BacMap

Definition	Sphingopyxis alaskensis RB2256, complete genome.
Accession	NC_008048
Length	3,345,170

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The map label for this gene is rhlG [H]

Identifier: 103488555

GI number: 103488555

Start: 3230783

End: 3231586

Strand: Direct

Name: rhlG [H]

Synonym: Sala_3079

Alternate gene names: 103488555

Gene position: 3230783-3231586 (Clockwise)

Preceding gene: 103488554

Following gene: 103488556

Centisome position: 96.58

GC content: 64.8

Gene sequence:

>804_bases
ATGAACCTCACTGACATGTTCGGCCTCGACGGCCGCATCGCCCTCGTCACCGGCGGCTCGCGCGGCATCGGCAAGATGAT
CGTCGAGGGCTATCTCGCCGCGGGCGCCGCGCGCGTTTATATCTCGGCGCGCAAGACCGCGCAGATTGAGGAAGCCGTCG
CCGATTTCGAAACGCGCTATCCGGGCAAGGTCATCGGCCTACCCGTAGACCTCTCGACGGTCGAGGGCTGCCGCGCGCTC
GCCAGAGAACTCGAAGCGCGCGAGGAGCGCCTCGACATCCTCGTCAACAATGCCGGCGCGGCGTGGGGCGAACCCTTCGA
GGGCTTTCCCGAAGCGGGCTGGGACAAGGTGATGGACATCAACGTCAAATCGCCCTTCTTCCTGACGCAGGCGCTGCATG
GGTTACTCAAGGCTGCGGGCACGCACGAACGCCCCGCCAAGGTCATCAACATCGGCTCGATCGACGGGATGCGCCTCAAT
CCGTGGGAAACCTACAGCTATCATGCGTCGAAAGCCGCAATCCTCTATCTCACCAAGCGCCTCGCGGCGCGCCTCGTCAC
CGACCATATCCTCGTCACCGCGATTGCGCCGGGCGCCTTCCAGTCGGACATGAACAAGGCGGCGCGCGACCATGGCGACG
CGGTCGCGAAGAGCATTCCGGTGAAACGCATCGGCGTGCCCGAGGATATGGCCGGCGCTGCGATCTATCTCGCGTCGAAG
GCGGGCGATTATGTGGTGGGCGACACGATCACGGTCGACGGCGGGCTGGTCCACGGCGATCTGAAGACCAGCATCGACGC
CTGA

Upstream 100 bases:

>100_bases
CTTAGTCGCAGCGGGTGGGGGCCAGAGGCCTTGCGCTACGCCGCGAGCCCCCACCCCCAACCCCTCCCCTGAAGGGGAGG
GGCTTAGAAAGGATAACGCC

Downstream 100 bases:

>100_bases
TCCAAGCCCCTCCCTTTCAGCGGAGGGCGTGGACCAATTGACTCGTTCATCGCCAAGGTGTATTATTCAACTAATGCACC
AAGGAGACGTGTCATGCGTT

Product: short-chain dehydrogenase/reductase SDR

Products: NA

Alternate protein names: 3-ketoacyl-acyl carrier protein reductase [H]

Number of amino acids: Translated: 267; Mature: 267

Protein sequence:

>267_residues
MNLTDMFGLDGRIALVTGGSRGIGKMIVEGYLAAGAARVYISARKTAQIEEAVADFETRYPGKVIGLPVDLSTVEGCRAL
ARELEAREERLDILVNNAGAAWGEPFEGFPEAGWDKVMDINVKSPFFLTQALHGLLKAAGTHERPAKVINIGSIDGMRLN
PWETYSYHASKAAILYLTKRLAARLVTDHILVTAIAPGAFQSDMNKAARDHGDAVAKSIPVKRIGVPEDMAGAAIYLASK
AGDYVVGDTITVDGGLVHGDLKTSIDA

Sequences:

>Translated_267_residues
MNLTDMFGLDGRIALVTGGSRGIGKMIVEGYLAAGAARVYISARKTAQIEEAVADFETRYPGKVIGLPVDLSTVEGCRAL
ARELEAREERLDILVNNAGAAWGEPFEGFPEAGWDKVMDINVKSPFFLTQALHGLLKAAGTHERPAKVINIGSIDGMRLN
PWETYSYHASKAAILYLTKRLAARLVTDHILVTAIAPGAFQSDMNKAARDHGDAVAKSIPVKRIGVPEDMAGAAIYLASK
AGDYVVGDTITVDGGLVHGDLKTSIDA
>Mature_267_residues
MNLTDMFGLDGRIALVTGGSRGIGKMIVEGYLAAGAARVYISARKTAQIEEAVADFETRYPGKVIGLPVDLSTVEGCRAL
ARELEAREERLDILVNNAGAAWGEPFEGFPEAGWDKVMDINVKSPFFLTQALHGLLKAAGTHERPAKVINIGSIDGMRLN
PWETYSYHASKAAILYLTKRLAARLVTDHILVTAIAPGAFQSDMNKAARDHGDAVAKSIPVKRIGVPEDMAGAAIYLASK
AGDYVVGDTITVDGGLVHGDLKTSIDA

Specific function: Required for the synthesis of the beta-hydroxy acid moiety of rhamnolipids [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI19923817, Length=258, Percent_Identity=28.6821705426357, Blast_Score=109, Evalue=3e-24,
Organism=Homo sapiens, GI32483357, Length=259, Percent_Identity=32.8185328185328, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI109715829, Length=242, Percent_Identity=30.1652892561983, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI15277342, Length=255, Percent_Identity=30.5882352941176, Blast_Score=93, Evalue=3e-19,
Organism=Homo sapiens, GI5031737, Length=256, Percent_Identity=32.421875, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI126723750, Length=256, Percent_Identity=27.734375, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI40254992, Length=254, Percent_Identity=25.9842519685039, Blast_Score=75, Evalue=4e-14,
Organism=Homo sapiens, GI7705925, Length=257, Percent_Identity=27.6264591439689, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI33667109, Length=206, Percent_Identity=32.0388349514563, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI225579078, Length=248, Percent_Identity=29.4354838709677, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI66933014, Length=256, Percent_Identity=25.390625, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI10190704, Length=252, Percent_Identity=25.7936507936508, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI186928839, Length=208, Percent_Identity=31.7307692307692, Blast_Score=68, Evalue=7e-12,
Organism=Homo sapiens, GI31542939, Length=193, Percent_Identity=30.5699481865285, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI146231950, Length=214, Percent_Identity=31.7757009345794, Blast_Score=66, Evalue=3e-11,
Organism=Homo sapiens, GI126723191, Length=197, Percent_Identity=33.502538071066, Blast_Score=65, Evalue=6e-11,
Organism=Homo sapiens, GI10190746, Length=256, Percent_Identity=28.515625, Blast_Score=65, Evalue=7e-11,
Organism=Homo sapiens, GI166795268, Length=213, Percent_Identity=29.1079812206573, Blast_Score=65, Evalue=8e-11,
Organism=Escherichia coli, GI1789208, Length=256, Percent_Identity=35.546875, Blast_Score=128, Evalue=5e-31,
Organism=Escherichia coli, GI1790717, Length=260, Percent_Identity=33.0769230769231, Blast_Score=123, Evalue=1e-29,
Organism=Escherichia coli, GI1787335, Length=250, Percent_Identity=32, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI87082160, Length=261, Percent_Identity=31.4176245210728, Blast_Score=104, Evalue=5e-24,
Organism=Escherichia coli, GI1787905, Length=257, Percent_Identity=30.7392996108949, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1788459, Length=187, Percent_Identity=34.2245989304813, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI2367175, Length=253, Percent_Identity=30.4347826086957, Blast_Score=91, Evalue=7e-20,
Organism=Escherichia coli, GI87082100, Length=260, Percent_Identity=29.2307692307692, Blast_Score=88, Evalue=6e-19,
Organism=Escherichia coli, GI2367365, Length=258, Percent_Identity=28.2945736434109, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1789378, Length=254, Percent_Identity=31.1023622047244, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1786812, Length=262, Percent_Identity=23.6641221374046, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1789057, Length=260, Percent_Identity=25.3846153846154, Blast_Score=61, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI17555706, Length=250, Percent_Identity=31.6, Blast_Score=113, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17561402, Length=256, Percent_Identity=30.46875, Blast_Score=109, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI25147288, Length=250, Percent_Identity=31.2, Blast_Score=102, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17562906, Length=269, Percent_Identity=32.7137546468402, Blast_Score=101, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI17560676, Length=269, Percent_Identity=31.2267657992565, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17531453, Length=266, Percent_Identity=31.9548872180451, Blast_Score=95, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17562910, Length=263, Percent_Identity=28.8973384030418, Blast_Score=91, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI193204405, Length=265, Percent_Identity=29.4339622641509, Blast_Score=88, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17562908, Length=266, Percent_Identity=30.0751879699248, Blast_Score=87, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI115534694, Length=254, Percent_Identity=30.7086614173228, Blast_Score=81, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17551412, Length=273, Percent_Identity=27.4725274725275, Blast_Score=81, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17560332, Length=270, Percent_Identity=29.2592592592593, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17538480, Length=271, Percent_Identity=26.5682656826568, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17560220, Length=272, Percent_Identity=29.4117647058824, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI71994604, Length=253, Percent_Identity=27.6679841897233, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17565030, Length=268, Percent_Identity=30.9701492537313, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI71994600, Length=255, Percent_Identity=27.4509803921569, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17559104, Length=267, Percent_Identity=29.2134831460674, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17538486, Length=267, Percent_Identity=27.3408239700375, Blast_Score=74, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI17561272, Length=260, Percent_Identity=30, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17562990, Length=273, Percent_Identity=27.4725274725275, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17555612, Length=213, Percent_Identity=30.0469483568075, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17560150, Length=267, Percent_Identity=28.4644194756554, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI72000259, Length=277, Percent_Identity=25.9927797833935, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17562904, Length=269, Percent_Identity=26.7657992565056, Blast_Score=68, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17563726, Length=269, Percent_Identity=25.2788104089219, Blast_Score=64, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI17544670, Length=275, Percent_Identity=23.6363636363636, Blast_Score=64, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI17538182, Length=261, Percent_Identity=28.735632183908, Blast_Score=64, Evalue=7e-11,
Organism=Saccharomyces cerevisiae, GI6323882, Length=249, Percent_Identity=29.3172690763052, Blast_Score=84, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6324126, Length=260, Percent_Identity=25, Blast_Score=65, Evalue=1e-11,
Organism=Drosophila melanogaster, GI21355319, Length=257, Percent_Identity=32.6848249027237, Blast_Score=110, Evalue=8e-25,
Organism=Drosophila melanogaster, GI24643142, Length=253, Percent_Identity=35.5731225296443, Blast_Score=101, Evalue=6e-22,
Organism=Drosophila melanogaster, GI24639444, Length=250, Percent_Identity=28.8, Blast_Score=85, Evalue=6e-17,
Organism=Drosophila melanogaster, GI24644337, Length=185, Percent_Identity=31.3513513513514, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI28571526, Length=258, Percent_Identity=27.5193798449612, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI45553409, Length=209, Percent_Identity=30.1435406698565, Blast_Score=75, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24647948, Length=209, Percent_Identity=30.1435406698565, Blast_Score=75, Evalue=5e-14,
Organism=Drosophila melanogaster, GI21357041, Length=258, Percent_Identity=25.1937984496124, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI24647946, Length=209, Percent_Identity=30.1435406698565, Blast_Score=74, Evalue=8e-14,
Organism=Drosophila melanogaster, GI23397609, Length=256, Percent_Identity=26.5625, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24656154, Length=208, Percent_Identity=31.7307692307692, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI19922628, Length=208, Percent_Identity=31.7307692307692, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24644339, Length=258, Percent_Identity=26.3565891472868, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI17737361, Length=261, Percent_Identity=27.9693486590038, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI45554248, Length=211, Percent_Identity=29.3838862559242, Blast_Score=67, Evalue=9e-12,
Organism=Drosophila melanogaster, GI24640117, Length=211, Percent_Identity=29.3838862559242, Blast_Score=67, Evalue=9e-12,
Organism=Drosophila melanogaster, GI161076371, Length=200, Percent_Identity=32, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI19921754, Length=209, Percent_Identity=27.7511961722488, Blast_Score=65, Evalue=5e-11,
Organism=Drosophila melanogaster, GI21358495, Length=203, Percent_Identity=27.5862068965517, Blast_Score=64, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.1.1.100 [H]

Molecular weight: Translated: 28464; Mature: 28464

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLTDMFGLDGRIALVTGGSRGIGKMIVEGYLAAGAARVYISARKTAQIEEAVADFETRY
CCCCHHCCCCCEEEEEECCCCCHHHHHHHHHHHCCEEEEEEECCHHHHHHHHHHHHHHCC
PGKVIGLPVDLSTVEGCRALARELEAREERLDILVNNAGAAWGEPFEGFPEAGWDKVMDI
CCEEEEECCCHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCCCCCCCCCEEEE
NVKSPFFLTQALHGLLKAAGTHERPAKVINIGSIDGMRLNPWETYSYHASKAAILYLTKR
CCCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCEECCCCHHHHHHHHH
LAARLVTDHILVTAIAPGAFQSDMNKAARDHGDAVAKSIPVKRIGVPEDMAGAAIYLASK
HHHHHHHHHHEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEC
AGDYVVGDTITVDGGLVHGDLKTSIDA
CCCEEECCEEEECCCEEECCHHHCCCC
>Mature Secondary Structure
MNLTDMFGLDGRIALVTGGSRGIGKMIVEGYLAAGAARVYISARKTAQIEEAVADFETRY
CCCCHHCCCCCEEEEEECCCCCHHHHHHHHHHHCCEEEEEEECCHHHHHHHHHHHHHHCC
PGKVIGLPVDLSTVEGCRALARELEAREERLDILVNNAGAAWGEPFEGFPEAGWDKVMDI
CCEEEEECCCHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCCCCCCCCCEEEE
NVKSPFFLTQALHGLLKAAGTHERPAKVINIGSIDGMRLNPWETYSYHASKAAILYLTKR
CCCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCEECCCCHHHHHHHHH
LAARLVTDHILVTAIAPGAFQSDMNKAARDHGDAVAKSIPVKRIGVPEDMAGAAIYLASK
HHHHHHHHHHEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEC
AGDYVVGDTITVDGGLVHGDLKTSIDA
CCCEEECCEEEECCCEEECCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9721281; 10984043 [H]