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The map label for this gene is glyS

Identifier: 104779330

GI number: 104779330

Start: 17494

End: 19548

Strand: Reverse

Name: glyS

Synonym: PSEEN0016

Alternate gene names: 104779330

Gene position: 19548-17494 (Counterclockwise)

Preceding gene: 104779331

Following gene: 162139702

Centisome position: 0.33

GC content: 65.94

Gene sequence:

>2055_bases
ATGAGTGCTCAAGATTTCCTGGTTGAACTGGGCACCGAAGAGCTGCCACCGAAGGCCCTGGCCTCGTTGGGCGATGCGTT
CCTCGCCGGCATCGAGAAAGGCTTGCAGGCCGCCGGCCTGAACTACACCAGCAAGCAGGTCTACGCCGCGCCGCGCCGCC
TGGCCGTGCTGCTGCGCCAGCTGGACGTGCAACAGCCGGATCGCAGCATCAACATCGACGGCCCGCCCCGCCAGGCGGCC
TTCGACGCCGAAGGCAACCCGACTCAAGCCGCCCTGGGCTTCGCCAAGAAGTGCGGCGTGGAGCTGTCGGACATCGACCA
GAGCGGCCCGAAGCTGCGCTTCTCGCAGCACATTCCGGGCAAGGCCACCGCCAGCCTGCTGCCCACCATCGTTGAGGATT
CGCTCAACGACCTGCCGATCCCCAAGCGCATGCACTGGGGCGCCAGCCGTGAAGAGTTCGTGCGCCCGACGCAATGGCTG
GTGATGCTGCTCGGCGACCAGGTCGTCGACTGCACCATCCTCGCCCAGAAAGCCGGCCGTGAATCCCGTGGCCACCGCTT
CCACCACCCTGAGAACGTGGTCATCACCACCCCGGCCAACTACGTCGAAGACCTGCGCAAAGCCTACGTGCTGGCCGACT
TCGCCGAGCGTCGCGAGCTGATCAGCAAGCGCACCGCGGAACTGGCCATGCAGCAGGAAGGCTCGGCCATCGTGCCGCCG
GCGCTGCTGGACGAAGTGACCGCCCTGGTCGAGTGGCCGGTGCCGCTGGTGTGCTCGTTCGAGGAGCGTTTCCTCGAGGT
GCCGCAGGAAGCCCTGATCACCACCATGCAGGACAACCAGAAGTACTTCTGCCTGCTGGACAGTGAAGGCAAGCTGCTGC
CGCGCTTCATCACCGTGGCCAACGTCGAGAGCCGCGATCCGAAGCAGATCGTCGAAGGCAACGAGAAGGTCGTGCGCCCA
CGCCTGACCGACGCCGAGTTCTTCTTCAAGCAAGACAAGAAGCAACCGCTGGAAAGCTTCAACGAGCGCCTGAAGAACGT
GGTGTTCCAGGCTCAGCTGGGCACCGTGTTCGACAAGGCCGAGCGCGTGTCGAAACTGGCTGCCTTCATTGCCCCGTACA
TCGGCGGCAGCGCCGCCAACGCTGGCCGTGCCGGCCTGCTGTCCAAGTGCGACCTGGCCTCGGAGATGGTCGGTGAATTC
CCTGAAATGCAGGGTATCGCCGGCTACTACTACGCCCTCAACGACGGTGAGCCGCAAGACGTCGCCCTGGCACTGAACGA
GCAGTACATGCCGCGCGGCGCTGGCGCCGAGCTGCCGCAAACCCTCACCGGTGCCGCCGTGGCCATCGCCGACAAGCTCG
ACACCCTGGTCGGCATCTTCGGCATCGGCATGCTGCCCACCGGCAGCAAGGATCCGTACGCCCTGCGCCGTGCCGCCCTG
GGCGTGCTGCGCATCCTGATCGAGAAACAGCTGGACCTGGACCTGACCACCGCGGTCGAGTTCGCGGTCAAGCAGTTCGG
CACCAAGGTGAAGGCTGCCGGCTTGTCCGAGCAGGTGCTGGAGTTCATCTTCGACCGCCTGCGCGCGCGTTACGAAGACG
AAGGCATCGACGTTGCCACCTACCTGTCGGTGCGTGCCCTGAAGCCGGGCTCGGCCCTGGACTTCGACCAGCGCGTACAG
GCCGTGCAGGCCTTCCGCAAGCTGCCGGAAGCCAATGCCCTGGCTGCGGCGAACAAGCGCGTGTCGAACCTGCTGGGCAA
GGCCGAAGGCGCCATCGCCGACCAGGTCGAGCCCAAGTACTTCGACAACGCCAACGAGTTCTCGCTGTACTCGGCCATCC
AGCAGGCCGACCAGGCCGTGCAGCCGATGGCCTCCGCGCGCCAGTACAACGAAGCCCTGGCCCGCCTGGCCGCCCTGCGC
GACCCGGTAGACGCCTTCTTCGAAGCGGTACTGGTCAACGCCGAGGACGCCAAGGTACGCGCCAACCGTTATGCCCTGCT
CAGCCGCCTGCGCGGCCTGTTCCTGGGCGTGGCCGATATCTCGCTGCTGGGGTAA

Upstream 100 bases:

>100_bases
ACGTGGCGCAAAGCTACCTGCAAGCCCGCGCACGCCTGGGCTTCCCGATGGCTTCTCCTGAACTGCGTGACGAAGTGTTG
GCTAAGCTGGAGGCTGCACA

Downstream 100 bases:

>100_bases
GTCGTTGAAACTGCTGATTCTTGATCGAGACGGAGTGATCAACCACGACTCCGACGCCTACATCAAGTCGCTGGAGGAGT
GGGTTCCCATTCCCGGCTCG

Product: glycyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Glycine--tRNA ligase beta subunit; GlyRS

Number of amino acids: Translated: 684; Mature: 683

Protein sequence:

>684_residues
MSAQDFLVELGTEELPPKALASLGDAFLAGIEKGLQAAGLNYTSKQVYAAPRRLAVLLRQLDVQQPDRSINIDGPPRQAA
FDAEGNPTQAALGFAKKCGVELSDIDQSGPKLRFSQHIPGKATASLLPTIVEDSLNDLPIPKRMHWGASREEFVRPTQWL
VMLLGDQVVDCTILAQKAGRESRGHRFHHPENVVITTPANYVEDLRKAYVLADFAERRELISKRTAELAMQQEGSAIVPP
ALLDEVTALVEWPVPLVCSFEERFLEVPQEALITTMQDNQKYFCLLDSEGKLLPRFITVANVESRDPKQIVEGNEKVVRP
RLTDAEFFFKQDKKQPLESFNERLKNVVFQAQLGTVFDKAERVSKLAAFIAPYIGGSAANAGRAGLLSKCDLASEMVGEF
PEMQGIAGYYYALNDGEPQDVALALNEQYMPRGAGAELPQTLTGAAVAIADKLDTLVGIFGIGMLPTGSKDPYALRRAAL
GVLRILIEKQLDLDLTTAVEFAVKQFGTKVKAAGLSEQVLEFIFDRLRARYEDEGIDVATYLSVRALKPGSALDFDQRVQ
AVQAFRKLPEANALAAANKRVSNLLGKAEGAIADQVEPKYFDNANEFSLYSAIQQADQAVQPMASARQYNEALARLAALR
DPVDAFFEAVLVNAEDAKVRANRYALLSRLRGLFLGVADISLLG

Sequences:

>Translated_684_residues
MSAQDFLVELGTEELPPKALASLGDAFLAGIEKGLQAAGLNYTSKQVYAAPRRLAVLLRQLDVQQPDRSINIDGPPRQAA
FDAEGNPTQAALGFAKKCGVELSDIDQSGPKLRFSQHIPGKATASLLPTIVEDSLNDLPIPKRMHWGASREEFVRPTQWL
VMLLGDQVVDCTILAQKAGRESRGHRFHHPENVVITTPANYVEDLRKAYVLADFAERRELISKRTAELAMQQEGSAIVPP
ALLDEVTALVEWPVPLVCSFEERFLEVPQEALITTMQDNQKYFCLLDSEGKLLPRFITVANVESRDPKQIVEGNEKVVRP
RLTDAEFFFKQDKKQPLESFNERLKNVVFQAQLGTVFDKAERVSKLAAFIAPYIGGSAANAGRAGLLSKCDLASEMVGEF
PEMQGIAGYYYALNDGEPQDVALALNEQYMPRGAGAELPQTLTGAAVAIADKLDTLVGIFGIGMLPTGSKDPYALRRAAL
GVLRILIEKQLDLDLTTAVEFAVKQFGTKVKAAGLSEQVLEFIFDRLRARYEDEGIDVATYLSVRALKPGSALDFDQRVQ
AVQAFRKLPEANALAAANKRVSNLLGKAEGAIADQVEPKYFDNANEFSLYSAIQQADQAVQPMASARQYNEALARLAALR
DPVDAFFEAVLVNAEDAKVRANRYALLSRLRGLFLGVADISLLG
>Mature_683_residues
SAQDFLVELGTEELPPKALASLGDAFLAGIEKGLQAAGLNYTSKQVYAAPRRLAVLLRQLDVQQPDRSINIDGPPRQAAF
DAEGNPTQAALGFAKKCGVELSDIDQSGPKLRFSQHIPGKATASLLPTIVEDSLNDLPIPKRMHWGASREEFVRPTQWLV
MLLGDQVVDCTILAQKAGRESRGHRFHHPENVVITTPANYVEDLRKAYVLADFAERRELISKRTAELAMQQEGSAIVPPA
LLDEVTALVEWPVPLVCSFEERFLEVPQEALITTMQDNQKYFCLLDSEGKLLPRFITVANVESRDPKQIVEGNEKVVRPR
LTDAEFFFKQDKKQPLESFNERLKNVVFQAQLGTVFDKAERVSKLAAFIAPYIGGSAANAGRAGLLSKCDLASEMVGEFP
EMQGIAGYYYALNDGEPQDVALALNEQYMPRGAGAELPQTLTGAAVAIADKLDTLVGIFGIGMLPTGSKDPYALRRAALG
VLRILIEKQLDLDLTTAVEFAVKQFGTKVKAAGLSEQVLEFIFDRLRARYEDEGIDVATYLSVRALKPGSALDFDQRVQA
VQAFRKLPEANALAAANKRVSNLLGKAEGAIADQVEPKYFDNANEFSLYSAIQQADQAVQPMASARQYNEALARLAALRD
PVDAFFEAVLVNAEDAKVRANRYALLSRLRGLFLGVADISLLG

Specific function: Unknown

COG id: COG0751

COG function: function code J; Glycyl-tRNA synthetase, beta subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Escherichia coli, GI1789982, Length=688, Percent_Identity=50.1453488372093, Blast_Score=639, Evalue=0.0,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 400 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYGB_PSEE4 (Q1IH28)

Other databases:

- EMBL:   CT573326
- RefSeq:   YP_605828.1
- ProteinModelPortal:   Q1IH28
- STRING:   Q1IH28
- GeneID:   4087396
- GenomeReviews:   CT573326_GR
- KEGG:   pen:PSEEN0016
- NMPDR:   fig|384676.6.peg.14
- eggNOG:   COG0751
- HOGENOM:   HBG586599
- OMA:   FIRPVHT
- PhylomeDB:   Q1IH28
- ProtClustDB:   PRK01233
- BioCyc:   PENT384676:PSEEN0016-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00255
- InterPro:   IPR008909
- InterPro:   IPR006194
- InterPro:   IPR015944
- InterPro:   IPR002311
- PRINTS:   PR01045
- TIGRFAMs:   TIGR00211

Pfam domain/function: PF05746 DALR_1; PF02092 tRNA_synt_2f

EC number: =6.1.1.14

Molecular weight: Translated: 74936; Mature: 74805

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: PS50861 AA_TRNA_LIGASE_II_GLYAB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAQDFLVELGTEELPPKALASLGDAFLAGIEKGLQAAGLNYTSKQVYAAPRRLAVLLRQ
CCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
LDVQQPDRSINIDGPPRQAAFDAEGNPTQAALGFAKKCGVELSDIDQSGPKLRFSQHIPG
HCCCCCCCEECCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHCCCCCCCEEECCCCCC
KATASLLPTIVEDSLNDLPIPKRMHWGASREEFVRPTQWLVMLLGDQVVDCTILAQKAGR
HHHHHHHHHHHHHCCCCCCCCCHHCCCCCHHHHHCHHHHHHHHHCCHHHHHHHHHHHCCC
ESRGHRFHHPENVVITTPANYVEDLRKAYVLADFAERRELISKRTAELAMQQEGSAIVPP
CCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCH
ALLDEVTALVEWPVPLVCSFEERFLEVPQEALITTMQDNQKYFCLLDSEGKLLPRFITVA
HHHHHHHHHHHCCCCCEECHHHHHHHCCHHHHHHHHCCCCEEEEEECCCCCCCHHHHEEE
NVESRDPKQIVEGNEKVVRPRLTDAEFFFKQDKKQPLESFNERLKNVVFQAQLGTVFDKA
CCCCCCHHHHHCCCCCEECCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
ERVSKLAAFIAPYIGGSAANAGRAGLLSKCDLASEMVGEFPEMQGIAGYYYALNDGEPQD
HHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCC
VALALNEQYMPRGAGAELPQTLTGAAVAIADKLDTLVGIFGIGMLPTGSKDPYALRRAAL
EEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
GVLRILIEKQLDLDLTTAVEFAVKQFGTKVKAAGLSEQVLEFIFDRLRARYEDEGIDVAT
HHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHHHCCCCCCEEH
YLSVRALKPGSALDFDQRVQAVQAFRKLPEANALAAANKRVSNLLGKAEGAIADQVEPKY
HHEEEEECCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHCCCCCC
FDNANEFSLYSAIQQADQAVQPMASARQYNEALARLAALRDPVDAFFEAVLVNAEDAKVR
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCHHHH
ANRYALLSRLRGLFLGVADISLLG
HHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SAQDFLVELGTEELPPKALASLGDAFLAGIEKGLQAAGLNYTSKQVYAAPRRLAVLLRQ
CHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
LDVQQPDRSINIDGPPRQAAFDAEGNPTQAALGFAKKCGVELSDIDQSGPKLRFSQHIPG
HCCCCCCCEECCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHCCCCCCCEEECCCCCC
KATASLLPTIVEDSLNDLPIPKRMHWGASREEFVRPTQWLVMLLGDQVVDCTILAQKAGR
HHHHHHHHHHHHHCCCCCCCCCHHCCCCCHHHHHCHHHHHHHHHCCHHHHHHHHHHHCCC
ESRGHRFHHPENVVITTPANYVEDLRKAYVLADFAERRELISKRTAELAMQQEGSAIVPP
CCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCH
ALLDEVTALVEWPVPLVCSFEERFLEVPQEALITTMQDNQKYFCLLDSEGKLLPRFITVA
HHHHHHHHHHHCCCCCEECHHHHHHHCCHHHHHHHHCCCCEEEEEECCCCCCCHHHHEEE
NVESRDPKQIVEGNEKVVRPRLTDAEFFFKQDKKQPLESFNERLKNVVFQAQLGTVFDKA
CCCCCCHHHHHCCCCCEECCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
ERVSKLAAFIAPYIGGSAANAGRAGLLSKCDLASEMVGEFPEMQGIAGYYYALNDGEPQD
HHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCC
VALALNEQYMPRGAGAELPQTLTGAAVAIADKLDTLVGIFGIGMLPTGSKDPYALRRAAL
EEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
GVLRILIEKQLDLDLTTAVEFAVKQFGTKVKAAGLSEQVLEFIFDRLRARYEDEGIDVAT
HHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHHHCCCCCCEEH
YLSVRALKPGSALDFDQRVQAVQAFRKLPEANALAAANKRVSNLLGKAEGAIADQVEPKY
HHEEEEECCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHCCCCCC
FDNANEFSLYSAIQQADQAVQPMASARQYNEALARLAALRDPVDAFFEAVLVNAEDAKVR
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCHHHH
ANRYALLSRLRGLFLGVADISLLG
HHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA