| Definition | Burkholderia cenocepacia AU 1054 chromosome 1, complete sequence. |
|---|---|
| Accession | NC_008060 |
| Length | 3,294,563 |
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The map label for this gene is purB [H]
Identifier: 107021743
GI number: 107021743
Start: 191676
End: 193064
Strand: Reverse
Name: purB [H]
Synonym: Bcen_0183
Alternate gene names: 107021743
Gene position: 193064-191676 (Counterclockwise)
Preceding gene: 107021750
Following gene: 107021741
Centisome position: 5.86
GC content: 65.73
Gene sequence:
>1389_bases ATGTCCGACACTCGTCCCGATACGCTTTTCGCCCTCACCGCGCTCTCGCCCATCGACGGCCGCTACGCCAGCAAGACCGA AGCGCTGCGCGACTGGCTCTCCGAAGCCGCCTTCATGCGCCACCGCGTCACCGTGGAGGTGCACTGGCTGATCGCGCTGT CGCGCGCCGGCTTCGCGGAAGTCCCGCGCTTCTCGGAAGCCTCCGAGCAGTTCCTGCTGCAGCTCGCCGAACGCTTCACC GCGCACGACGCCGCACGCATCAAGGAAATCGAGCGCGTGACGAACCACGACGTGAAGGCCGTCGAATACTGGCTGAAGGA ATCGGTGAAGGGCCAGGCCGAACTCGAGAAGGCGAGCGAATTCATCCACTTCGCGTGCACGTCCGAGGACATCAACAACA CGTCGCACGGGATGATGCTCGCCGGCGCGCGCGAACACGTGATCCTGCCGGCGCTGCGCACGGTCTACCAGCGCCTCGTC GCGCTCGCGCACGCGCACGCCGAGCAGCCGATGCTGTCGCGCACGCACGGCCAGCCGGCCAGCCCGACGACGCTCGGCAA GGAACTCGCGAACGTCGCCGCACGCCTTGCCCGCGCGATCACGCGCATCGAGAAGGTCGAGATCCTCGGCAAGATGAACG GCGCGGTCGGCAACTTCAACGCGCATCTCTCGGCGTATCCGGAATTCGACTGGGAAGCGTTCTCGCGCGACGTGATCGAG AACCGCCTGAAGCTCACGTTCAACCCGTACACGATCCAGATCGAGCCGCACGACTACATGGCCGAACTGTTCGACGCCGT GGCGCGCGCGAACACGATCCTGCTCGACCTCGACCGCGACGTGTGGGGCTACATCTCGGTCGGCTACTTCAAGCAGAAGA CGAAGGCCGGCGAAATCGGCTCGTCGACGATGCCGCACAAGGTCAACCCGATCGACTTCGAGAACTCGGAAGGCAACCTC GGCCTCGCGAACGCGACCCTGCGCCACCTCGCCGACAAACTGCCGGTGTCGCGCTGGCAGCGCGACCTGACCGACTCGAC GGTGCTGCGCAACATGGGCGTCGCGCTCGGCTACTCGCTGCTCGCGTACGACTCGCTGATCCGCGGCCTCGACAAGCTCG AAGTGAACCCGCAGCGCCTGAACGAAGATCTCGACAACTGCTGGGAAGTGCTCGCGGAGCCGGTGCAGACGGTGATGCGC CGCTACGGCATCGAGAACCCGTACGAGCAGCTGAAGGAACTGACGCGCGGCAAGGGCATCACGCGCGAAGCGCTGCAGGA ATTCGTCGGCACGCTGGCGATCCCGCAGGATGCGAAGAACCGCCTGCTCGCGATGACGCCGGCGTCGTACATCGGCAAGG CCGTCGAACTCGCGAAGCGGATCGCGTAA
Upstream 100 bases:
>100_bases CGCGGCCGGACCGGTTCGGCCGGAATCGACGCGCGCCGATGGTAAAATGCCGGATTCTCCTCCCCTCGTTCGCCCCGGCC CCAAAAGGCCCTGCCCGATC
Downstream 100 bases:
>100_bases GCACCGCCGTCGCCTGACGAAAAAAGCCCGATGCGAAGCGATTCGCATCGGGCTTTTTGTTGCCTGCCGCAGCCGATCAG AACGAGATCATCCGCCCCGG
Product: adenylosuccinate lyase
Products: NA
Alternate protein names: ASL; Adenylosuccinase; ASase [H]
Number of amino acids: Translated: 462; Mature: 461
Protein sequence:
>462_residues MSDTRPDTLFALTALSPIDGRYASKTEALRDWLSEAAFMRHRVTVEVHWLIALSRAGFAEVPRFSEASEQFLLQLAERFT AHDAARIKEIERVTNHDVKAVEYWLKESVKGQAELEKASEFIHFACTSEDINNTSHGMMLAGAREHVILPALRTVYQRLV ALAHAHAEQPMLSRTHGQPASPTTLGKELANVAARLARAITRIEKVEILGKMNGAVGNFNAHLSAYPEFDWEAFSRDVIE NRLKLTFNPYTIQIEPHDYMAELFDAVARANTILLDLDRDVWGYISVGYFKQKTKAGEIGSSTMPHKVNPIDFENSEGNL GLANATLRHLADKLPVSRWQRDLTDSTVLRNMGVALGYSLLAYDSLIRGLDKLEVNPQRLNEDLDNCWEVLAEPVQTVMR RYGIENPYEQLKELTRGKGITREALQEFVGTLAIPQDAKNRLLAMTPASYIGKAVELAKRIA
Sequences:
>Translated_462_residues MSDTRPDTLFALTALSPIDGRYASKTEALRDWLSEAAFMRHRVTVEVHWLIALSRAGFAEVPRFSEASEQFLLQLAERFT AHDAARIKEIERVTNHDVKAVEYWLKESVKGQAELEKASEFIHFACTSEDINNTSHGMMLAGAREHVILPALRTVYQRLV ALAHAHAEQPMLSRTHGQPASPTTLGKELANVAARLARAITRIEKVEILGKMNGAVGNFNAHLSAYPEFDWEAFSRDVIE NRLKLTFNPYTIQIEPHDYMAELFDAVARANTILLDLDRDVWGYISVGYFKQKTKAGEIGSSTMPHKVNPIDFENSEGNL GLANATLRHLADKLPVSRWQRDLTDSTVLRNMGVALGYSLLAYDSLIRGLDKLEVNPQRLNEDLDNCWEVLAEPVQTVMR RYGIENPYEQLKELTRGKGITREALQEFVGTLAIPQDAKNRLLAMTPASYIGKAVELAKRIA >Mature_461_residues SDTRPDTLFALTALSPIDGRYASKTEALRDWLSEAAFMRHRVTVEVHWLIALSRAGFAEVPRFSEASEQFLLQLAERFTA HDAARIKEIERVTNHDVKAVEYWLKESVKGQAELEKASEFIHFACTSEDINNTSHGMMLAGAREHVILPALRTVYQRLVA LAHAHAEQPMLSRTHGQPASPTTLGKELANVAARLARAITRIEKVEILGKMNGAVGNFNAHLSAYPEFDWEAFSRDVIEN RLKLTFNPYTIQIEPHDYMAELFDAVARANTILLDLDRDVWGYISVGYFKQKTKAGEIGSSTMPHKVNPIDFENSEGNLG LANATLRHLADKLPVSRWQRDLTDSTVLRNMGVALGYSLLAYDSLIRGLDKLEVNPQRLNEDLDNCWEVLAEPVQTVMRR YGIENPYEQLKELTRGKGITREALQEFVGTLAIPQDAKNRLLAMTPASYIGKAVELAKRIA
Specific function: De novo purine biosynthesis; eighth step. [C]
COG id: COG0015
COG function: function code F; Adenylosuccinate lyase
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]
Homologues:
Organism=Escherichia coli, GI1787376, Length=454, Percent_Identity=61.6740088105727, Blast_Score=583, Evalue=1e-167,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR013539 - InterPro: IPR000362 - InterPro: IPR020557 - InterPro: IPR008948 - InterPro: IPR022761 - InterPro: IPR004769 [H]
Pfam domain/function: PF08328 ASL_C; PF00206 Lyase_1 [H]
EC number: =4.3.2.2 [H]
Molecular weight: Translated: 51881; Mature: 51750
Theoretical pI: Translated: 6.52; Mature: 6.52
Prosite motif: PS00163 FUMARATE_LYASES
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.4 %Cys (Translated Protein) 2.4 %Met (Translated Protein) 2.8 %Cys+Met (Translated Protein) 0.4 %Cys (Mature Protein) 2.2 %Met (Mature Protein) 2.6 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MSDTRPDTLFALTALSPIDGRYASKTEALRDWLSEAAFMRHRVTVEVHWLIALSRAGFAE CCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEHHHCCCCHH VPRFSEASEQFLLQLAERFTAHDAARIKEIERVTNHDVKAVEYWLKESVKGQAELEKASE CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHH FIHFACTSEDINNTSHGMMLAGAREHVILPALRTVYQRLVALAHAHAEQPMLSRTHGQPA HHEEEECCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCC SPTTLGKELANVAARLARAITRIEKVEILGKMNGAVGNFNAHLSAYPEFDWEAFSRDVIE CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEECCCCCCHHHHHHHHHH NRLKLTFNPYTIQIEPHDYMAELFDAVARANTILLDLDRDVWGYISVGYFKQKTKAGEIG CCCEEEECCEEEEECCHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHHCCCCCCC SSTMPHKVNPIDFENSEGNLGLANATLRHLADKLPVSRWQRDLTDSTVLRNMGVALGYSL CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH LAYDSLIRGLDKLEVNPQRLNEDLDNCWEVLAEPVQTVMRRYGIENPYEQLKELTRGKGI HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCC TREALQEFVGTLAIPQDAKNRLLAMTPASYIGKAVELAKRIA CHHHHHHHHHHCCCCCHHHCCEEEECCHHHHHHHHHHHHHCC >Mature Secondary Structure SDTRPDTLFALTALSPIDGRYASKTEALRDWLSEAAFMRHRVTVEVHWLIALSRAGFAE CCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEHHHCCCCHH VPRFSEASEQFLLQLAERFTAHDAARIKEIERVTNHDVKAVEYWLKESVKGQAELEKASE CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHH FIHFACTSEDINNTSHGMMLAGAREHVILPALRTVYQRLVALAHAHAEQPMLSRTHGQPA HHEEEECCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCC SPTTLGKELANVAARLARAITRIEKVEILGKMNGAVGNFNAHLSAYPEFDWEAFSRDVIE CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEECCCCCCHHHHHHHHHH NRLKLTFNPYTIQIEPHDYMAELFDAVARANTILLDLDRDVWGYISVGYFKQKTKAGEIG CCCEEEECCEEEEECCHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHHCCCCCCC SSTMPHKVNPIDFENSEGNLGLANATLRHLADKLPVSRWQRDLTDSTVLRNMGVALGYSL CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH LAYDSLIRGLDKLEVNPQRLNEDLDNCWEVLAEPVQTVMRRYGIENPYEQLKELTRGKGI HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCC TREALQEFVGTLAIPQDAKNRLLAMTPASYIGKAVELAKRIA CHHHHHHHHHHCCCCCHHHCCEEEECCHHHHHHHHHHHHHCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 10984043 [H]