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The map label for this gene is rne [H]

Identifier: 103488647

GI number: 103488647

Start: 3326431

End: 3329076

Strand: Reverse

Name: rne [H]

Synonym: Sala_3172

Alternate gene names: 103488647

Gene position: 3329076-3326431 (Counterclockwise)

Preceding gene: 103488654

Following gene: 103488646

Centisome position: 99.52

GC content: 67.5

Gene sequence:

>2646_bases
ATGGCGCGCTGCGGCGCGCTTGGAGTGTTACCAATGACCACGCGCATGTTGATCGACGCGCGGCACCGGGAAGAAACCCG
GGTCGCCGTCACCAAGGGAAACCGCATCGAGGAGTTTGATTTCGAGTCCGCCGAGCACAAGCAGCTCAAGGGAAATATCT
ATCTGGCCAAGGTAACCCGCGTCGAACCGTCGCTGCAGGCGGCGTTCGTCGAATATGGGGGCAACCGCCACGGATTCCTG
GCATTCAGCGAAATCCACCCCGATTATTACCAGATTCCCAAGGAAGACCGCGAAGCATTGCTGCGCGAAGAGGCCGAACA
TGCCGCCGCCGCGGCGCAGCGTGCCGAGGACGATCTCGACGAGCTGGAGGGCGATGTCGCCGACGTCGAGGAGCATGACG
ACGAAAATGGCGACGCCCCCGCGCCCGAAACGATCGGCGAAGGCGAAGGCGAGGACGACGGCGAGAATGGCGATGCCGAC
AATGGCGACGCCGATGGCGAAGAGGGCGCCGAGGAGGGCAGGGAAAACCGCGGCGACCGCAAGCGCCGCGGGCGCGACGG
GCGCAAGGGCGGCCGGGGCCGCGGCCGACGCAGCGACGAGGAGGATGGCGAAAGCCGCATGTCGCTGCGCCGCCGCTACA
AGATCCAGGACGTCATCCGCCGCCGCCAGGTGATGCTGGTGCAGGTCGTCAAGGAAGAGCGCGGCAACAAGGGGGCGGCG
CTCACCACCTATCTGTCGCTGGCCGGCCGCTACTGCGTGCTGATGCCCAACACGATGCACGGTGGCGGTATCAGCCGCAA
GATTTCGAACGGCGCCGATCGCCGCAAGCTGAAGGCGATGATCGACGAGATGAACCTGCCGCCGACGATGGGCTGCATCG
TTCGCACGGCGGGCATGAGCCGTACCAAGACCGAGATCAAGCGCGACTTCGACTATCTCGCGCGCCTGTGGGACGAGATT
CGCGAAAAGACGCTGGCGTCGAGCGCGCCCGCGCTCATCCATTCGGACAGCGACCTGATCAAACGCGCGATCCGCGACAT
TTATCACAAGGACATCGAGGAAGTGCTCGTCGAGGGCGACGAGGGCTACAAGGCGGCGAAGCAGTTCATGAAGCTCTTGA
TGCCGAGCCACGCGCGGCGCGTGAAGCAATATGCCGATCCCGTCTCGCTCTATCAGCGCTACGGCGTCGAAGACCAGCTT
GCCGGGATGCTCAATCCCGTCGTCCAGCTGAAATCGGGCGGCTATCTGGTGATCAACCCGACCGAGGCGCTGGTGTCGAT
CGACATCAACTCGGGCCGGTCGACGCGCGAGCATAATATCGAACAGACGGCGCTCAGCACCAATCTGGAGGCCGCGGCCG
AAATCGCGCGCCAGCTGCGCCTGCGCGACATGGCGGGGCTGATCGTCATCGACTTCATCGACATGGATCATGGCTCGAAC
GTCCGCAAGGTCGAGCGCGCGATGAAGGAGGCGCTGAAGAACGATCGCGCGCGCATCCAGGTCGGCCGCATTTCGGGTTT
CGGCCTGATGGAAATGAGCCGCCAGCGGCTGCGCACCGGGGTGCTCGAAGCCTCGACGCGGCCCTGTCCGCATTGCGAGG
GCACCGGGCTCGTCCGCACTGCATCGTCGGCGGGGCTCAGCGCGCTGCGCCTGATCGAAGAGGAAGCGGCGCGCGGCAAG
GGCAGCAAGATCACGCTGCGTGCGAGCCAGGAAGCGACCTTCTACCTGCTCAACGAAAAGCGCCGCGAACTGCACGAAAT
CGAGGAGCTTTACGGCGTCAGCGTCGAAATCCTGCCGGACGGCGAGACCGAGGGCGCGCGCATGGCGATCGAGGTCGGCG
GCCCGCCGCCCACCCAGCGCCGCAGCTTCGCCCCGATCGAGCCGATCGAGGATGATGAGGACGACATCCCCGAAGAGGAG
GATGAAGAGGAAAGCGACGAGGACGCATCGGACAGCCGCGCGCCCGCGCGGGCCCGTGACGAAAGCGACACCGATGGCGA
GGGCCGCAAGCGCCGCCGCCGTCGTCGCCGCAGCCGCCGCGGTCGCCGCGACGAGGAAGGCACCGAAGGCGCCGAGGATG
CGCCCTCCGCGGTGGACGATGTCCAGGAAACGGCAGGCGACGCCGAAGCCGCGTCGACGACCGACGAGGCACCGGGAACC
GATGCCGAGGCCGAACCTGCCGCGAAAAAACCGCGCCGTCGCGGCGGACGCGGGCGCAAGAAGGGCGACGAGGCCAATAG
CGCAACCGACGCCGACGCAGCGACCGCCGAACCGGCACCGGAGCCCGAAGCGGCGACCGACGCGCCGCTGCCGCAAGCAG
CCGACGCAACGGTCGAGGCAGTCGCGGCCGAAGACAAGCCGAAACGCAAACGCGCACCGCGCAAGACCAAGGCGGCGCTG
GCCGCCGAGGCTGCAGCGGAGGCCGAAGCGGCCGAAGCCCCCGTCACCGACGCCCCTGCCGAAGCCGCCGAACCCGCGCC
GAAGAAGCGCGCGAGCCGAGCGAAAAAGGCCGTCGCAGCCGAAGCGACCAGCGAAGCTGCGACCCAAAGCGCCGCCGAAC
CGGTCGCCGCCGACGCGGGCGAGACCGGCGAGGCGGCGGATGGCGAACCGCGCCGCGGCTGGTGGCAGCGCACGTTCGGC
AATTGA

Upstream 100 bases:

>100_bases
GTCCAGGACCGGACGCAAGCCGCCTTCGGCAATAACGATTTTTGTCTTTTCACCCTTATCCGCCGCGACTCTCCGCCAGG
AGTGTCTGCGGGATTTTTTG

Downstream 100 bases:

>100_bases
TCGTCCCGACCGATCGGGCGACAATCGGTCGCGGAAATCGCACGCCGACCTTGCGCCTGCGCGCGCTGACCCCCAATAAG
CCCTCATGGCTTCACTCGGG

Product: ribonuclease

Products: NA

Alternate protein names: RNase E [H]

Number of amino acids: Translated: 881; Mature: 880

Protein sequence:

>881_residues
MARCGALGVLPMTTRMLIDARHREETRVAVTKGNRIEEFDFESAEHKQLKGNIYLAKVTRVEPSLQAAFVEYGGNRHGFL
AFSEIHPDYYQIPKEDREALLREEAEHAAAAAQRAEDDLDELEGDVADVEEHDDENGDAPAPETIGEGEGEDDGENGDAD
NGDADGEEGAEEGRENRGDRKRRGRDGRKGGRGRGRRSDEEDGESRMSLRRRYKIQDVIRRRQVMLVQVVKEERGNKGAA
LTTYLSLAGRYCVLMPNTMHGGGISRKISNGADRRKLKAMIDEMNLPPTMGCIVRTAGMSRTKTEIKRDFDYLARLWDEI
REKTLASSAPALIHSDSDLIKRAIRDIYHKDIEEVLVEGDEGYKAAKQFMKLLMPSHARRVKQYADPVSLYQRYGVEDQL
AGMLNPVVQLKSGGYLVINPTEALVSIDINSGRSTREHNIEQTALSTNLEAAAEIARQLRLRDMAGLIVIDFIDMDHGSN
VRKVERAMKEALKNDRARIQVGRISGFGLMEMSRQRLRTGVLEASTRPCPHCEGTGLVRTASSAGLSALRLIEEEAARGK
GSKITLRASQEATFYLLNEKRRELHEIEELYGVSVEILPDGETEGARMAIEVGGPPPTQRRSFAPIEPIEDDEDDIPEEE
DEEESDEDASDSRAPARARDESDTDGEGRKRRRRRRRSRRGRRDEEGTEGAEDAPSAVDDVQETAGDAEAASTTDEAPGT
DAEAEPAAKKPRRRGGRGRKKGDEANSATDADAATAEPAPEPEAATDAPLPQAADATVEAVAAEDKPKRKRAPRKTKAAL
AAEAAAEAEAAEAPVTDAPAEAAEPAPKKRASRAKKAVAAEATSEAATQSAAEPVAADAGETGEAADGEPRRGWWQRTFG
N

Sequences:

>Translated_881_residues
MARCGALGVLPMTTRMLIDARHREETRVAVTKGNRIEEFDFESAEHKQLKGNIYLAKVTRVEPSLQAAFVEYGGNRHGFL
AFSEIHPDYYQIPKEDREALLREEAEHAAAAAQRAEDDLDELEGDVADVEEHDDENGDAPAPETIGEGEGEDDGENGDAD
NGDADGEEGAEEGRENRGDRKRRGRDGRKGGRGRGRRSDEEDGESRMSLRRRYKIQDVIRRRQVMLVQVVKEERGNKGAA
LTTYLSLAGRYCVLMPNTMHGGGISRKISNGADRRKLKAMIDEMNLPPTMGCIVRTAGMSRTKTEIKRDFDYLARLWDEI
REKTLASSAPALIHSDSDLIKRAIRDIYHKDIEEVLVEGDEGYKAAKQFMKLLMPSHARRVKQYADPVSLYQRYGVEDQL
AGMLNPVVQLKSGGYLVINPTEALVSIDINSGRSTREHNIEQTALSTNLEAAAEIARQLRLRDMAGLIVIDFIDMDHGSN
VRKVERAMKEALKNDRARIQVGRISGFGLMEMSRQRLRTGVLEASTRPCPHCEGTGLVRTASSAGLSALRLIEEEAARGK
GSKITLRASQEATFYLLNEKRRELHEIEELYGVSVEILPDGETEGARMAIEVGGPPPTQRRSFAPIEPIEDDEDDIPEEE
DEEESDEDASDSRAPARARDESDTDGEGRKRRRRRRRSRRGRRDEEGTEGAEDAPSAVDDVQETAGDAEAASTTDEAPGT
DAEAEPAAKKPRRRGGRGRKKGDEANSATDADAATAEPAPEPEAATDAPLPQAADATVEAVAAEDKPKRKRAPRKTKAAL
AAEAAAEAEAAEAPVTDAPAEAAEPAPKKRASRAKKAVAAEATSEAATQSAAEPVAADAGETGEAADGEPRRGWWQRTFG
N
>Mature_880_residues
ARCGALGVLPMTTRMLIDARHREETRVAVTKGNRIEEFDFESAEHKQLKGNIYLAKVTRVEPSLQAAFVEYGGNRHGFLA
FSEIHPDYYQIPKEDREALLREEAEHAAAAAQRAEDDLDELEGDVADVEEHDDENGDAPAPETIGEGEGEDDGENGDADN
GDADGEEGAEEGRENRGDRKRRGRDGRKGGRGRGRRSDEEDGESRMSLRRRYKIQDVIRRRQVMLVQVVKEERGNKGAAL
TTYLSLAGRYCVLMPNTMHGGGISRKISNGADRRKLKAMIDEMNLPPTMGCIVRTAGMSRTKTEIKRDFDYLARLWDEIR
EKTLASSAPALIHSDSDLIKRAIRDIYHKDIEEVLVEGDEGYKAAKQFMKLLMPSHARRVKQYADPVSLYQRYGVEDQLA
GMLNPVVQLKSGGYLVINPTEALVSIDINSGRSTREHNIEQTALSTNLEAAAEIARQLRLRDMAGLIVIDFIDMDHGSNV
RKVERAMKEALKNDRARIQVGRISGFGLMEMSRQRLRTGVLEASTRPCPHCEGTGLVRTASSAGLSALRLIEEEAARGKG
SKITLRASQEATFYLLNEKRRELHEIEELYGVSVEILPDGETEGARMAIEVGGPPPTQRRSFAPIEPIEDDEDDIPEEED
EEESDEDASDSRAPARARDESDTDGEGRKRRRRRRRSRRGRRDEEGTEGAEDAPSAVDDVQETAGDAEAASTTDEAPGTD
AEAEPAAKKPRRRGGRGRKKGDEANSATDADAATAEPAPEPEAATDAPLPQAADATVEAVAAEDKPKRKRAPRKTKAALA
AEAAAEAEAAEAPVTDAPAEAAEPAPKKRASRAKKAVAAEATSEAATQSAAEPVAADAGETGEAADGEPRRGWWQRTFGN

Specific function: Matures 5S rRNA from its precursors from all the rRNA genes. It also cleaves RNA I, a molecule that controls the replication of colE1 plasmid DNA. It is the major endoribonuclease participating in mRNA turnover in E.coli. It initiates the decay of RNAs by

COG id: COG1530

COG function: function code J; Ribonucleases G and E

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1787325, Length=395, Percent_Identity=47.3417721518987, Blast_Score=347, Evalue=2e-96,
Organism=Escherichia coli, GI87082235, Length=387, Percent_Identity=33.0749354005168, Blast_Score=192, Evalue=6e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR021968
- InterPro:   IPR003029
- InterPro:   IPR019307
- InterPro:   IPR022967
- InterPro:   IPR004659 [H]

Pfam domain/function: PF12111 PNPase_C; PF10150 RNase_E_G; PF00575 S1 [H]

EC number: =3.1.26.12 [H]

Molecular weight: Translated: 96477; Mature: 96346

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARCGALGVLPMTTRMLIDARHREETRVAVTKGNRIEEFDFESAEHKQLKGNIYLAKVTR
CCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCHHHHHHCCCEEEEEEEC
VEPSLQAAFVEYGGNRHGFLAFSEIHPDYYQIPKEDREALLREEAEHAAAAAQRAEDDLD
CCHHHHHHHHHHCCCCCCEEEECCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELEGDVADVEEHDDENGDAPAPETIGEGEGEDDGENGDADNGDADGEEGAEEGRENRGDR
HHCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCHH
KRRGRDGRKGGRGRGRRSDEEDGESRMSLRRRYKIQDVIRRRQVMLVQVVKEERGNKGAA
HHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
LTTYLSLAGRYCVLMPNTMHGGGISRKISNGADRRKLKAMIDEMNLPPTMGCIVRTAGMS
HHHHHHHHCCEEEEECCCCCCCCCHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHCCCC
RTKTEIKRDFDYLARLWDEIREKTLASSAPALIHSDSDLIKRAIRDIYHKDIEEVLVEGD
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHHHHHCCC
EGYKAAKQFMKLLMPSHARRVKQYADPVSLYQRYGVEDQLAGMLNPVVQLKSGGYLVINP
CCHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHEECCCCEEEECC
TEALVSIDINSGRSTREHNIEQTALSTNLEAAAEIARQLRLRDMAGLIVIDFIDMDHGSN
CCEEEEEECCCCCCHHHHCHHHHHHHCCHHHHHHHHHHHHHHHHCCEEEEEEEECCCCCC
VRKVERAMKEALKNDRARIQVGRISGFGLMEMSRQRLRTGVLEASTRPCPHCEGTGLVRT
HHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEE
ASSAGLSALRLIEEEAARGKGSKITLRASQEATFYLLNEKRRELHEIEELYGVSVEILPD
CCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCEEEEHHHHHHHHHHHHHHHCCEEEEECC
GETEGARMAIEVGGPPPTQRRSFAPIEPIEDDEDDIPEEEDEEESDEDASDSRAPARARD
CCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
ESDTDGEGRKRRRRRRRSRRGRRDEEGTEGAEDAPSAVDDVQETAGDAEAASTTDEAPGT
CCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCCCCHHCCCCCCCCCC
DAEAEPAAKKPRRRGGRGRKKGDEANSATDADAATAEPAPEPEAATDAPLPQAADATVEA
CCCCCCCHHCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHH
VAAEDKPKRKRAPRKTKAALAAEAAAEAEAAEAPVTDAPAEAAEPAPKKRASRAKKAVAA
HHCCCCCHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHCCCCHHHHHHHHHHHHHH
EATSEAATQSAAEPVAADAGETGEAADGEPRRGWWQRTFGN
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHCCCC
>Mature Secondary Structure 
ARCGALGVLPMTTRMLIDARHREETRVAVTKGNRIEEFDFESAEHKQLKGNIYLAKVTR
CCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCHHHHHHCCCEEEEEEEC
VEPSLQAAFVEYGGNRHGFLAFSEIHPDYYQIPKEDREALLREEAEHAAAAAQRAEDDLD
CCHHHHHHHHHHCCCCCCEEEECCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELEGDVADVEEHDDENGDAPAPETIGEGEGEDDGENGDADNGDADGEEGAEEGRENRGDR
HHCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCHH
KRRGRDGRKGGRGRGRRSDEEDGESRMSLRRRYKIQDVIRRRQVMLVQVVKEERGNKGAA
HHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
LTTYLSLAGRYCVLMPNTMHGGGISRKISNGADRRKLKAMIDEMNLPPTMGCIVRTAGMS
HHHHHHHHCCEEEEECCCCCCCCCHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHCCCC
RTKTEIKRDFDYLARLWDEIREKTLASSAPALIHSDSDLIKRAIRDIYHKDIEEVLVEGD
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHHHHHCCC
EGYKAAKQFMKLLMPSHARRVKQYADPVSLYQRYGVEDQLAGMLNPVVQLKSGGYLVINP
CCHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHEECCCCEEEECC
TEALVSIDINSGRSTREHNIEQTALSTNLEAAAEIARQLRLRDMAGLIVIDFIDMDHGSN
CCEEEEEECCCCCCHHHHCHHHHHHHCCHHHHHHHHHHHHHHHHCCEEEEEEEECCCCCC
VRKVERAMKEALKNDRARIQVGRISGFGLMEMSRQRLRTGVLEASTRPCPHCEGTGLVRT
HHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEE
ASSAGLSALRLIEEEAARGKGSKITLRASQEATFYLLNEKRRELHEIEELYGVSVEILPD
CCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCEEEEHHHHHHHHHHHHHHHCCEEEEECC
GETEGARMAIEVGGPPPTQRRSFAPIEPIEDDEDDIPEEEDEEESDEDASDSRAPARARD
CCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
ESDTDGEGRKRRRRRRRSRRGRRDEEGTEGAEDAPSAVDDVQETAGDAEAASTTDEAPGT
CCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCCCCHHCCCCCCCCCC
DAEAEPAAKKPRRRGGRGRKKGDEANSATDADAATAEPAPEPEAATDAPLPQAADATVEA
CCCCCCCHHCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHH
VAAEDKPKRKRAPRKTKAALAAEAAAEAEAAEAPVTDAPAEAAEPAPKKRASRAKKAVAA
HHCCCCCHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHCCCCHHHHHHHHHHHHHH
EATSEAATQSAAEPVAADAGETGEAADGEPRRGWWQRTFGN
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 1447789; 1704367; 2011493; 8415644 [H]