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The map label for this gene is fixJ [H]

Identifier: 103488437

GI number: 103488437

Start: 3102820

End: 3103569

Strand: Direct

Name: fixJ [H]

Synonym: Sala_2960

Alternate gene names: 103488437

Gene position: 3102820-3103569 (Clockwise)

Preceding gene: 103488436

Following gene: 103488440

Centisome position: 92.76

GC content: 72.8

Gene sequence:

>750_bases
TTGGCGCGATTCAAATGCGAAAAAGCGATCCGTTATGGCGCCATCGCCGATGCGTTCGCGCTGTCGCGCGCGGCCGGGGT
CGGGCAGCGTGGAGACGTGACCGGACCCTGCGGCCATATCCATCTGATCCTTCCCGACCCGGTCGAGCGCGCGGCCTGCT
TCCGTGTGCTGACGGCGGGGCCCGCGCGCGTCGTGCGCAGCTTTGCCAGCGCCGACGACTGGTTTGCGGCGGGCGATGAC
GACAGCTGCGCGGTGCTCCTGCTGCGCTGGGAGCAGCCGGGCGCGGTAAGCGGTGCCGCGCTGCTGGCGCATGCCGCCGC
GCGCGACGGCATCGCCGCCTTCGTCGCCGCCGAAAGGCTGAGCATCGCCGAATCGCGCGCGATCCTGTTGAGCGGCGCGC
GCGACCTGCTTCCCGCGCCGCTCGATCCCCGGCTCGTGCGCCGCACGATCGACGGTGCGCTCGCCGACCACGCGGCCTGG
CAAGTGGCGTGCGAGCGCCAGCGCGCGGCCGCGGCGCGGCTCGCCGCGCTGACCCCGCGCGAACGCGACATATTGGAGGG
TATCGCCGCCGGGCTCGGCAACAAGGCGATCGCGCAGCGGCTCGATCTCAGCCCGCGCACGGTCGAGGTTCACCGCGCGA
ACATCATGCGCCGCGCGGGCGCGGGCCATGTCGCCGAACTGCTGCACCTGCGTTTCGTCGCCGAGCAGGCGCGCGTCGCG
CCGGGCAATCGGGTCCGTTTCGGCGCATGA

Upstream 100 bases:

>100_bases
CGTCGCGGGGCAACCGCGCCGAACGCGGCCACACCCGCGCCGACTGATCCCACGGTCGGTTCCAGCGAAATGCGTCGGCG
CGCTGCCGACGCATTTCCTG

Downstream 100 bases:

>100_bases
TCGCGGCGCTGCTGCGGCCGGATCGTCGCGCGCGGCGCTATAAGGAGTATGAACGGCGACCCGTCCTTGTGTTGGTCCCC
ATGGGCGCTGCCGTTCATCC

Product: LuxR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 249; Mature: 248

Protein sequence:

>249_residues
MARFKCEKAIRYGAIADAFALSRAAGVGQRGDVTGPCGHIHLILPDPVERAACFRVLTAGPARVVRSFASADDWFAAGDD
DSCAVLLLRWEQPGAVSGAALLAHAAARDGIAAFVAAERLSIAESRAILLSGARDLLPAPLDPRLVRRTIDGALADHAAW
QVACERQRAAAARLAALTPRERDILEGIAAGLGNKAIAQRLDLSPRTVEVHRANIMRRAGAGHVAELLHLRFVAEQARVA
PGNRVRFGA

Sequences:

>Translated_249_residues
MARFKCEKAIRYGAIADAFALSRAAGVGQRGDVTGPCGHIHLILPDPVERAACFRVLTAGPARVVRSFASADDWFAAGDD
DSCAVLLLRWEQPGAVSGAALLAHAAARDGIAAFVAAERLSIAESRAILLSGARDLLPAPLDPRLVRRTIDGALADHAAW
QVACERQRAAAARLAALTPRERDILEGIAAGLGNKAIAQRLDLSPRTVEVHRANIMRRAGAGHVAELLHLRFVAEQARVA
PGNRVRFGA
>Mature_248_residues
ARFKCEKAIRYGAIADAFALSRAAGVGQRGDVTGPCGHIHLILPDPVERAACFRVLTAGPARVVRSFASADDWFAAGDDD
SCAVLLLRWEQPGAVSGAALLAHAAARDGIAAFVAAERLSIAESRAILLSGARDLLPAPLDPRLVRRTIDGALADHAAWQ
VACERQRAAAARLAALTPRERDILEGIAAGLGNKAIAQRLDLSPRTVEVHRANIMRRAGAGHVAELLHLRFVAEQARVAP
GNRVRFGA

Specific function: FixJ, when activated by fixL, induces the expression of both nifA, required for activation of classical nif and fix genes, and fixK, required for fixN activation [H]

COG id: COG4566

COG function: function code T; Response regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

None

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 26377; Mature: 26245

Theoretical pI: Translated: 10.11; Mature: 10.11

Prosite motif: PS00622 HTH_LUXR_1 ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARFKCEKAIRYGAIADAFALSRAAGVGQRGDVTGPCGHIHLILPDPVERAACFRVLTAG
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHCC
PARVVRSFASADDWFAAGDDDSCAVLLLRWEQPGAVSGAALLAHAAARDGIAAFVAAERL
HHHHHHHHHCCCCCEECCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHCCHHHHHHHHHH
SIAESRAILLSGARDLLPAPLDPRLVRRTIDGALADHAAWQVACERQRAAAARLAALTPR
HHHHHHHHEEECCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
ERDILEGIAAGLGNKAIAQRLDLSPRTVEVHRANIMRRAGAGHVAELLHLRFVAEQARVA
HHHHHHHHHHCCCCHHHHHHHCCCCCEEHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCC
PGNRVRFGA
CCCEEECCC
>Mature Secondary Structure 
ARFKCEKAIRYGAIADAFALSRAAGVGQRGDVTGPCGHIHLILPDPVERAACFRVLTAG
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHCC
PARVVRSFASADDWFAAGDDDSCAVLLLRWEQPGAVSGAALLAHAAARDGIAAFVAAERL
HHHHHHHHHCCCCCEECCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHCCHHHHHHHHHH
SIAESRAILLSGARDLLPAPLDPRLVRRTIDGALADHAAWQVACERQRAAAARLAALTPR
HHHHHHHHEEECCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
ERDILEGIAAGLGNKAIAQRLDLSPRTVEVHRANIMRRAGAGHVAELLHLRFVAEQARVA
HHHHHHHHHHCCCCHHHHHHHCCCCCEEHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCC
PGNRVRFGA
CCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2000090; 12597275 [H]