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The map label for this gene is pntB [H]

Identifier: 104779421

GI number: 104779421

Start: 107844

End: 109280

Strand: Reverse

Name: pntB [H]

Synonym: PSEEN0114

Alternate gene names: 104779421

Gene position: 109280-107844 (Counterclockwise)

Preceding gene: 104779422

Following gene: 104779420

Centisome position: 1.86

GC content: 63.74

Gene sequence:

>1437_bases
ATGAGCATGAATCTGGTAACGCTTCTGTACCTGGTCGCCTCGATCTGCTTCATCCAGGCGCTCAAGGGCCTCTCGCACCC
GACCACCTCACGCCGCGGCAACCTGTTCGGCATGATCGGCATGGGGATCGCGATCCTCACCACGGTCGGGCTCATCTACA
AGCTGGGCGCCGAACTGGCTACCGCCGGCATCGGCTATGTGATCGTCGGCCTGCTGATCGGCGGCACCGCCGGCTCGATC
ATGGCCAAGCGCGTCGAGATGACCAAGATGCCCGAGCTGGTCGCCTTCATGCACAGCATGATCGGCCTGGCCGCGGTGTT
CATCGCCATCGCCGCCGTGCTCGAACCGCAATCGCTGGGCATCGTCGCCAGCATCAGCGACCCGATCCCCACCGGCAACC
GCCTGGAACTGTTCCTGGGCGCGGCCATCGGTGCTATCACCTTCTCGGGTTCTGTGATCGCCTTCGGCAAGCTGTCGGGC
AAGTACAAGTTCCGCCTGTTCCAAGGCGCACCGGTACAGTTCGCCGGCCAGCACAAGCTGAACCTGATCCTGGGCCTGGC
GACCATCGCCCTTGGGCTGCTGTTCACCTTCACCGGCCACTACAGCGCCTTCACCCTGATGCTGGCCCTGGCCTTCGTCA
TGGGCGTGCTGATCATCATCCCGATCGGCGGCGCCGACATGCCGGTGGTGGTGTCGATGCTCAACAGCTATTCGGGCTGG
GCGGCGGCCGGGATCGGCTTCTCGCTGAACAACTCGATGCTGATCATCGCAGGTTCCCTGGTGGGCTCCAGCGGTGCGAT
CCTCTCGTACATCATGTGCAAGGCGATGAACCGCTCGTTCTTCAACGTCATCCTCGGTGGCTTCGGCGGCGATACCGATA
CCGGCGCGACGCAGGGCTCGAAGGAGCAGCGCCCGGTGAAGTCCGGCTCGGCCGACGACGCCACCTTCCTGCTGAGCAAC
GCCGACAGCGTGATCATTGTCCCCGGCTATGGCCTGGCGGTGGCCCGCGCCCAGCACGCGCTCAAGGAACTGACCGAGAA
GCTGACCCACAACGGTGTGACCGTGAAGTACGCGATCCACCCGGTGGCAGGGCGCATGCCCGGGCACATGAACGTGCTGC
TGGCCGAGGCCGAGGTGCCGTACGACCAGGTGTTCGAGATGGAAGACATCAACGCCGAGTTCGGCCAGGCCGACGTGGTA
CTGGTGCTGGGCGCCAACGACGTGGTCAACCCGGCGGCGAAGAACGATCCGAAGTCGCCGATCGCCGGCATGCCGATCCT
CGAAGCCTTCAAGGCCAAGACCATCATCGTCAACAAGCGCTCCATGGCCAGCGGCTACGCCGGCCTGGACAACGAACTGT
TCTACCTGGACAAGACCATGATGGTGTTCGGTGACGCGAAGAAGGTCATCGAGGACATGGTCAAGGCAGTGGACTGA

Upstream 100 bases:

>100_bases
TGGCGCTGGCCGCGGTCAACGTGTTCGGTGGCTTCCTGGTCACCCGCCGCATGCTGGAAATGTTCAAGAAGAAAACCAAG
AACGAGGCGCAGAAGTAAGC

Downstream 100 bases:

>100_bases
CCAAGCCTTCTATATATAGAAGCGCATAAGGCCCCCGGGCGATATTCCCGGGGGCCTTTTCGTTTGCTTGATCCAGATCA
ACGGCTCTATTTCCGGCCTT

Product: pyridine nucleotide transhydrogenase subunit beta

Products: NA

Alternate protein names: Nicotinamide nucleotide transhydrogenase subunit beta; Proton-translocating transhydrogenase NADP(H)-binding component; Pyridine nucleotide transhydrogenase subunit beta; dIII [H]

Number of amino acids: Translated: 478; Mature: 477

Protein sequence:

>478_residues
MSMNLVTLLYLVASICFIQALKGLSHPTTSRRGNLFGMIGMGIAILTTVGLIYKLGAELATAGIGYVIVGLLIGGTAGSI
MAKRVEMTKMPELVAFMHSMIGLAAVFIAIAAVLEPQSLGIVASISDPIPTGNRLELFLGAAIGAITFSGSVIAFGKLSG
KYKFRLFQGAPVQFAGQHKLNLILGLATIALGLLFTFTGHYSAFTLMLALAFVMGVLIIIPIGGADMPVVVSMLNSYSGW
AAAGIGFSLNNSMLIIAGSLVGSSGAILSYIMCKAMNRSFFNVILGGFGGDTDTGATQGSKEQRPVKSGSADDATFLLSN
ADSVIIVPGYGLAVARAQHALKELTEKLTHNGVTVKYAIHPVAGRMPGHMNVLLAEAEVPYDQVFEMEDINAEFGQADVV
LVLGANDVVNPAAKNDPKSPIAGMPILEAFKAKTIIVNKRSMASGYAGLDNELFYLDKTMMVFGDAKKVIEDMVKAVD

Sequences:

>Translated_478_residues
MSMNLVTLLYLVASICFIQALKGLSHPTTSRRGNLFGMIGMGIAILTTVGLIYKLGAELATAGIGYVIVGLLIGGTAGSI
MAKRVEMTKMPELVAFMHSMIGLAAVFIAIAAVLEPQSLGIVASISDPIPTGNRLELFLGAAIGAITFSGSVIAFGKLSG
KYKFRLFQGAPVQFAGQHKLNLILGLATIALGLLFTFTGHYSAFTLMLALAFVMGVLIIIPIGGADMPVVVSMLNSYSGW
AAAGIGFSLNNSMLIIAGSLVGSSGAILSYIMCKAMNRSFFNVILGGFGGDTDTGATQGSKEQRPVKSGSADDATFLLSN
ADSVIIVPGYGLAVARAQHALKELTEKLTHNGVTVKYAIHPVAGRMPGHMNVLLAEAEVPYDQVFEMEDINAEFGQADVV
LVLGANDVVNPAAKNDPKSPIAGMPILEAFKAKTIIVNKRSMASGYAGLDNELFYLDKTMMVFGDAKKVIEDMVKAVD
>Mature_477_residues
SMNLVTLLYLVASICFIQALKGLSHPTTSRRGNLFGMIGMGIAILTTVGLIYKLGAELATAGIGYVIVGLLIGGTAGSIM
AKRVEMTKMPELVAFMHSMIGLAAVFIAIAAVLEPQSLGIVASISDPIPTGNRLELFLGAAIGAITFSGSVIAFGKLSGK
YKFRLFQGAPVQFAGQHKLNLILGLATIALGLLFTFTGHYSAFTLMLALAFVMGVLIIIPIGGADMPVVVSMLNSYSGWA
AAGIGFSLNNSMLIIAGSLVGSSGAILSYIMCKAMNRSFFNVILGGFGGDTDTGATQGSKEQRPVKSGSADDATFLLSNA
DSVIIVPGYGLAVARAQHALKELTEKLTHNGVTVKYAIHPVAGRMPGHMNVLLAEAEVPYDQVFEMEDINAEFGQADVVL
VLGANDVVNPAAKNDPKSPIAGMPILEAFKAKTIIVNKRSMASGYAGLDNELFYLDKTMMVFGDAKKVIEDMVKAVD

Specific function: The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane [H]

COG id: COG1282

COG function: function code C; NAD/NADP transhydrogenase beta subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNT beta subunit family [H]

Homologues:

Organism=Homo sapiens, GI122939155, Length=476, Percent_Identity=46.6386554621849, Blast_Score=387, Evalue=1e-107,
Organism=Homo sapiens, GI122939153, Length=476, Percent_Identity=46.6386554621849, Blast_Score=387, Evalue=1e-107,
Organism=Escherichia coli, GI1787886, Length=482, Percent_Identity=52.0746887966805, Blast_Score=467, Evalue=1e-133,
Organism=Caenorhabditis elegans, GI17550456, Length=478, Percent_Identity=45.6066945606695, Blast_Score=359, Evalue=2e-99,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012136
- InterPro:   IPR004003 [H]

Pfam domain/function: PF02233 PNTB [H]

EC number: =1.6.1.2 [H]

Molecular weight: Translated: 50156; Mature: 50025

Theoretical pI: Translated: 7.71; Mature: 7.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMNLVTLLYLVASICFIQALKGLSHPTTSRRGNLFGMIGMGIAILTTVGLIYKLGAELA
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHH
TAGIGYVIVGLLIGGTAGSIMAKRVEMTKMPELVAFMHSMIGLAAVFIAIAAVLEPQSLG
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
IVASISDPIPTGNRLELFLGAAIGAITFSGSVIAFGKLSGKYKFRLFQGAPVQFAGQHKL
EEEECCCCCCCCCCEEEEEHHHHHHEEECCCEEEEEECCCEEEEEEEECCCCEECCCCHH
NLILGLATIALGLLFTFTGHYSAFTLMLALAFVMGVLIIIPIGGADMPVVVSMLNSYSGW
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHCCCCC
AAAGIGFSLNNSMLIIAGSLVGSSGAILSYIMCKAMNRSFFNVILGGFGGDTDTGATQGS
EEECCCEEECCCEEEEEECCCCCCCHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCCCC
KEQRPVKSGSADDATFLLSNADSVIIVPGYGLAVARAQHALKELTEKLTHNGVTVKYAIH
CCCCCCCCCCCCCCEEEEECCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEE
PVAGRMPGHMNVLLAEAEVPYDQVFEMEDINAEFGQADVVLVLGANDVVNPAAKNDPKSP
ECCCCCCCCCEEEEEECCCCHHHHCCHHCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCC
IAGMPILEAFKAKTIIVNKRSMASGYAGLDNELFYLDKTMMVFGDAKKVIEDMVKAVD
CCCCHHHHHHCCCEEEEECCHHHCCCCCCCCCEEEEEEEHEEECCHHHHHHHHHHHCC
>Mature Secondary Structure 
SMNLVTLLYLVASICFIQALKGLSHPTTSRRGNLFGMIGMGIAILTTVGLIYKLGAELA
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHH
TAGIGYVIVGLLIGGTAGSIMAKRVEMTKMPELVAFMHSMIGLAAVFIAIAAVLEPQSLG
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
IVASISDPIPTGNRLELFLGAAIGAITFSGSVIAFGKLSGKYKFRLFQGAPVQFAGQHKL
EEEECCCCCCCCCCEEEEEHHHHHHEEECCCEEEEEECCCEEEEEEEECCCCEECCCCHH
NLILGLATIALGLLFTFTGHYSAFTLMLALAFVMGVLIIIPIGGADMPVVVSMLNSYSGW
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHCCCCC
AAAGIGFSLNNSMLIIAGSLVGSSGAILSYIMCKAMNRSFFNVILGGFGGDTDTGATQGS
EEECCCEEECCCEEEEEECCCCCCCHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCCCC
KEQRPVKSGSADDATFLLSNADSVIIVPGYGLAVARAQHALKELTEKLTHNGVTVKYAIH
CCCCCCCCCCCCCCEEEEECCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEE
PVAGRMPGHMNVLLAEAEVPYDQVFEMEDINAEFGQADVVLVLGANDVVNPAAKNDPKSP
ECCCCCCCCCEEEEEECCCCHHHHCCHHCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCC
IAGMPILEAFKAKTIIVNKRSMASGYAGLDNELFYLDKTMMVFGDAKKVIEDMVKAVD
CCCCHHHHHHCCCEEEEECCHHHCCCCCCCCCEEEEEEEHEEECCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8075801 [H]