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The map label for this gene is prfB [H]

Identifier: 108562592

GI number: 108562592

Start: 172822

End: 173913

Strand: Reverse

Name: prfB [H]

Synonym: HPAG1_0167

Alternate gene names: 108562592

Gene position: 173913-172822 (Counterclockwise)

Preceding gene: 108562593

Following gene: 108562591

Centisome position: 10.89

GC content: 42.03

Gene sequence:

>1092_bases
GTGGATAACTACACCTATAGCGAATTGTTAAAAAGCTTGCAAAACAAATGCGATAATATCGCTTTAATCATCAAGCCTGA
AAAGATCAAACAAGAATTAGAACGCATTGAAAAAGAGCAAGAAGACCCTAATTTTTGGCAAGATGTCTTAAAAGCCAGAG
ACACCAATAAAGAAAAAGTGCGCCTAAACCGCTTGCTAGAAACCTATCAAAAAACCAAAAATTCTCTAGATGAAAGCGTA
GAATTGTTTGAACTCGCTCAAAACGATAACGATGAGGTTACTTTATCCTTGCTCTATGAAGAGGCTCCTACTTTAGAACA
CAGCGTCCAAAAAGTGGAAATTGAAATCATGCTAAGCGGTGAAAACGACGCTTCAAACGCCATTATCACCATTCAGCCTG
GAGCGGGGGGGACTGAAAGTCAGGATTGGGCGAGCATTTTGTATCGCATGTATTTGAGATGGGCGGAAAGAAGGGGCTTT
AAAAGCGAGATTTTAGATTACCAAGATGGCGAAGAAGCGGGCATTAAAGGGGTCGCCTTTATCATTAAGGGCGAAAACGC
TTATGGCTATTTGAAAAATGAAAATGGGGTGCATAGGCTTGTAAGGATTTCGCCCTTTGATGCGAACGCCAAACGGCACA
CGAGTTTTGCGAGCGTGCAAATTAGCCCTGAATTAGACGATGATATTGATATAGAAATTGATGAAAAAGATGTCCGTTAT
GATTATTACAGAGCCAGTGGGGCAGGCGGTCAGCATGTCAATAAAACAGAAAGCGCGGTCAGGATCACGCATTTCCCTAC
CGGTATTGTGGTGCAATGCCAAAACGACAGGAGCCAGCATAAAAACAAAGCGAGCGCGTTAAAAATGCTTAAATCCAAGC
TTTATGAATTGGAATTAGAAAAGCAGCAAAGCAGCGCCAAAAATGAAGAAAAAAGCGAGATTGGTTGGGGGCATCAAATA
AGAAGCTATGTCCTAGCCCCCTACCAGCAAGTCAAAGACGCACGCTCCAATATTGCTTATAGCAATGTGGAAGCGATACT
AGATGGCGATATTGATGCGATTTTAGAGGGCGTTTTGATTGCTAAAGCTTAA

Upstream 100 bases:

>100_bases
GGTTCAGGGCGAAATTGAAATTTTAAGGTTTGAAAATTAAAATGGCTTTTAACAATGCTATAATGATAAGTTCAAAACCC
CACTCATAAAGGTTCTGTTT

Downstream 100 bases:

>100_bases
GTGCGCTAAAATGACAGCTAATCAGTTATGGTTACTATAAAACGCTTTTTAAAACCAATTGGCAAGGAACAACAATGACA
CAAGAAGAATTAGACGCTTT

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 363; Mature: 363

Protein sequence:

>363_residues
MDNYTYSELLKSLQNKCDNIALIIKPEKIKQELERIEKEQEDPNFWQDVLKARDTNKEKVRLNRLLETYQKTKNSLDESV
ELFELAQNDNDEVTLSLLYEEAPTLEHSVQKVEIEIMLSGENDASNAIITIQPGAGGTESQDWASILYRMYLRWAERRGF
KSEILDYQDGEEAGIKGVAFIIKGENAYGYLKNENGVHRLVRISPFDANAKRHTSFASVQISPELDDDIDIEIDEKDVRY
DYYRASGAGGQHVNKTESAVRITHFPTGIVVQCQNDRSQHKNKASALKMLKSKLYELELEKQQSSAKNEEKSEIGWGHQI
RSYVLAPYQQVKDARSNIAYSNVEAILDGDIDAILEGVLIAKA

Sequences:

>Translated_363_residues
MDNYTYSELLKSLQNKCDNIALIIKPEKIKQELERIEKEQEDPNFWQDVLKARDTNKEKVRLNRLLETYQKTKNSLDESV
ELFELAQNDNDEVTLSLLYEEAPTLEHSVQKVEIEIMLSGENDASNAIITIQPGAGGTESQDWASILYRMYLRWAERRGF
KSEILDYQDGEEAGIKGVAFIIKGENAYGYLKNENGVHRLVRISPFDANAKRHTSFASVQISPELDDDIDIEIDEKDVRY
DYYRASGAGGQHVNKTESAVRITHFPTGIVVQCQNDRSQHKNKASALKMLKSKLYELELEKQQSSAKNEEKSEIGWGHQI
RSYVLAPYQQVKDARSNIAYSNVEAILDGDIDAILEGVLIAKA
>Mature_363_residues
MDNYTYSELLKSLQNKCDNIALIIKPEKIKQELERIEKEQEDPNFWQDVLKARDTNKEKVRLNRLLETYQKTKNSLDESV
ELFELAQNDNDEVTLSLLYEEAPTLEHSVQKVEIEIMLSGENDASNAIITIQPGAGGTESQDWASILYRMYLRWAERRGF
KSEILDYQDGEEAGIKGVAFIIKGENAYGYLKNENGVHRLVRISPFDANAKRHTSFASVQISPELDDDIDIEIDEKDVRY
DYYRASGAGGQHVNKTESAVRITHFPTGIVVQCQNDRSQHKNKASALKMLKSKLYELELEKQQSSAKNEEKSEIGWGHQI
RSYVLAPYQQVKDARSNIAYSNVEAILDGDIDAILEGVLIAKA

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=277, Percent_Identity=36.8231046931408, Blast_Score=174, Evalue=2e-43,
Organism=Homo sapiens, GI34577120, Length=324, Percent_Identity=32.0987654320988, Blast_Score=153, Evalue=2e-37,
Organism=Homo sapiens, GI166795305, Length=191, Percent_Identity=36.6492146596859, Blast_Score=119, Evalue=3e-27,
Organism=Escherichia coli, GI2367172, Length=354, Percent_Identity=49.7175141242938, Blast_Score=352, Evalue=2e-98,
Organism=Escherichia coli, GI1787462, Length=241, Percent_Identity=39.8340248962656, Blast_Score=195, Evalue=4e-51,
Organism=Caenorhabditis elegans, GI17542784, Length=280, Percent_Identity=29.6428571428571, Blast_Score=106, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6321295, Length=327, Percent_Identity=31.1926605504587, Blast_Score=142, Evalue=8e-35,
Organism=Drosophila melanogaster, GI19921226, Length=211, Percent_Identity=37.4407582938389, Blast_Score=149, Evalue=2e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 41325; Mature: 41325

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDNYTYSELLKSLQNKCDNIALIIKPEKIKQELERIEKEQEDPNFWQDVLKARDTNKEKV
CCCCCHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHH
RLNRLLETYQKTKNSLDESVELFELAQNDNDEVTLSLLYEEAPTLEHSVQKVEIEIMLSG
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHEEEEEEEEEEC
ENDASNAIITIQPGAGGTESQDWASILYRMYLRWAERRGFKSEILDYQDGEEAGIKGVAF
CCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCEEEE
IIKGENAYGYLKNENGVHRLVRISPFDANAKRHTSFASVQISPELDDDIDIEIDEKDVRY
EEECCCCEEEEECCCCCEEEEEECCCCCCCCCCCCEEEEEECCCCCCCCEEEECCCCCEE
DYYRASGAGGQHVNKTESAVRITHFPTGIVVQCQNDRSQHKNKASALKMLKSKLYELELE
EEEECCCCCCCCCCCCCCEEEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHH
KQQSSAKNEEKSEIGWGHQIRSYVLAPYQQVKDARSNIAYSNVEAILDGDIDAILEGVLI
HHHHHCCCCHHHHCCCHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHH
AKA
CCC
>Mature Secondary Structure
MDNYTYSELLKSLQNKCDNIALIIKPEKIKQELERIEKEQEDPNFWQDVLKARDTNKEKV
CCCCCHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHH
RLNRLLETYQKTKNSLDESVELFELAQNDNDEVTLSLLYEEAPTLEHSVQKVEIEIMLSG
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHEEEEEEEEEEC
ENDASNAIITIQPGAGGTESQDWASILYRMYLRWAERRGFKSEILDYQDGEEAGIKGVAF
CCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCEEEE
IIKGENAYGYLKNENGVHRLVRISPFDANAKRHTSFASVQISPELDDDIDIEIDEKDVRY
EEECCCCEEEEECCCCCEEEEEECCCCCCCCCCCCEEEEEECCCCCCCCEEEECCCCCEE
DYYRASGAGGQHVNKTESAVRITHFPTGIVVQCQNDRSQHKNKASALKMLKSKLYELELE
EEEECCCCCCCCCCCCCCEEEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHH
KQQSSAKNEEKSEIGWGHQIRSYVLAPYQQVKDARSNIAYSNVEAILDGDIDAILEGVLI
HHHHHCCCCHHHHCCCHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHH
AKA
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA