| Definition | Burkholderia cenocepacia AU 1054 chromosome 1, complete sequence. |
|---|---|
| Accession | NC_008060 |
| Length | 3,294,563 |
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The map label for this gene is rlmL [H]
Identifier: 107021784
GI number: 107021784
Start: 237306
End: 238610
Strand: Reverse
Name: rlmL [H]
Synonym: Bcen_0224
Alternate gene names: 107021784
Gene position: 238610-237306 (Counterclockwise)
Preceding gene: 107021785
Following gene: 107021782
Centisome position: 7.24
GC content: 70.96
Gene sequence:
>1305_bases ATGTCCTCGCCCACCCTGTTCGAATTCTTCGCCCCCTGCCCGCGCGGCCTCGAAGCGGCGCTTGCCGCCGAGCTGGCCGA AATCGCCGGCCGCCACCTGAACGGCGCGCCGTTCACCGCGGGCGCGCAGGTGCCGGGCGGCGTCCATTTCAGCGGCGGCT GGGCCGCCGGCATGGCCGCGAACCTCCATTCGCGCATCGCGAGCCGGATTCTGCTGAAGATCGCGCACGGCCCGTACCGC AACGAACAGGACGTCTACGCGCTCGCGCTCGAGCAGCCGTGGGAACGCTGGTTCGCGTCGACGCAGACGCTGCGCATCGA CATCACCGCGATCAAGTCGCCGCTGAAGAGCCTCGAATTCACGACGCTGCGTGTGAAGGATGCGATCTGCGACCGGATGC GGGAGAAAACCGGCTCGCGCCCGAGCATCGACACCGGTGCGCCGGACGTGCGCGTGTTCGCGTTCCTGACCGTCAACGAG TGCACGCTGTACCTCGACACATCGGGCGAGCCGCTGTTCAAGCGCGGCTGGCGTCTCGACAAGGGGGCGGCGCCGCTGCG CGAGAACCTCGCGGCCGGCATCCTGCGCCTGACGGGCTGGACGCCCGGCACCGCGCTGTACGACCCGATGTGCGGCAGCG GCACGTTCCTCGCGGAAGCCGCGCAGATCGCGCTCGGCGTGGCGCCCGGCGTCGAGCGCCGGTTCGGCTTCGAAAAGCTC AAGCAGTACGACATCACCGCATGGCAGGGGCTGAAGGTCCCGGCGCTCGACGCGAAGCGGGCAGCGCGCGCCAAGCGCGG CGAAGCGCTCGGCGTGTACGGCAGCGACATCTCCGGCGACATGCTCGAGAAGGCGCGCGCGAACCTCGAACGCGCGGGCG TGCCGTCGGTGTGGCTCAAGCAGGTCGACGCGCGCGGGATGACGCCGCCGTGCGACGGGCCGGGCATCCTCCTCGCGAAC CCGCCGTACGGCGAGCGGATCGAAGTGCGCGGCCGCAGCGCGCGCGGCGAGGTGCGCGAAACCGGCCGCAACCGCGGCAA CGACGACGCATTCCGCCGCACGCACACCGACGCGCCGGACAGCGAGTTCTTCAACGCGCTCGGCGATGCGCTGAAGCAGC GCTTCACGGGCTGGCAGGCGTTCCTGCTGACGTCCGACCGTTCGCTGCCGGGCCAGTTGCGCCTGCGCGAATCGGCCAAG ACGCCGCTGTTCAACGGCGCGCTCGAATGCCGGCTGTTCCGCTTCGACCTGATCGCCGGCAGCGTGAAGGCGCGCCCGGC GGCGGTGGAAGGCGACGACGCGTAA
Upstream 100 bases:
>100_bases GCGCGAGCGGTCGTCGCCCCGACGCCGCCGCGCCGAAACGCCGGCCCCGGTCGCCCGCGCCGCGTACAATAGTGGACTTT TGCACGTCCTCACCCGCCTT
Downstream 100 bases:
>100_bases GCCAGCCGTTCGCTTCCACGCACAACGACTTCCAGGGGCCGCCATCGCGGCCCCTGTGCTTTTGCGCAGCCGGTCAGGGC TCCGACTGCGACCGGCGCGC
Product: RNA methylase
Products: NA
Alternate protein names: 23S rRNA m2G2445 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase rlmL [H]
Number of amino acids: Translated: 434; Mature: 433
Protein sequence:
>434_residues MSSPTLFEFFAPCPRGLEAALAAELAEIAGRHLNGAPFTAGAQVPGGVHFSGGWAAGMAANLHSRIASRILLKIAHGPYR NEQDVYALALEQPWERWFASTQTLRIDITAIKSPLKSLEFTTLRVKDAICDRMREKTGSRPSIDTGAPDVRVFAFLTVNE CTLYLDTSGEPLFKRGWRLDKGAAPLRENLAAGILRLTGWTPGTALYDPMCGSGTFLAEAAQIALGVAPGVERRFGFEKL KQYDITAWQGLKVPALDAKRAARAKRGEALGVYGSDISGDMLEKARANLERAGVPSVWLKQVDARGMTPPCDGPGILLAN PPYGERIEVRGRSARGEVRETGRNRGNDDAFRRTHTDAPDSEFFNALGDALKQRFTGWQAFLLTSDRSLPGQLRLRESAK TPLFNGALECRLFRFDLIAGSVKARPAAVEGDDA
Sequences:
>Translated_434_residues MSSPTLFEFFAPCPRGLEAALAAELAEIAGRHLNGAPFTAGAQVPGGVHFSGGWAAGMAANLHSRIASRILLKIAHGPYR NEQDVYALALEQPWERWFASTQTLRIDITAIKSPLKSLEFTTLRVKDAICDRMREKTGSRPSIDTGAPDVRVFAFLTVNE CTLYLDTSGEPLFKRGWRLDKGAAPLRENLAAGILRLTGWTPGTALYDPMCGSGTFLAEAAQIALGVAPGVERRFGFEKL KQYDITAWQGLKVPALDAKRAARAKRGEALGVYGSDISGDMLEKARANLERAGVPSVWLKQVDARGMTPPCDGPGILLAN PPYGERIEVRGRSARGEVRETGRNRGNDDAFRRTHTDAPDSEFFNALGDALKQRFTGWQAFLLTSDRSLPGQLRLRESAK TPLFNGALECRLFRFDLIAGSVKARPAAVEGDDA >Mature_433_residues SSPTLFEFFAPCPRGLEAALAAELAEIAGRHLNGAPFTAGAQVPGGVHFSGGWAAGMAANLHSRIASRILLKIAHGPYRN EQDVYALALEQPWERWFASTQTLRIDITAIKSPLKSLEFTTLRVKDAICDRMREKTGSRPSIDTGAPDVRVFAFLTVNEC TLYLDTSGEPLFKRGWRLDKGAAPLRENLAAGILRLTGWTPGTALYDPMCGSGTFLAEAAQIALGVAPGVERRFGFEKLK QYDITAWQGLKVPALDAKRAARAKRGEALGVYGSDISGDMLEKARANLERAGVPSVWLKQVDARGMTPPCDGPGILLANP PYGERIEVRGRSARGEVRETGRNRGNDDAFRRTHTDAPDSEFFNALGDALKQRFTGWQAFLLTSDRSLPGQLRLRESAKT PLFNGALECRLFRFDLIAGSVKARPAAVEGDDA
Specific function: Specifically methylates the guanosine in position 2445 (m2G2445) of 23S rRNA [H]
COG id: COG0116
COG function: function code L; Predicted N6-adenine-specific DNA methylase
Gene ontology:
Cell location: Cytoplasm (Potential) [H]
Metaboloic importance: Unknown [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 1 THUMP domain [H]
Homologues:
Organism=Escherichia coli, GI1787181, Length=393, Percent_Identity=33.0788804071247, Blast_Score=197, Evalue=1e-51,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR002052 - InterPro: IPR000241 - InterPro: IPR017244 - InterPro: IPR019614 - InterPro: IPR004114 [H]
Pfam domain/function: PF10672 Methyltrans_SAM; PF02926 THUMP; PF01170 UPF0020 [H]
EC number: =2.1.1.173 [H]
Molecular weight: Translated: 47199; Mature: 47068
Theoretical pI: Translated: 9.20; Mature: 9.20
Prosite motif: PS51165 THUMP ; PS00092 N6_MTASE
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.4 %Cys (Translated Protein) 1.4 %Met (Translated Protein) 2.8 %Cys+Met (Translated Protein) 1.4 %Cys (Mature Protein) 1.2 %Met (Mature Protein) 2.5 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MSSPTLFEFFAPCPRGLEAALAAELAEIAGRHLNGAPFTAGAQVPGGVHFSGGWAAGMAA CCCCCHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEECCCCHHHHHH NLHSRIASRILLKIAHGPYRNEQDVYALALEQPWERWFASTQTLRIDITAIKSPLKSLEF HHHHHHHHHHHHHHHCCCCCCCCCEEEEEHHHHHHHHHCCCCEEEEEHHHHHHHHHHCCE TTLRVKDAICDRMREKTGSRPSIDTGAPDVRVFAFLTVNECTLYLDTSGEPLFKRGWRLD EEEHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEECCEEEEEECCCCHHHHCCCCCC KGAAPLRENLAAGILRLTGWTPGTALYDPMCGSGTFLAEAAQIALGVAPGVERRFGFEKL CCCCHHHHHHHHHHHEEECCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHCHHHH KQYDITAWQGLKVPALDAKRAARAKRGEALGVYGSDISGDMLEKARANLERAGVPSVWLK HHHCCCCCCCCCCCCCCHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHCCCCHHHHH QVDARGMTPPCDGPGILLANPPYGERIEVRGRSARGEVRETGRNRGNDDAFRRTHTDAPD HHHCCCCCCCCCCCEEEEECCCCCCEEEECCCCCCCHHHHHCCCCCCCHHHHHCCCCCCH SEFFNALGDALKQRFTGWQAFLLTSDRSLPGQLRLRESAKTPLFNGALECRLFRFDLIAG HHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCEEEHHHCCCCCCCCCEEEEEEEEEEHHC SVKARPAAVEGDDA CCCCCCCCCCCCCC >Mature Secondary Structure SSPTLFEFFAPCPRGLEAALAAELAEIAGRHLNGAPFTAGAQVPGGVHFSGGWAAGMAA CCCCHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEECCCCHHHHHH NLHSRIASRILLKIAHGPYRNEQDVYALALEQPWERWFASTQTLRIDITAIKSPLKSLEF HHHHHHHHHHHHHHHCCCCCCCCCEEEEEHHHHHHHHHCCCCEEEEEHHHHHHHHHHCCE TTLRVKDAICDRMREKTGSRPSIDTGAPDVRVFAFLTVNECTLYLDTSGEPLFKRGWRLD EEEHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEECCEEEEEECCCCHHHHCCCCCC KGAAPLRENLAAGILRLTGWTPGTALYDPMCGSGTFLAEAAQIALGVAPGVERRFGFEKL CCCCHHHHHHHHHHHEEECCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHCHHHH KQYDITAWQGLKVPALDAKRAARAKRGEALGVYGSDISGDMLEKARANLERAGVPSVWLK HHHCCCCCCCCCCCCCCHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHCCCCHHHHH QVDARGMTPPCDGPGILLANPPYGERIEVRGRSARGEVRETGRNRGNDDAFRRTHTDAPD HHHCCCCCCCCCCCEEEEECCCCCCEEEECCCCCCCHHHHHCCCCCCCHHHHHCCCCCCH SEFFNALGDALKQRFTGWQAFLLTSDRSLPGQLRLRESAKTPLFNGALECRLFRFDLIAG HHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCEEEHHHCCCCCCCCCEEEEEEEEEEHHC SVKARPAAVEGDDA CCCCCCCCCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: NA