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The map label for this gene is cnrH [H]

Identifier: 103487903

GI number: 103487903

Start: 2561269

End: 2561853

Strand: Reverse

Name: cnrH [H]

Synonym: Sala_2423

Alternate gene names: 103487903

Gene position: 2561853-2561269 (Counterclockwise)

Preceding gene: 103487904

Following gene: 103487902

Centisome position: 76.58

GC content: 63.25

Gene sequence:

>585_bases
GTGAGCGCCGACCTATCGCAATGTAGCGACCAGGATCTGGCGGCCTTTGCGCGCGCGCACCGCGAAGACGCCTATCGCGA
GTTGCTGCGCCGTTACAAAACGGGCGTCTACCGGCTCATCGTAAAGCAGATCGGCGATGCCGACGAGGCGATGGATTTGA
CGCAGGAGACGTTCGTCTCCGGCTTTTCGGCGCTCGATCGCTATGATGGCGAACGCCCCTTCCGCACATGGATCGCCCGT
ATCGCGCTCAACAAATGTCGCGACTGGGCACGACGGCGAAAGGTGCGCGCCTTTTTCTCCCGCGCGCTCCCGCTCGAAAA
TGCGCATCACGTAGCAAGCGATGGTCCTGGGCCCGATTCCGCGGCGACCGACAGGCGAGAGCTCGCCAGGGTTCGCGGGG
CGATCGATCAGCTGCCGCAGAATTTGCGCGAAGTGTTGCTCCTCCGCGGTGTCGACGAGGTCAGCCAGGCCGAAACAGCG
GTCATTTTGCGGGTCAGCGAGAAAACAGTCGAAACGCGCCTCTATCGAGCGCGGACCAGGCTCCGGGAATTGCTCGCGGG
AACATCTGCGAGCGCCGAGGGTTGA

Upstream 100 bases:

>100_bases
AAACGCTCCAGCATCTCTTCGCGATGCGCGGGGTTCTCAACCCCGATCAAGCGGAAATGTTCGACAAAAGCGTGGTCAAG
GCGCTGACCGCCGATGCGCG

Downstream 100 bases:

>100_bases
GGGTTCGACCGCTCGTTCAGGCGGCATCTCCTCATCGTCCGGCATTTTTATTGAGGGGCAGGCGTGCCGCCTGCGTATAG
CTTTAGGAACAGTCAGTAAC

Product: ECF subfamily RNA polymerase sigma-24 factor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 194; Mature: 193

Protein sequence:

>194_residues
MSADLSQCSDQDLAAFARAHREDAYRELLRRYKTGVYRLIVKQIGDADEAMDLTQETFVSGFSALDRYDGERPFRTWIAR
IALNKCRDWARRRKVRAFFSRALPLENAHHVASDGPGPDSAATDRRELARVRGAIDQLPQNLREVLLLRGVDEVSQAETA
VILRVSEKTVETRLYRARTRLRELLAGTSASAEG

Sequences:

>Translated_194_residues
MSADLSQCSDQDLAAFARAHREDAYRELLRRYKTGVYRLIVKQIGDADEAMDLTQETFVSGFSALDRYDGERPFRTWIAR
IALNKCRDWARRRKVRAFFSRALPLENAHHVASDGPGPDSAATDRRELARVRGAIDQLPQNLREVLLLRGVDEVSQAETA
VILRVSEKTVETRLYRARTRLRELLAGTSASAEG
>Mature_193_residues
SADLSQCSDQDLAAFARAHREDAYRELLRRYKTGVYRLIVKQIGDADEAMDLTQETFVSGFSALDRYDGERPFRTWIARI
ALNKCRDWARRRKVRAFFSRALPLENAHHVASDGPGPDSAATDRRELARVRGAIDQLPQNLREVLLLRGVDEVSQAETAV
ILRVSEKTVETRLYRARTRLRELLAGTSASAEG

Specific function: CnrH alone is able to activate CNR expression, while both CnrY and CrnX are needed for nickel induction of cnrH (PubMed:10671463). Binds DNA in a RNA polymerase-dependent fashion (PubMed:10671464). CnrH may be controlled by a CnrYX transmembrane anti-sigm

COG id: COG1595

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788926, Length=176, Percent_Identity=28.9772727272727, Blast_Score=72, Evalue=3e-14,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000838
- InterPro:   IPR007627
- InterPro:   IPR013249
- InterPro:   IPR013325
- InterPro:   IPR013324 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF08281 Sigma70_r4_2 [H]

EC number: NA

Molecular weight: Translated: 21962; Mature: 21831

Theoretical pI: Translated: 9.51; Mature: 9.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSADLSQCSDQDLAAFARAHREDAYRELLRRYKTGVYRLIVKQIGDADEAMDLTQETFVS
CCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
GFSALDRYDGERPFRTWIARIALNKCRDWARRRKVRAFFSRALPLENAHHVASDGPGPDS
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCC
AATDRRELARVRGAIDQLPQNLREVLLLRGVDEVSQAETAVILRVSEKTVETRLYRARTR
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRELLAGTSASAEG
HHHHHHCCCCCCCC
>Mature Secondary Structure 
SADLSQCSDQDLAAFARAHREDAYRELLRRYKTGVYRLIVKQIGDADEAMDLTQETFVS
CCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
GFSALDRYDGERPFRTWIARIALNKCRDWARRRKVRAFFSRALPLENAHHVASDGPGPDS
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCC
AATDRRELARVRGAIDQLPQNLREVLLLRGVDEVSQAETAVILRVSEKTVETRLYRARTR
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRELLAGTSASAEG
HHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8380802; 8052622; 10671463; 10671464 [H]