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The map label for this gene is nfnB [C]

Identifier: 108563052

GI number: 108563052

Start: 652717

End: 653274

Strand: Direct

Name: nfnB [C]

Synonym: HPAG1_0627

Alternate gene names: 108563052

Gene position: 652717-653274 (Clockwise)

Preceding gene: 108563051

Following gene: 108563053

Centisome position: 40.89

GC content: 40.86

Gene sequence:

>558_bases
TTGGTTGAAGTGGGGAGATTAGCCCCTTCTTCAATCGGGCTTGAACCATGGAAAATGCTTTTATTAAAGAATGAACGCAT
GAAAGAAGATTTAAAACCGATGGCCTGGGGGGCACTTTTTGGTTTAGAGGGAGCGAGCCATTTTGTCATTTATCTTGCGC
GAAAAGGCGTTACTTATGACAGCGATTACGTTAAGAAAGTGATGCATGAGGTTAAAAAAAGGGATTATGACACTAATTCC
AGGTTCGCTCAAATCATCAAAAATTTCCAAGAGAACGATATGAAACTCAATAGCGAACGATCTTTGTTTGATTGGGCTAG
CAAGCAGACTTATATCCAAATGGCGAACATGATGATGGCAGCGGCCATGTTAGGGATTGATTCTTGCCCGATTGAAGGGT
ATGATCAAGAAAAAGTGGAGGCTTATTTAGAGGAAAAAGGCTATCTGAACACGGCGGAATTTGGGGTATCGGTAATGGCT
TGTTTTGGTTATCGCAACCAAGAAATCACCCCTAAAACCCGTTGGAAAACAGAAGTTATTTATGAAGTGATTGAATAA

Upstream 100 bases:

>100_bases
TTAAAATGGACAGAGAACAAGTGATTGCTTTACAGCACCAACGATTTGCTGCAAAAAATACGATCCTAATCGTCGTATTT
CCCAAAAGGATTGGGAAGCT

Downstream 100 bases:

>100_bases
AAAGCTCTTTTAGCTTTTTGATAACCAATCATAAAAAGCTTGGCGTTAGCCAAGCGCTAATCTCTTAAATGATGCTTGTT
TGTGTTTGATAAAAACAGAA

Product: NAD(P)H-flavin oxidoreductase

Products: NAD(P)(+); @TETR01.txt*5,6,7,8-Tetrahydropteridine!. [C]

Alternate protein names: NA

Number of amino acids: Translated: 185; Mature: 185

Protein sequence:

>185_residues
MVEVGRLAPSSIGLEPWKMLLLKNERMKEDLKPMAWGALFGLEGASHFVIYLARKGVTYDSDYVKKVMHEVKKRDYDTNS
RFAQIIKNFQENDMKLNSERSLFDWASKQTYIQMANMMMAAAMLGIDSCPIEGYDQEKVEAYLEEKGYLNTAEFGVSVMA
CFGYRNQEITPKTRWKTEVIYEVIE

Sequences:

>Translated_185_residues
MVEVGRLAPSSIGLEPWKMLLLKNERMKEDLKPMAWGALFGLEGASHFVIYLARKGVTYDSDYVKKVMHEVKKRDYDTNS
RFAQIIKNFQENDMKLNSERSLFDWASKQTYIQMANMMMAAAMLGIDSCPIEGYDQEKVEAYLEEKGYLNTAEFGVSVMA
CFGYRNQEITPKTRWKTEVIYEVIE
>Mature_185_residues
MVEVGRLAPSSIGLEPWKMLLLKNERMKEDLKPMAWGALFGLEGASHFVIYLARKGVTYDSDYVKKVMHEVKKRDYDTNS
RFAQIIKNFQENDMKLNSERSLFDWASKQTYIQMANMMMAAAMLGIDSCPIEGYDQEKVEAYLEEKGYLNTAEFGVSVMA
CFGYRNQEITPKTRWKTEVIYEVIE

Specific function: Reduction Of A Variety Of Nitroaromatic Compounds Using NADH (And To Lesser Extent Nadph) As Source Of Reducing Equivalents; Two Electrons Are Transferred. Capable Of Reducing Nitrofurazone, Quinones And The Anti-Tumor Agent Cb1954 (5- (Aziridin-1-Yl)-2,4

COG id: COG0778

COG function: function code C; Nitroreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitroreductase family [H]

Homologues:

None

Paralogues:

None

Copy number: 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000415 [H]

Pfam domain/function: PF00881 Nitroreductase [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 21414; Mature: 21414

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
6.5 %Met     (Translated Protein)
7.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
6.5 %Met     (Mature Protein)
7.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVEVGRLAPSSIGLEPWKMLLLKNERMKEDLKPMAWGALFGLEGASHFVIYLARKGVTYD
CCCCCCCCCCCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCC
SDYVKKVMHEVKKRDYDTNSRFAQIIKNFQENDMKLNSERSLFDWASKQTYIQMANMMMA
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCEECCCCHHHHHHHHHHHHHHHHHHHH
AAMLGIDSCPIEGYDQEKVEAYLEEKGYLNTAEFGVSVMACFGYRNQEITPKTRWKTEVI
HHHHCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
YEVIE
HHHHC
>Mature Secondary Structure
MVEVGRLAPSSIGLEPWKMLLLKNERMKEDLKPMAWGALFGLEGASHFVIYLARKGVTYD
CCCCCCCCCCCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCC
SDYVKKVMHEVKKRDYDTNSRFAQIIKNFQENDMKLNSERSLFDWASKQTYIQMANMMMA
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCEECCCCHHHHHHHHHHHHHHHHHHHH
AAMLGIDSCPIEGYDQEKVEAYLEEKGYLNTAEFGVSVMACFGYRNQEITPKTRWKTEVI
HHHHCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
YEVIE
HHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD(P)H; 6,7-Dihydropteridine [C]

Specific reaction: NAD(P)H + 6,7-Dihydropteridine = NAD(P)(+) + @TETR01.txt*5,6,7,8-Tetrahydropteridine!. [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12950922 [H]