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The map label for this gene is sucB [H]

Identifier: 103487708

GI number: 103487708

Start: 2338186

End: 2339400

Strand: Reverse

Name: sucB [H]

Synonym: Sala_2227

Alternate gene names: 103487708

Gene position: 2339400-2338186 (Counterclockwise)

Preceding gene: 103487709

Following gene: 103487707

Centisome position: 69.93

GC content: 66.34

Gene sequence:

>1215_bases
ATGAGCACCGAAGTCAAAGTCCCCACGCTGGGCGAAAGCGTCACCGAAGCGACGATCGGCGAATGGCTGAAAAAGCCCGG
CGAAGCGGTCGCGCTCGACGAGCCCATCGCCAGCCTTGAAACCGACAAGGTCGCGGTCGAGGTGCCCTCGCCCGTCGCGG
GGGTGATGGGGCAGCAGCTCGCTGCGGTCGGCGACACGGTCAATGTCGGTGCGGTGATCGCGACGGTTGAAGCGGGCGAT
GCGGCGGCGGCGCCCGCCCCCGCGAAGGCCGAGACGACGGCGCCCGCCCCCGCCGCGACCGCCCCCGCCGCGGGTGCAGG
CATCGACACCGTTGCAACCATGTCGCCGGCGGTGCGCCGTCTCGTCCTCGAACACGGGCTCGATCCGACGAAGATCAAGG
GGACGGGGAAGGACGGTCGGCTGACCAAGGAAGATGTGCTCGCGGCGGCGAATGCGGCGCCCGAAACCCCGCCTGCTTCG
GCTCCCGCGCCGGCGGCAGCGCCCGCCGAGCCGGGCCGTCAGGAAGAGCGCGTCAAGATGACCCGCCTGCGCCAGACGAT
CGCCAAGCGGCTGAAAGCGGCGCAGGACACGGCGGCGATGCTGACGACCTTCAACGACGTCGACATGTCGGCGGTGATCG
AAGCGCGCGCGCGCTACAAGGATCTGTTCGAAAAGAAGCATGGCGTGCGGCTGGGCTTCATGGGCTTCTTCACCAAGGCG
GCGTGCCTCGCGCTCAAGGACATTCCCGCGGTCAACGGCCGCATCGACGGCGACGAGATCGTCTATAATGGTTATATGGA
CATTTCGGTCGCGGTGAGCGGGCCGAGCGGGCTTGTCGTGCCGGTGATCCGCAATGCCGAGACGCTGAGCTTCGCCGACA
TCGAAAAGACGATCGGTGATTTCGGCAAGCGCGCCAAGGAGGGCACGCTGACCATGGACGACATGGCGGGCGGCACCTTT
ACCATTTCGAACGGCGGCGTGTTCGGGTCGCTGATGTCGACCCCGATCATCAACCCGCCGCAGTCGGCGGTGCTCGGCCT
CCACCGCATCGAGGACCGTCCGGTCGCGATCAATGGTCAGGTCGTGATCCGTCCGATGATGTATCTGGCGCTCAGCTATG
ACCACCGCCTGATCGACGGCCGCGAGGCGGTGACCTTCCTCAAGACGATCAAGGAAGCGATCGAAGATCCGACGCGGTTG
CTGATCGATTTGTAA

Upstream 100 bases:

>100_bases
CGAACAGTCGGCGCTGGTCGCCGATGCGCTCGGCCTGTCGGTTCGCGCCGAAATCCGCCGCACCAAGAACAAAGCCTGAT
CCCCCTAAGGAACTGCATCT

Downstream 100 bases:

>100_bases
GGCCAAGTGAACTGCTGCGCACGCAGGAGATATTCGAGCTGTTCTCCTGCGAACGCAGGAGTCCAGGGTGGAGAGCAATG
ACGGTGCAACCAAACCCCGG

Product: 2-oxoglutarate dehydrogenase, E2 component, dihydrolipoamide succinyltransferase

Products: NA

Alternate protein names: 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 404; Mature: 403

Protein sequence:

>404_residues
MSTEVKVPTLGESVTEATIGEWLKKPGEAVALDEPIASLETDKVAVEVPSPVAGVMGQQLAAVGDTVNVGAVIATVEAGD
AAAAPAPAKAETTAPAPAATAPAAGAGIDTVATMSPAVRRLVLEHGLDPTKIKGTGKDGRLTKEDVLAAANAAPETPPAS
APAPAAAPAEPGRQEERVKMTRLRQTIAKRLKAAQDTAAMLTTFNDVDMSAVIEARARYKDLFEKKHGVRLGFMGFFTKA
ACLALKDIPAVNGRIDGDEIVYNGYMDISVAVSGPSGLVVPVIRNAETLSFADIEKTIGDFGKRAKEGTLTMDDMAGGTF
TISNGGVFGSLMSTPIINPPQSAVLGLHRIEDRPVAINGQVVIRPMMYLALSYDHRLIDGREAVTFLKTIKEAIEDPTRL
LIDL

Sequences:

>Translated_404_residues
MSTEVKVPTLGESVTEATIGEWLKKPGEAVALDEPIASLETDKVAVEVPSPVAGVMGQQLAAVGDTVNVGAVIATVEAGD
AAAAPAPAKAETTAPAPAATAPAAGAGIDTVATMSPAVRRLVLEHGLDPTKIKGTGKDGRLTKEDVLAAANAAPETPPAS
APAPAAAPAEPGRQEERVKMTRLRQTIAKRLKAAQDTAAMLTTFNDVDMSAVIEARARYKDLFEKKHGVRLGFMGFFTKA
ACLALKDIPAVNGRIDGDEIVYNGYMDISVAVSGPSGLVVPVIRNAETLSFADIEKTIGDFGKRAKEGTLTMDDMAGGTF
TISNGGVFGSLMSTPIINPPQSAVLGLHRIEDRPVAINGQVVIRPMMYLALSYDHRLIDGREAVTFLKTIKEAIEDPTRL
LIDL
>Mature_403_residues
STEVKVPTLGESVTEATIGEWLKKPGEAVALDEPIASLETDKVAVEVPSPVAGVMGQQLAAVGDTVNVGAVIATVEAGDA
AAAPAPAKAETTAPAPAATAPAAGAGIDTVATMSPAVRRLVLEHGLDPTKIKGTGKDGRLTKEDVLAAANAAPETPPASA
PAPAAAPAEPGRQEERVKMTRLRQTIAKRLKAAQDTAAMLTTFNDVDMSAVIEARARYKDLFEKKHGVRLGFMGFFTKAA
CLALKDIPAVNGRIDGDEIVYNGYMDISVAVSGPSGLVVPVIRNAETLSFADIEKTIGDFGKRAKEGTLTMDDMAGGTFT
ISNGGVFGSLMSTPIINPPQSAVLGLHRIEDRPVAINGQVVIRPMMYLALSYDHRLIDGREAVTFLKTIKEAIEDPTRLL
IDL

Specific function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components:2-oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and l

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI19923748, Length=254, Percent_Identity=62.992125984252, Blast_Score=324, Evalue=1e-88,
Organism=Homo sapiens, GI31711992, Length=437, Percent_Identity=29.9771167048055, Blast_Score=172, Evalue=4e-43,
Organism=Homo sapiens, GI110671329, Length=422, Percent_Identity=28.436018957346, Blast_Score=169, Evalue=6e-42,
Organism=Homo sapiens, GI203098753, Length=454, Percent_Identity=28.6343612334802, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI203098816, Length=454, Percent_Identity=28.6343612334802, Blast_Score=159, Evalue=4e-39,
Organism=Homo sapiens, GI260898739, Length=160, Percent_Identity=35, Blast_Score=98, Evalue=1e-20,
Organism=Escherichia coli, GI1786946, Length=404, Percent_Identity=53.7128712871287, Blast_Score=417, Evalue=1e-118,
Organism=Escherichia coli, GI1786305, Length=427, Percent_Identity=32.0843091334895, Blast_Score=178, Evalue=7e-46,
Organism=Caenorhabditis elegans, GI25146366, Length=437, Percent_Identity=43.2494279176201, Blast_Score=323, Evalue=1e-88,
Organism=Caenorhabditis elegans, GI17537937, Length=419, Percent_Identity=27.9236276849642, Blast_Score=181, Evalue=6e-46,
Organism=Caenorhabditis elegans, GI17560088, Length=438, Percent_Identity=29.4520547945205, Blast_Score=163, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI17538894, Length=305, Percent_Identity=33.1147540983607, Blast_Score=144, Evalue=8e-35,
Organism=Saccharomyces cerevisiae, GI6320352, Length=403, Percent_Identity=50.8684863523573, Blast_Score=377, Evalue=1e-105,
Organism=Saccharomyces cerevisiae, GI6324258, Length=457, Percent_Identity=26.9146608315098, Blast_Score=125, Evalue=9e-30,
Organism=Drosophila melanogaster, GI24645909, Length=225, Percent_Identity=61.3333333333333, Blast_Score=296, Evalue=1e-80,
Organism=Drosophila melanogaster, GI18859875, Length=422, Percent_Identity=29.6208530805687, Blast_Score=171, Evalue=9e-43,
Organism=Drosophila melanogaster, GI20129315, Length=231, Percent_Identity=28.5714285714286, Blast_Score=105, Evalue=7e-23,
Organism=Drosophila melanogaster, GI24582497, Length=231, Percent_Identity=28.5714285714286, Blast_Score=104, Evalue=9e-23,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053
- InterPro:   IPR006255 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.61 [H]

Molecular weight: Translated: 42226; Mature: 42095

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTEVKVPTLGESVTEATIGEWLKKPGEAVALDEPIASLETDKVAVEVPSPVAGVMGQQL
CCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCHHHCCCCEEEEECCCCHHHHHHHHH
AAVGDTVNVGAVIATVEAGDAAAAPAPAKAETTAPAPAATAPAAGAGIDTVATMSPAVRR
HHCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
LVLEHGLDPTKIKGTGKDGRLTKEDVLAAANAAPETPPASAPAPAAAPAEPGRQEERVKM
HHHHHCCCCCEEECCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
TRLRQTIAKRLKAAQDTAAMLTTFNDVDMSAVIEARARYKDLFEKKHGVRLGFMGFFTKA
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHH
ACLALKDIPAVNGRIDGDEIVYNGYMDISVAVSGPSGLVVPVIRNAETLSFADIEKTIGD
HHHHHHCCCCCCCCCCCCEEEEECEEEEEEEEECCCCEEEEEECCCCCCCHHHHHHHHHH
FGKRAKEGTLTMDDMAGGTFTISNGGVFGSLMSTPIINPPQSAVLGLHRIEDRPVAINGQ
HHHHHCCCCEEECCCCCCEEEEECCCEEHHHHHCCCCCCCHHHHHHHHHCCCCCEEECCC
VVIRPMMYLALSYDHRLIDGREAVTFLKTIKEAIEDPTRLLIDL
CHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCHHHEECC
>Mature Secondary Structure 
STEVKVPTLGESVTEATIGEWLKKPGEAVALDEPIASLETDKVAVEVPSPVAGVMGQQL
CCCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCHHHCCCCEEEEECCCCHHHHHHHHH
AAVGDTVNVGAVIATVEAGDAAAAPAPAKAETTAPAPAATAPAAGAGIDTVATMSPAVRR
HHCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
LVLEHGLDPTKIKGTGKDGRLTKEDVLAAANAAPETPPASAPAPAAAPAEPGRQEERVKM
HHHHHCCCCCEEECCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
TRLRQTIAKRLKAAQDTAAMLTTFNDVDMSAVIEARARYKDLFEKKHGVRLGFMGFFTKA
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHH
ACLALKDIPAVNGRIDGDEIVYNGYMDISVAVSGPSGLVVPVIRNAETLSFADIEKTIGD
HHHHHHCCCCCCCCCCCCEEEEECEEEEEEEEECCCCEEEEEECCCCCCCHHHHHHHHHH
FGKRAKEGTLTMDDMAGGTFTISNGGVFGSLMSTPIINPPQSAVLGLHRIEDRPVAINGQ
HHHHHCCCCEEECCCCCCEEEEECCCEEHHHHHCCCCCCCHHHHHHHHHCCCCCEEECCC
VVIRPMMYLALSYDHRLIDGREAVTFLKTIKEAIEDPTRLLIDL
CHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12874367 [H]