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The map label for this gene is trpA

Identifier: 104779350

GI number: 104779350

Start: 38408

End: 39217

Strand: Reverse

Name: trpA

Synonym: PSEEN0037

Alternate gene names: 104779350

Gene position: 39217-38408 (Counterclockwise)

Preceding gene: 104779351

Following gene: 104779347

Centisome position: 0.67

GC content: 67.53

Gene sequence:

>810_bases
ATGAGCCGTCTCGAACAACGCTTCGCCGAATTGAAGGCCGAAGGCCGCTCCGCCCTGGTCACCTTCGTCACCGCCGGCGA
CCCGGGCTACGACGCCTCGCTGGAGATCCTCAAGGGCCTGCCGGCCGCTGGCGCCGATGTGATCGAACTGGGCATGCCGT
TCACCGACCCGATGGCCGACGGCGTCGCTATCCAGCTCGCCACCCTGCGTGCCCTGGAAGCCGGTCAGACACTGGCCAAG
ACCCTGCAGATGGTTCGCGAATTCCGTGTCGGCAACCAGACCACGCCCATCGTGCTGATGGGTTACTACAACCCGATCCA
CCGTTTCGGCGTTGAGCAGTTCGTCGCCGAGGCGAAAGAGGCGGGCGTAGACGGCCTGATCATCGTCGACCTGCCTCCGG
AGCACGACGCCGAACTGGCCACCCCAGCCCAGGCCGCAGGCATCGACTTCATCCGCCTGACCACCCCGACTACCGACGAT
GCGCGCCTGCCGCGCGTGCTGGAGCGCAGCTCCGGGTTCGTCTACTACGTGTCGGTGGCCGGGGTGACCGGTGCGGGCTC
GGCGACCACCGAACATGTGACCGAAGCCATTGCGCGCCTGCGCCGGCATACCGACCTGCCGATCAGCGTCGGTTTCGGCA
TCCGCACACCGGAGCAGGCTGCTGCCATTGCCCGCCTGGCTGATGGCGTGGTGGTCGGTTCGGCGCTGGTCGACAAGATC
GCCCAGGCCAAGGATGCCGGCCAGGCGGTGAAGGATGTGTTGAGCCTGTGCTCGGCGCTGGCCGACGGCGTACGCGCCGC
TCGCGTCTGA

Upstream 100 bases:

>100_bases
AGCTGCCCAAGGACCACCTGATGGTGATCTGCCTGTCCGGTCGCGGCGACAAGGACATGCAAACCGTGATGAACCACATG
GCCGCCCAGGAGAAACAGGC

Downstream 100 bases:

>100_bases
TTCGCGTCGTCTTGTTCGCCGGCAAGGCCGGCTCCTACAGGTATACGACGATCATCTGTAGGAGCCGGCCTTGCCGGCGA
ACAGCGTCAGCCGGCGCAAC

Product: tryptophan synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 269; Mature: 268

Protein sequence:

>269_residues
MSRLEQRFAELKAEGRSALVTFVTAGDPGYDASLEILKGLPAAGADVIELGMPFTDPMADGVAIQLATLRALEAGQTLAK
TLQMVREFRVGNQTTPIVLMGYYNPIHRFGVEQFVAEAKEAGVDGLIIVDLPPEHDAELATPAQAAGIDFIRLTTPTTDD
ARLPRVLERSSGFVYYVSVAGVTGAGSATTEHVTEAIARLRRHTDLPISVGFGIRTPEQAAAIARLADGVVVGSALVDKI
AQAKDAGQAVKDVLSLCSALADGVRAARV

Sequences:

>Translated_269_residues
MSRLEQRFAELKAEGRSALVTFVTAGDPGYDASLEILKGLPAAGADVIELGMPFTDPMADGVAIQLATLRALEAGQTLAK
TLQMVREFRVGNQTTPIVLMGYYNPIHRFGVEQFVAEAKEAGVDGLIIVDLPPEHDAELATPAQAAGIDFIRLTTPTTDD
ARLPRVLERSSGFVYYVSVAGVTGAGSATTEHVTEAIARLRRHTDLPISVGFGIRTPEQAAAIARLADGVVVGSALVDKI
AQAKDAGQAVKDVLSLCSALADGVRAARV
>Mature_268_residues
SRLEQRFAELKAEGRSALVTFVTAGDPGYDASLEILKGLPAAGADVIELGMPFTDPMADGVAIQLATLRALEAGQTLAKT
LQMVREFRVGNQTTPIVLMGYYNPIHRFGVEQFVAEAKEAGVDGLIIVDLPPEHDAELATPAQAAGIDFIRLTTPTTDDA
RLPRVLERSSGFVYYVSVAGVTGAGSATTEHVTEAIARLRRHTDLPISVGFGIRTPEQAAAIARLADGVVVGSALVDKIA
QAKDAGQAVKDVLSLCSALADGVRAARV

Specific function: The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3- phosphate

COG id: COG0159

COG function: function code E; Tryptophan synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpA family

Homologues:

Organism=Escherichia coli, GI1787514, Length=243, Percent_Identity=38.6831275720165, Blast_Score=159, Evalue=1e-40,
Organism=Saccharomyces cerevisiae, GI6321412, Length=236, Percent_Identity=47.0338983050847, Blast_Score=214, Evalue=1e-56,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPA_PSEE4 (Q1IH21)

Other databases:

- EMBL:   CT573326
- RefSeq:   YP_605848.1
- ProteinModelPortal:   Q1IH21
- SMR:   Q1IH21
- STRING:   Q1IH21
- GeneID:   4087199
- GenomeReviews:   CT573326_GR
- KEGG:   pen:PSEEN0037
- NMPDR:   fig|384676.6.peg.32
- eggNOG:   COG0159
- HOGENOM:   HBG391690
- OMA:   NPIYIYG
- PhylomeDB:   Q1IH21
- ProtClustDB:   PRK13111
- BioCyc:   PENT384676:PSEEN0037-MONOMER
- HAMAP:   MF_00131
- InterPro:   IPR013785
- InterPro:   IPR011060
- InterPro:   IPR018204
- InterPro:   IPR002028
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00262

Pfam domain/function: PF00290 Trp_syntA; SSF51366 RibP_bind_barrel

EC number: =4.2.1.20

Molecular weight: Translated: 28307; Mature: 28175

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS00167 TRP_SYNTHASE_ALPHA

Important sites: ACT_SITE 49-49 ACT_SITE 60-60

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRLEQRFAELKAEGRSALVTFVTAGDPGYDASLEILKGLPAAGADVIELGMPFTDPMAD
CCHHHHHHHHHHHCCCEEEEEEEECCCCCCCHHHHHHHCCCCCCCHHHHCCCCCCCCCCC
GVAIQLATLRALEAGQTLAKTLQMVREFRVGNQTTPIVLMGYYNPIHRFGVEQFVAEAKE
CCEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHH
AGVDGLIIVDLPPEHDAELATPAQAAGIDFIRLTTPTTDDARLPRVLERSSGFVYYVSVA
CCCCCEEEEECCCCCCCCCCCCHHHCCCEEEEEECCCCCCHHHHHHHHHCCCEEEEEEEC
GVTGAGSATTEHVTEAIARLRRHTDLPISVGFGIRTPEQAAAIARLADGVVVGSALVDKI
CCCCCCCCHHHHHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHHHCCHHHHHHHHHHH
AQAKDAGQAVKDVLSLCSALADGVRAARV
HHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SRLEQRFAELKAEGRSALVTFVTAGDPGYDASLEILKGLPAAGADVIELGMPFTDPMAD
CHHHHHHHHHHHCCCEEEEEEEECCCCCCCHHHHHHHCCCCCCCHHHHCCCCCCCCCCC
GVAIQLATLRALEAGQTLAKTLQMVREFRVGNQTTPIVLMGYYNPIHRFGVEQFVAEAKE
CCEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHH
AGVDGLIIVDLPPEHDAELATPAQAAGIDFIRLTTPTTDDARLPRVLERSSGFVYYVSVA
CCCCCEEEEECCCCCCCCCCCCHHHCCCEEEEEECCCCCCHHHHHHHHHCCCEEEEEEEC
GVTGAGSATTEHVTEAIARLRRHTDLPISVGFGIRTPEQAAAIARLADGVVVGSALVDKI
CCCCCCCCHHHHHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHHHCCHHHHHHHHHHH
AQAKDAGQAVKDVLSLCSALADGVRAARV
HHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA