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The map label for this gene is queA

Identifier: 103487672

GI number: 103487672

Start: 2303438

End: 2304493

Strand: Reverse

Name: queA

Synonym: Sala_2191

Alternate gene names: 103487672

Gene position: 2304493-2303438 (Counterclockwise)

Preceding gene: 103487673

Following gene: 103487670

Centisome position: 68.89

GC content: 69.03

Gene sequence:

>1056_bases
ATGCGCGTCGACGCCTTCGACTTCGATCTGCCCAGCGAGCGCATCGCGCTGCGCCCGGCGCGACCGCGCGACGCGGCGCG
GATGCTGGTGGTCGACGCCGGGAATATCGTCGATGCCGGTGTGCGCGACCTGCCGCAATGGCTGCGGCCCGGCGACTGCC
TGGTGTTCAACGACACGCGCGTGATTCCGGCGCAGCTCGAAGGGCTGCGCGGCGAGGCGAAGATTGGCGCGACGCTGCAC
AAGCGCATCGACCTGCGCCGCTGGCAGGCGTTTGTGCGCAACGCCAAGCGGCTGCGCGTCGGCGAGACGGTCGATTTCGG
CTCGGGAGTCGCCGCGCTGGCCGAGGAACGGCTCGCCGACGGCAGCTTCGTCCTGGCCTTCGCCGGCGACGAGCCGGTCG
AGCTGCTGCTTGAACGCGCGGGCACGATGCCGCTGCCGCCCTATATCGCGGGCAAGCGCGGCGCCGACGAGGCCGACCGC
GACGATTATCAGACGATGTTCGCACGCGAAGACGGCGCGGTCGCAGCGCCGACCGCGGCGCTGCACTTCACCCCCGAACT
GCTCGCGGCGCTGGCGGCGGCGGGGATCGCGACCGAAACGCTGACGCTTCATGTCGGCGCGGGCACCTTCCTGCCGGTCA
AGGCCGACGACACCGACGATCACGTCATGCACGCCGAATGGGGACGGATCGAGGCCGACACCGCGGCGCGGCTCAACGCG
GTGCGCGCGAGCGGCGGCCGGGTGATCGCAGTGGGCACGACCAGTCTGCGCCTGCTCGAAAGCGCCGCCCGCGACGATGG
CACCATCACACCCTTCGCGGGCGACACCTCGATTTTCATCACGCCCGGTTATCGGTTCAAGGCGATCGACGGGCTGATGA
CCAATTTCCACCTGCCGCGCTCGACGCTGTTCATGCTGGTCAGCGCCCTGATGGGGCTGGAAACGATGCAGGCGGCCTAT
GCCCATGCGATTGCACGCGAATATCGCTTCTACAGCTATGGCGATGCGAGCCTTCTGTTGCCGCGTCAGAGCGGCCGTTC
GCGCACCCTGTCTTGA

Upstream 100 bases:

>100_bases
GATGCTCACCGAAGCGCAGCCCGCAGCATCCGCTGACGTGACGATCGACGAACTCAACGCGCCTATCGCGCAATAATCGC
GGAAAGCCGCGCGAACCAAT

Downstream 100 bases:

>100_bases
TCGGGCGCCACTTCATCGGAGGTGCCGCAGCCTGAGGGGCAGATATACAGCACGCCGCTCTATATTTCGATCGTCACCCG
CACCCGGTCGACGCCGACGC

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA

Number of amino acids: Translated: 351; Mature: 351

Protein sequence:

>351_residues
MRVDAFDFDLPSERIALRPARPRDAARMLVVDAGNIVDAGVRDLPQWLRPGDCLVFNDTRVIPAQLEGLRGEAKIGATLH
KRIDLRRWQAFVRNAKRLRVGETVDFGSGVAALAEERLADGSFVLAFAGDEPVELLLERAGTMPLPPYIAGKRGADEADR
DDYQTMFAREDGAVAAPTAALHFTPELLAALAAAGIATETLTLHVGAGTFLPVKADDTDDHVMHAEWGRIEADTAARLNA
VRASGGRVIAVGTTSLRLLESAARDDGTITPFAGDTSIFITPGYRFKAIDGLMTNFHLPRSTLFMLVSALMGLETMQAAY
AHAIAREYRFYSYGDASLLLPRQSGRSRTLS

Sequences:

>Translated_351_residues
MRVDAFDFDLPSERIALRPARPRDAARMLVVDAGNIVDAGVRDLPQWLRPGDCLVFNDTRVIPAQLEGLRGEAKIGATLH
KRIDLRRWQAFVRNAKRLRVGETVDFGSGVAALAEERLADGSFVLAFAGDEPVELLLERAGTMPLPPYIAGKRGADEADR
DDYQTMFAREDGAVAAPTAALHFTPELLAALAAAGIATETLTLHVGAGTFLPVKADDTDDHVMHAEWGRIEADTAARLNA
VRASGGRVIAVGTTSLRLLESAARDDGTITPFAGDTSIFITPGYRFKAIDGLMTNFHLPRSTLFMLVSALMGLETMQAAY
AHAIAREYRFYSYGDASLLLPRQSGRSRTLS
>Mature_351_residues
MRVDAFDFDLPSERIALRPARPRDAARMLVVDAGNIVDAGVRDLPQWLRPGDCLVFNDTRVIPAQLEGLRGEAKIGATLH
KRIDLRRWQAFVRNAKRLRVGETVDFGSGVAALAEERLADGSFVLAFAGDEPVELLLERAGTMPLPPYIAGKRGADEADR
DDYQTMFAREDGAVAAPTAALHFTPELLAALAAAGIATETLTLHVGAGTFLPVKADDTDDHVMHAEWGRIEADTAARLNA
VRASGGRVIAVGTTSLRLLESAARDDGTITPFAGDTSIFITPGYRFKAIDGLMTNFHLPRSTLFMLVSALMGLETMQAAY
AHAIAREYRFYSYGDASLLLPRQSGRSRTLS

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA)

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family

Homologues:

Organism=Escherichia coli, GI1786606, Length=345, Percent_Identity=44.6376811594203, Blast_Score=269, Evalue=2e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEA_SPHAL (Q1GR22)

Other databases:

- EMBL:   CP000356
- RefSeq:   YP_617233.1
- ProteinModelPortal:   Q1GR22
- SMR:   Q1GR22
- GeneID:   4080225
- GenomeReviews:   CP000356_GR
- KEGG:   sal:Sala_2191
- NMPDR:   fig|317655.9.peg.2120
- HOGENOM:   HBG286076
- OMA:   ITRNPAP
- ProtClustDB:   PRK00147
- BioCyc:   SALA317655:SALA_2191-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00113
- InterPro:   IPR003699
- TIGRFAMs:   TIGR00113

Pfam domain/function: PF02547 Queuosine_synth; SSF111337 Queuosine_synth

EC number: 5.-.-.- [C]

Molecular weight: Translated: 38051; Mature: 38051

Theoretical pI: Translated: 5.94; Mature: 5.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVDAFDFDLPSERIALRPARPRDAARMLVVDAGNIVDAGVRDLPQWLRPGDCLVFNDTR
CCCCCCCCCCCCCCEEECCCCCCCCEEEEEEECCCCHHHHHHHHHHHCCCCCEEEECCCE
VIPAQLEGLRGEAKIGATLHKRIDLRRWQAFVRNAKRLRVGETVDFGSGVAALAEERLAD
EEHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEECCCEECCCCCHHHHHHHHCCC
GSFVLAFAGDEPVELLLERAGTMPLPPYIAGKRGADEADRDDYQTMFAREDGAVAAPTAA
CCEEEEECCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCEECCCEE
LHFTPELLAALAAAGIATETLTLHVGAGTFLPVKADDTDDHVMHAEWGRIEADTAARLNA
EEECHHHHHHHHHHCCEEEEEEEEECCCEEEEEECCCCCCCEEECCCCCEECCHHHHHHH
VRASGGRVIAVGTTSLRLLESAARDDGTITPFAGDTSIFITPGYRFKAIDGLMTNFHLPR
EECCCCEEEEECCHHHHHHHHHHCCCCCEEECCCCCEEEEECCCEEEHHHHHHHHCCCCH
STLFMLVSALMGLETMQAAYAHAIAREYRFYSYGDASLLLPRQSGRSRTLS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEECCCEEEEECCCCCCCCCC
>Mature Secondary Structure
MRVDAFDFDLPSERIALRPARPRDAARMLVVDAGNIVDAGVRDLPQWLRPGDCLVFNDTR
CCCCCCCCCCCCCCEEECCCCCCCCEEEEEEECCCCHHHHHHHHHHHCCCCCEEEECCCE
VIPAQLEGLRGEAKIGATLHKRIDLRRWQAFVRNAKRLRVGETVDFGSGVAALAEERLAD
EEHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEECCCEECCCCCHHHHHHHHCCC
GSFVLAFAGDEPVELLLERAGTMPLPPYIAGKRGADEADRDDYQTMFAREDGAVAAPTAA
CCEEEEECCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCEECCCEE
LHFTPELLAALAAAGIATETLTLHVGAGTFLPVKADDTDDHVMHAEWGRIEADTAARLNA
EEECHHHHHHHHHHCCEEEEEEEEECCCEEEEEECCCCCCCEEECCCCCEECCHHHHHHH
VRASGGRVIAVGTTSLRLLESAARDDGTITPFAGDTSIFITPGYRFKAIDGLMTNFHLPR
EECCCCEEEEECCHHHHHHHHHHCCCCCEEECCCCCEEEEECCCEEEHHHHHHHHCCCCH
STLFMLVSALMGLETMQAAYAHAIAREYRFYSYGDASLLLPRQSGRSRTLS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEECCCEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA