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The map label for this gene is glcF [H]

Identifier: 107021808

GI number: 107021808

Start: 266493

End: 267719

Strand: Reverse

Name: glcF [H]

Synonym: Bcen_0248

Alternate gene names: 107021808

Gene position: 267719-266493 (Counterclockwise)

Preceding gene: 107021809

Following gene: 107021807

Centisome position: 8.13

GC content: 69.36

Gene sequence:

>1227_bases
ATGCAAACGAATCTCGCCGATTTCATCCGCAATACGCCCGACGGCGACGAGGCCGACGCGATCCTGCGCAAGTGCGTGCA
CTGCGGGTTCTGCACCGCGACCTGCCCGACCTATCAACTGCTCGGCGACGAACTCGACGGCCCGCGCGGCCGCATCTACC
TGATCAAGCAGATGGTCGAAGGCGCACCCGTCACGCGCAGCACGCAACAGCATCTCGACCGCTGCCTCACGTGCCGCAGT
TGCGAGACGACCTGCCCGTCCGGCGTCCAGTACGGCCGGCTCGTCGAGATCGGGCGCAAGCACGTCGAGGCGCAGGTGGA
ACGCCCCGCGCAGCAGCGGCTGATGCGCCGCGTGCTCGCGAGCGTCGTGCCGAACGCGGCGCTGTTCTCGCCGATGATGC
GGCTCGGCCAGCACGTGCGGCCGCTGCTGCCGAAGCGGCTGCGCGACAAGGTGCCGCCGCGCACGCGGCTGCTCGAATGG
CCGCACCGCACGCATCCGCGCAAGATGCTGATGCTCGGCGGCTGCGTGCAGCCGTCGATGCTGCCGAACATCAACATCGC
GACCGCGCGCGTGCTCGACGCGCTCGGCATCGAGACGGTCGTCGCGCCCGACGCGGGCTGCTGCGGCGCGATCCGCCTGC
ACCTGAACTATCACGACGAAGCGCTCGACGACGCGCGCCGCAACATCGACGCGTGGTGGCCCCACGTCGAACAGGGCGTC
GAGGCGATCGTGATGAACGCGTCGGGCTGCGGCGCGACGGTGCTCGAATACGCGCACCTGCTGCGCGACGATCCGGCCTA
CGCGGAGAAGGCGCAGCGCATCGTGTCGCTGACGCGCGACGTCTCCGACGTGCTGCTCGCGTTCGAGGCCGAACTCGCGA
CGATCGCGCGGCGCCGCGCGATCCATACGGTTGCCTATCACCCGCCGTGCACGCTGCAGCACGGCCAGCAGTCGCGCGGC
AAGGTCGAGCGCCTGCTGGAGACGCTCGGCATCGACGTGCGGCTGCCGGCCGACAGCCACCTGTGCTGCGGCTCGGCCGG
CACCTACTCGCTGACGCAGCCGTCGCTGTCGTACCGGTTGCGCAAGCAGAAGCTGCTGAAGCTGCAGGCGCTCGAGCCGC
AGATGATCGTGTCCGGCAACATCGGCTGCATCGCGCATCTGCAAAGCGGCACGCAGGTGCCGGTCGCCCACTGGGTCCAG
CTCGTCGAGCATTTGCTGTACGGCTAG

Upstream 100 bases:

>100_bases
CCCGCGCCGATGATGAAAATCCACCGCGGGCTGAAGAGCGCGTTCGATCCCGCGCGCATCTTCAATCGCGGCCGGCTTTA
CCCTGAGCTCTGAGGGCTGG

Downstream 100 bases:

>100_bases
GCCTCGTCCCGGCACGCAACGGGTGGACGGGCGCACGCGCGGCGTCCGTATAATGAGCGGATCGATGCGCCGCGTGTCGC
GGCGCATCGGCTTCATGCCC

Product: glycolate oxidase iron-sulfur subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 408; Mature: 408

Protein sequence:

>408_residues
MQTNLADFIRNTPDGDEADAILRKCVHCGFCTATCPTYQLLGDELDGPRGRIYLIKQMVEGAPVTRSTQQHLDRCLTCRS
CETTCPSGVQYGRLVEIGRKHVEAQVERPAQQRLMRRVLASVVPNAALFSPMMRLGQHVRPLLPKRLRDKVPPRTRLLEW
PHRTHPRKMLMLGGCVQPSMLPNINIATARVLDALGIETVVAPDAGCCGAIRLHLNYHDEALDDARRNIDAWWPHVEQGV
EAIVMNASGCGATVLEYAHLLRDDPAYAEKAQRIVSLTRDVSDVLLAFEAELATIARRRAIHTVAYHPPCTLQHGQQSRG
KVERLLETLGIDVRLPADSHLCCGSAGTYSLTQPSLSYRLRKQKLLKLQALEPQMIVSGNIGCIAHLQSGTQVPVAHWVQ
LVEHLLYG

Sequences:

>Translated_408_residues
MQTNLADFIRNTPDGDEADAILRKCVHCGFCTATCPTYQLLGDELDGPRGRIYLIKQMVEGAPVTRSTQQHLDRCLTCRS
CETTCPSGVQYGRLVEIGRKHVEAQVERPAQQRLMRRVLASVVPNAALFSPMMRLGQHVRPLLPKRLRDKVPPRTRLLEW
PHRTHPRKMLMLGGCVQPSMLPNINIATARVLDALGIETVVAPDAGCCGAIRLHLNYHDEALDDARRNIDAWWPHVEQGV
EAIVMNASGCGATVLEYAHLLRDDPAYAEKAQRIVSLTRDVSDVLLAFEAELATIARRRAIHTVAYHPPCTLQHGQQSRG
KVERLLETLGIDVRLPADSHLCCGSAGTYSLTQPSLSYRLRKQKLLKLQALEPQMIVSGNIGCIAHLQSGTQVPVAHWVQ
LVEHLLYG
>Mature_408_residues
MQTNLADFIRNTPDGDEADAILRKCVHCGFCTATCPTYQLLGDELDGPRGRIYLIKQMVEGAPVTRSTQQHLDRCLTCRS
CETTCPSGVQYGRLVEIGRKHVEAQVERPAQQRLMRRVLASVVPNAALFSPMMRLGQHVRPLLPKRLRDKVPPRTRLLEW
PHRTHPRKMLMLGGCVQPSMLPNINIATARVLDALGIETVVAPDAGCCGAIRLHLNYHDEALDDARRNIDAWWPHVEQGV
EAIVMNASGCGATVLEYAHLLRDDPAYAEKAQRIVSLTRDVSDVLLAFEAELATIARRRAIHTVAYHPPCTLQHGQQSRG
KVERLLETLGIDVRLPADSHLCCGSAGTYSLTQPSLSYRLRKQKLLKLQALEPQMIVSGNIGCIAHLQSGTQVPVAHWVQ
LVEHLLYG

Specific function: Unknown

COG id: COG0247

COG function: function code C; Fe-S oxidoreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI48994913, Length=407, Percent_Identity=53.0712530712531, Blast_Score=436, Evalue=1e-123,
Organism=Escherichia coli, GI1786497, Length=235, Percent_Identity=22.1276595744681, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1788576, Length=416, Percent_Identity=21.3942307692308, Blast_Score=72, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR004017
- InterPro:   IPR012285
- InterPro:   IPR012257 [H]

Pfam domain/function: PF02754 CCG [H]

EC number: NA

Molecular weight: Translated: 45340; Mature: 45340

Theoretical pI: Translated: 8.35; Mature: 8.35

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
3.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTNLADFIRNTPDGDEADAILRKCVHCGFCTATCPTYQLLGDELDGPRGRIYLIKQMVE
CCCCHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHC
GAPVTRSTQQHLDRCLTCRSCETTCPSGVQYGRLVEIGRKHVEAQVERPAQQRLMRRVLA
CCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
SVVPNAALFSPMMRLGQHVRPLLPKRLRDKVPPRTRLLEWPHRTHPRKMLMLGGCVQPSM
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCHHHHHCCCCCCCCC
LPNINIATARVLDALGIETVVAPDAGCCGAIRLHLNYHDEALDDARRNIDAWWPHVEQGV
CCCCCHHHHHHHHHHCCCEEECCCCCCCEEEEEEECCCHHHHHHHHHCCHHCCHHHHCCC
EAIVMNASGCGATVLEYAHLLRDDPAYAEKAQRIVSLTRDVSDVLLAFEAELATIARRRA
CEEEECCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IHTVAYHPPCTLQHGQQSRGKVERLLETLGIDVRLPADSHLCCGSAGTYSLTQPSLSYRL
HHHEEECCCCCCCCCCCHHHHHHHHHHHHCCCEECCCCCCEECCCCCCCCCCCCHHHHHH
RKQKLLKLQALEPQMIVSGNIGCIAHLQSGTQVPVAHWVQLVEHLLYG
HHHHHHHHHCCCCCEEEECCCCEEEEECCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MQTNLADFIRNTPDGDEADAILRKCVHCGFCTATCPTYQLLGDELDGPRGRIYLIKQMVE
CCCCHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHC
GAPVTRSTQQHLDRCLTCRSCETTCPSGVQYGRLVEIGRKHVEAQVERPAQQRLMRRVLA
CCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
SVVPNAALFSPMMRLGQHVRPLLPKRLRDKVPPRTRLLEWPHRTHPRKMLMLGGCVQPSM
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCHHHHHCCCCCCCCC
LPNINIATARVLDALGIETVVAPDAGCCGAIRLHLNYHDEALDDARRNIDAWWPHVEQGV
CCCCCHHHHHHHHHHCCCEEECCCCCCCEEEEEEECCCHHHHHHHHHCCHHCCHHHHCCC
EAIVMNASGCGATVLEYAHLLRDDPAYAEKAQRIVSLTRDVSDVLLAFEAELATIARRRA
CEEEECCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IHTVAYHPPCTLQHGQQSRGKVERLLETLGIDVRLPADSHLCCGSAGTYSLTQPSLSYRL
HHHEEECCCCCCCCCCCHHHHHHHHHHHHCCCEECCCCCCEECCCCCCCCCCCCHHHHHH
RKQKLLKLQALEPQMIVSGNIGCIAHLQSGTQVPVAHWVQLVEHLLYG
HHHHHHHHHCCCCCEEEECCCCEEEEECCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8606183; 9278503 [H]