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The map label for this gene is fixJ [H]

Identifier: 103487939

GI number: 103487939

Start: 2602385

End: 2603017

Strand: Direct

Name: fixJ [H]

Synonym: Sala_2460

Alternate gene names: 103487939

Gene position: 2602385-2603017 (Clockwise)

Preceding gene: 103487938

Following gene: 103487940

Centisome position: 77.8

GC content: 60.19

Gene sequence:

>633_bases
ATGCAGGATAAGCGCCTCATTCATCTGGTCGATGACGAAGAGGCCATCCGGAAGTCAGCCGGATTCATGCTGCGGACGGT
GGGGTTCGAGGTACGGACCTATGCGAGCGGCGTGGAATTTCTGAAGCTGGCACGCACGGCCGAGACTGGATGTGTGCTTC
TCGACGTTCGCATGCCTGAGTTAGATGGGCTTCAGGTCCAGTCGGAAATGGCGCAGCGCGGCATCAATATGCCGGTCGTC
GTCCTGACCGGTCATGGCGACGTCACTCTCGCGGTGCAGGCGATGAAGGCGGGCGCAGTGGATTTTATCGAGAAACCCTT
CGAGAAGGCATCGCTGCTTGAGGCTATCGACCGCGGATTCGCGCGTATCGACCGGGTCGATGTCCGAGCGACGGAAGAAG
CCGAAGCGCAGGTCCGGATCGGCGTCTTGACGCCCCGCGAGAAGGAGGTTCTCGATGGGCTCGCGCGAGGCTATCCCAAC
AAGACCATCGCCTATGATCTAGGCGTTTCTTCGCGAACCGTCGAAGTCCACCGGGCAAGCCTGATGAGCAAGTTGGCTGT
CCGCAGTCTTTCCGATGCGCTCCGGATTGCATTTGCTGCGGGCCTTGGTCGCAGCGAAGCCAAACTGGGATGA

Upstream 100 bases:

>100_bases
ATCGTTGAGGCTCATGGCGGACGGATCTGGGTCGAGGACGGTCCAGGCGGTGGAGCCGTGTTCCGATTCACCTTGGTTCA
CGCAAAGCTGGAGGAAGAAC

Downstream 100 bases:

>100_bases
TTACGTAGCGACTGAGATACGCGGTTCTGGAATGCTGCACTCACGGAGAATCGATTTTCGGATGCTCGCCACTCATGTCT
GTGCACATCGGGCAATCGTC

Product: two component LuxR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 210; Mature: 210

Protein sequence:

>210_residues
MQDKRLIHLVDDEEAIRKSAGFMLRTVGFEVRTYASGVEFLKLARTAETGCVLLDVRMPELDGLQVQSEMAQRGINMPVV
VLTGHGDVTLAVQAMKAGAVDFIEKPFEKASLLEAIDRGFARIDRVDVRATEEAEAQVRIGVLTPREKEVLDGLARGYPN
KTIAYDLGVSSRTVEVHRASLMSKLAVRSLSDALRIAFAAGLGRSEAKLG

Sequences:

>Translated_210_residues
MQDKRLIHLVDDEEAIRKSAGFMLRTVGFEVRTYASGVEFLKLARTAETGCVLLDVRMPELDGLQVQSEMAQRGINMPVV
VLTGHGDVTLAVQAMKAGAVDFIEKPFEKASLLEAIDRGFARIDRVDVRATEEAEAQVRIGVLTPREKEVLDGLARGYPN
KTIAYDLGVSSRTVEVHRASLMSKLAVRSLSDALRIAFAAGLGRSEAKLG
>Mature_210_residues
MQDKRLIHLVDDEEAIRKSAGFMLRTVGFEVRTYASGVEFLKLARTAETGCVLLDVRMPELDGLQVQSEMAQRGINMPVV
VLTGHGDVTLAVQAMKAGAVDFIEKPFEKASLLEAIDRGFARIDRVDVRATEEAEAQVRIGVLTPREKEVLDGLARGYPN
KTIAYDLGVSSRTVEVHRASLMSKLAVRSLSDALRIAFAAGLGRSEAKLG

Specific function: FixJ, when activated by fixL, induces the expression of both nifA, required for activation of classical nif and fix genes, and fixK, required for fixN activation [H]

COG id: COG4566

COG function: function code T; Response regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=104, Percent_Identity=37.5, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1790299, Length=120, Percent_Identity=32.5, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI1788905, Length=109, Percent_Identity=38.5321100917431, Blast_Score=74, Evalue=7e-15,
Organism=Escherichia coli, GI1790102, Length=192, Percent_Identity=31.25, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1788222, Length=208, Percent_Identity=26.4423076923077, Blast_Score=68, Evalue=5e-13,
Organism=Escherichia coli, GI1790437, Length=160, Percent_Identity=27.5, Blast_Score=66, Evalue=1e-12,
Organism=Escherichia coli, GI1788521, Length=192, Percent_Identity=30.7291666666667, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1789402, Length=225, Percent_Identity=26.6666666666667, Blast_Score=60, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 22912; Mature: 22912

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00622 HTH_LUXR_1 ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQDKRLIHLVDDEEAIRKSAGFMLRTVGFEVRTYASGVEFLKLARTAETGCVLLDVRMPE
CCCCEEEEEECCHHHHHHHHCCEEEHHCCHHHHHHHHHHHHHHHHCCCCCEEEEEEECCC
LDGLQVQSEMAQRGINMPVVVLTGHGDVTLAVQAMKAGAVDFIEKPFEKASLLEAIDRGF
CCCCHHHHHHHHCCCCCCEEEEECCCCEEEEEEEHHCCCHHHHHCCHHHHHHHHHHHHHH
ARIDRVDVRATEEAEAQVRIGVLTPREKEVLDGLARGYPNKTIAYDLGVSSRTVEVHRAS
HHHHHCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHH
LMSKLAVRSLSDALRIAFAAGLGRSEAKLG
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MQDKRLIHLVDDEEAIRKSAGFMLRTVGFEVRTYASGVEFLKLARTAETGCVLLDVRMPE
CCCCEEEEEECCHHHHHHHHCCEEEHHCCHHHHHHHHHHHHHHHHCCCCCEEEEEEECCC
LDGLQVQSEMAQRGINMPVVVLTGHGDVTLAVQAMKAGAVDFIEKPFEKASLLEAIDRGF
CCCCHHHHHHHHCCCCCCEEEEECCCCEEEEEEEHHCCCHHHHHCCHHHHHHHHHHHHHH
ARIDRVDVRATEEAEAQVRIGVLTPREKEVLDGLARGYPNKTIAYDLGVSSRTVEVHRAS
HHHHHCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHH
LMSKLAVRSLSDALRIAFAAGLGRSEAKLG
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2842062; 11481432; 2663474; 1857213; 10647182 [H]