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The map label for this gene is folD [H]

Identifier: 108562981

GI number: 108562981

Start: 573086

End: 573964

Strand: Reverse

Name: folD [H]

Synonym: HPAG1_0556

Alternate gene names: 108562981

Gene position: 573964-573086 (Counterclockwise)

Preceding gene: 108562982

Following gene: 108562980

Centisome position: 35.95

GC content: 42.43

Gene sequence:

>879_bases
ATGGGCATGCCAAATAGGGGCGTTGTTTTATTAGACGGGCAAGCGTTAGCTTATGATATAGAAAAAGATTTGAAAAATAA
AATCCAAACAATAACCGCACAAACGCATAAACGCCCCAAACTAGCCGTGATTTTGGTGGGGAAAGATCCCGCTAGTATCA
CTTATGTCAATATGAAGATCAAAGCATGCCAAAGGGTTGGCATGGATTTTGACTTAAAAACCCTCCAAGAGGATATTACT
GAAGCCAAATTGCTGTCCTTGATTAAAGATTACAATACCGATCAAAACATTTCAGGCGTTTTAGTCCAGCTCCCTTTGCC
CAGACACATTGATTCTAAAATGGTTTTAGAAGCCATTGACCCAAGTAAAGATGTGGATGGTTTCCACCCCCTTAATATCG
GCAAGCTCTGCACTCAAAAAGAATCGTTTCTGCCAGCCACCCCTATGGGCGTGATGCGTCTTTTAAAGCATTATCATATT
GAAATCAAGGGCAAGGATGTGGCGATTATTGGGGCGAGCAATATCATTGGAAAACCTTTGAGCATGCTCATGTTAAACGC
TGGGGCTAGCGTGAGCGTGTGCCATATTTTGACTAAAGACATTAGTTTTTACACCCAAAACGCTGATATTGTCTGCGTGG
GCGTGGGTAAGCCTGATTTGATTAAAGCGAGCATGTTAAAAAAAGGGGCTGTAGTGGTGGATATTGGGATCAATCATTTA
AATGATGGGCGTATCGTGGGCGATGTGGATTTTAATAACGCGCAAAAAGTCGCCGGTTTTATCACCCCTGTGCCTAAAGG
CGTGGGGCCTATGACGATCGTCTCGCTTTTAGAAAACACTCTAATCGCTTTTGAAAAACAACAAAGGAAGGGATTTTAA

Upstream 100 bases:

>100_bases
TATCATGGAGCTAATCTTAAAGATTGACAAACTGGGTTTTTATTCCCTTAAACGCATGATTTTTGCTACAATAATAGGAT
TTTAATTATATAAAGGATAA

Downstream 100 bases:

>100_bases
TGAAATTTTTACGCTCTGTTTATGCATTTTGCTCCAGTTGGGTAGGGACGATTGTTATTGTGCTGTTGGTTATCTTTTTT
GTTGCGCAAGCTTTCATCAT

Product: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase [H]

Number of amino acids: Translated: 292; Mature: 291

Protein sequence:

>292_residues
MGMPNRGVVLLDGQALAYDIEKDLKNKIQTITAQTHKRPKLAVILVGKDPASITYVNMKIKACQRVGMDFDLKTLQEDIT
EAKLLSLIKDYNTDQNISGVLVQLPLPRHIDSKMVLEAIDPSKDVDGFHPLNIGKLCTQKESFLPATPMGVMRLLKHYHI
EIKGKDVAIIGASNIIGKPLSMLMLNAGASVSVCHILTKDISFYTQNADIVCVGVGKPDLIKASMLKKGAVVVDIGINHL
NDGRIVGDVDFNNAQKVAGFITPVPKGVGPMTIVSLLENTLIAFEKQQRKGF

Sequences:

>Translated_292_residues
MGMPNRGVVLLDGQALAYDIEKDLKNKIQTITAQTHKRPKLAVILVGKDPASITYVNMKIKACQRVGMDFDLKTLQEDIT
EAKLLSLIKDYNTDQNISGVLVQLPLPRHIDSKMVLEAIDPSKDVDGFHPLNIGKLCTQKESFLPATPMGVMRLLKHYHI
EIKGKDVAIIGASNIIGKPLSMLMLNAGASVSVCHILTKDISFYTQNADIVCVGVGKPDLIKASMLKKGAVVVDIGINHL
NDGRIVGDVDFNNAQKVAGFITPVPKGVGPMTIVSLLENTLIAFEKQQRKGF
>Mature_291_residues
GMPNRGVVLLDGQALAYDIEKDLKNKIQTITAQTHKRPKLAVILVGKDPASITYVNMKIKACQRVGMDFDLKTLQEDITE
AKLLSLIKDYNTDQNISGVLVQLPLPRHIDSKMVLEAIDPSKDVDGFHPLNIGKLCTQKESFLPATPMGVMRLLKHYHIE
IKGKDVAIIGASNIIGKPLSMLMLNAGASVSVCHILTKDISFYTQNADIVCVGVGKPDLIKASMLKKGAVVVDIGINHLN
DGRIVGDVDFNNAQKVAGFITPVPKGVGPMTIVSLLENTLIAFEKQQRKGF

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate [H]

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family [H]

Homologues:

Organism=Homo sapiens, GI222418558, Length=293, Percent_Identity=41.6382252559727, Blast_Score=238, Evalue=5e-63,
Organism=Homo sapiens, GI94721354, Length=296, Percent_Identity=42.5675675675676, Blast_Score=229, Evalue=2e-60,
Organism=Homo sapiens, GI222136639, Length=283, Percent_Identity=36.7491166077738, Blast_Score=191, Evalue=7e-49,
Organism=Homo sapiens, GI36796743, Length=206, Percent_Identity=26.6990291262136, Blast_Score=75, Evalue=8e-14,
Organism=Escherichia coli, GI1786741, Length=274, Percent_Identity=48.1751824817518, Blast_Score=271, Evalue=3e-74,
Organism=Caenorhabditis elegans, GI17568735, Length=260, Percent_Identity=38.4615384615385, Blast_Score=172, Evalue=1e-43,
Organism=Saccharomyces cerevisiae, GI6319558, Length=287, Percent_Identity=42.5087108013937, Blast_Score=239, Evalue=4e-64,
Organism=Saccharomyces cerevisiae, GI6321643, Length=298, Percent_Identity=38.9261744966443, Blast_Score=213, Evalue=2e-56,
Organism=Saccharomyces cerevisiae, GI6322933, Length=261, Percent_Identity=22.2222222222222, Blast_Score=68, Evalue=1e-12,
Organism=Drosophila melanogaster, GI17136818, Length=293, Percent_Identity=41.9795221843003, Blast_Score=244, Evalue=4e-65,
Organism=Drosophila melanogaster, GI17136816, Length=293, Percent_Identity=41.9795221843003, Blast_Score=244, Evalue=5e-65,
Organism=Drosophila melanogaster, GI62472483, Length=285, Percent_Identity=40, Blast_Score=209, Evalue=2e-54,
Organism=Drosophila melanogaster, GI45551871, Length=285, Percent_Identity=40, Blast_Score=209, Evalue=2e-54,
Organism=Drosophila melanogaster, GI24645718, Length=285, Percent_Identity=40, Blast_Score=209, Evalue=2e-54,
Organism=Drosophila melanogaster, GI17137370, Length=285, Percent_Identity=40, Blast_Score=209, Evalue=2e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631 [H]

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C [H]

EC number: =1.5.1.5; =3.5.4.9 [H]

Molecular weight: Translated: 31805; Mature: 31674

Theoretical pI: Translated: 9.34; Mature: 9.34

Prosite motif: PS00766 THF_DHG_CYH_1 ; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGMPNRGVVLLDGQALAYDIEKDLKNKIQTITAQTHKRPKLAVILVGKDPASITYVNMKI
CCCCCCCEEEECCCEEEEHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEEEEEEE
KACQRVGMDFDLKTLQEDITEAKLLSLIKDYNTDQNISGVLVQLPLPRHIDSKMVLEAID
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCHHHEEECC
PSKDVDGFHPLNIGKLCTQKESFLPATPMGVMRLLKHYHIEIKGKDVAIIGASNIIGKPL
CCCCCCCCCCCCHHHHHCCCCCCCCCCCHHHHHHHHHHEEEECCCEEEEEECCCCCCCCH
SMLMLNAGASVSVCHILTKDISFYTQNADIVCVGVGKPDLIKASMLKKGAVVVDIGINHL
HHHEECCCCCEEEHHHHHHHHHEEECCCCEEEEECCCCCHHHHHHHHCCCEEEEECCCCC
NDGRIVGDVDFNNAQKVAGFITPVPKGVGPMTIVSLLENTLIAFEKQQRKGF
CCCEEEEEECCCCHHHHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
GMPNRGVVLLDGQALAYDIEKDLKNKIQTITAQTHKRPKLAVILVGKDPASITYVNMKI
CCCCCCEEEECCCEEEEHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEEEEEEE
KACQRVGMDFDLKTLQEDITEAKLLSLIKDYNTDQNISGVLVQLPLPRHIDSKMVLEAID
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCHHHEEECC
PSKDVDGFHPLNIGKLCTQKESFLPATPMGVMRLLKHYHIEIKGKDVAIIGASNIIGKPL
CCCCCCCCCCCCHHHHHCCCCCCCCCCCHHHHHHHHHHEEEECCCEEEEEECCCCCCCCH
SMLMLNAGASVSVCHILTKDISFYTQNADIVCVGVGKPDLIKASMLKKGAVVVDIGINHL
HHHEECCCCCEEEHHHHHHHHHEEECCCCEEEEECCCCCHHHHHHHHCCCEEEEECCCCC
NDGRIVGDVDFNNAQKVAGFITPVPKGVGPMTIVSLLENTLIAFEKQQRKGF
CCCEEEEEECCCCHHHHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA