Click here to switch to the map view.

The map label for this gene is mnmC

Identifier: 107021566

GI number: 107021566

Start: 3294

End: 5225

Strand: Reverse

Name: mnmC

Synonym: Bcen_0004

Alternate gene names: 107021566

Gene position: 5225-3294 (Counterclockwise)

Preceding gene: 107021568

Following gene: 107021565

Centisome position: 0.16

GC content: 72.26

Gene sequence:

>1932_bases
ATGCCCGACCGACTTGTTTCCGCCACGCTCGCGCTTCGCGACGACGGCACGCTCGTCTCGCCCGAGTTCGGCGAGCTTCA
TCGCGGTGCATCCGGCACGCTCGCTCGCGCGCATCGCACGTTCGTCGCGGGCAACGGGCTGCCCGCGCGCTGGCAGCGCC
GCCGAACGTTCACGATCGTCACGACCGCGTTCGGCGCGGGCGCCGGCTTCCTCGCGGCCTGGGTCGCGTGGCGCGACGAT
CCCGCGCGCTGCGAACGGCTGCACGTCGTCGCGGTCGAACCGCATCCGTTCTCGCGCGACGACCTGCACCGCGCGGTCTC
GCATATGGTTGTAGACACAACCATATCGGCAGACGTCGACGCGTTGCTCGATGCCTGGCCGATACTCGTGCCGGGCCTGC
ACCGGCTCGAATTCGACGAAGGGCGCGTGGTGCTCACGCTCGCGTTCGGCGATACGATCGACTTGTTGAAGAAGCTCGTC
GCGCGCGCCGATGCGTTTTTTCTCGACGGCGCCGCGGCATCGGGCGACGGGATTCGCGCACTCGCGAAACTCGCCGGCGA
ACACGCGACGTTTGCGACGCATGCAAAGTCCGACGACGTGAAACACGCGCTCGGCGAAACAGGTTTCACGTTCCGCGAAG
TCGACGATCGCCTCGTCGGCGACTATGCGCCGCGCTGGCGCGCGCGCCGGCACGAGCCGCCGCGTGCGCTGCCCGTTGCC
GCGCGCCGCGCGATCGTGATCGGCGCGGGTCTCGCCGGCTGCGCGGTGGTCGAACGGCTGGCCGCGCGCGGCTGGGACGT
CACGCTGATCGAACGGCACGAACGGATCGCGAGCGAAGCGTCGGGCAATCCGGCCGGTGTGTTCCATCCATTGATGACGC
GCGATGACAACGTCGCGAGCCGGCTCACGCGCGGCGGCTTCCTGCACGCGCTCGCACGCTGGCGTGCGCTCGAACGCGCG
GGGCATGCATTCTCACGCAGCACGCACGGGATGCTCCATCTCGCCGAATCGGCCGACGATTTCGCGCGGATGCGCGACGC
GTTCGACGCGTTCGGGCCGCCGTCCGACTATGCAACGCTGCTCGATGCCGATGCCGCGCGGGCGCACCTGAATCTGCCGG
TCGCGCAGGGCGGCCTGCTGTTTCCGCACGGCGGCGCCGTGTGGCCGGCCGGGCTGTGCGTCGCGCAGTATGCGGCGGCC
GGCGAGCGCGTGCGGCTGCTGGCGTCCACCTGCGTCGCACGGCTCGAACGGCGGGACGACACCTGGCATGCACTCGACGA
TACCGGCGCCACGCTGGCCGATGCGCCCGTCGTCGTGCTCGCGAACGCGGGCGACGCCGCGCGTCTTGCCGGGCTGCGGC
ACGTCACGCTGCAACCGGTGCGCGGCCAGCTCACGCTGTTGCCGCCGGGCACGACGGCGCCGCTGCCGTGCCCGGCGATC
GGCGACGGTTACGCGGTCCCGCTCGACGACGGCACGCTGCTGATCGGCGCCACCTTCGAACCGGACGACACCGATCCCGC
GATGCGCGCGGCCGGCCACGCCGAAAACCTCGATCGCGTGCGGCACCTGCTGCCGGGCCTGATCGGCGCGCTGCCGGATC
CCGCCACGCTGCGCGGGCGCGTCGCATTCCGCTGGGTCGTCGGCGACCGCCTGCCGCTCATCGGCCCGCTCGCCGACGAA
ACGCAGGCCACCGCGAACGCACGCGCGCTGGGCGGTGCGCAGGCGCGCGACCTGCCGCGCATGCCGGGCCTCTACGGCGC
CTTCGGCTTCGGTTCGCGCGGCCTCGTGTGGGCCGCGCTCGGCGCCGAACTCATCGCATCGCAACTCGAAGGCGAGCCCT
GGCCGCTCGAGCGCGAACTCGCCGACGCCGTCGATCCCGCCCGCTTCCTGATCCGCGCACTGCGCGCCCGCCGCGTCGGC
TCGGCGGGCTGA

Upstream 100 bases:

>100_bases
TGGTCCGCCCGATCGTCGCGCCGTTGCCCCTTTCCAACGGCCGGTTGGACGGCCTCCCGCAATCCCTTGCCAGGCTTGGC
TGCCACGTTTTTTGTTTCGC

Downstream 100 bases:

>100_bases
CCTCGCGCGCACCCCCTCACAGTTTCCCGATTTTTCCGCGCAAGGTTATCCACGCGATGCTGTGGATAACCGCGCGGAAT
CCGCGCGCACTTCGTGTGCA

Product: 5-methylaminomethyl-2-thiouridine methyltransferase

Products: NA

Alternate protein names: tRNA mnm(5)s(2)U biosynthesis bifunctional protein; tRNA (mnm(5)s(2)U34)-methyltransferase; FAD-dependent cmnm(5)s(2)U34 oxidoreductase

Number of amino acids: Translated: 643; Mature: 642

Protein sequence:

>643_residues
MPDRLVSATLALRDDGTLVSPEFGELHRGASGTLARAHRTFVAGNGLPARWQRRRTFTIVTTAFGAGAGFLAAWVAWRDD
PARCERLHVVAVEPHPFSRDDLHRAVSHMVVDTTISADVDALLDAWPILVPGLHRLEFDEGRVVLTLAFGDTIDLLKKLV
ARADAFFLDGAAASGDGIRALAKLAGEHATFATHAKSDDVKHALGETGFTFREVDDRLVGDYAPRWRARRHEPPRALPVA
ARRAIVIGAGLAGCAVVERLAARGWDVTLIERHERIASEASGNPAGVFHPLMTRDDNVASRLTRGGFLHALARWRALERA
GHAFSRSTHGMLHLAESADDFARMRDAFDAFGPPSDYATLLDADAARAHLNLPVAQGGLLFPHGGAVWPAGLCVAQYAAA
GERVRLLASTCVARLERRDDTWHALDDTGATLADAPVVVLANAGDAARLAGLRHVTLQPVRGQLTLLPPGTTAPLPCPAI
GDGYAVPLDDGTLLIGATFEPDDTDPAMRAAGHAENLDRVRHLLPGLIGALPDPATLRGRVAFRWVVGDRLPLIGPLADE
TQATANARALGGAQARDLPRMPGLYGAFGFGSRGLVWAALGAELIASQLEGEPWPLERELADAVDPARFLIRALRARRVG
SAG

Sequences:

>Translated_643_residues
MPDRLVSATLALRDDGTLVSPEFGELHRGASGTLARAHRTFVAGNGLPARWQRRRTFTIVTTAFGAGAGFLAAWVAWRDD
PARCERLHVVAVEPHPFSRDDLHRAVSHMVVDTTISADVDALLDAWPILVPGLHRLEFDEGRVVLTLAFGDTIDLLKKLV
ARADAFFLDGAAASGDGIRALAKLAGEHATFATHAKSDDVKHALGETGFTFREVDDRLVGDYAPRWRARRHEPPRALPVA
ARRAIVIGAGLAGCAVVERLAARGWDVTLIERHERIASEASGNPAGVFHPLMTRDDNVASRLTRGGFLHALARWRALERA
GHAFSRSTHGMLHLAESADDFARMRDAFDAFGPPSDYATLLDADAARAHLNLPVAQGGLLFPHGGAVWPAGLCVAQYAAA
GERVRLLASTCVARLERRDDTWHALDDTGATLADAPVVVLANAGDAARLAGLRHVTLQPVRGQLTLLPPGTTAPLPCPAI
GDGYAVPLDDGTLLIGATFEPDDTDPAMRAAGHAENLDRVRHLLPGLIGALPDPATLRGRVAFRWVVGDRLPLIGPLADE
TQATANARALGGAQARDLPRMPGLYGAFGFGSRGLVWAALGAELIASQLEGEPWPLERELADAVDPARFLIRALRARRVG
SAG
>Mature_642_residues
PDRLVSATLALRDDGTLVSPEFGELHRGASGTLARAHRTFVAGNGLPARWQRRRTFTIVTTAFGAGAGFLAAWVAWRDDP
ARCERLHVVAVEPHPFSRDDLHRAVSHMVVDTTISADVDALLDAWPILVPGLHRLEFDEGRVVLTLAFGDTIDLLKKLVA
RADAFFLDGAAASGDGIRALAKLAGEHATFATHAKSDDVKHALGETGFTFREVDDRLVGDYAPRWRARRHEPPRALPVAA
RRAIVIGAGLAGCAVVERLAARGWDVTLIERHERIASEASGNPAGVFHPLMTRDDNVASRLTRGGFLHALARWRALERAG
HAFSRSTHGMLHLAESADDFARMRDAFDAFGPPSDYATLLDADAARAHLNLPVAQGGLLFPHGGAVWPAGLCVAQYAAAG
ERVRLLASTCVARLERRDDTWHALDDTGATLADAPVVVLANAGDAARLAGLRHVTLQPVRGQLTLLPPGTTAPLPCPAIG
DGYAVPLDDGTLLIGATFEPDDTDPAMRAAGHAENLDRVRHLLPGLIGALPDPATLRGRVAFRWVVGDRLPLIGPLADET
QATANARALGGAQARDLPRMPGLYGAFGFGSRGLVWAALGAELIASQLEGEPWPLERELADAVDPARFLIRALRARRVGS
AG

Specific function: Catalyzes the last two steps in the biosynthesis of 5- methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at the wobble position (U34) in tRNA. Catalyzes the FAD-dependent demodification of cmnm(5)s(2)U34 to nm(5)s(2)U34, followed by the transfer of a methyl g

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the DAO family

Homologues:

Organism=Escherichia coli, GI87082084, Length=680, Percent_Identity=27.7941176470588, Blast_Score=200, Evalue=2e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNMC_BURCA (Q1BZN5)

Other databases:

- EMBL:   CP000378
- RefSeq:   YP_619893.1
- ProteinModelPortal:   Q1BZN5
- SMR:   Q1BZN5
- STRING:   Q1BZN5
- GeneID:   4091874
- GenomeReviews:   CP000378_GR
- KEGG:   bcn:Bcen_0004
- eggNOG:   COG0665
- HOGENOM:   HBG677640
- OMA:   VIGETGF
- ProtClustDB:   PRK01747
- BioCyc:   BCEN331271:BCEN_0004-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01102
- InterPro:   IPR008471
- InterPro:   IPR006076
- InterPro:   IPR023032
- InterPro:   IPR017610
- TIGRFAMs:   TIGR03197

Pfam domain/function: PF01266 DAO; PF05430 DUF752

EC number: =2.1.1.61

Molecular weight: Translated: 68672; Mature: 68541

Theoretical pI: Translated: 6.94; Mature: 6.94

Prosite motif: PS00646 RIBOSOMAL_S13_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDRLVSATLALRDDGTLVSPEFGELHRGASGTLARAHRTFVAGNGLPARWQRRRTFTIV
CCCCCEEEEEEEECCCCEECCCHHHHHCCCCCCHHHHHHHEEECCCCCHHHHCCEEEEEE
TTAFGAGAGFLAAWVAWRDDPARCERLHVVAVEPHPFSRDDLHRAVSHMVVDTTISADVD
EECCCCCHHHHHHHHHCCCCHHHCCEEEEEEECCCCCCHHHHHHHHHHHHHHHEHHCCHH
ALLDAWPILVPGLHRLEFDEGRVVLTLAFGDTIDLLKKLVARADAFFLDGAAASGDGIRA
HHHHHHHHHCCCCCEEEECCCCEEEEEECCCHHHHHHHHHHHHCCEEECCCCCCCHHHHH
LAKLAGEHATFATHAKSDDVKHALGETGFTFREVDDRLVGDYAPRWRARRHEPPRALPVA
HHHHHCCCCEEEECCCCCHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHCCCCCCCCCHH
ARRAIVIGAGLAGCAVVERLAARGWDVTLIERHERIASEASGNPAGVFHPLMTRDDNVAS
HCCEEEEECCHHHHHHHHHHHHCCCCEEEHHHHHHHHHHCCCCCCEEECHHHCCCCHHHH
RLTRGGFLHALARWRALERAGHAFSRSTHGMLHLAESADDFARMRDAFDAFGPPSDYATL
HHHCCHHHHHHHHHHHHHHHCHHHHCCCCCEEEECCCHHHHHHHHHHHHCCCCCCCHHHH
LDADAARAHLNLPVAQGGLLFPHGGAVWPAGLCVAQYAAAGERVRLLASTCVARLERRDD
HCCCHHHHEECCEEECCCEEECCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCC
TWHALDDTGATLADAPVVVLANAGDAARLAGLRHVTLQPVRGQLTLLPPGTTAPLPCPAI
CCEECCCCCCEECCCCEEEEECCCCHHHHHCCCEEEEECCCCEEEEECCCCCCCCCCCCC
GDGYAVPLDDGTLLIGATFEPDDTDPAMRAAGHAENLDRVRHLLPGLIGALPDPATLRGR
CCCEEEECCCCEEEEEEECCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCE
VAFRWVVGDRLPLIGPLADETQATANARALGGAQARDLPRMPGLYGAFGFGSRGLVWAAL
EEEEEEECCCCCCCCCCCCCHHHHCCHHHCCCCCCCCCCCCCCCCEECCCCCCCHHHHHH
GAELIASQLEGEPWPLERELADAVDPARFLIRALRARRVGSAG
HHHHHHHHHCCCCCCCHHHHHHHCCHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
PDRLVSATLALRDDGTLVSPEFGELHRGASGTLARAHRTFVAGNGLPARWQRRRTFTIV
CCCCEEEEEEEECCCCEECCCHHHHHCCCCCCHHHHHHHEEECCCCCHHHHCCEEEEEE
TTAFGAGAGFLAAWVAWRDDPARCERLHVVAVEPHPFSRDDLHRAVSHMVVDTTISADVD
EECCCCCHHHHHHHHHCCCCHHHCCEEEEEEECCCCCCHHHHHHHHHHHHHHHEHHCCHH
ALLDAWPILVPGLHRLEFDEGRVVLTLAFGDTIDLLKKLVARADAFFLDGAAASGDGIRA
HHHHHHHHHCCCCCEEEECCCCEEEEEECCCHHHHHHHHHHHHCCEEECCCCCCCHHHHH
LAKLAGEHATFATHAKSDDVKHALGETGFTFREVDDRLVGDYAPRWRARRHEPPRALPVA
HHHHHCCCCEEEECCCCCHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHCCCCCCCCCHH
ARRAIVIGAGLAGCAVVERLAARGWDVTLIERHERIASEASGNPAGVFHPLMTRDDNVAS
HCCEEEEECCHHHHHHHHHHHHCCCCEEEHHHHHHHHHHCCCCCCEEECHHHCCCCHHHH
RLTRGGFLHALARWRALERAGHAFSRSTHGMLHLAESADDFARMRDAFDAFGPPSDYATL
HHHCCHHHHHHHHHHHHHHHCHHHHCCCCCEEEECCCHHHHHHHHHHHHCCCCCCCHHHH
LDADAARAHLNLPVAQGGLLFPHGGAVWPAGLCVAQYAAAGERVRLLASTCVARLERRDD
HCCCHHHHEECCEEECCCEEECCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCC
TWHALDDTGATLADAPVVVLANAGDAARLAGLRHVTLQPVRGQLTLLPPGTTAPLPCPAI
CCEECCCCCCEECCCCEEEEECCCCHHHHHCCCEEEEECCCCEEEEECCCCCCCCCCCCC
GDGYAVPLDDGTLLIGATFEPDDTDPAMRAAGHAENLDRVRHLLPGLIGALPDPATLRGR
CCCEEEECCCCEEEEEEECCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCE
VAFRWVVGDRLPLIGPLADETQATANARALGGAQARDLPRMPGLYGAFGFGSRGLVWAAL
EEEEEEECCCCCCCCCCCCCHHHHCCHHHCCCCCCCCCCCCCCCCEECCCCCCCHHHHHH
GAELIASQLEGEPWPLERELADAVDPARFLIRALRARRVGSAG
HHHHHHHHHCCCCCCCHHHHHHHCCHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA