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The map label for this gene is kefA [C]

Identifier: 103488534

GI number: 103488534

Start: 3208086

End: 3209393

Strand: Reverse

Name: kefA [C]

Synonym: Sala_3058

Alternate gene names: 103488534

Gene position: 3209393-3208086 (Counterclockwise)

Preceding gene: 103488535

Following gene: 103488524

Centisome position: 95.94

GC content: 65.21

Gene sequence:

>1308_bases
ATGAACGAACTCGACGCGCTGTTCGCCCGCATGGGTTTTCCGGCGCTCGCCGAGGCGCAGCTGGTCGAGCTGGGCGTCGC
GGGGGGGCTGGCGGTGTTTGCGCTCGCCACGAGCTGGCTGGTCGGAAAACGGATCGGTCCCCGGCTTGCCGACTGGCTGC
ACGGGCAGGATCTGGGTGCGCGCGGACCGCTCACCGAACGCGCCGGCGCGATGCTCGGCTGGATCGCCGCGCTGCTCATC
GTCGGTCTCGCCGGTGCGCTCTGGCCGTGGCATCCCTATGCGCTGCTGCTGCTGAACCTGCTGTTCGCGCTCGGTGTTGC
GGCGCTGGCGCGGCACCTATTGATCGGCGTCGGGCTGGGCGACGGGGCCGCGCTGGCCGGGGCATTCGTGGCGCTCGTCG
TCATGATGTCGCGTGCGGTCGGCGGGCTGGAGTTGTTGCAGGAACGGCTCGACGCCTTCGGCTTTTCGATCGGCGACCGG
CATTTTTCGTTGCTCACCGTCGTCAACCTGCTGGTCACCGTGGTCATCCTTTTCACGCTGGTTCGGCTGGCGACGCGCGT
GCTGCGGCGCACGATCAGGGGTAGCGAGCGTTTCGATCCGACGCAGCGGCTGCTCATCGAAAAGATTTCGGCGGTGCTGA
TCGTCGTCGCCGCCTTCTTTGTCGGCATCGACATATTGGGCATCGACCTCACCGCCTTTGCGGTGTTTTCCGGCGCGCTC
GGTCTTGCAATCGGTTTCGGGCTGCAAAAGACCGTCGGCAATCTGATCGCGGGCATCATCCTGCTGATGGACCGCTCGAT
CAAGCCCGGCGACGTCATCGCGGTCGGCGACAGTTTCGGATGGGTGAACAAGATCGGCGTGCGCGCCGTGTCGGTGATCA
CGCGCGAGGGCAAGGAACATCTGATCCCCAACGAAATCCTGATGACGCAGGAGGTCGAGAACTGGAGCTATTCAAGCCGC
ACGGTGCGCGTGAGCATCGCGGTGGGGGTCGCCTATGACACCGACCTGCGGCTGGCGCAGCAGCTGATGATCGAGGCCGC
CAAGGAAGCGAAGCGTGTGCTCCCCGATCCTGCCCCGGTATGCTGGATCACCGGTTTCGGCGACAGCAGCGTCGACCATG
AGGTGCGCTTCTGGATCACCGACCCGGAGGCGGGGATCGGCAATATCAAGGGCGATGTGTTCCTGCGCATCTGGGACAAG
TTCCAGGCGGCGGGAATCGAAATCCCCTATCCGCAGCGCGATCTGCATGTGCGCGCGATGCCCGAAACGGCGACGAAGGC
AGCGAAGGCGACGAAGGCGAAAAGCTGA

Upstream 100 bases:

>100_bases
GCTGGACCGACCCCGACCCGCTCGTCCGCCTGTCGATCGGGCTCGAGGACCCGGCCGACCTGATCGCCGACCTCGAGCGC
GGCTTTGCCGCGATGACGGC

Downstream 100 bases:

>100_bases
ACCGTCGCCCTTCAGAACCCCACTTTCTTCGCCCGTTCGACGCATTCGACGATGATGCGCTTGGCCTCGTCGACATCGCC
CCAGCCGTTGAGCTTCGCCC

Product: MscS mechanosensitive ion channel

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 435; Mature: 435

Protein sequence:

>435_residues
MNELDALFARMGFPALAEAQLVELGVAGGLAVFALATSWLVGKRIGPRLADWLHGQDLGARGPLTERAGAMLGWIAALLI
VGLAGALWPWHPYALLLLNLLFALGVAALARHLLIGVGLGDGAALAGAFVALVVMMSRAVGGLELLQERLDAFGFSIGDR
HFSLLTVVNLLVTVVILFTLVRLATRVLRRTIRGSERFDPTQRLLIEKISAVLIVVAAFFVGIDILGIDLTAFAVFSGAL
GLAIGFGLQKTVGNLIAGIILLMDRSIKPGDVIAVGDSFGWVNKIGVRAVSVITREGKEHLIPNEILMTQEVENWSYSSR
TVRVSIAVGVAYDTDLRLAQQLMIEAAKEAKRVLPDPAPVCWITGFGDSSVDHEVRFWITDPEAGIGNIKGDVFLRIWDK
FQAAGIEIPYPQRDLHVRAMPETATKAAKATKAKS

Sequences:

>Translated_435_residues
MNELDALFARMGFPALAEAQLVELGVAGGLAVFALATSWLVGKRIGPRLADWLHGQDLGARGPLTERAGAMLGWIAALLI
VGLAGALWPWHPYALLLLNLLFALGVAALARHLLIGVGLGDGAALAGAFVALVVMMSRAVGGLELLQERLDAFGFSIGDR
HFSLLTVVNLLVTVVILFTLVRLATRVLRRTIRGSERFDPTQRLLIEKISAVLIVVAAFFVGIDILGIDLTAFAVFSGAL
GLAIGFGLQKTVGNLIAGIILLMDRSIKPGDVIAVGDSFGWVNKIGVRAVSVITREGKEHLIPNEILMTQEVENWSYSSR
TVRVSIAVGVAYDTDLRLAQQLMIEAAKEAKRVLPDPAPVCWITGFGDSSVDHEVRFWITDPEAGIGNIKGDVFLRIWDK
FQAAGIEIPYPQRDLHVRAMPETATKAAKATKAKS
>Mature_435_residues
MNELDALFARMGFPALAEAQLVELGVAGGLAVFALATSWLVGKRIGPRLADWLHGQDLGARGPLTERAGAMLGWIAALLI
VGLAGALWPWHPYALLLLNLLFALGVAALARHLLIGVGLGDGAALAGAFVALVVMMSRAVGGLELLQERLDAFGFSIGDR
HFSLLTVVNLLVTVVILFTLVRLATRVLRRTIRGSERFDPTQRLLIEKISAVLIVVAAFFVGIDILGIDLTAFAVFSGAL
GLAIGFGLQKTVGNLIAGIILLMDRSIKPGDVIAVGDSFGWVNKIGVRAVSVITREGKEHLIPNEILMTQEVENWSYSSR
TVRVSIAVGVAYDTDLRLAQQLMIEAAKEAKRVLPDPAPVCWITGFGDSSVDHEVRFWITDPEAGIGNIKGDVFLRIWDK
FQAAGIEIPYPQRDLHVRAMPETATKAAKATKAKS

Specific function: Unknown

COG id: COG3264

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1786670, Length=210, Percent_Identity=32.3809523809524, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI2367355, Length=238, Percent_Identity=27.3109243697479, Blast_Score=108, Evalue=9e-25,
Organism=Escherichia coli, GI1789291, Length=258, Percent_Identity=26.7441860465116, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1787591, Length=254, Percent_Identity=24.8031496062992, Blast_Score=70, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685
- InterPro:   IPR006686
- InterPro:   IPR011014 [H]

Pfam domain/function: PF00924 MS_channel [H]

EC number: NA

Molecular weight: Translated: 46734; Mature: 46734

Theoretical pI: Translated: 8.47; Mature: 8.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNELDALFARMGFPALAEAQLVELGVAGGLAVFALATSWLVGKRIGPRLADWLHGQDLGA
CCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCC
RGPLTERAGAMLGWIAALLIVGLAGALWPWHPYALLLLNLLFALGVAALARHLLIGVGLG
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
DGAALAGAFVALVVMMSRAVGGLELLQERLDAFGFSIGDRHFSLLTVVNLLVTVVILFTL
CCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
VRLATRVLRRTIRGSERFDPTQRLLIEKISAVLIVVAAFFVGIDILGIDLTAFAVFSGAL
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLAIGFGLQKTVGNLIAGIILLMDRSIKPGDVIAVGDSFGWVNKIGVRAVSVITREGKEH
HHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCC
LIPNEILMTQEVENWSYSSRTVRVSIAVGVAYDTDLRLAQQLMIEAAKEAKRVLPDPAPV
CCCCHHHHHHHHCCCCCCCCEEEEEEEEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCE
CWITGFGDSSVDHEVRFWITDPEAGIGNIKGDVFLRIWDKFQAAGIEIPYPQRDLHVRAM
EEEECCCCCCCCCEEEEEEECCCCCCCCCCCHHEEEEHHHHHHCCCCCCCCCCCCEEEEC
PETATKAAKATKAKS
CHHHHHHHHHHCCCC
>Mature Secondary Structure
MNELDALFARMGFPALAEAQLVELGVAGGLAVFALATSWLVGKRIGPRLADWLHGQDLGA
CCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCC
RGPLTERAGAMLGWIAALLIVGLAGALWPWHPYALLLLNLLFALGVAALARHLLIGVGLG
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
DGAALAGAFVALVVMMSRAVGGLELLQERLDAFGFSIGDRHFSLLTVVNLLVTVVILFTL
CCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
VRLATRVLRRTIRGSERFDPTQRLLIEKISAVLIVVAAFFVGIDILGIDLTAFAVFSGAL
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLAIGFGLQKTVGNLIAGIILLMDRSIKPGDVIAVGDSFGWVNKIGVRAVSVITREGKEH
HHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCC
LIPNEILMTQEVENWSYSSRTVRVSIAVGVAYDTDLRLAQQLMIEAAKEAKRVLPDPAPV
CCCCHHHHHHHHCCCCCCCCEEEEEEEEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCE
CWITGFGDSSVDHEVRFWITDPEAGIGNIKGDVFLRIWDKFQAAGIEIPYPQRDLHVRAM
EEEECCCCCCCCCEEEEEEECCCCCCCCCCCHHEEEEHHHHHHCCCCCCCCCCCCEEEEC
PETATKAAKATKAKS
CHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023 [H]