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The map label for this gene is rlmE

Identifier: 103488231

GI number: 103488231

Start: 2902996

End: 2903688

Strand: Direct

Name: rlmE

Synonym: Sala_2754

Alternate gene names: 103488231

Gene position: 2902996-2903688 (Clockwise)

Preceding gene: 103488230

Following gene: 103488232

Centisome position: 86.78

GC content: 66.23

Gene sequence:

>693_bases
ATGAGCGGCAGCGGCGGCAAGGGCGGCGGCCGCGGCGGTCTGCACGTGCGGGTCAAGACCGCGAAAAGGCGCAGCGTGTC
GTCGACGCGCTGGCTGCAGCGGCAACTCAACGACCCCTATGTCCGCCGCGCGCAGGCCGAAGGCTATCGTTCGCGCGCGG
CCTACAAGCTGATTGAGCTCGACGAGAAGTTCGGCCTGCTCAAAAAATCGCGCGCCGTTGTCGATCTGGGGATTGCGCCC
GGCGGCTGGTCGCAGGTCGTCCGCAAGGCGAACCCGCGCGCGCGCGTCGCCGGAATCGACCTCTTGCCGTGCGAACCGAT
CGAGGGCGTCGCGATCCTCGAAATGGATTTCATGGATGATGCGGCGCCCGATGCCCTGATCGCGGCGCTGGGCGGTGCGC
CCGATCTCGTCATTTCGGACATGGCGGCGAACACCGTCGGCCATCCGCAGACCGATCATCTGCGCACCATCGGCCTTGCC
GAAACCGCCGCCGATTTCGCGGTGCGAAACCTGCTGCCCGGTGGCGCCTTTGTCGCCAAGGTCTTCGCCGGCGGTGCCGA
CCGCGAACTGCTCACGCTGCTCAAGCGTCATTTCACGACGGTCAAACACGCCAAACCGCCCGCGAGCCGCAAGGGATCGC
CCGAACTTTATGTGATCGCACAGGGTTTCAAGGGACGCGGTGATGCGGCATAA

Upstream 100 bases:

>100_bases
CGCGCGTCGGCGTCGCCGATCGCGGAATCCGCGAGGGCATCCTGCGCACGCTGGCGATGCAGGGCCGCGACATTCCGGTC
ACGCGCCGCGAATATCGCAA

Downstream 100 bases:

>100_bases
CCCCCTGTAAACGGACAGTCGGTTCGGGCCGGGGAGACGGGATGATGATGAAATCCAGGCAATCGCTGGCGGCGGTGACG
CTTGCCGCGTTGCTGCTGAC

Product: ribosomal RNA methyltransferase RrmJ/FtsJ

Products: NA

Alternate protein names: 23S rRNA Um2552 methyltransferase; rRNA (uridine-2'-O-)-methyltransferase

Number of amino acids: Translated: 230; Mature: 229

Protein sequence:

>230_residues
MSGSGGKGGGRGGLHVRVKTAKRRSVSSTRWLQRQLNDPYVRRAQAEGYRSRAAYKLIELDEKFGLLKKSRAVVDLGIAP
GGWSQVVRKANPRARVAGIDLLPCEPIEGVAILEMDFMDDAAPDALIAALGGAPDLVISDMAANTVGHPQTDHLRTIGLA
ETAADFAVRNLLPGGAFVAKVFAGGADRELLTLLKRHFTTVKHAKPPASRKGSPELYVIAQGFKGRGDAA

Sequences:

>Translated_230_residues
MSGSGGKGGGRGGLHVRVKTAKRRSVSSTRWLQRQLNDPYVRRAQAEGYRSRAAYKLIELDEKFGLLKKSRAVVDLGIAP
GGWSQVVRKANPRARVAGIDLLPCEPIEGVAILEMDFMDDAAPDALIAALGGAPDLVISDMAANTVGHPQTDHLRTIGLA
ETAADFAVRNLLPGGAFVAKVFAGGADRELLTLLKRHFTTVKHAKPPASRKGSPELYVIAQGFKGRGDAA
>Mature_229_residues
SGSGGKGGGRGGLHVRVKTAKRRSVSSTRWLQRQLNDPYVRRAQAEGYRSRAAYKLIELDEKFGLLKKSRAVVDLGIAPG
GWSQVVRKANPRARVAGIDLLPCEPIEGVAILEMDFMDDAAPDALIAALGGAPDLVISDMAANTVGHPQTDHLRTIGLAE
TAADFAVRNLLPGGAFVAKVFAGGADRELLTLLKRHFTTVKHAKPPASRKGSPELYVIAQGFKGRGDAA

Specific function: Specifically methylates the uridine in position 2552 of 23S rRNA at the 2'-O position of the ribose in the fully assembled 50S ribosomal subunit

COG id: COG0293

COG function: function code J; 23S rRNA methylase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmE family

Homologues:

Organism=Homo sapiens, GI7019377, Length=227, Percent_Identity=38.7665198237885, Blast_Score=145, Evalue=3e-35,
Organism=Homo sapiens, GI194097365, Length=188, Percent_Identity=37.2340425531915, Blast_Score=118, Evalue=5e-27,
Organism=Homo sapiens, GI29029591, Length=190, Percent_Identity=35.2631578947368, Blast_Score=110, Evalue=1e-24,
Organism=Homo sapiens, GI29029589, Length=190, Percent_Identity=35.2631578947368, Blast_Score=110, Evalue=1e-24,
Organism=Homo sapiens, GI7110661, Length=190, Percent_Identity=35.2631578947368, Blast_Score=110, Evalue=1e-24,
Organism=Escherichia coli, GI1789569, Length=207, Percent_Identity=45.4106280193237, Blast_Score=163, Evalue=7e-42,
Organism=Caenorhabditis elegans, GI17553474, Length=201, Percent_Identity=39.8009950248756, Blast_Score=143, Evalue=7e-35,
Organism=Caenorhabditis elegans, GI71987550, Length=193, Percent_Identity=35.7512953367876, Blast_Score=109, Evalue=9e-25,
Organism=Caenorhabditis elegans, GI71987561, Length=193, Percent_Identity=35.7512953367876, Blast_Score=108, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17554650, Length=195, Percent_Identity=34.8717948717949, Blast_Score=108, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17553860, Length=193, Percent_Identity=35.7512953367876, Blast_Score=108, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6319796, Length=191, Percent_Identity=34.0314136125654, Blast_Score=110, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6321302, Length=111, Percent_Identity=44.1441441441441, Blast_Score=106, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6319535, Length=199, Percent_Identity=32.1608040201005, Blast_Score=95, Evalue=7e-21,
Organism=Drosophila melanogaster, GI21356387, Length=222, Percent_Identity=37.3873873873874, Blast_Score=128, Evalue=3e-30,
Organism=Drosophila melanogaster, GI18859957, Length=205, Percent_Identity=37.5609756097561, Blast_Score=117, Evalue=7e-27,
Organism=Drosophila melanogaster, GI24648639, Length=196, Percent_Identity=35.2040816326531, Blast_Score=109, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24647580, Length=198, Percent_Identity=33.3333333333333, Blast_Score=108, Evalue=3e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLME_SPHAL (Q1GPG3)

Other databases:

- EMBL:   CP000356
- RefSeq:   YP_617792.1
- ProteinModelPortal:   Q1GPG3
- SMR:   Q1GPG3
- GeneID:   4080239
- GenomeReviews:   CP000356_GR
- KEGG:   sal:Sala_2754
- NMPDR:   fig|317655.9.peg.2619
- HOGENOM:   HBG398270
- OMA:   SDMAANT
- ProtClustDB:   CLSK835143
- BioCyc:   SALA317655:SALA_2754-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01547
- InterPro:   IPR015507
- InterPro:   IPR002877
- PANTHER:   PTHR10920
- PIRSF:   PIRSF005461

Pfam domain/function: PF01728 FtsJ

EC number: =2.1.1.166

Molecular weight: Translated: 24458; Mature: 24327

Theoretical pI: Translated: 10.63; Mature: 10.63

Prosite motif: NA

Important sites: ACT_SITE 180-180 BINDING 81-81 BINDING 83-83 BINDING 100-100 BINDING 116-116 BINDING 140-140

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGSGGKGGGRGGLHVRVKTAKRRSVSSTRWLQRQLNDPYVRRAQAEGYRSRAAYKLIEL
CCCCCCCCCCCCCEEEEEECCHHHCCHHHHHHHHHCCCHHHHHHHHHCHHHHHHHHHHHH
DEKFGLLKKSRAVVDLGIAPGGWSQVVRKANPRARVAGIDLLPCEPIEGVAILEMDFMDD
HHHHHHHHHCCEEEEECCCCCCHHHHHHCCCCCCEEECEECCCCCCCCCEEEEEECCCCC
AAPDALIAALGGAPDLVISDMAANTVGHPQTDHLRTIGLAETAADFAVRNLLPGGAFVAK
CCCHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHHCCCCHHHHHH
VFAGGADRELLTLLKRHFTTVKHAKPPASRKGSPELYVIAQGFKGRGDAA
HHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCCC
>Mature Secondary Structure 
SGSGGKGGGRGGLHVRVKTAKRRSVSSTRWLQRQLNDPYVRRAQAEGYRSRAAYKLIEL
CCCCCCCCCCCCEEEEEECCHHHCCHHHHHHHHHCCCHHHHHHHHHCHHHHHHHHHHHH
DEKFGLLKKSRAVVDLGIAPGGWSQVVRKANPRARVAGIDLLPCEPIEGVAILEMDFMDD
HHHHHHHHHCCEEEEECCCCCCHHHHHHCCCCCCEEECEECCCCCCCCCEEEEEECCCCC
AAPDALIAALGGAPDLVISDMAANTVGHPQTDHLRTIGLAETAADFAVRNLLPGGAFVAK
CCCHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHHCCCCHHHHHH
VFAGGADRELLTLLKRHFTTVKHAKPPASRKGSPELYVIAQGFKGRGDAA
HHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA