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The map label for this gene is sseA [H]

Identifier: 103488537

GI number: 103488537

Start: 3212114

End: 3212953

Strand: Reverse

Name: sseA [H]

Synonym: Sala_3061

Alternate gene names: 103488537

Gene position: 3212953-3212114 (Counterclockwise)

Preceding gene: 103488538

Following gene: 103488536

Centisome position: 96.05

GC content: 68.45

Gene sequence:

>840_bases
ATGGACGCCTTGGTCTCAACCGACTGGCTGGAACGCGAACTGGGGGCATCGGACCTGCGGGTCGTCGATGCGACGAAATT
CCTGGGGACCGATCGCGACGCGCGCGCCGAATATGAGGCGGGCCACATCCCCGGCGCGGTGTTCATGGACCTCGCCGACC
TGACCGACACGTCCAGCGCGATCGAGAATATGGCGCCGCCCGCCGAAAAATTCGCCAGCCGGATGCAGTCGCTCGGCCTC
GGTGACGGCAGCCGCATCGTGCTTTACGACGACAGCCCGCTCAAGAGCGCGGCGCGCGCCTGGTGGCTGCTCAAATTGTT
CGGCGCGCATGACGTCGCGCTGCTCGACGGCGGGCTTGCCAAGTGGAAGGCCGAGGGCCGCGCGCTCGAAATGGGCAAGC
AGACGCTGCGCCACCGTCACTTCACCGTCTGGCGCGATCCCAAGGCGGTGCGCACCAAGGAACAGATGCTCGCCAACCTG
GACAGCGGCGCCGAACAGGTCGTCGATGCGCGCCCCGCGGCGCGTTTCACCGGTGAGGAACGCGATCCGCGCCCCGGCCT
CGCGCCCGGCCATATTCCCGGTTCGCGCAGCCTGCCGCACAGCGAATTGTTCAACGCCGACGGCACGTGGAAGCGCGGCG
ACGCGCTGAAAGCCGCTTTCGACGCCGCGGGAATCGACCTCGACAAACCACTCGTCGCAACCTGCGGCAGCGGCATGACC
GCCACCGTCGTCGCCTTTGGCGCCCACCTGCTCGGCAAGGACGATGTCGCGGTCTATGACGGCAGCTGGCTCGAATGGGG
CGCCGACGCCGCGACGCCCAAGGCGACCGGCGCAGCCTGA

Upstream 100 bases:

>100_bases
TGGGGGCATAGGCTGATGATCCCCCGAAAGAAGCCACAGCAGGATGGCCGGGCGAGGGACAGGCAGAAGCTTCCCCGACC
GGCGGTAACAGGAGCATGAC

Downstream 100 bases:

>100_bases
TCGCGGGCGCAGCTTTCGCCCCGCAATGCTTTTTCTGGCTTGTCTCACCCCGGCGCCGCGCTAATCTCGCGGCATCGGGG
CAAGGCAACCTCCCGGTCAG

Product: 3-mercaptopyruvate sulfurtransferase

Products: NA

Alternate protein names: MST; Rhodanese-like protein [H]

Number of amino acids: Translated: 279; Mature: 279

Protein sequence:

>279_residues
MDALVSTDWLERELGASDLRVVDATKFLGTDRDARAEYEAGHIPGAVFMDLADLTDTSSAIENMAPPAEKFASRMQSLGL
GDGSRIVLYDDSPLKSAARAWWLLKLFGAHDVALLDGGLAKWKAEGRALEMGKQTLRHRHFTVWRDPKAVRTKEQMLANL
DSGAEQVVDARPAARFTGEERDPRPGLAPGHIPGSRSLPHSELFNADGTWKRGDALKAAFDAAGIDLDKPLVATCGSGMT
ATVVAFGAHLLGKDDVAVYDGSWLEWGADAATPKATGAA

Sequences:

>Translated_279_residues
MDALVSTDWLERELGASDLRVVDATKFLGTDRDARAEYEAGHIPGAVFMDLADLTDTSSAIENMAPPAEKFASRMQSLGL
GDGSRIVLYDDSPLKSAARAWWLLKLFGAHDVALLDGGLAKWKAEGRALEMGKQTLRHRHFTVWRDPKAVRTKEQMLANL
DSGAEQVVDARPAARFTGEERDPRPGLAPGHIPGSRSLPHSELFNADGTWKRGDALKAAFDAAGIDLDKPLVATCGSGMT
ATVVAFGAHLLGKDDVAVYDGSWLEWGADAATPKATGAA
>Mature_279_residues
MDALVSTDWLERELGASDLRVVDATKFLGTDRDARAEYEAGHIPGAVFMDLADLTDTSSAIENMAPPAEKFASRMQSLGL
GDGSRIVLYDDSPLKSAARAWWLLKLFGAHDVALLDGGLAKWKAEGRALEMGKQTLRHRHFTVWRDPKAVRTKEQMLANL
DSGAEQVVDARPAARFTGEERDPRPGLAPGHIPGSRSLPHSELFNADGTWKRGDALKAAFDAAGIDLDKPLVATCGSGMT
ATVVAFGAHLLGKDDVAVYDGSWLEWGADAATPKATGAA

Specific function: Transfers a sulfur ion to cyanide or to other thiol compounds. Also has weak rhodanese activity. Its participation in detoxification of cyanide may be small. May be involved in the enhancement of serine sensitivity [H]

COG id: COG2897

COG function: function code P; Rhodanese-related sulfurtransferase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 rhodanese domains [H]

Homologues:

Organism=Homo sapiens, GI194473668, Length=271, Percent_Identity=43.9114391143911, Blast_Score=224, Evalue=7e-59,
Organism=Homo sapiens, GI61835204, Length=271, Percent_Identity=43.9114391143911, Blast_Score=224, Evalue=9e-59,
Organism=Homo sapiens, GI194473681, Length=271, Percent_Identity=43.9114391143911, Blast_Score=224, Evalue=9e-59,
Organism=Homo sapiens, GI17402865, Length=271, Percent_Identity=40.2214022140221, Blast_Score=193, Evalue=2e-49,
Organism=Escherichia coli, GI87082121, Length=272, Percent_Identity=40.8088235294118, Blast_Score=207, Evalue=5e-55,
Organism=Escherichia coli, GI87081967, Length=251, Percent_Identity=27.0916334661355, Blast_Score=77, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17561888, Length=292, Percent_Identity=26.7123287671233, Blast_Score=95, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI71997283, Length=297, Percent_Identity=25.9259259259259, Blast_Score=87, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI115534702, Length=247, Percent_Identity=25.9109311740891, Blast_Score=85, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17559150, Length=276, Percent_Identity=26.0869565217391, Blast_Score=78, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17543836, Length=277, Percent_Identity=23.4657039711191, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17543838, Length=277, Percent_Identity=23.4657039711191, Blast_Score=72, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6324825, Length=266, Percent_Identity=28.5714285714286, Blast_Score=105, Evalue=1e-23,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763
- InterPro:   IPR001307 [H]

Pfam domain/function: PF00581 Rhodanese [H]

EC number: =2.8.1.2 [H]

Molecular weight: Translated: 29880; Mature: 29880

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: PS00683 RHODANESE_2 ; PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDALVSTDWLERELGASDLRVVDATKFLGTDRDARAEYEAGHIPGAVFMDLADLTDTSSA
CCCCCCCHHHHHHCCCCCEEEEEHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHH
IENMAPPAEKFASRMQSLGLGDGSRIVLYDDSPLKSAARAWWLLKLFGAHDVALLDGGLA
HHHCCCCHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCEEECCCCHH
KWKAEGRALEMGKQTLRHRHFTVWRDPKAVRTKEQMLANLDSGAEQVVDARPAARFTGEE
HHHCCCHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHCCHHHHHHHCCCCHHCCCCC
RDPRPGLAPGHIPGSRSLPHSELFNADGTWKRGDALKAAFDAAGIDLDKPLVATCGSGMT
CCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCHHHHHHHHCCCCCCCCHHHHCCCCCH
ATVVAFGAHLLGKDDVAVYDGSWLEWGADAATPKATGAA
HHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MDALVSTDWLERELGASDLRVVDATKFLGTDRDARAEYEAGHIPGAVFMDLADLTDTSSA
CCCCCCCHHHHHHCCCCCEEEEEHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHH
IENMAPPAEKFASRMQSLGLGDGSRIVLYDDSPLKSAARAWWLLKLFGAHDVALLDGGLA
HHHCCCCHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCEEECCCCHH
KWKAEGRALEMGKQTLRHRHFTVWRDPKAVRTKEQMLANLDSGAEQVVDARPAARFTGEE
HHHCCCHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHCCHHHHHHHCCCCHHCCCCC
RDPRPGLAPGHIPGSRSLPHSELFNADGTWKRGDALKAAFDAAGIDLDKPLVATCGSGMT
CCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCHHHHHHHHCCCCCCCCHHHHCCCCCH
ATVVAFGAHLLGKDDVAVYDGSWLEWGADAATPKATGAA
HHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]