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The map label for this gene is cbdA [H]

Identifier: 107022858

GI number: 107022858

Start: 1435799

End: 1437157

Strand: Reverse

Name: cbdA [H]

Synonym: Bcen_1306

Alternate gene names: 107022858

Gene position: 1437157-1435799 (Counterclockwise)

Preceding gene: 107022859

Following gene: 107022857

Centisome position: 43.62

GC content: 63.06

Gene sequence:

>1359_bases
ATGTCCGCCACGATCGACCACACCAACGACCTGGATCATCTGCTGGAAACCGCCGTGCAGGATGACAAGGAGGCCGGCAT
CTTCCGCTGCCGCCGCGACATCTTCACGAACGCGGAGCTGTACGAGCTCGAGATGAAGCACATCTTCGAGGGCAACTGGG
TGTACCTGGCGCACGAAAGCCAGATTCCCGACAACAACGATTACTACACGACATGGATCGGCCGCCAGCCGATCGTCATC
ACGCGCGACAAGACCGGCGAGCTGCATGCGGTCATCAATGCGTGTGCGCACAAGGGCGCGATGCTGTGCCGCCGCAAGCA
CGGCAACAAGGGTAGCTTCACGTGCCCGTTCCACGGCTGGACCTTCTCGAACACCGGCAAGCTGCTGAAGGTGAAGGACG
AGAAGACGACCGAGTATCCGGTGCAGTTCAACACGCACGGCTCGCACGACCTGAAGAAGGTCGCGCGCTTCGCGAACTAT
CGCGGCTTCCTGTTCGGCAGCCTCAACGCCGACGTGCTGCCGCTCGAGGATTACCTCGGCGAAGCGCGCGTGATCATCGA
CCAGATCGTCGACCAGGCGCCGAACGGGCTCGAAGTGCTGCGCGGCAATTCGTCCTACATCTACGAAGGCAACTGGAAGA
TGCAGATGGAGAACGGTTGCGACGGCTATCACGTGAGCACCGTGCACTGGAACTATGCGGCGACGATGGGCCGCCGCAAG
GAAGACGGCACCAAGGCGGTCGACGCGAACAGCTGGAGCAAGTCGGTCGCGGGCGTGTACGGCTTCGACCACGGCCACAT
CCTGCTGTGGACCCGGACGATGAACCCGGAGGTGCGGCCCGTGTACCGGCATCGTGACGAGATCAAGGCGCGCGTGGGCG
ACGTGCAGGCCGATTTCATCGTGAACCAGACGCGCAACCTGTGCGTGTACCCGAACGTGTTCCTGATGGACCAGTTCAGC
ACGCAGATTCGTGTGGTGCGGCCGCTCGCGGTCGACAAGACGGAAGTCAGCATCTTCTGCTTCGCGCCGAAGGGCGAGAG
CGAGGCCGACCGCACGACGCGGATTCGCCAGTACGAGGATTTCTTCAACGTGACCGGCATGGGCACGGCCGACGATCTCG
AGGAGTTTCGCGCGTGCCAGGCCGGCTATGCAGGCATCACGGCGATGTGGAACGACCTGTCGCGCGGTGCGCCGCTGTGG
GTCGACGGGCCGGACGAGAACGCGAAAAAGATGGGGCTGAACCCGCGCATTTCGGGCGAGCGCAGCGAGGACGAAGGGCT
GTTCGTGTGCCAGCACGAACACTGGGTGAACGTGATGCGCGATGCGCTGAAGAAGGAACGCGGGGAGGTGGCGGCATGA

Upstream 100 bases:

>100_bases
GCGCGCGAGCGCCACCGCTGCCGGCCGGTCACCCCGAGATTCAACCGATACCGATGCGAGCGTGCTTGCAGGACGCGGCA
CGCGCATGGGAGGAGCCATC

Downstream 100 bases:

>100_bases
GCTTCGATTACCGGACGATTTGCGCGGCGCTGTATCGCGAGGCGCGCCTGCTCGACGATCGCGAGTGGGACGCGTGACTG
ACCTGCTACACGGAAGACGT

Product: Rieske (2Fe-2S) region

Products: NA

Alternate protein names: 2-chlorobenzoate 1,2-dioxygenase [H]

Number of amino acids: Translated: 452; Mature: 451

Protein sequence:

>452_residues
MSATIDHTNDLDHLLETAVQDDKEAGIFRCRRDIFTNAELYELEMKHIFEGNWVYLAHESQIPDNNDYYTTWIGRQPIVI
TRDKTGELHAVINACAHKGAMLCRRKHGNKGSFTCPFHGWTFSNTGKLLKVKDEKTTEYPVQFNTHGSHDLKKVARFANY
RGFLFGSLNADVLPLEDYLGEARVIIDQIVDQAPNGLEVLRGNSSYIYEGNWKMQMENGCDGYHVSTVHWNYAATMGRRK
EDGTKAVDANSWSKSVAGVYGFDHGHILLWTRTMNPEVRPVYRHRDEIKARVGDVQADFIVNQTRNLCVYPNVFLMDQFS
TQIRVVRPLAVDKTEVSIFCFAPKGESEADRTTRIRQYEDFFNVTGMGTADDLEEFRACQAGYAGITAMWNDLSRGAPLW
VDGPDENAKKMGLNPRISGERSEDEGLFVCQHEHWVNVMRDALKKERGEVAA

Sequences:

>Translated_452_residues
MSATIDHTNDLDHLLETAVQDDKEAGIFRCRRDIFTNAELYELEMKHIFEGNWVYLAHESQIPDNNDYYTTWIGRQPIVI
TRDKTGELHAVINACAHKGAMLCRRKHGNKGSFTCPFHGWTFSNTGKLLKVKDEKTTEYPVQFNTHGSHDLKKVARFANY
RGFLFGSLNADVLPLEDYLGEARVIIDQIVDQAPNGLEVLRGNSSYIYEGNWKMQMENGCDGYHVSTVHWNYAATMGRRK
EDGTKAVDANSWSKSVAGVYGFDHGHILLWTRTMNPEVRPVYRHRDEIKARVGDVQADFIVNQTRNLCVYPNVFLMDQFS
TQIRVVRPLAVDKTEVSIFCFAPKGESEADRTTRIRQYEDFFNVTGMGTADDLEEFRACQAGYAGITAMWNDLSRGAPLW
VDGPDENAKKMGLNPRISGERSEDEGLFVCQHEHWVNVMRDALKKERGEVAA
>Mature_451_residues
SATIDHTNDLDHLLETAVQDDKEAGIFRCRRDIFTNAELYELEMKHIFEGNWVYLAHESQIPDNNDYYTTWIGRQPIVIT
RDKTGELHAVINACAHKGAMLCRRKHGNKGSFTCPFHGWTFSNTGKLLKVKDEKTTEYPVQFNTHGSHDLKKVARFANYR
GFLFGSLNADVLPLEDYLGEARVIIDQIVDQAPNGLEVLRGNSSYIYEGNWKMQMENGCDGYHVSTVHWNYAATMGRRKE
DGTKAVDANSWSKSVAGVYGFDHGHILLWTRTMNPEVRPVYRHRDEIKARVGDVQADFIVNQTRNLCVYPNVFLMDQFST
QIRVVRPLAVDKTEVSIFCFAPKGESEADRTTRIRQYEDFFNVTGMGTADDLEEFRACQAGYAGITAMWNDLSRGAPLWV
DGPDENAKKMGLNPRISGERSEDEGLFVCQHEHWVNVMRDALKKERGEVAA

Specific function: Component of 2-halobenzoate dioxygenase multicomponent enzyme system which catalyzes the incorporation of both atoms of molecular oxygen into 2-halobenzoate to form catechol [H]

COG id: COG4638

COG function: function code PR; Phenylpropionate dioxygenase and related ring-hydroxylating dioxygenases, large terminal subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Rieske domain [H]

Homologues:

Organism=Escherichia coli, GI1788888, Length=362, Percent_Identity=28.1767955801105, Blast_Score=152, Evalue=3e-38,
Organism=Escherichia coli, GI1788103, Length=224, Percent_Identity=25, Blast_Score=82, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017941
- InterPro:   IPR015881
- InterPro:   IPR015879
- InterPro:   IPR001663 [H]

Pfam domain/function: PF00355 Rieske; PF00848 Ring_hydroxyl_A [H]

EC number: =1.14.12.13 [H]

Molecular weight: Translated: 51463; Mature: 51332

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSATIDHTNDLDHLLETAVQDDKEAGIFRCRRDIFTNAELYELEMKHIFEGNWVYLAHES
CCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEEEEEHHEECCCEEEEEECC
QIPDNNDYYTTWIGRQPIVITRDKTGELHAVINACAHKGAMLCRRKHGNKGSFTCPFHGW
CCCCCCCEEEEEECCCCEEEECCCCCCHHHHHHHHHHCCHHHHHHCCCCCCCEEECCCCC
TFSNTGKLLKVKDEKTTEYPVQFNTHGSHDLKKVARFANYRGFLFGSLNADVLPLEDYLG
EECCCCCEEEEECCCCCCCCEEECCCCCHHHHHHHHHHCCCEEEEECCCCCEEEHHHHHC
EARVIIDQIVDQAPNGLEVLRGNSSYIYEGNWKMQMENGCDGYHVSTVHWNYAATMGRRK
HHHHHHHHHHHCCCCCEEEEECCCCEEEECCEEEEECCCCCCEEEEEEEEEHHHHCCCCC
EDGTKAVDANSWSKSVAGVYGFDHGHILLWTRTMNPEVRPVYRHRDEIKARVGDVQADFI
CCCCCEECCCCCCCCCCEEEECCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCEEEEE
VNQTRNLCVYPNVFLMDQFSTQIRVVRPLAVDKTEVSIFCFAPKGESEADRTTRIRQYED
EECCCCEEECCCEEEEECCCCEEEEEEEEEECCCEEEEEEECCCCCCCCHHHHHHHHHHH
FFNVTGMGTADDLEEFRACQAGYAGITAMWNDLSRGAPLWVDGPDENAKKMGLNPRISGE
HHCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEECCCCCHHHCCCCCCCCCC
RSEDEGLFVCQHEHWVNVMRDALKKERGEVAA
CCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SATIDHTNDLDHLLETAVQDDKEAGIFRCRRDIFTNAELYELEMKHIFEGNWVYLAHES
CCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEEEEEHHEECCCEEEEEECC
QIPDNNDYYTTWIGRQPIVITRDKTGELHAVINACAHKGAMLCRRKHGNKGSFTCPFHGW
CCCCCCCEEEEEECCCCEEEECCCCCCHHHHHHHHHHCCHHHHHHCCCCCCCEEECCCCC
TFSNTGKLLKVKDEKTTEYPVQFNTHGSHDLKKVARFANYRGFLFGSLNADVLPLEDYLG
EECCCCCEEEEECCCCCCCCEEECCCCCHHHHHHHHHHCCCEEEEECCCCCEEEHHHHHC
EARVIIDQIVDQAPNGLEVLRGNSSYIYEGNWKMQMENGCDGYHVSTVHWNYAATMGRRK
HHHHHHHHHHHCCCCCEEEEECCCCEEEECCEEEEECCCCCCEEEEEEEEEHHHHCCCCC
EDGTKAVDANSWSKSVAGVYGFDHGHILLWTRTMNPEVRPVYRHRDEIKARVGDVQADFI
CCCCCEECCCCCCCCCCEEEECCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCEEEEE
VNQTRNLCVYPNVFLMDQFSTQIRVVRPLAVDKTEVSIFCFAPKGESEADRTTRIRQYED
EECCCCEEECCCEEEEECCCCEEEEEEEEEECCCEEEEEEECCCCCCCCHHHHHHHHHHH
FFNVTGMGTADDLEEFRACQAGYAGITAMWNDLSRGAPLWVDGPDENAKKMGLNPRISGE
HHCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEECCCCCHHHCCCCCCCCCC
RSEDEGLFVCQHEHWVNVMRDALKKERGEVAA
CCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7530709; 1370284 [H]