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The map label for this gene is amiC [H]

Identifier: 103488648

GI number: 103488648

Start: 3329511

End: 3330578

Strand: Direct

Name: amiC [H]

Synonym: Sala_3173

Alternate gene names: 103488648

Gene position: 3329511-3330578 (Clockwise)

Preceding gene: 103488641

Following gene: 103488649

Centisome position: 99.53

GC content: 66.48

Gene sequence:

>1068_bases
ATGGGATCGCCGTTCACAGTTGCAAGAATGTCATGCGTGGCCGGTGCCGCCTTTCGCATAAAAGGCCGTCGTCGCTGCGC
CATCTTCCATGCTCGATTCAATCTCGCAGGGCGCTGGAATCGGGTCATTTCGAATAAGCGCTGGACCAATCGCCACGTCG
GACGGCATAGACGCGCCATGATGCTGCTCGCCCTTATCGGCCTGATCGCGACGCCGCCGACGGGGGGCGCCGCAGCCGAT
CGCCCGCGCGCCGAGCATCCTGTCACCCTTGCGTTTCGCGCGCGGCGACCGGCCCGGCGCTACAGCGTCACCGTTCCGAT
CGGCAAGCCCCGGCCCGCGGTGCCGCTGCCGCGGATCGAGGGACCCGACGATAATCGCCTGCCGCTCGTGGTGATCGACG
CGGGGCACGGCGGGCACGACCCGGGCGCGATTTCGCCGCACAATGGTCGGCGTGAAAAGGACGTCACATTGGCGATCGCG
CGCGCGATCCGCGACGAACTCCTCGCCTCGCGCCGCGTCCGCGTCGCGCTCACCCGCGACGACGACCGCTTCCTTGTGCT
CGAGGAACGCTATGGCATCGCACGGCGGCTGAAGGCCGACCTCTTCATTTCGGTCCACGCCGATGCTGCCGAGAACAGCG
AGGCGCACGGCGCGTCGGTCTATACCTTGTCCGAAGTCGCATCCGACCGCGAGGCGGCACGGCTTGCGGCGCGCGAGAAC
AAGGCGAATATCATCAACGGCGTCGATCTGGGGGCGCATGGCGATGCCGTGTCGTCGATCCTTATCGACCTGACCCAGCG
CGAAACGATGAACGCTTCGGCCGAATTTGCCCGCCTGCTCCAGCGCGAGGCGTCGGACGAGATGACATTCCGCACCACCG
CGCACCGCTTTGCCTCCTTCGTCGTGCTGAAGGCACCCGATACGCCGTCCGTGCTGTTCGAGACGGGTTTTCTCTCGAAC
AAGGGTGACGCCGCATTTCTCGCCTCGGCGTCGGGGCGCAAAAAGGTGGCGCGCGGCGTGCGCGAAGCGGTGCAGATCCA
TTTCGCGCGGCGTCTTGCGGCCGAGTGA

Upstream 100 bases:

>100_bases
GCCTTCCCCTCAAAAGGCCGGACGGCACCGGAAAGGACGCCGTCAGTGATGGGGTGCTAGCATCGGCACGGGCCGACGGC
AACCACAACCGCTGCCAAAG

Downstream 100 bases:

>100_bases
CGGCAGCCTTGTCGGGAGCGAAGGAGGGAGCGGCATGAGCTGGAGCAACTGGTCGGGCAGCGTCGCCGCGCGCGCCGCGA
TAGCGCGCCCGCAAAGCGAG

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 355; Mature: 354

Protein sequence:

>355_residues
MGSPFTVARMSCVAGAAFRIKGRRRCAIFHARFNLAGRWNRVISNKRWTNRHVGRHRRAMMLLALIGLIATPPTGGAAAD
RPRAEHPVTLAFRARRPARRYSVTVPIGKPRPAVPLPRIEGPDDNRLPLVVIDAGHGGHDPGAISPHNGRREKDVTLAIA
RAIRDELLASRRVRVALTRDDDRFLVLEERYGIARRLKADLFISVHADAAENSEAHGASVYTLSEVASDREAARLAAREN
KANIINGVDLGAHGDAVSSILIDLTQRETMNASAEFARLLQREASDEMTFRTTAHRFASFVVLKAPDTPSVLFETGFLSN
KGDAAFLASASGRKKVARGVREAVQIHFARRLAAE

Sequences:

>Translated_355_residues
MGSPFTVARMSCVAGAAFRIKGRRRCAIFHARFNLAGRWNRVISNKRWTNRHVGRHRRAMMLLALIGLIATPPTGGAAAD
RPRAEHPVTLAFRARRPARRYSVTVPIGKPRPAVPLPRIEGPDDNRLPLVVIDAGHGGHDPGAISPHNGRREKDVTLAIA
RAIRDELLASRRVRVALTRDDDRFLVLEERYGIARRLKADLFISVHADAAENSEAHGASVYTLSEVASDREAARLAAREN
KANIINGVDLGAHGDAVSSILIDLTQRETMNASAEFARLLQREASDEMTFRTTAHRFASFVVLKAPDTPSVLFETGFLSN
KGDAAFLASASGRKKVARGVREAVQIHFARRLAAE
>Mature_354_residues
GSPFTVARMSCVAGAAFRIKGRRRCAIFHARFNLAGRWNRVISNKRWTNRHVGRHRRAMMLLALIGLIATPPTGGAAADR
PRAEHPVTLAFRARRPARRYSVTVPIGKPRPAVPLPRIEGPDDNRLPLVVIDAGHGGHDPGAISPHNGRREKDVTLAIAR
AIRDELLASRRVRVALTRDDDRFLVLEERYGIARRLKADLFISVHADAAENSEAHGASVYTLSEVASDREAARLAARENK
ANIINGVDLGAHGDAVSSILIDLTQRETMNASAEFARLLQREASDEMTFRTTAHRFASFVVLKAPDTPSVLFETGFLSNK
GDAAFLASASGRKKVARGVREAVQIHFARRLAAE

Specific function: Cell-wall hydrolase involved in septum cleavage during cell division [H]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI1790611, Length=281, Percent_Identity=35.5871886120996, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI1788776, Length=221, Percent_Identity=38.4615384615385, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI87082163, Length=242, Percent_Identity=34.7107438016529, Blast_Score=132, Evalue=4e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR021731
- InterPro:   IPR002508 [H]

Pfam domain/function: PF01520 Amidase_3; PF11741 AMIN [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 38927; Mature: 38796

Theoretical pI: Translated: 11.68; Mature: 11.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGSPFTVARMSCVAGAAFRIKGRRRCAIFHARFNLAGRWNRVISNKRWTNRHVGRHRRAM
CCCCCHHHHHHHHHCCEEEECCCCEEEEEEEEECCCHHHHHHHCCCCCCHHHHHHHHHHH
MLLALIGLIATPPTGGAAADRPRAEHPVTLAFRARRPARRYSVTVPIGKPRPAVPLPRIE
HHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCEEEEEECCCCCCCCCCCCCC
GPDDNRLPLVVIDAGHGGHDPGAISPHNGRREKDVTLAIARAIRDELLASRRVRVALTRD
CCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEC
DDRFLVLEERYGIARRLKADLFISVHADAAENSEAHGASVYTLSEVASDREAARLAAREN
CCCEEEEECCCCHHHHHCEEEEEEEECCCCCCCCCCCCEEEEHHHHHCHHHHHHHHHHCC
KANIINGVDLGAHGDAVSSILIDLTQRETMNASAEFARLLQREASDEMTFRTTAHRFASF
CCCEECCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHEEE
VVLKAPDTPSVLFETGFLSNKGDAAFLASASGRKKVARGVREAVQIHFARRLAAE
EEEECCCCCCEEEEECCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
GSPFTVARMSCVAGAAFRIKGRRRCAIFHARFNLAGRWNRVISNKRWTNRHVGRHRRAM
CCCCHHHHHHHHHCCEEEECCCCEEEEEEEEECCCHHHHHHHCCCCCCHHHHHHHHHHH
MLLALIGLIATPPTGGAAADRPRAEHPVTLAFRARRPARRYSVTVPIGKPRPAVPLPRIE
HHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCEEEEEECCCCCCCCCCCCCC
GPDDNRLPLVVIDAGHGGHDPGAISPHNGRREKDVTLAIARAIRDELLASRRVRVALTRD
CCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEC
DDRFLVLEERYGIARRLKADLFISVHADAAENSEAHGASVYTLSEVASDREAARLAAREN
CCCEEEEECCCCHHHHHCEEEEEEEECCCCCCCCCCCCEEEEHHHHHCHHHHHHHHHHCC
KANIINGVDLGAHGDAVSSILIDLTQRETMNASAEFARLLQREASDEMTFRTTAHRFASF
CCCEECCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHEEE
VVLKAPDTPSVLFETGFLSNKGDAAFLASASGRKKVARGVREAVQIHFARRLAAE
EEEECCCCCCEEEEECCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10710307 [H]