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The map label for this gene is fixL [H]

Identifier: 103487938

GI number: 103487938

Start: 2600851

End: 2602395

Strand: Direct

Name: fixL [H]

Synonym: Sala_2459

Alternate gene names: 103487938

Gene position: 2600851-2602395 (Clockwise)

Preceding gene: 103487937

Following gene: 103487939

Centisome position: 77.75

GC content: 61.62

Gene sequence:

>1545_bases
ATGATCCCCGAAGACAATTCGGTAGCGTGGCGCGACGACGTCCGCGAAATCGAGGCGCTATCGGAGCTCATGCTTCGCGA
GGCCAGCGGCTATGTCGTCGTCTTGCTCGATCCCGCCGGCACCATCCGCGCGATCAATGGCGAGACCGAGAGGAAGGGAC
TTTGGTCGAGCGCCGATCTGTGCGGCCGCTCGCACGAGATTTTCTATCCGCCCGATGAAATGGCGAACGGCCGGCCTGAA
GCCGATCTGGCGGCGGCCCTCGCGCAGGGTTCCCTCGAGCGTGAGGCTTGGCGCGTGTGCCGTGATGGAACCGAATATCT
CGCCAGGCTGACGATCAATGCCCTGAAAGATCAAGCGGGTGTGATTCGCGGTTTCGGCAGCATCGTGCGCGACATCACCG
AGGATGCGGCAATGCGCGGCTCGATCGAAGCCCGCGAGCGACATCTGCAGTCGATTCTCGCGACCGTTCCCGATGCCATG
ATCGTCATCGACGAGCGGGGGCGCATAACCTCGTTCAGCGCTGCGGCCGAGCGATTGTTCGGTTATGGCGAGAGCGAATT
GCTTGGCCGCAATGTGTCCTGCCTCATGCCGCGGGCGGACAGTATCAGGCATGACGAATATATCGGCCATTATCTCGAAA
CGGGAGAGCGCCGAATCATCGGGATCGGCCGCATCGTGGTCGGGCAGCGCCGGGACGGTTCGACTTTTCCAATGGAATTG
TCGGTCGGCGAGGCGGGGGGGCCGGAAGGGCGCATCTTCACCGGCTTCATTCGGGACCTGACGGAAAAGGAACATGATGA
GCTGAGGCTCAAGGAATTGCAGGCCGAGCTTGTGCATGTCTCGCGGCTGAGCGCGATGGGAACGATGGCCTCGACCTTGG
CCCATGAGCTTAACCAGCCGCTGACTGCCATTGCCAACTATCTGGAGACGACGCGGGATCTTGTTGACGGGGAAGGGGAT
ATACCGCGGGATATCCTGCACGAAGCGCTGACGGAGGCCGCCAATGAGACCCTCAGGGCAGGCTATATTGTTCGCCGCCT
CAGGGATTTCGTAGCTCGGGGCGAGGTGGAAAAGACCGTAGAGGATTTGCCGCGCCTGATCGACGAGGCAGGCAAGCTGG
CGCTTATCGGTGCCCGGGAACGCGGGGTCCGCAGCTTTTTCACCTTCGACCCGGCCGCGACACCTACGCTCGTGGACCGC
GTCCAGATCCAGCAGGTTCTGGTCAACATCATGCGCAATGCCATTGAAGCGATGGCGGACTCCGAAGTGCGGGAACTGCG
CGTTTCCACCAGGCTGCGGGCCGACGAGATGATCGAGATTGCGGTAGAGGACAGCGGTCCCGGCATTGCGGAGGACGTGA
TACCGCGGTTGTTCCAGGCTTTTGTCAGCAGCAAATCGGACGGCATGGGGCTCGGCCTGTCGATCTGTCGGACGATCGTT
GAGGCTCATGGCGGACGGATCTGGGTCGAGGACGGTCCAGGCGGTGGAGCCGTGTTCCGATTCACCTTGGTTCACGCAAA
GCTGGAGGAAGAACATGCAGGATAA

Upstream 100 bases:

>100_bases
TGCGTATTTGACTTAGTGCCCGTTCCGATTGGGGGATTCCGACGGCCATGAAACCATTGCCCTGTCGCCTACGCGCCCGG
CCTGGACGGCTGGGGATGAC

Downstream 100 bases:

>100_bases
GCGCCTCATTCATCTGGTCGATGACGAAGAGGCCATCCGGAAGTCAGCCGGATTCATGCTGCGGACGGTGGGGTTCGAGG
TACGGACCTATGCGAGCGGC

Product: PAS/PAC sensor signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 514; Mature: 514

Protein sequence:

>514_residues
MIPEDNSVAWRDDVREIEALSELMLREASGYVVVLLDPAGTIRAINGETERKGLWSSADLCGRSHEIFYPPDEMANGRPE
ADLAAALAQGSLEREAWRVCRDGTEYLARLTINALKDQAGVIRGFGSIVRDITEDAAMRGSIEARERHLQSILATVPDAM
IVIDERGRITSFSAAAERLFGYGESELLGRNVSCLMPRADSIRHDEYIGHYLETGERRIIGIGRIVVGQRRDGSTFPMEL
SVGEAGGPEGRIFTGFIRDLTEKEHDELRLKELQAELVHVSRLSAMGTMASTLAHELNQPLTAIANYLETTRDLVDGEGD
IPRDILHEALTEAANETLRAGYIVRRLRDFVARGEVEKTVEDLPRLIDEAGKLALIGARERGVRSFFTFDPAATPTLVDR
VQIQQVLVNIMRNAIEAMADSEVRELRVSTRLRADEMIEIAVEDSGPGIAEDVIPRLFQAFVSSKSDGMGLGLSICRTIV
EAHGGRIWVEDGPGGGAVFRFTLVHAKLEEEHAG

Sequences:

>Translated_514_residues
MIPEDNSVAWRDDVREIEALSELMLREASGYVVVLLDPAGTIRAINGETERKGLWSSADLCGRSHEIFYPPDEMANGRPE
ADLAAALAQGSLEREAWRVCRDGTEYLARLTINALKDQAGVIRGFGSIVRDITEDAAMRGSIEARERHLQSILATVPDAM
IVIDERGRITSFSAAAERLFGYGESELLGRNVSCLMPRADSIRHDEYIGHYLETGERRIIGIGRIVVGQRRDGSTFPMEL
SVGEAGGPEGRIFTGFIRDLTEKEHDELRLKELQAELVHVSRLSAMGTMASTLAHELNQPLTAIANYLETTRDLVDGEGD
IPRDILHEALTEAANETLRAGYIVRRLRDFVARGEVEKTVEDLPRLIDEAGKLALIGARERGVRSFFTFDPAATPTLVDR
VQIQQVLVNIMRNAIEAMADSEVRELRVSTRLRADEMIEIAVEDSGPGIAEDVIPRLFQAFVSSKSDGMGLGLSICRTIV
EAHGGRIWVEDGPGGGAVFRFTLVHAKLEEEHAG
>Mature_514_residues
MIPEDNSVAWRDDVREIEALSELMLREASGYVVVLLDPAGTIRAINGETERKGLWSSADLCGRSHEIFYPPDEMANGRPE
ADLAAALAQGSLEREAWRVCRDGTEYLARLTINALKDQAGVIRGFGSIVRDITEDAAMRGSIEARERHLQSILATVPDAM
IVIDERGRITSFSAAAERLFGYGESELLGRNVSCLMPRADSIRHDEYIGHYLETGERRIIGIGRIVVGQRRDGSTFPMEL
SVGEAGGPEGRIFTGFIRDLTEKEHDELRLKELQAELVHVSRLSAMGTMASTLAHELNQPLTAIANYLETTRDLVDGEGD
IPRDILHEALTEAANETLRAGYIVRRLRDFVARGEVEKTVEDLPRLIDEAGKLALIGARERGVRSFFTFDPAATPTLVDR
VQIQQVLVNIMRNAIEAMADSEVRELRVSTRLRADEMIEIAVEDSGPGIAEDVIPRLFQAFVSSKSDGMGLGLSICRTIV
EAHGGRIWVEDGPGGGAVFRFTLVHAKLEEEHAG

Specific function: Putative oxygen sensor; modulates the activity of fixJ, a transcriptional activator of nitrogen fixation fixK gene. FixL probably acts as a kinase that phosphorylates fixJ [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PAS (PER-ARNT-SIM) domains [H]

Homologues:

Organism=Escherichia coli, GI1788549, Length=357, Percent_Identity=28.0112044817927, Blast_Score=119, Evalue=6e-28,
Organism=Escherichia coli, GI1790436, Length=234, Percent_Identity=31.1965811965812, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI48994928, Length=416, Percent_Identity=25, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1788393, Length=214, Percent_Identity=27.5700934579439, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1790300, Length=122, Percent_Identity=37.7049180327869, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1786600, Length=364, Percent_Identity=24.4505494505494, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1788713, Length=409, Percent_Identity=20.7823960880196, Blast_Score=69, Evalue=7e-13,
Organism=Escherichia coli, GI87081921, Length=123, Percent_Identity=28.4552845528455, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1786912, Length=122, Percent_Identity=35.2459016393443, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI145693157, Length=258, Percent_Identity=29.4573643410853, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1789149, Length=224, Percent_Identity=26.3392857142857, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR001610
- InterPro:   IPR000014
- InterPro:   IPR000700
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 56623; Mature: 56623

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: PS50112 PAS ; PS50113 PAC ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPEDNSVAWRDDVREIEALSELMLREASGYVVVLLDPAGTIRAINGETERKGLWSSADL
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCEEEECCCHHHCCCCCCHHH
CGRSHEIFYPPDEMANGRPEADLAAALAQGSLEREAWRVCRDGTEYLARLTINALKDQAG
CCCCCCEECCCHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VIRGFGSIVRDITEDAAMRGSIEARERHLQSILATVPDAMIVIDERGRITSFSAAAERLF
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHH
GYGESELLGRNVSCLMPRADSIRHDEYIGHYLETGERRIIGIGRIVVGQRRDGSTFPMEL
CCCCHHHHCCCCCEECCCCCCCCHHHHHHHHHHCCCEEEEEEEEEEEECCCCCCCCCEEE
SVGEAGGPEGRIFTGFIRDLTEKEHDELRLKELQAELVHVSRLSAMGTMASTLAHELNQP
ECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
LTAIANYLETTRDLVDGEGDIPRDILHEALTEAANETLRAGYIVRRLRDFVARGEVEKTV
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHH
EDLPRLIDEAGKLALIGARERGVRSFFTFDPAATPTLVDRVQIQQVLVNIMRNAIEAMAD
HHHHHHHHHCCCEEEEECHHHCCHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
SEVRELRVSTRLRADEMIEIAVEDSGPGIAEDVIPRLFQAFVSSKSDGMGLGLSICRTIV
HHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
EAHGGRIWVEDGPGGGAVFRFTLVHAKLEEEHAG
HHCCCEEEEEECCCCCCEEEHHHHHHHHHHCCCC
>Mature Secondary Structure
MIPEDNSVAWRDDVREIEALSELMLREASGYVVVLLDPAGTIRAINGETERKGLWSSADL
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCEEEECCCHHHCCCCCCHHH
CGRSHEIFYPPDEMANGRPEADLAAALAQGSLEREAWRVCRDGTEYLARLTINALKDQAG
CCCCCCEECCCHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VIRGFGSIVRDITEDAAMRGSIEARERHLQSILATVPDAMIVIDERGRITSFSAAAERLF
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHH
GYGESELLGRNVSCLMPRADSIRHDEYIGHYLETGERRIIGIGRIVVGQRRDGSTFPMEL
CCCCHHHHCCCCCEECCCCCCCCHHHHHHHHHHCCCEEEEEEEEEEEECCCCCCCCCEEE
SVGEAGGPEGRIFTGFIRDLTEKEHDELRLKELQAELVHVSRLSAMGTMASTLAHELNQP
ECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
LTAIANYLETTRDLVDGEGDIPRDILHEALTEAANETLRAGYIVRRLRDFVARGEVEKTV
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHH
EDLPRLIDEAGKLALIGARERGVRSFFTFDPAATPTLVDRVQIQQVLVNIMRNAIEAMAD
HHHHHHHHHCCCEEEEECHHHCCHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
SEVRELRVSTRLRADEMIEIAVEDSGPGIAEDVIPRLFQAFVSSKSDGMGLGLSICRTIV
HHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
EAHGGRIWVEDGPGGGAVFRFTLVHAKLEEEHAG
HHCCCEEEEEECCCCCCEEEHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2000090; 12597275; 9860942; 10747783 [H]