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The map label for this gene is aspA [H]

Identifier: 108563059

GI number: 108563059

Start: 659305

End: 660711

Strand: Direct

Name: aspA [H]

Synonym: HPAG1_0634

Alternate gene names: 108563059

Gene position: 659305-660711 (Clockwise)

Preceding gene: 108563058

Following gene: 108563060

Centisome position: 41.3

GC content: 43.57

Gene sequence:

>1407_bases
ATGCGTATTGAGCATGATTTCATTGGGCAAATGGAAATTAGCGATGAAGTTTATTATGGGATCCAAACTTTAAGAGCGAG
TGAAAATTTTTTCATCACCAACGACAAGCTTTGCAGTTATCCTGTTTTTATCAAATCTTTCGCTCAAGTCAAAAAAGCGG
CCGCTTTAGCGAACGCGCAATTAGGCTTGATTGATGAAAAGCTTAAAATTGCGATTTGCCATGCGTGCGATTTGTTGGTT
GATGGCAAATACCATGATCAATTCATTGTGGATATGATTCAAGGGGGGGCTGGCACAAGCACGAACATGAACATGAATGA
AGTGATTGCTAATTTGGCTTTGGAATACATGGGGCATCAAAAGGGCGAGTATCAATTTTGCCACCCAAACGACCATGTCA
ATCGCTCTCAATCCACTAATGACGCTTATCCTAGCGCGTTAAAAATCGCTATTTATGAGCGCTTGAGCAATTTAGTCGCT
CCCATGAAGGCTTTAAGGGACGCTTTCGCTCAAAAAGCTAAGGAATTTTCCCATGTGATTAAAATGGGGCGCACCCAGCT
TCAAGACGCTGTGCCTATGACTTTAGGGCAAGAGTTTGAAACTTATGCCTTGATGGTTGATAGGGATATTGAGCAGGTTT
TAGACGCTAGGAATTGGGTAAGAGAGCTTAATTTAGGCGGCACAGCGATTGGCACAGGGATCAATTCGCACCCGGATTAT
CGCAGTTTGATTGAAAAGAAAATCCAAGAAGTAACGGGCCGCCCCTTTGTCATGGCTAATAACTTGATAGAAGCCACTCA
AAGCACGGGAGCGTATGTGCAAGTGAGTGGGGTGTTAAAGCGTATTGCGGTGAAACTCTCTAAAGTTTGTAACGATCTCA
GGTTGCTCAGTTCAGGCCCTAGAGCCGGGCTGAATGAAATCAATTTGCCTAAAATGCAGCCGGGCAGCTCTATTATGCCT
GGTAAAGTCAATCCGGTGATCCCTGAAGTGGTCAATCAGGTGTGCTTTGCGGTGATTGGGAATGATTTGAGCGTGGCGTT
AGCCGCAGAAGGAGGGCAATTGCAGCTCAATGTGTTTGAGCCGGTTATCGCTTACAAGCTATTCCATTCCTTTGTGATTT
TAGGGCGTGCGATTGAAACCTTAACGACTAAATGCGTGGAAGGCATCACGGCTAATGAAAAGATTTGCCACGATTATGTC
TTTAATAGCATTGGCATTGTTACCGCGCTAAACCCTCATATCGGCTATGAAAAATCCGCTATGATCGCTAAAGAAGCTTT
AAAAAGCGATCGCTCTATCTATGACATCGCTTTAGAAAAGAAAATCTTAACTAAAGAGCAACTGGACGATATTTTCAAGC
CAGAAAACATGCTAAGCCCTCACGCTTTCAAAAAGCATAAAGACTGA

Upstream 100 bases:

>100_bases
CAACGCTTTAGGGGCAAAAGTGTTGCGTTTAAAAGAAAAATAATCCAAGATTTGGTTACAATAGCTAGAATATTTTTCAA
TTATTACATAAGGAGCTTTT

Downstream 100 bases:

>100_bases
ACGCTTTGCAACATTCACGCCTTCAAACTTTACGCTCCCTTTATATGGAGCGTCTTTTGGGCGAAACTTACACCAATACC
AACCCTACCACGCCCCAAAA

Product: aspartate ammonia-lyase

Products: NA

Alternate protein names: Aspartase [H]

Number of amino acids: Translated: 468; Mature: 468

Protein sequence:

>468_residues
MRIEHDFIGQMEISDEVYYGIQTLRASENFFITNDKLCSYPVFIKSFAQVKKAAALANAQLGLIDEKLKIAICHACDLLV
DGKYHDQFIVDMIQGGAGTSTNMNMNEVIANLALEYMGHQKGEYQFCHPNDHVNRSQSTNDAYPSALKIAIYERLSNLVA
PMKALRDAFAQKAKEFSHVIKMGRTQLQDAVPMTLGQEFETYALMVDRDIEQVLDARNWVRELNLGGTAIGTGINSHPDY
RSLIEKKIQEVTGRPFVMANNLIEATQSTGAYVQVSGVLKRIAVKLSKVCNDLRLLSSGPRAGLNEINLPKMQPGSSIMP
GKVNPVIPEVVNQVCFAVIGNDLSVALAAEGGQLQLNVFEPVIAYKLFHSFVILGRAIETLTTKCVEGITANEKICHDYV
FNSIGIVTALNPHIGYEKSAMIAKEALKSDRSIYDIALEKKILTKEQLDDIFKPENMLSPHAFKKHKD

Sequences:

>Translated_468_residues
MRIEHDFIGQMEISDEVYYGIQTLRASENFFITNDKLCSYPVFIKSFAQVKKAAALANAQLGLIDEKLKIAICHACDLLV
DGKYHDQFIVDMIQGGAGTSTNMNMNEVIANLALEYMGHQKGEYQFCHPNDHVNRSQSTNDAYPSALKIAIYERLSNLVA
PMKALRDAFAQKAKEFSHVIKMGRTQLQDAVPMTLGQEFETYALMVDRDIEQVLDARNWVRELNLGGTAIGTGINSHPDY
RSLIEKKIQEVTGRPFVMANNLIEATQSTGAYVQVSGVLKRIAVKLSKVCNDLRLLSSGPRAGLNEINLPKMQPGSSIMP
GKVNPVIPEVVNQVCFAVIGNDLSVALAAEGGQLQLNVFEPVIAYKLFHSFVILGRAIETLTTKCVEGITANEKICHDYV
FNSIGIVTALNPHIGYEKSAMIAKEALKSDRSIYDIALEKKILTKEQLDDIFKPENMLSPHAFKKHKD
>Mature_468_residues
MRIEHDFIGQMEISDEVYYGIQTLRASENFFITNDKLCSYPVFIKSFAQVKKAAALANAQLGLIDEKLKIAICHACDLLV
DGKYHDQFIVDMIQGGAGTSTNMNMNEVIANLALEYMGHQKGEYQFCHPNDHVNRSQSTNDAYPSALKIAIYERLSNLVA
PMKALRDAFAQKAKEFSHVIKMGRTQLQDAVPMTLGQEFETYALMVDRDIEQVLDARNWVRELNLGGTAIGTGINSHPDY
RSLIEKKIQEVTGRPFVMANNLIEATQSTGAYVQVSGVLKRIAVKLSKVCNDLRLLSSGPRAGLNEINLPKMQPGSSIMP
GKVNPVIPEVVNQVCFAVIGNDLSVALAAEGGQLQLNVFEPVIAYKLFHSFVILGRAIETLTTKCVEGITANEKICHDYV
FNSIGIVTALNPHIGYEKSAMIAKEALKSDRSIYDIALEKKILTKEQLDDIFKPENMLSPHAFKKHKD

Specific function: Unknown

COG id: COG1027

COG function: function code E; Aspartate ammonia-lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II fumarase/aspartase family. Aspartase subfamily [H]

Homologues:

Organism=Homo sapiens, GI19743875, Length=461, Percent_Identity=43.8177874186551, Blast_Score=376, Evalue=1e-104,
Organism=Escherichia coli, GI87082375, Length=470, Percent_Identity=55.7446808510638, Blast_Score=521, Evalue=1e-149,
Organism=Escherichia coli, GI1787896, Length=459, Percent_Identity=39.4335511982571, Blast_Score=354, Evalue=6e-99,
Organism=Caenorhabditis elegans, GI17553882, Length=462, Percent_Identity=44.3722943722944, Blast_Score=386, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI32565146, Length=326, Percent_Identity=43.8650306748466, Blast_Score=278, Evalue=3e-75,
Organism=Saccharomyces cerevisiae, GI6324993, Length=461, Percent_Identity=41.4316702819957, Blast_Score=367, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24640177, Length=461, Percent_Identity=43.3839479392625, Blast_Score=373, Evalue=1e-103,
Organism=Drosophila melanogaster, GI24640179, Length=461, Percent_Identity=43.3839479392625, Blast_Score=372, Evalue=1e-103,
Organism=Drosophila melanogaster, GI78710009, Length=463, Percent_Identity=43.4125269978402, Blast_Score=369, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24662684, Length=462, Percent_Identity=43.2900432900433, Blast_Score=366, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24583245, Length=462, Percent_Identity=37.4458874458874, Blast_Score=315, Evalue=3e-86,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004708
- InterPro:   IPR003031
- InterPro:   IPR018951
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761 [H]

Pfam domain/function: PF10415 FumaraseC_C; PF00206 Lyase_1 [H]

EC number: =4.3.1.1 [H]

Molecular weight: Translated: 51926; Mature: 51926

Theoretical pI: Translated: 6.83; Mature: 6.83

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIEHDFIGQMEISDEVYYGIQTLRASENFFITNDKLCSYPVFIKSFAQVKKAAALANAQ
CCCCCCCCCCEECCHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHCCH
LGLIDEKLKIAICHACDLLVDGKYHDQFIVDMIQGGAGTSTNMNMNEVIANLALEYMGHQ
HCCCCHHHHHHHHHHHHHEECCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCC
KGEYQFCHPNDHVNRSQSTNDAYPSALKIAIYERLSNLVAPMKALRDAFAQKAKEFSHVI
CCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KMGRTQLQDAVPMTLGQEFETYALMVDRDIEQVLDARNWVRELNLGGTAIGTGINSHPDY
HHHHHHHHHHCCHHHCCCHHHHHEEHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCHH
RSLIEKKIQEVTGRPFVMANNLIEATQSTGAYVQVSGVLKRIAVKLSKVCNDLRLLSSGP
HHHHHHHHHHHCCCCEEEECHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCC
RAGLNEINLPKMQPGSSIMPGKVNPVIPEVVNQVCFAVIGNDLSVALAAEGGQLQLNVFE
CCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCEEEEEHHH
PVIAYKLFHSFVILGRAIETLTTKCVEGITANEKICHDYVFNSIGIVTALNPHIGYEKSA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCEEEECCCCCCCHHH
MIAKEALKSDRSIYDIALEKKILTKEQLDDIFKPENMLSPHAFKKHKD
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCC
>Mature Secondary Structure
MRIEHDFIGQMEISDEVYYGIQTLRASENFFITNDKLCSYPVFIKSFAQVKKAAALANAQ
CCCCCCCCCCEECCHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHCCH
LGLIDEKLKIAICHACDLLVDGKYHDQFIVDMIQGGAGTSTNMNMNEVIANLALEYMGHQ
HCCCCHHHHHHHHHHHHHEECCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCC
KGEYQFCHPNDHVNRSQSTNDAYPSALKIAIYERLSNLVAPMKALRDAFAQKAKEFSHVI
CCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KMGRTQLQDAVPMTLGQEFETYALMVDRDIEQVLDARNWVRELNLGGTAIGTGINSHPDY
HHHHHHHHHHCCHHHCCCHHHHHEEHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCHH
RSLIEKKIQEVTGRPFVMANNLIEATQSTGAYVQVSGVLKRIAVKLSKVCNDLRLLSSGP
HHHHHHHHHHHCCCCEEEECHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCC
RAGLNEINLPKMQPGSSIMPGKVNPVIPEVVNQVCFAVIGNDLSVALAAEGGQLQLNVFE
CCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCEEEEEHHH
PVIAYKLFHSFVILGRAIETLTTKCVEGITANEKICHDYVFNSIGIVTALNPHIGYEKSA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCEEEECCCCCCCHHH
MIAKEALKSDRSIYDIALEKKILTKEQLDDIFKPENMLSPHAFKKHKD
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]