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The map label for this gene is asnB [H]

Identifier: 103487403

GI number: 103487403

Start: 2021312

End: 2023207

Strand: Reverse

Name: asnB [H]

Synonym: Sala_1919

Alternate gene names: 103487403

Gene position: 2023207-2021312 (Counterclockwise)

Preceding gene: 103487404

Following gene: 103487402

Centisome position: 60.48

GC content: 67.83

Gene sequence:

>1896_bases
ATGTGCGGCATCGCGGGCATCTATCATCTTGAGACCGCGAAACCGGTCGATCCGGCGCGGCTGCGCGCGATGCTCCAGCC
GATGCAGCATCGCGGCCCCGACGGGTCGGGCGAATGGACCGCGCCCGGCGTCGGGCTGGCGCACCTGCGCCTCTCGATCA
TCGACATTGCGGGCAGCCCGCAGCCGATGGCGAGCGACGATGAGGCGGTGACGCTCACCTACAACGGCGAAATCTATAAC
TTCCGCGAACTGCGCGCCGAACTCGAGGATCGCGGGCACCGTTTCCGCACCAGCGGCGATACCGAGGTCATCATCGCGGC
GTGGCGGCAATGGGGGCCCGAATGCCTGTCGCGGCTCAACGGCATGTTCGCCTTTGCGATCCACGACCATGCGCGCGGCT
GCCTGTTCCTCGCGCGCGACCGGCTGGGGGTGAAGCCGCTGCACTATGCGCGGCTGGCGGACGGATCGGTGGCGTTCGCG
TCGGAACTGAAAGGGTTGCTGCGCCATCCCCTGCTGCGGCAGGAAGCCAGTTTCTCCGCGGTCGAGGATTTCTTCGCGCT
CGGCTATGTCCCCGACGACAATTGCATCGTTGCGGGGATCGAGAAGCTGCCCGCCGGGCATTATCTGATGCTCGAACGCG
GCAAGGGCGTGCCCGCGCCGACCTGCTGGTGGGCGCCCGATTTTTCGCGGCGGATCAAGGCGAGCGAGGGCGAGGCGGCC
GAACATCTGGTTTATCTGATGCGCGCCGCCGTGACCGACCGCATGGTCGCCGACGTGCCCTTGGGCGCCTTTCTGTCGGG
CGGGGTCGATTCGAGCGCGGTCGTCGCGCTGATGGCCGAGGCGAGCGCGAAGGCGGTCAAGACCTGCACGATCGGGTTCG
ACCAAGCGGCGCTCGACGAGACGGCCTATGCGCAGCAAATCGCCGAGCGCTTCGCGACCGATCACCGCACGCGCACGGTG
GCGGCGGGCGATTTCGCGCTGATCGACCGGATCGCCGACATGTTCGACGAACCCTTTGCCGACGCGAGCGCGCTGCCGAC
GTACCGGGTGTGCGAGCTGGCGCGCGAGGAGGTGACGGTGGCTTTGTCGGGCGACGGCGCCGACGAGGCGTTTGCGGGCT
ATCGGCGGCTGGTGTTCCAGCATCAGGAAGAAAAGCTGCGCGGGCTGATCCCCGGTTTCCTGCGCCGCGGTGTGCTGGGC
CCGCTATCGCATGTCTGGCCGCAGATGGACTGGGCGCCGCGTCCGCTCCGCGCGCGTGCAACGCTCGCCAGCCTGTCGAA
GAGCGGCGCGGAGGGTTATGCCGAGGCGGTCGGGGTGACGGGACCGGTGCAGCGCGCGCGGCTGTTCAACGATGCAGCGA
TCCGCGGGCTGGGCGATCATGTCGCCGAGGGGCGATACTGGAAGGCGATGGCGGAGGCGCCCGCGCGCGAACCGCTCGAC
CGCGCGCAATATGCCGACCTCAAAATCTGGCTGCCCGGCGACATTTTGACCAAGACCGACCGGATGAGCATGGCGGTCAG
CCTGGAGGCGCGCGAGCCTTTGCTCGATTACCGGCTCGTCGAGTTCGCCGCGAGCCTGCCCGCGTCGATGCGGGTCAAGG
GCGGCACGGGCAAGGCGCTATTGAAGCAGGCGATGGAACGCTATCTGCCGCACGACATCCTCTATCGGCCCAAGATGGGG
TTCGTGACGCCAGTGTCGGCCTGGTTCCGCGGCGCGCTCGCCCAGCAGGCGAAGGCGCTGGCAACCTCGTCTACGCTTGC
CCGGAGCGGCTGGTTCGACATGGCCGAGATCGAGCGGATCGTCGCCGCGCACCAGTCGGGCCGCCGCGACCATGGGCGGT
TGATCTGGCAATTTTTCATGCTGGAAAAGTCGCTGGCGAAGCTGTTCGGGATCTGA

Upstream 100 bases:

>100_bases
GGCGCAGGGCGGGCGCGATGCGATCAGGGATTTCGTTTCCGCGTCGGGCGGAGCCAGGGCGATGGCTGACCGCGCGCTCA
AAAGCCGCTAGGACCGACCC

Downstream 100 bases:

>100_bases
TCCCGGTGCGTCCGCTTGGAAGGTCGGGGCCGTCAGTCGTTTTGGCCAAAGGTCAGTAAAGGTCAGCCTTGTGCGTCAAG
CAGCTTGCCGCGCCTGCCGG

Product: asparagine synthase, glutamine-hydrolyzing

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 631; Mature: 631

Protein sequence:

>631_residues
MCGIAGIYHLETAKPVDPARLRAMLQPMQHRGPDGSGEWTAPGVGLAHLRLSIIDIAGSPQPMASDDEAVTLTYNGEIYN
FRELRAELEDRGHRFRTSGDTEVIIAAWRQWGPECLSRLNGMFAFAIHDHARGCLFLARDRLGVKPLHYARLADGSVAFA
SELKGLLRHPLLRQEASFSAVEDFFALGYVPDDNCIVAGIEKLPAGHYLMLERGKGVPAPTCWWAPDFSRRIKASEGEAA
EHLVYLMRAAVTDRMVADVPLGAFLSGGVDSSAVVALMAEASAKAVKTCTIGFDQAALDETAYAQQIAERFATDHRTRTV
AAGDFALIDRIADMFDEPFADASALPTYRVCELAREEVTVALSGDGADEAFAGYRRLVFQHQEEKLRGLIPGFLRRGVLG
PLSHVWPQMDWAPRPLRARATLASLSKSGAEGYAEAVGVTGPVQRARLFNDAAIRGLGDHVAEGRYWKAMAEAPAREPLD
RAQYADLKIWLPGDILTKTDRMSMAVSLEAREPLLDYRLVEFAASLPASMRVKGGTGKALLKQAMERYLPHDILYRPKMG
FVTPVSAWFRGALAQQAKALATSSTLARSGWFDMAEIERIVAAHQSGRRDHGRLIWQFFMLEKSLAKLFGI

Sequences:

>Translated_631_residues
MCGIAGIYHLETAKPVDPARLRAMLQPMQHRGPDGSGEWTAPGVGLAHLRLSIIDIAGSPQPMASDDEAVTLTYNGEIYN
FRELRAELEDRGHRFRTSGDTEVIIAAWRQWGPECLSRLNGMFAFAIHDHARGCLFLARDRLGVKPLHYARLADGSVAFA
SELKGLLRHPLLRQEASFSAVEDFFALGYVPDDNCIVAGIEKLPAGHYLMLERGKGVPAPTCWWAPDFSRRIKASEGEAA
EHLVYLMRAAVTDRMVADVPLGAFLSGGVDSSAVVALMAEASAKAVKTCTIGFDQAALDETAYAQQIAERFATDHRTRTV
AAGDFALIDRIADMFDEPFADASALPTYRVCELAREEVTVALSGDGADEAFAGYRRLVFQHQEEKLRGLIPGFLRRGVLG
PLSHVWPQMDWAPRPLRARATLASLSKSGAEGYAEAVGVTGPVQRARLFNDAAIRGLGDHVAEGRYWKAMAEAPAREPLD
RAQYADLKIWLPGDILTKTDRMSMAVSLEAREPLLDYRLVEFAASLPASMRVKGGTGKALLKQAMERYLPHDILYRPKMG
FVTPVSAWFRGALAQQAKALATSSTLARSGWFDMAEIERIVAAHQSGRRDHGRLIWQFFMLEKSLAKLFGI
>Mature_631_residues
MCGIAGIYHLETAKPVDPARLRAMLQPMQHRGPDGSGEWTAPGVGLAHLRLSIIDIAGSPQPMASDDEAVTLTYNGEIYN
FRELRAELEDRGHRFRTSGDTEVIIAAWRQWGPECLSRLNGMFAFAIHDHARGCLFLARDRLGVKPLHYARLADGSVAFA
SELKGLLRHPLLRQEASFSAVEDFFALGYVPDDNCIVAGIEKLPAGHYLMLERGKGVPAPTCWWAPDFSRRIKASEGEAA
EHLVYLMRAAVTDRMVADVPLGAFLSGGVDSSAVVALMAEASAKAVKTCTIGFDQAALDETAYAQQIAERFATDHRTRTV
AAGDFALIDRIADMFDEPFADASALPTYRVCELAREEVTVALSGDGADEAFAGYRRLVFQHQEEKLRGLIPGFLRRGVLG
PLSHVWPQMDWAPRPLRARATLASLSKSGAEGYAEAVGVTGPVQRARLFNDAAIRGLGDHVAEGRYWKAMAEAPAREPLD
RAQYADLKIWLPGDILTKTDRMSMAVSLEAREPLLDYRLVEFAASLPASMRVKGGTGKALLKQAMERYLPHDILYRPKMG
FVTPVSAWFRGALAQQAKALATSSTLARSGWFDMAEIERIVAAHQSGRRDHGRLIWQFFMLEKSLAKLFGI

Specific function: Main asparagine synthetase in vegetative cells [H]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI168229248, Length=397, Percent_Identity=29.2191435768262, Blast_Score=107, Evalue=5e-23,
Organism=Homo sapiens, GI168229252, Length=397, Percent_Identity=29.2191435768262, Blast_Score=107, Evalue=5e-23,
Organism=Homo sapiens, GI168229250, Length=397, Percent_Identity=29.2191435768262, Blast_Score=107, Evalue=5e-23,
Organism=Homo sapiens, GI296010848, Length=368, Percent_Identity=29.3478260869565, Blast_Score=104, Evalue=3e-22,
Organism=Homo sapiens, GI296010852, Length=297, Percent_Identity=29.2929292929293, Blast_Score=83, Evalue=9e-16,
Organism=Homo sapiens, GI296010850, Length=297, Percent_Identity=29.2929292929293, Blast_Score=83, Evalue=9e-16,
Organism=Escherichia coli, GI1786889, Length=420, Percent_Identity=30.952380952381, Blast_Score=159, Evalue=5e-40,
Organism=Caenorhabditis elegans, GI71993933, Length=388, Percent_Identity=31.4432989690722, Blast_Score=135, Evalue=7e-32,
Organism=Caenorhabditis elegans, GI25147557, Length=388, Percent_Identity=31.701030927835, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI25147560, Length=354, Percent_Identity=30.5084745762712, Blast_Score=122, Evalue=4e-28,
Organism=Saccharomyces cerevisiae, GI6321563, Length=411, Percent_Identity=28.7104622871046, Blast_Score=122, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6325403, Length=413, Percent_Identity=28.3292978208232, Blast_Score=118, Evalue=4e-27,
Organism=Drosophila melanogaster, GI45553209, Length=403, Percent_Identity=29.2803970223325, Blast_Score=130, Evalue=2e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 69332; Mature: 69332

Theoretical pI: Translated: 7.12; Mature: 7.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGIAGIYHLETAKPVDPARLRAMLQPMQHRGPDGSGEWTAPGVGLAHLRLSIIDIAGSP
CCCCCCEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHEEEEEEECCCC
QPMASDDEAVTLTYNGEIYNFRELRAELEDRGHRFRTSGDTEVIIAAWRQWGPECLSRLN
CCCCCCCCEEEEEECCCEEHHHHHHHHHHHCCCEECCCCCCEEEEHHHHHCCHHHHHHHC
GMFAFAIHDHARGCLFLARDRLGVKPLHYARLADGSVAFASELKGLLRHPLLRQEASFSA
CEEEEEEECCCCCEEEEECCCCCCCCCHHHHHCCCCHHHHHHHHHHHHCHHHHHHCCHHH
VEDFFALGYVPDDNCIVAGIEKLPAGHYLMLERGKGVPAPTCWWAPDFSRRIKASEGEAA
HHHHHHHCCCCCCCEEEECHHHCCCCCEEEEECCCCCCCCCCCCCCCHHHHCCCCCCHHH
EHLVYLMRAAVTDRMVADVPLGAFLSGGVDSSAVVALMAEASAKAVKTCTIGFDQAALDE
HHHHHHHHHHHHHHHHHHCCHHHHHCCCCCHHHHHHHHHHHHHHHHEEEECCCCHHHHHH
TAYAQQIAERFATDHRTRTVAAGDFALIDRIADMFDEPFADASALPTYRVCELAREEVTV
HHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHEEE
ALSGDGADEAFAGYRRLVFQHQEEKLRGLIPGFLRRGVLGPLSHVWPQMDWAPRPLRARA
EEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCHHHHH
TLASLSKSGAEGYAEAVGVTGPVQRARLFNDAAIRGLGDHVAEGRYWKAMAEAPAREPLD
HHHHHHHCCCCHHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHCCHHHHHHHHCCCCCCHH
RAQYADLKIWLPGDILTKTDRMSMAVSLEAREPLLDYRLVEFAASLPASMRVKGGTGKAL
HHCCCCEEEEECCCHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCEEECCCCCHHH
LKQAMERYLPHDILYRPKMGFVTPVSAWFRGALAQQAKALATSSTLARSGWFDMAEIERI
HHHHHHHHCCHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
VAAHQSGRRDHGRLIWQFFMLEKSLAKLFGI
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MCGIAGIYHLETAKPVDPARLRAMLQPMQHRGPDGSGEWTAPGVGLAHLRLSIIDIAGSP
CCCCCCEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHEEEEEEECCCC
QPMASDDEAVTLTYNGEIYNFRELRAELEDRGHRFRTSGDTEVIIAAWRQWGPECLSRLN
CCCCCCCCEEEEEECCCEEHHHHHHHHHHHCCCEECCCCCCEEEEHHHHHCCHHHHHHHC
GMFAFAIHDHARGCLFLARDRLGVKPLHYARLADGSVAFASELKGLLRHPLLRQEASFSA
CEEEEEEECCCCCEEEEECCCCCCCCCHHHHHCCCCHHHHHHHHHHHHCHHHHHHCCHHH
VEDFFALGYVPDDNCIVAGIEKLPAGHYLMLERGKGVPAPTCWWAPDFSRRIKASEGEAA
HHHHHHHCCCCCCCEEEECHHHCCCCCEEEEECCCCCCCCCCCCCCCHHHHCCCCCCHHH
EHLVYLMRAAVTDRMVADVPLGAFLSGGVDSSAVVALMAEASAKAVKTCTIGFDQAALDE
HHHHHHHHHHHHHHHHHHCCHHHHHCCCCCHHHHHHHHHHHHHHHHEEEECCCCHHHHHH
TAYAQQIAERFATDHRTRTVAAGDFALIDRIADMFDEPFADASALPTYRVCELAREEVTV
HHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHEEE
ALSGDGADEAFAGYRRLVFQHQEEKLRGLIPGFLRRGVLGPLSHVWPQMDWAPRPLRARA
EEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCHHHHH
TLASLSKSGAEGYAEAVGVTGPVQRARLFNDAAIRGLGDHVAEGRYWKAMAEAPAREPLD
HHHHHHHCCCCHHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHCCHHHHHHHHCCCCCCHH
RAQYADLKIWLPGDILTKTDRMSMAVSLEAREPLLDYRLVEFAASLPASMRVKGGTGKAL
HHCCCCEEEEECCCHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCEEECCCCCHHH
LKQAMERYLPHDILYRPKMGFVTPVSAWFRGALAQQAKALATSSTLARSGWFDMAEIERI
HHHHHHHHCCHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
VAAHQSGRRDHGRLIWQFFMLEKSLAKLFGI
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 8755891; 10498721 [H]