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The map label for this gene is folA [H]

Identifier: 103487841

GI number: 103487841

Start: 2486323

End: 2486820

Strand: Reverse

Name: folA [H]

Synonym: Sala_2360

Alternate gene names: 103487841

Gene position: 2486820-2486323 (Counterclockwise)

Preceding gene: 103487842

Following gene: 103487840

Centisome position: 74.34

GC content: 68.47

Gene sequence:

>498_bases
ATGAACCACCCCGAAATCACGCTGATCCTGGCGCGCGCGGCCAATGGCGTGATCGGCGCGAAGGGCCGGATGCCGTGGCA
CCTGCCCGCTGACCTCCGCCGGTTCAAACAGCTCACCATGGGCCGCCCGATGATCATGGGGCGCAAGACCTTCGACAGCC
TGCCCGCGGTGCTCGAAGGCCGCCGCCACATCGTCCTCACGCGCGATGCCGACTGGCAGGAGGAAGGCGCCGAACCCGTC
GCAAGCGTCGAGGAAGCGCTGAAACGCGCCAATGCGCCGCATGTGATGGTGATCGGCGGCGCCGAAATCTATGCGCTGTT
CCTGCCGCTCGCCGACCGCATCGAACTGACCGAGGTGGGCCTCGAACCCGCTGGCGATGCGGTGATCGACTATCCCGATC
CGGCGCTCTGGCGCGAGGTCGCGCGGGTCGACCATCGAGCGGGCGAGGCGGGGCGCCCGGCCTACAGCTTTGTCACGCTC
GCGCGACGGAATGGCTAG

Upstream 100 bases:

>100_bases
ACGCCGCGCATGTGCATCATTATGGCAAGGCGGCGGTGCGCGACGGCCGCAAGCTGGGCCATGTCACGTGGGTCGGCTTT
GGGGTGGGAAACACACCGGC

Downstream 100 bases:

>100_bases
TCAAATGGCACCAAACAGCATCGTCGTTGCGAGAAGCGAAGCGACGCGGCAATCCAGAGCGCGCGTCAACCGCTGTGGAT
TGCTTCGCTTCGCTCGCAAT

Product: dihydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 165; Mature: 165

Protein sequence:

>165_residues
MNHPEITLILARAANGVIGAKGRMPWHLPADLRRFKQLTMGRPMIMGRKTFDSLPAVLEGRRHIVLTRDADWQEEGAEPV
ASVEEALKRANAPHVMVIGGAEIYALFLPLADRIELTEVGLEPAGDAVIDYPDPALWREVARVDHRAGEAGRPAYSFVTL
ARRNG

Sequences:

>Translated_165_residues
MNHPEITLILARAANGVIGAKGRMPWHLPADLRRFKQLTMGRPMIMGRKTFDSLPAVLEGRRHIVLTRDADWQEEGAEPV
ASVEEALKRANAPHVMVIGGAEIYALFLPLADRIELTEVGLEPAGDAVIDYPDPALWREVARVDHRAGEAGRPAYSFVTL
ARRNG
>Mature_165_residues
MNHPEITLILARAANGVIGAKGRMPWHLPADLRRFKQLTMGRPMIMGRKTFDSLPAVLEGRRHIVLTRDADWQEEGAEPV
ASVEEALKRANAPHVMVIGGAEIYALFLPLADRIELTEVGLEPAGDAVIDYPDPALWREVARVDHRAGEAGRPAYSFVTL
ARRNG

Specific function: Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis [H]

COG id: COG0262

COG function: function code H; Dihydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DHFR (dihydrofolate reductase) domain [H]

Homologues:

Organism=Escherichia coli, GI1786233, Length=159, Percent_Identity=38.9937106918239, Blast_Score=106, Evalue=6e-25,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 10,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012259
- InterPro:   IPR017925
- InterPro:   IPR001796 [H]

Pfam domain/function: PF00186 DHFR_1 [H]

EC number: =1.5.1.3 [H]

Molecular weight: Translated: 18208; Mature: 18208

Theoretical pI: Translated: 7.09; Mature: 7.09

Prosite motif: PS00075 DHFR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNHPEITLILARAANGVIGAKGRMPWHLPADLRRFKQLTMGRPMIMGRKTFDSLPAVLEG
CCCCCEEEEEEECCCCEEECCCCCCCCCCHHHHHHHHHHCCCCEEECCHHHHHHHHHHCC
RRHIVLTRDADWQEEGAEPVASVEEALKRANAPHVMVIGGAEIYALFLPLADRIELTEVG
CEEEEEECCCCCHHHCCCHHHHHHHHHHHCCCCEEEEEECHHEEHHHHCCCCCEEHHHCC
LEPAGDAVIDYPDPALWREVARVDHRAGEAGRPAYSFVTLARRNG
CCCCCCEEEECCCHHHHHHHHHHHHHCCCCCCCHHHHEEEEECCC
>Mature Secondary Structure
MNHPEITLILARAANGVIGAKGRMPWHLPADLRRFKQLTMGRPMIMGRKTFDSLPAVLEG
CCCCCEEEEEEECCCCEEECCCCCCCCCCHHHHHHHHHHCCCCEEECCHHHHHHHHHHCC
RRHIVLTRDADWQEEGAEPVASVEEALKRANAPHVMVIGGAEIYALFLPLADRIELTEVG
CEEEEEECCCCCHHHCCCHHHHHHHHHHHCCCCEEEEEECHHEEHHHHCCCCCEEHHHCC
LEPAGDAVIDYPDPALWREVARVDHRAGEAGRPAYSFVTLARRNG
CCCCCCEEEECCCHHHHHHHHHHHHHCCCCCCCHHHHEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6434541 [H]