAGAAAGGGATAGTTTGTATAATTTTATACAGAAATTTTAA

Upstream 100 bases:

>100_bases
AGGGGGGATTAAAAGGGATATTTTTATATAATATATAAAAAGCATTCTTCAATCTAAAAATGCTACAATGTTTATCTTTA
AAATGAAAGGCGATTTAACC

Downstream 100 bases:

>100_bases
TAATTTTTAGCGTTTTTAAGAATGATTAGTTATAATAACGCTACTAACAACACTCAAGCTAAAATCTATTAAGGAAATCA
GTATTAAAAAATTAATTCTA

Product: hypothetical protein

Products: NA

Alternate protein names: 7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase subunit 2 [H]

Number of amino acids: Translated: 360; Mature: 360

Protein sequence:

>360_residues
MDFLEKVLDNQVTESKELVKLYDYDLYTLGEAADHMRQNMHQKIVYFNVNRHLNPSNICTDACKFCAFSAHRKNPNPYEM
SLEEIIEKVKNSYNKGIKEVHIVSAHNPNYSYEWYLKVFETIKQEMPNLHLKAMTAAEVHFLSVKFNKPFELVLEDMLKA
GVDSMPGGGAEIFDEEVRRKICKGKVGSSRWLEIHAYWHKLGKMSNATMLFGHIENKIHRIDHMLRIKKIQSPKNKVENK
EGGFNAFIPLLYQKENNYLKVEKSPSAIEILKTIAISRILLNNIPHIKAYWATLGLNLALVAQEFGANDLDGTIEIESIQ
SAAGAKSRHGLEKEDLIFKIKDAGFVAVERDSLYNFIQKF

Sequences:

>Translated_360_residues
MDFLEKVLDNQVTESKELVKLYDYDLYTLGEAADHMRQNMHQKIVYFNVNRHLNPSNICTDACKFCAFSAHRKNPNPYEM
SLEEIIEKVKNSYNKGIKEVHIVSAHNPNYSYEWYLKVFETIKQEMPNLHLKAMTAAEVHFLSVKFNKPFELVLEDMLKA
GVDSMPGGGAEIFDEEVRRKICKGKVGSSRWLEIHAYWHKLGKMSNATMLFGHIENKIHRIDHMLRIKKIQSPKNKVENK
EGGFNAFIPLLYQKENNYLKVEKSPSAIEILKTIAISRILLNNIPHIKAYWATLGLNLALVAQEFGANDLDGTIEIESIQ
SAAGAKSRHGLEKEDLIFKIKDAGFVAVERDSLYNFIQKF
>Mature_360_residues
MDFLEKVLDNQVTESKELVKLYDYDLYTLGEAADHMRQNMHQKIVYFNVNRHLNPSNICTDACKFCAFSAHRKNPNPYEM
SLEEIIEKVKNSYNKGIKEVHIVSAHNPNYSYEWYLKVFETIKQEMPNLHLKAMTAAEVHFLSVKFNKPFELVLEDMLKA
GVDSMPGGGAEIFDEEVRRKICKGKVGSSRWLEIHAYWHKLGKMSNATMLFGHIENKIHRIDHMLRIKKIQSPKNKVENK
EGGFNAFIPLLYQKENNYLKVEKSPSAIEILKTIAISRILLNNIPHIKAYWATLGLNLALVAQEFGANDLDGTIEIESIQ
SAAGAKSRHGLEKEDLIFKIKDAGFVAVERDSLYNFIQKF

Specific function: Catalyzes the radical-mediated transfer of the hydroxybenzyl group from 4-hydroxyphenylpyruvate (HPP) to 5-amino- 6-ribitylamino-2,4(1H,3H)-pyrimidinedione to form 7,8-didemethyl- 8-hydroxy-5-deazariboflavin (FO) [H]

COG id: COG1060

COG function: function code HR; Thiamine biosynthesis enzyme ThiH and related uncharacterized enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. CofH family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR019940
- InterPro:   IPR006638
- InterPro:   IPR020050
- InterPro:   IPR005244
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 41365; Mature: 41365

Theoretical pI: Translated: 8.30; Mature: 8.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFLEKVLDNQVTESKELVKLYDYDLYTLGEAADHMRQNMHQKIVYFNVNRHLNPSNICT
CCHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHEEEEEECCCCCCHHHHHH
DACKFCAFSAHRKNPNPYEMSLEEIIEKVKNSYNKGIKEVHIVSAHNPNYSYEWYLKVFE
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHH
TIKQEMPNLHLKAMTAAEVHFLSVKFNKPFELVLEDMLKAGVDSMPGGGAEIFDEEVRRK
HHHHHCCCCEEEEEEEEEEEEEEEECCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
ICKGKVGSSRWLEIHAYWHKLGKMSNATMLFGHIENKIHRIDHMLRIKKIQSPKNKVENK
HHCCCCCCCCEEEHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCHHHCCCC
EGGFNAFIPLLYQKENNYLKVEKSPSAIEILKTIAISRILLNNIPHIKAYWATLGLNLAL
CCCCEEEEEEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHEE
VAQEFGANDLDGTIEIESIQSAAGAKSRHGLEKEDLIFKIKDAGFVAVERDSLYNFIQKF
HHHHHCCCCCCCEEEEHHHHHHCCCHHHCCCCCHHEEEEEECCCEEEEEHHHHHHHHHHC
>Mature Secondary Structure
MDFLEKVLDNQVTESKELVKLYDYDLYTLGEAADHMRQNMHQKIVYFNVNRHLNPSNICT
CCHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHEEEEEECCCCCCHHHHHH
DACKFCAFSAHRKNPNPYEMSLEEIIEKVKNSYNKGIKEVHIVSAHNPNYSYEWYLKVFE
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHH
TIKQEMPNLHLKAMTAAEVHFLSVKFNKPFELVLEDMLKAGVDSMPGGGAEIFDEEVRRK
HHHHHCCCCEEEEEEEEEEEEEEEECCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
ICKGKVGSSRWLEIHAYWHKLGKMSNATMLFGHIENKIHRIDHMLRIKKIQSPKNKVENK
HHCCCCCCCCEEEHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCHHHCCCC
EGGFNAFIPLLYQKENNYLKVEKSPSAIEILKTIAISRILLNNIPHIKAYWATLGLNLAL
CCCCEEEEEEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHEE
VAQEFGANDLDGTIEIESIQSAAGAKSRHGLEKEDLIFKIKDAGFVAVERDSLYNFIQKF
HHHHHCCCCCCCEEEEHHHHHHCCCHHHCCCCCHHEEEEEECCCEEEEEHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11930014 [H]