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The map label for this gene is proP [H]

Identifier: 107022356

GI number: 107022356

Start: 865437

End: 866795

Strand: Reverse

Name: proP [H]

Synonym: Bcen_0800

Alternate gene names: 107022356

Gene position: 866795-865437 (Counterclockwise)

Preceding gene: 107022357

Following gene: 107022353

Centisome position: 26.31

GC content: 68.06

Gene sequence:

>1359_bases
ATGGCCATCGGCAATGCGATGGAATGGTTCGACTTCGGCGTCTACAGCTATATCGCCGTCACGCTCGGCAAGGTGTTCTT
TCCGTCCAGCAGCCCGTCCGCGCAGTTGCTGGCCACCTTCGGCACGTTCGCGGCCGCGTTCCTCGTGCGCCCGCTCGGCG
GCATGGTGTTCGGCCCGCTCGGCGACCGCATCGGCCGCCAGCGCGTGCTCGCCGCCACGATGATCATGATGGCCGTCGGC
ACCTTCGCGATCGGCCTGATCCCGAGCTATGCGTCGATCGGCATCATGGCCCCGATCCTGCTGCTCGTCGCCCGCCTCGT
GCAGGGCTTCTCGACCGGCGGCGAGTACGGCGGCGCCGCCACTTTCATCGCCGAGTTCTCGACCGACAAGCGCCGCGGCT
TCATGGGCAGCTTCCTCGAATTCGGCACGCTGATCGGCTATGTGCTGGGCGCGGGCGTCGTCGCACTGCTCACCGCGTCG
CTGTCGCAGGAAGCGCTGCTGTCGTGGGGCTGGCGCGTGCCGTTCCTGATCGCCGGGCCGCTCGGCCTGATCGGTCTGTA
CATCCGGATGAAGCTCGAGGAAACGCCGGCGTTCAAGCGCCAGGCCGAAGAGCGCGAAGCGCAGGACAAGGCCGTGCCGA
AGGCGCGCTTTCGCGAGACGCTGCTGCGCAACTGGCGTGCGCTGCTGCTGTGCGTCGGCCTCGTGCTGATCTTCAACGTG
ACCGACTACATGGTGCTGTCTTACCTGCCGAGCTTCATGTCGTCGACGCTGCACTTCGACGAATCGCACAGCCTCGTGCT
GGTGCTGATCGTGATGGTGCTGATGATGCCGATGACGCTGTACGCCGGCCGCCTGTCGGACAAGGTCGGCCGCAAGCCGG
TGATGCTCGCCGGCTGCGTCGGCCTGCTGGTGCTGTCGATCCCGTCGCTGATGCTGATCCACACGGGCACCACGGCGTCG
GTGTTCGGCGGCCTGCTGATCCTCGGCGTGCTGCTGTCGTGCTTCACCGGCGTGATGCCGTCGGCGCTGCCCGCGCTGTT
CCCGACCGAGATCCGCTACGGCGCGCTCGCGATCGGCTTCAACGTGTCGGTGTCGCTGTTCGGCGGCACGACGCCGCTGG
TGACCGCGTGGCTCGTCGACGTGACGCACAACCTGATGATGCCCGCGTACTACATGATGGGCGCCGCGCTGATCGGCATC
GTGTCGGTCGTCGCGCTCGCCGAAACCGCGCGCCAGCCGCTGAAGGGCTCGCCGCCGGCCGTCGCGACGCGCCGCGAAGC
GCACCAGCTGGCGCGCCAGTTGCGCGAGGACGACGACGAATCGGAGGTCTACGGCGTCGCGACGCCCGCCCGCGCGTAA

Upstream 100 bases:

>100_bases
CGCTGTCTCTTCCCCCGCTTCGTCGTCCGGCGAAGCCCCGCTCGCCGCGGACGACATCACCGTCGTCGACCAGAGCCTGC
TCAAGCGCGCCGTCAGCGCA

Downstream 100 bases:

>100_bases
GCGTCGCGCAATGAAAAGCCCCGGCCGAAGCCGGGGCCCGATCGAACCCGATCTGCCCGACCGGCTTGCGCCGGCCGGGC
TTTTTTCATTCAGTCGGCAC

Product: general substrate transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 452; Mature: 451

Protein sequence:

>452_residues
MAIGNAMEWFDFGVYSYIAVTLGKVFFPSSSPSAQLLATFGTFAAAFLVRPLGGMVFGPLGDRIGRQRVLAATMIMMAVG
TFAIGLIPSYASIGIMAPILLLVARLVQGFSTGGEYGGAATFIAEFSTDKRRGFMGSFLEFGTLIGYVLGAGVVALLTAS
LSQEALLSWGWRVPFLIAGPLGLIGLYIRMKLEETPAFKRQAEEREAQDKAVPKARFRETLLRNWRALLLCVGLVLIFNV
TDYMVLSYLPSFMSSTLHFDESHSLVLVLIVMVLMMPMTLYAGRLSDKVGRKPVMLAGCVGLLVLSIPSLMLIHTGTTAS
VFGGLLILGVLLSCFTGVMPSALPALFPTEIRYGALAIGFNVSVSLFGGTTPLVTAWLVDVTHNLMMPAYYMMGAALIGI
VSVVALAETARQPLKGSPPAVATRREAHQLARQLREDDDESEVYGVATPARA

Sequences:

>Translated_452_residues
MAIGNAMEWFDFGVYSYIAVTLGKVFFPSSSPSAQLLATFGTFAAAFLVRPLGGMVFGPLGDRIGRQRVLAATMIMMAVG
TFAIGLIPSYASIGIMAPILLLVARLVQGFSTGGEYGGAATFIAEFSTDKRRGFMGSFLEFGTLIGYVLGAGVVALLTAS
LSQEALLSWGWRVPFLIAGPLGLIGLYIRMKLEETPAFKRQAEEREAQDKAVPKARFRETLLRNWRALLLCVGLVLIFNV
TDYMVLSYLPSFMSSTLHFDESHSLVLVLIVMVLMMPMTLYAGRLSDKVGRKPVMLAGCVGLLVLSIPSLMLIHTGTTAS
VFGGLLILGVLLSCFTGVMPSALPALFPTEIRYGALAIGFNVSVSLFGGTTPLVTAWLVDVTHNLMMPAYYMMGAALIGI
VSVVALAETARQPLKGSPPAVATRREAHQLARQLREDDDESEVYGVATPARA
>Mature_451_residues
AIGNAMEWFDFGVYSYIAVTLGKVFFPSSSPSAQLLATFGTFAAAFLVRPLGGMVFGPLGDRIGRQRVLAATMIMMAVGT
FAIGLIPSYASIGIMAPILLLVARLVQGFSTGGEYGGAATFIAEFSTDKRRGFMGSFLEFGTLIGYVLGAGVVALLTASL
SQEALLSWGWRVPFLIAGPLGLIGLYIRMKLEETPAFKRQAEEREAQDKAVPKARFRETLLRNWRALLLCVGLVLIFNVT
DYMVLSYLPSFMSSTLHFDESHSLVLVLIVMVLMMPMTLYAGRLSDKVGRKPVMLAGCVGLLVLSIPSLMLIHTGTTASV
FGGLLILGVLLSCFTGVMPSALPALFPTEIRYGALAIGFNVSVSLFGGTTPLVTAWLVDVTHNLMMPAYYMMGAALIGIV
SVVALAETARQPLKGSPPAVATRREAHQLARQLREDDDESEVYGVATPARA

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=452, Percent_Identity=51.3274336283186, Blast_Score=494, Evalue=1e-141,
Organism=Escherichia coli, GI1788292, Length=414, Percent_Identity=31.8840579710145, Blast_Score=207, Evalue=9e-55,
Organism=Escherichia coli, GI1788942, Length=378, Percent_Identity=35.4497354497354, Blast_Score=201, Evalue=1e-52,
Organism=Escherichia coli, GI1789941, Length=418, Percent_Identity=33.7320574162679, Blast_Score=191, Evalue=1e-49,
Organism=Escherichia coli, GI87082231, Length=423, Percent_Identity=22.9314420803783, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI87082404, Length=357, Percent_Identity=23.5294117647059, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 48502; Mature: 48371

Theoretical pI: Translated: 8.98; Mature: 8.98

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1 ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHCCCCCCCEEECCCCCCC
>Mature Secondary Structure 
AIGNAMEWFDFGVYSYIAVTLGKVFFPSSSPSAQLLATFGTFAAAFLVRPLGGMVFGPL
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEECCH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HHHHHHHHHHHHHHHCCCCCCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]