Click here to switch to the map view.

The map label for this gene is mdh

Identifier: 103487711

GI number: 103487711

Start: 2343349

End: 2344311

Strand: Reverse

Name: mdh

Synonym: Sala_2230

Alternate gene names: 103487711

Gene position: 2344311-2343349 (Counterclockwise)

Preceding gene: 103487713

Following gene: 103487710

Centisome position: 70.08

GC content: 63.86

Gene sequence:

>963_bases
ATGGGACGCAAGAAGATCGCATTGATCGGAGCCGGGAATATCGGCGGAACGCTGGCGCTGCTCGCCGCGCAGAAGGAACT
CGGCGATGTCGTCCTGTTCGACGTCGTCGAAGGCGTGCCGCAGGGCAAGGCGCTCGACCTGTCGCAGGTCGGCCCGATCG
CGGGCTTTGACGCAAAGATCAGCGGCTCGAACGATTATGCCGACATCGCGGGTGCCGACGTCATCATCGTCACTGCGGGT
GTCGCTCGCAAGCCGGGGATGAGCCGCGACGATCTGCTCGGCATCAACCTGAAGGTCATGAAGGCCGTGGGCGAGGGCAT
CAAGGCCAACGCCCCCGACGCCTTTGTCATTTGCATCACCAATCCGCTGGACGCGATGGTTTGGGCTCTCCGTGAGTTCT
CGGGGCTGCCGCACAGCAAGGTCGTCGGCATGGCCGGCGTGCTCGATTCGGCGCGCTTCAGCCACTTCATCGCCGACGAA
TTCGACGTGTCGGTGAAGGATGTGAACACCTTCGTGCTCGGCGGTCATGGCGACACGATGGTTCCCGTCGTGCGTTATTC
GACCGTCAACGGCATCCCCGTTCCCGACCTCGTCAAGATGGGCCTGTCGAGCCAGGACAAGATCGACGCGATCGTCAAGC
GCACGCGCGGCGGCGGCGGCGAAATCGTCGCGCTGCTCGGCACCGGCTCGGCCTTCTATGCGCCCGCCGCCAGCGGCATC
GCGATGGCCGAAGCCTATCTGGGCGACCAGAAGCGCATCCTGCCCTGCGCCGCCTATGTCGATGGTCAGTATGGTGTTGA
CGGCCTCTATGTCGGCGTGCCGGTGATGATCGGTGCGGGCGGGGTCGAGAAGATCGTCGAGATCGAACTCGACGACGCCG
ACAAGGCGGGGCTTCAGGTTTCGGTCGACGCGGTCAAGGAACTGTTGGAAGCTTGCAAGAAGCTCGATCCCAGCCTCGCG
TAA

Upstream 100 bases:

>100_bases
GGGCGTCGCCAGTCTTGGGACCGGCAGCGGAGCTTTGGCCCGTGCTCCCGCCGGTCTGTGAACCAATGCCGGGCTTGCCA
GGAAGGGAGTGCCGCGCGCC

Downstream 100 bases:

>100_bases
ACAGCTTGCGCCCCTGCGAAGGCAGGGCCCGTCGCCCGCGCTCGACAGGTCGGACGGGCCGGTGAATCAAATATCTGGAG
ATGGGCCCCTGCCTTCGCAG

Product: malate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 320; Mature: 319

Protein sequence:

>320_residues
MGRKKIALIGAGNIGGTLALLAAQKELGDVVLFDVVEGVPQGKALDLSQVGPIAGFDAKISGSNDYADIAGADVIIVTAG
VARKPGMSRDDLLGINLKVMKAVGEGIKANAPDAFVICITNPLDAMVWALREFSGLPHSKVVGMAGVLDSARFSHFIADE
FDVSVKDVNTFVLGGHGDTMVPVVRYSTVNGIPVPDLVKMGLSSQDKIDAIVKRTRGGGGEIVALLGTGSAFYAPAASGI
AMAEAYLGDQKRILPCAAYVDGQYGVDGLYVGVPVMIGAGGVEKIVEIELDDADKAGLQVSVDAVKELLEACKKLDPSLA

Sequences:

>Translated_320_residues
MGRKKIALIGAGNIGGTLALLAAQKELGDVVLFDVVEGVPQGKALDLSQVGPIAGFDAKISGSNDYADIAGADVIIVTAG
VARKPGMSRDDLLGINLKVMKAVGEGIKANAPDAFVICITNPLDAMVWALREFSGLPHSKVVGMAGVLDSARFSHFIADE
FDVSVKDVNTFVLGGHGDTMVPVVRYSTVNGIPVPDLVKMGLSSQDKIDAIVKRTRGGGGEIVALLGTGSAFYAPAASGI
AMAEAYLGDQKRILPCAAYVDGQYGVDGLYVGVPVMIGAGGVEKIVEIELDDADKAGLQVSVDAVKELLEACKKLDPSLA
>Mature_319_residues
GRKKIALIGAGNIGGTLALLAAQKELGDVVLFDVVEGVPQGKALDLSQVGPIAGFDAKISGSNDYADIAGADVIIVTAGV
ARKPGMSRDDLLGINLKVMKAVGEGIKANAPDAFVICITNPLDAMVWALREFSGLPHSKVVGMAGVLDSARFSHFIADEF
DVSVKDVNTFVLGGHGDTMVPVVRYSTVNGIPVPDLVKMGLSSQDKIDAIVKRTRGGGGEIVALLGTGSAFYAPAASGIA
MAEAYLGDQKRILPCAAYVDGQYGVDGLYVGVPVMIGAGGVEKIVEIELDDADKAGLQVSVDAVKELLEACKKLDPSLA

Specific function: Catalyzes the reversible oxidation of malate to oxaloacetate

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. MDH type 3 family

Homologues:

Organism=Homo sapiens, GI47059044, Length=317, Percent_Identity=29.0220820189274, Blast_Score=151, Evalue=1e-36,
Organism=Homo sapiens, GI221136809, Length=317, Percent_Identity=29.0220820189274, Blast_Score=151, Evalue=1e-36,
Organism=Homo sapiens, GI291575128, Length=320, Percent_Identity=29.6875, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI4557032, Length=320, Percent_Identity=29.6875, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI9257228, Length=305, Percent_Identity=29.8360655737705, Blast_Score=138, Evalue=7e-33,
Organism=Homo sapiens, GI4504973, Length=305, Percent_Identity=29.8360655737705, Blast_Score=138, Evalue=7e-33,
Organism=Homo sapiens, GI5031857, Length=310, Percent_Identity=28.7096774193548, Blast_Score=136, Evalue=2e-32,
Organism=Homo sapiens, GI260099723, Length=307, Percent_Identity=28.6644951140065, Blast_Score=136, Evalue=3e-32,
Organism=Homo sapiens, GI15082234, Length=318, Percent_Identity=26.7295597484277, Blast_Score=135, Evalue=4e-32,
Organism=Homo sapiens, GI260099725, Length=200, Percent_Identity=31.5, Blast_Score=103, Evalue=1e-22,
Organism=Homo sapiens, GI260099727, Length=200, Percent_Identity=31.5, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI21735621, Length=293, Percent_Identity=29.3515358361775, Blast_Score=98, Evalue=8e-21,
Organism=Homo sapiens, GI207028494, Length=190, Percent_Identity=27.3684210526316, Blast_Score=83, Evalue=4e-16,
Organism=Homo sapiens, GI103472011, Length=312, Percent_Identity=19.8717948717949, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1789632, Length=319, Percent_Identity=31.6614420062696, Blast_Score=102, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI17535107, Length=290, Percent_Identity=26.2068965517241, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17554310, Length=293, Percent_Identity=35.8361774744027, Blast_Score=108, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6322765, Length=300, Percent_Identity=30.3333333333333, Blast_Score=99, Evalue=6e-22,
Organism=Saccharomyces cerevisiae, GI6324446, Length=268, Percent_Identity=29.4776119402985, Blast_Score=78, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6320125, Length=326, Percent_Identity=26.0736196319018, Blast_Score=77, Evalue=4e-15,
Organism=Drosophila melanogaster, GI17136226, Length=309, Percent_Identity=28.1553398058252, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24647881, Length=321, Percent_Identity=31.7757009345794, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI45550422, Length=312, Percent_Identity=27.5641025641026, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24663595, Length=284, Percent_Identity=30.6338028169014, Blast_Score=92, Evalue=6e-19,
Organism=Drosophila melanogaster, GI24663599, Length=317, Percent_Identity=26.813880126183, Blast_Score=86, Evalue=4e-17,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): MDH_SPHAL (Q1GQY3)

Other databases:

- EMBL:   CP000356
- RefSeq:   YP_617272.1
- ProteinModelPortal:   Q1GQY3
- SMR:   Q1GQY3
- GeneID:   4080301
- GenomeReviews:   CP000356_GR
- KEGG:   sal:Sala_2230
- NMPDR:   fig|317655.9.peg.2154
- HOGENOM:   HBG566126
- OMA:   ECTYVEG
- ProtClustDB:   PRK06223
- BioCyc:   SALA317655:SALA_2230-MONOMER
- BRENDA:   1.1.1.37
- GO:   GO:0005488
- GO:   GO:0006096
- HAMAP:   MF_00487
- InterPro:   IPR001557
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR011275
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.90.110.10
- Gene3D:   G3DSA:3.40.50.720
- PIRSF:   PIRSF000102
- PRINTS:   PR00086
- TIGRFAMs:   TIGR01763

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N; SSF56327 Lactate_DH/Glyco_hydro_4_C

EC number: =1.1.1.37

Molecular weight: Translated: 33009; Mature: 32877

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: NA

Important sites: ACT_SITE 176-176 BINDING 34-34 BINDING 83-83 BINDING 89-89 BINDING 96-96 BINDING 121-121 BINDING 152-152

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGRKKIALIGAGNIGGTLALLAAQKELGDVVLFDVVEGVPQGKALDLSQVGPIAGFDAKI
CCCEEEEEEECCCCCCHHHEEEHHHHHCCEEEEHHHCCCCCCCEECHHHCCCCCCCCEEE
SGSNDYADIAGADVIIVTAGVARKPGMSRDDLLGINLKVMKAVGEGIKANAPDAFVICIT
CCCCCCCHHCCCCEEEEECCCCCCCCCCCCCEEECHHHHHHHHCCCCCCCCCCEEEEEEC
NPLDAMVWALREFSGLPHSKVVGMAGVLDSARFSHFIADEFDVSVKDVNTFVLGGHGDTM
CCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECEEEEECCCCCCE
VPVVRYSTVNGIPVPDLVKMGLSSQDKIDAIVKRTRGGGGEIVALLGTGSAFYAPAASGI
EEEEEEECCCCCCCHHHHHHCCCCCHHHHHHHHHCCCCCCCEEEEEECCCEEECCCCCCH
AMAEAYLGDQKRILPCAAYVDGQYGVDGLYVGVPVMIGAGGVEKIVEIELDDADKAGLQV
HHHHHHHCCCCCEEEEEEEECCCCCCCEEEECCEEEECCCCCCEEEEEEECCCCCCCCEE
SVDAVKELLEACKKLDPSLA
EHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
GRKKIALIGAGNIGGTLALLAAQKELGDVVLFDVVEGVPQGKALDLSQVGPIAGFDAKI
CCEEEEEEECCCCCCHHHEEEHHHHHCCEEEEHHHCCCCCCCEECHHHCCCCCCCCEEE
SGSNDYADIAGADVIIVTAGVARKPGMSRDDLLGINLKVMKAVGEGIKANAPDAFVICIT
CCCCCCCHHCCCCEEEEECCCCCCCCCCCCCEEECHHHHHHHHCCCCCCCCCCEEEEEEC
NPLDAMVWALREFSGLPHSKVVGMAGVLDSARFSHFIADEFDVSVKDVNTFVLGGHGDTM
CCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECEEEEECCCCCCE
VPVVRYSTVNGIPVPDLVKMGLSSQDKIDAIVKRTRGGGGEIVALLGTGSAFYAPAASGI
EEEEEEECCCCCCCHHHHHHCCCCCHHHHHHHHHCCCCCCCEEEEEECCCEEECCCCCCH
AMAEAYLGDQKRILPCAAYVDGQYGVDGLYVGVPVMIGAGGVEKIVEIELDDADKAGLQV
HHHHHHHCCCCCEEEEEEEECCCCCCCEEEECCEEEECCCCCCEEEEEEECCCCCCCCEE
SVDAVKELLEACKKLDPSLA
EHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA