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The map label for this gene is tgt

Identifier: 103487668

GI number: 103487668

Start: 2298168

End: 2299295

Strand: Reverse

Name: tgt

Synonym: Sala_2187

Alternate gene names: 103487668

Gene position: 2299295-2298168 (Counterclockwise)

Preceding gene: 103487669

Following gene: 103487667

Centisome position: 68.73

GC content: 67.64

Gene sequence:

>1128_bases
ATGAAGCCAAGATTCGCCTTTTCCATCTCCGCGACCGACGGCGCCGCGCGCGCAGGCGTGATCGCCATGCAGCGCGGCGA
AATCCGCACCCCTGCCTTCATGCCCGTCGGCACGGCGGCGACGGTGAAGGCGATGCGCCCCGCCGAGGTGCGCGCGGCGG
GCGCCGACATCATCCTCGGCAACACCTATCATCTGATGCTGCGGCCCACTGCCGAACGCATGGCGCGGTTCGGCGGCCTT
CACAAGTTCATGGGCTGGGACCGCCCGATCCTGACCGACAGCGGTGGTTATCAGGTGATGAGCCTGTCGGCGCTGACCAA
GCAGAGCGAAGAGGGCGTCGCGTTCAAGAGCCATCTCGACGGATCGCGCCACATGCTCACCCCCGAACGCTCGATGGAAA
TCCAGCGGCTGCTCGGCAGCGACATCGTGATGGCGTTCGACGAATGCCCGCCCGCCGGGGTGGACGCCCGGCGTGCCGAG
GCGAGCATGGAACGCTCGATGCGCTGGGCGGCGCGCAGCCGCGCGGGCTTCGACGCGGGGGAGGAGCATGCGGCGCGATC
GGCGCTGTTCGGCATCCAGCAGGGCTCGCTCGACGAAAAATTGCGTGCGCGCTCGGCCGCGACGCTCATCGACATCGGCT
TCGACGGCTATGCGATCGGTGGCCTGGCGGTGGGCGAGGGGCAGGCGGCGATGTTCGGCGTGCTCGACTTTGCACCGGCG
CAGCTGCCCGCCGACCGTCCGCGCTATCTGATGGGCGTTGGCAAGCCCGACGATCTGGTCGGCGCGGTCGCGCGCGGGGT
CGACATGTTCGATTGCGTGCTGCCGACCCGGTCGGGCCGCAACGGCCAGGCGTTCACGTGGGACGGTCCGCTCAACATCC
GCAACGCCAAATTCGCCGACGATCAGGAACCGCTCGATGCGTCGTGCGGCTGTCCCGTCTGCACGATCTGGTCGCGCGGC
TATCTGCATCACCTCGTCCGCGCCGGCGAAATGCTCGGCGCGATGCTGATGACCCAGCATAACATTCATTTTTATCAGGA
TCTGATGCAGGCGATGCGCGACGCGATCACCACGGGGCGTTTCGCGGCGTTCCGGTCCGATTTTGCCGCGCGCTATCGTC
GCGCCTGA

Upstream 100 bases:

>100_bases
CGCAAGGCCGATGGCCCCCACCCCAACCCCTCCCCCTGAAGGGGAGGGGCTTTTTTGTCCGCCTCGACTTTCGCGTTCCC
GCCCGCCATAGGCGCGCGCG

Downstream 100 bases:

>100_bases
TCAGTCGAGCTTGCCGAGCAGGTCGAGCAACGACTGCGGAATCGCCTCGTCAACCGTTGATTCATACGCCCTGCGCAGGG
CGCCATTGACGTCCTGTCCC

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase

Number of amino acids: Translated: 375; Mature: 375

Protein sequence:

>375_residues
MKPRFAFSISATDGAARAGVIAMQRGEIRTPAFMPVGTAATVKAMRPAEVRAAGADIILGNTYHLMLRPTAERMARFGGL
HKFMGWDRPILTDSGGYQVMSLSALTKQSEEGVAFKSHLDGSRHMLTPERSMEIQRLLGSDIVMAFDECPPAGVDARRAE
ASMERSMRWAARSRAGFDAGEEHAARSALFGIQQGSLDEKLRARSAATLIDIGFDGYAIGGLAVGEGQAAMFGVLDFAPA
QLPADRPRYLMGVGKPDDLVGAVARGVDMFDCVLPTRSGRNGQAFTWDGPLNIRNAKFADDQEPLDASCGCPVCTIWSRG
YLHHLVRAGEMLGAMLMTQHNIHFYQDLMQAMRDAITTGRFAAFRSDFAARYRRA

Sequences:

>Translated_375_residues
MKPRFAFSISATDGAARAGVIAMQRGEIRTPAFMPVGTAATVKAMRPAEVRAAGADIILGNTYHLMLRPTAERMARFGGL
HKFMGWDRPILTDSGGYQVMSLSALTKQSEEGVAFKSHLDGSRHMLTPERSMEIQRLLGSDIVMAFDECPPAGVDARRAE
ASMERSMRWAARSRAGFDAGEEHAARSALFGIQQGSLDEKLRARSAATLIDIGFDGYAIGGLAVGEGQAAMFGVLDFAPA
QLPADRPRYLMGVGKPDDLVGAVARGVDMFDCVLPTRSGRNGQAFTWDGPLNIRNAKFADDQEPLDASCGCPVCTIWSRG
YLHHLVRAGEMLGAMLMTQHNIHFYQDLMQAMRDAITTGRFAAFRSDFAARYRRA
>Mature_375_residues
MKPRFAFSISATDGAARAGVIAMQRGEIRTPAFMPVGTAATVKAMRPAEVRAAGADIILGNTYHLMLRPTAERMARFGGL
HKFMGWDRPILTDSGGYQVMSLSALTKQSEEGVAFKSHLDGSRHMLTPERSMEIQRLLGSDIVMAFDECPPAGVDARRAE
ASMERSMRWAARSRAGFDAGEEHAARSALFGIQQGSLDEKLRARSAATLIDIGFDGYAIGGLAVGEGQAAMFGVLDFAPA
QLPADRPRYLMGVGKPDDLVGAVARGVDMFDCVLPTRSGRNGQAFTWDGPLNIRNAKFADDQEPLDASCGCPVCTIWSRG
YLHHLVRAGEMLGAMLMTQHNIHFYQDLMQAMRDAITTGRFAAFRSDFAARYRRA

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family

Homologues:

Organism=Homo sapiens, GI13654276, Length=353, Percent_Identity=43.6260623229462, Blast_Score=297, Evalue=1e-80,
Organism=Escherichia coli, GI1786607, Length=368, Percent_Identity=55.7065217391304, Blast_Score=409, Evalue=1e-115,
Organism=Caenorhabditis elegans, GI17544680, Length=367, Percent_Identity=39.7820163487738, Blast_Score=254, Evalue=4e-68,
Organism=Caenorhabditis elegans, GI25146502, Length=201, Percent_Identity=26.865671641791, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI19920510, Length=377, Percent_Identity=43.5013262599469, Blast_Score=317, Evalue=6e-87,
Organism=Drosophila melanogaster, GI21355311, Length=153, Percent_Identity=25.4901960784314, Blast_Score=79, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TGT_SPHAL (Q1GR26)

Other databases:

- EMBL:   CP000356
- RefSeq:   YP_617229.1
- ProteinModelPortal:   Q1GR26
- SMR:   Q1GR26
- GeneID:   4080221
- GenomeReviews:   CP000356_GR
- KEGG:   sal:Sala_2187
- NMPDR:   fig|317655.9.peg.2116
- HOGENOM:   HBG629929
- OMA:   FMPVGTV
- ProtClustDB:   PRK00112
- BioCyc:   SALA317655:SALA_2187-MONOMER
- BRENDA:   2.4.2.29
- HAMAP:   MF_00168
- InterPro:   IPR004803
- InterPro:   IPR002616
- Gene3D:   G3DSA:3.20.20.105
- PANTHER:   PTHR11962
- TIGRFAMs:   TIGR00430
- TIGRFAMs:   TIGR00449

Pfam domain/function: PF01702 TGT; SSF51713 tRNA_ribo_trans

EC number: =2.4.2.29

Molecular weight: Translated: 40786; Mature: 40786

Theoretical pI: Translated: 8.49; Mature: 8.49

Prosite motif: NA

Important sites: ACT_SITE 93-93 BINDING 94-94

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
5.6 %Met     (Translated Protein)
6.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
5.6 %Met     (Mature Protein)
6.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPRFAFSISATDGAARAGVIAMQRGEIRTPAFMPVGTAATVKAMRPAEVRAAGADIILG
CCCCEEEEEECCCCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHCCCHHHHHCCCCEEEC
NTYHLMLRPTAERMARFGGLHKFMGWDRPILTDSGGYQVMSLSALTKQSEEGVAFKSHLD
CEEEEEECCHHHHHHHHCCHHHHHCCCCCEEECCCCCEEEEEHHHHCCCCCCCEEHHHCC
GSRHMLTPERSMEIQRLLGSDIVMAFDECPPAGVDARRAEASMERSMRWAARSRAGFDAG
CCCCCCCCHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCC
EEHAARSALFGIQQGSLDEKLRARSAATLIDIGFDGYAIGGLAVGEGQAAMFGVLDFAPA
HHHHHHHHHHHCCCCCHHHHHHHHCCCEEEEECCCCEEECCEEECCCCEEEEEHHHCCCC
QLPADRPRYLMGVGKPDDLVGAVARGVDMFDCVLPTRSGRNGQAFTWDGPLNIRNAKFAD
CCCCCCCCEEEECCCCHHHHHHHHHCCCHHHHCCCCCCCCCCCEEEECCCCCCCCCCCCC
DQEPLDASCGCPVCTIWSRGYLHHLVRAGEMLGAMLMTQHNIHFYQDLMQAMRDAITTGR
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
FAAFRSDFAARYRRA
HHHHHHHHHHHHCCC
>Mature Secondary Structure
MKPRFAFSISATDGAARAGVIAMQRGEIRTPAFMPVGTAATVKAMRPAEVRAAGADIILG
CCCCEEEEEECCCCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHCCCHHHHHCCCCEEEC
NTYHLMLRPTAERMARFGGLHKFMGWDRPILTDSGGYQVMSLSALTKQSEEGVAFKSHLD
CEEEEEECCHHHHHHHHCCHHHHHCCCCCEEECCCCCEEEEEHHHHCCCCCCCEEHHHCC
GSRHMLTPERSMEIQRLLGSDIVMAFDECPPAGVDARRAEASMERSMRWAARSRAGFDAG
CCCCCCCCHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCC
EEHAARSALFGIQQGSLDEKLRARSAATLIDIGFDGYAIGGLAVGEGQAAMFGVLDFAPA
HHHHHHHHHHHCCCCCHHHHHHHHCCCEEEEECCCCEEECCEEECCCCEEEEEHHHCCCC
QLPADRPRYLMGVGKPDDLVGAVARGVDMFDCVLPTRSGRNGQAFTWDGPLNIRNAKFAD
CCCCCCCCEEEECCCCHHHHHHHHHCCCHHHHCCCCCCCCCCCEEEECCCCCCCCCCCCC
DQEPLDASCGCPVCTIWSRGYLHHLVRAGEMLGAMLMTQHNIHFYQDLMQAMRDAITTGR
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
FAAFRSDFAARYRRA
HHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA