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The map label for this gene is dapE

Identifier: 103487502

GI number: 103487502

Start: 2129149

End: 2130282

Strand: Reverse

Name: dapE

Synonym: Sala_2019

Alternate gene names: 103487502

Gene position: 2130282-2129149 (Counterclockwise)

Preceding gene: 103487504

Following gene: 103487498

Centisome position: 63.68

GC content: 65.87

Gene sequence:

>1134_bases
ATGACCCGCAATATCGACCCCATCGAGCTAGCCAAGGCGCTGATCGCCGTCCCCTCCGTCACCCCGGCAACAGGGGCGGT
GTTCGACGTACTCGAGGCGGCGCTCACGCCGCTCGGGTTCACGGTCGAGCGGTTTATCGACGGGATCGAACCCGACGGGC
CGGTTGAAAACCTGCTCGCGGTACGACGCGGCAGCGGGCCAAAGCATTTTGGCTTTGCGGGGCATCTCGACGTCGTGCCG
CCGGGGGTTGGCTGGACAAGCGATGCCTTCGCGCCCGAAATACGCGGCGAACTGCTTTACGGGCGCGGCGCGGTCGACAT
GAAAGGGGCAATCGCCGCCTTTGTCGCCGCGGCCGCCGCCACCCCGGTCGATGCCGGGACGATCAGCCTGATCATCACCG
GCGACGAGGAAGGCCCGGCAATCTTTGGCACCCGCGCGCTGATGGAGCATATGGACGCGCGCCGCGTGACGCCCGACATG
ATCGTCGTCGGCGAGCCGACCTCGGTGAACCGGCTGGGTGACATGGTGAAGATCGGGCGGCGCGGATCGGTCAATATCTG
GATCGACGTGCCGGGGACGCAGGGGCATGTCGCTTACCCCCACCTGGCCGACAATCCGATCCCCAAGCTGGTGAAGATAT
TGGCGGCGATCGACGCGGTGGTGCTGGACGAAGGCAGCGACTGGTTCCAGCCGTCGAATATCGAGTTCACCGACATCGAG
GTCGGCAACGGCGCGACCAATGTCATTCCGGCGTCGGCGCGCGCGCGGCTGTCGATCCGCTTCAACGACCAGCACCGGGG
CGCCGAGCTGGTGGCGATGATCGAGCGCATCGCGCACGAGGTCGAACCCGCGGCCAGGGTGCTGGGCAAGATTTCGGGCG
AGGCCTTTCTGACGCCGCCGGGCGAATTGTCCGAACTGGTCGCCGAGGCGATCCATGCCGAGACCGACATCTGCGCCGAA
ATGTCGACGACGGGCGGCACCTCCGACGCGCGCTTCCTGCACGCGCTGTGTCCGGTCGTGGAGTTCGGGCTGACCAACGC
AACGATGCACAAGCTCGACGAAGCGGTGGCGATCGAGGACCTGCACAGGCTGACCGCAATCTATCGCGGGATCTTAATGC
GCGTTTTCCTCTAA

Upstream 100 bases:

>100_bases
GCATGATGCAGTCCTGCTTTCTGTTCTTCCTTTGTTTCGCACAATCGGCGCGCGATGCAAAGCCTCGACTTCGCGTCGTG
CGAAGGCGTAAAGGGCAGGG

Downstream 100 bases:

>100_bases
TCCTCGCGGCTGCGCCGGAGGATCACATAGACCGCGCCCGCGCCGCCGTGGCTGATATGCGCGGGGCGCAGCGCGACAAT
CCGATCGGCGTGGGGCGAGA

Product: succinyl-diaminopimelate desuccinylase

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase

Number of amino acids: Translated: 377; Mature: 376

Protein sequence:

>377_residues
MTRNIDPIELAKALIAVPSVTPATGAVFDVLEAALTPLGFTVERFIDGIEPDGPVENLLAVRRGSGPKHFGFAGHLDVVP
PGVGWTSDAFAPEIRGELLYGRGAVDMKGAIAAFVAAAAATPVDAGTISLIITGDEEGPAIFGTRALMEHMDARRVTPDM
IVVGEPTSVNRLGDMVKIGRRGSVNIWIDVPGTQGHVAYPHLADNPIPKLVKILAAIDAVVLDEGSDWFQPSNIEFTDIE
VGNGATNVIPASARARLSIRFNDQHRGAELVAMIERIAHEVEPAARVLGKISGEAFLTPPGELSELVAEAIHAETDICAE
MSTTGGTSDARFLHALCPVVEFGLTNATMHKLDEAVAIEDLHRLTAIYRGILMRVFL

Sequences:

>Translated_377_residues
MTRNIDPIELAKALIAVPSVTPATGAVFDVLEAALTPLGFTVERFIDGIEPDGPVENLLAVRRGSGPKHFGFAGHLDVVP
PGVGWTSDAFAPEIRGELLYGRGAVDMKGAIAAFVAAAAATPVDAGTISLIITGDEEGPAIFGTRALMEHMDARRVTPDM
IVVGEPTSVNRLGDMVKIGRRGSVNIWIDVPGTQGHVAYPHLADNPIPKLVKILAAIDAVVLDEGSDWFQPSNIEFTDIE
VGNGATNVIPASARARLSIRFNDQHRGAELVAMIERIAHEVEPAARVLGKISGEAFLTPPGELSELVAEAIHAETDICAE
MSTTGGTSDARFLHALCPVVEFGLTNATMHKLDEAVAIEDLHRLTAIYRGILMRVFL
>Mature_376_residues
TRNIDPIELAKALIAVPSVTPATGAVFDVLEAALTPLGFTVERFIDGIEPDGPVENLLAVRRGSGPKHFGFAGHLDVVPP
GVGWTSDAFAPEIRGELLYGRGAVDMKGAIAAFVAAAAATPVDAGTISLIITGDEEGPAIFGTRALMEHMDARRVTPDMI
VVGEPTSVNRLGDMVKIGRRGSVNIWIDVPGTQGHVAYPHLADNPIPKLVKILAAIDAVVLDEGSDWFQPSNIEFTDIEV
GNGATNVIPASARARLSIRFNDQHRGAELVAMIERIAHEVEPAARVLGKISGEAFLTPPGELSELVAEAIHAETDICAEM
STTGGTSDARFLHALCPVVEFGLTNATMHKLDEAVAIEDLHRLTAIYRGILMRVFL

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily

Homologues:

Organism=Escherichia coli, GI1788816, Length=381, Percent_Identity=39.3700787401575, Blast_Score=242, Evalue=3e-65,
Organism=Escherichia coli, GI1790395, Length=368, Percent_Identity=26.9021739130435, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1789236, Length=233, Percent_Identity=28.755364806867, Blast_Score=66, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17551016, Length=216, Percent_Identity=30.5555555555556, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPE_SPHAL (Q1GRJ2)

Other databases:

- EMBL:   CP000356
- RefSeq:   YP_617063.1
- ProteinModelPortal:   Q1GRJ2
- SMR:   Q1GRJ2
- MEROPS:   M20.010
- GeneID:   4079956
- GenomeReviews:   CP000356_GR
- KEGG:   sal:Sala_2019
- NMPDR:   fig|317655.9.peg.1950
- HOGENOM:   HBG728841
- OMA:   PMVFEDT
- ProtClustDB:   PRK13009
- BioCyc:   SALA317655:SALA_2019-MONOMER
- GO:   GO:0006508
- HAMAP:   MF_01690
- InterPro:   IPR001261
- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650
- TIGRFAMs:   TIGR01246

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20; SSF55031 Peptidase_M20_dimer

EC number: =3.5.1.18

Molecular weight: Translated: 40049; Mature: 39918

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: PS00758 ARGE_DAPE_CPG2_1; PS00759 ARGE_DAPE_CPG2_2

Important sites: ACT_SITE 77-77 ACT_SITE 136-136

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRNIDPIELAKALIAVPSVTPATGAVFDVLEAALTPLGFTVERFIDGIEPDGPVENLLA
CCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCHHHHHHH
VRRGSGPKHFGFAGHLDVVPPGVGWTSDAFAPEIRGELLYGRGAVDMKGAIAAFVAAAAA
HHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCEEEECCCCCCHHHHHHHHHHHHHC
TPVDAGTISLIITGDEEGPAIFGTRALMEHMDARRVTPDMIVVGEPTSVNRLGDMVKIGR
CCCCCCEEEEEEECCCCCCEEEHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHCC
RGSVNIWIDVPGTQGHVAYPHLADNPIPKLVKILAAIDAVVLDEGSDWFQPSNIEFTDIE
CCCEEEEEECCCCCCCEECCCCCCCCHHHHHHHHHHHHHHEEECCCCCCCCCCCEEEEEE
VGNGATNVIPASARARLSIRFNDQHRGAELVAMIERIAHEVEPAARVLGKISGEAFLTPP
ECCCCCCEEECCCCEEEEEEECCCCCCHHHHHHHHHHHHHHCHHHHHHHHCCCCEEECCC
GELSELVAEAIHAETDICAEMSTTGGTSDARFLHALCPVVEFGLTNATMHKLDEAVAIED
HHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
LHRLTAIYRGILMRVFL
HHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TRNIDPIELAKALIAVPSVTPATGAVFDVLEAALTPLGFTVERFIDGIEPDGPVENLLA
CCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCHHHHHHH
VRRGSGPKHFGFAGHLDVVPPGVGWTSDAFAPEIRGELLYGRGAVDMKGAIAAFVAAAAA
HHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCEEEECCCCCCHHHHHHHHHHHHHC
TPVDAGTISLIITGDEEGPAIFGTRALMEHMDARRVTPDMIVVGEPTSVNRLGDMVKIGR
CCCCCCEEEEEEECCCCCCEEEHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHCC
RGSVNIWIDVPGTQGHVAYPHLADNPIPKLVKILAAIDAVVLDEGSDWFQPSNIEFTDIE
CCCEEEEEECCCCCCCEECCCCCCCCHHHHHHHHHHHHHHEEECCCCCCCCCCCEEEEEE
VGNGATNVIPASARARLSIRFNDQHRGAELVAMIERIAHEVEPAARVLGKISGEAFLTPP
ECCCCCCEEECCCCEEEEEEECCCCCCHHHHHHHHHHHHHHCHHHHHHHHCCCCEEECCC
GELSELVAEAIHAETDICAEMSTTGGTSDARFLHALCPVVEFGLTNATMHKLDEAVAIED
HHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
LHRLTAIYRGILMRVFL
HHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA