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The map label for this gene is folE [H]

Identifier: 103487395

GI number: 103487395

Start: 2009778

End: 2010380

Strand: Reverse

Name: folE [H]

Synonym: Sala_1911

Alternate gene names: 103487395

Gene position: 2010380-2009778 (Counterclockwise)

Preceding gene: 103487397

Following gene: 103487389

Centisome position: 60.1

GC content: 64.18

Gene sequence:

>603_bases
ATGAGCGAATTGGGACAGGATTTGGGACCGGACGGCAAGCTGATCGTTCCGGATCATGTCGCGGACGCCGTGCGCACGCT
GATCGAATGGGCCGGCGACGATCCTGCGCGCGAAGGCCTGCTCGACACGCCACGCCGCGTCGCGCGCGCGTGGAAGGAAT
ATTGTTCGGGCTATGGCGAAGATCCTTCGATCCACCTCTCGCGCACCTTTGAGGAGGTTGGCGGCTATGACGAGGTCGTG
CTGCTGCGCGACATTCCCTTCCAGTCGCATTGCGAACATCATATGGCGCCGATCACGGGCAAGGCGTCGATCGCCTATCT
GCCCAGGGACCGCGTCGTCGGCATTTCCAAGCTCGCGCGCGTTCTCAATGGCTATGCGCGGCGGCTTCAGGTGCAGGAGC
GGCTGACCGCCGAAGTCGCGCAGTGCATCTGGGACAATCTCCAGCCGCACGGGGTCGCGGTGGTAATCGACGCGCAGCAT
GGCTGCATGACCGGACGCGGCGTCCGCACGCCCGGCGTCGGCATGGTAACGAGCCGCCTGCTCGGCTGTTTCCTCGAAGA
CCAGCGCAGCCGGAAGGAAGTGCTGGGTTTGATGGGCTATTGA

Upstream 100 bases:

>100_bases
CAACTTGATCGTCGACGACAAATTCTTCGTGTACGGGCCTTGCGTATCGACCGCCGCGCGCCGAAATGTGCGACATGATA
GCAACAGTGAAAGTGGGGCG

Downstream 100 bases:

>100_bases
TCCTCAGCGCCGCCACATTCTGAGCAACTGATACTGGACGGCTTGGAGTCGCGTGTAGTTTGCGGGCTCCATCTTGCCGC
CCTCCAGCGCCGCGATCTCG

Product: GTP cyclohydrolase I

Products: NA

Alternate protein names: GTP cyclohydrolase I; GTP-CH-I [H]

Number of amino acids: Translated: 200; Mature: 199

Protein sequence:

>200_residues
MSELGQDLGPDGKLIVPDHVADAVRTLIEWAGDDPAREGLLDTPRRVARAWKEYCSGYGEDPSIHLSRTFEEVGGYDEVV
LLRDIPFQSHCEHHMAPITGKASIAYLPRDRVVGISKLARVLNGYARRLQVQERLTAEVAQCIWDNLQPHGVAVVIDAQH
GCMTGRGVRTPGVGMVTSRLLGCFLEDQRSRKEVLGLMGY

Sequences:

>Translated_200_residues
MSELGQDLGPDGKLIVPDHVADAVRTLIEWAGDDPAREGLLDTPRRVARAWKEYCSGYGEDPSIHLSRTFEEVGGYDEVV
LLRDIPFQSHCEHHMAPITGKASIAYLPRDRVVGISKLARVLNGYARRLQVQERLTAEVAQCIWDNLQPHGVAVVIDAQH
GCMTGRGVRTPGVGMVTSRLLGCFLEDQRSRKEVLGLMGY
>Mature_199_residues
SELGQDLGPDGKLIVPDHVADAVRTLIEWAGDDPAREGLLDTPRRVARAWKEYCSGYGEDPSIHLSRTFEEVGGYDEVVL
LRDIPFQSHCEHHMAPITGKASIAYLPRDRVVGISKLARVLNGYARRLQVQERLTAEVAQCIWDNLQPHGVAVVIDAQHG
CMTGRGVRTPGVGMVTSRLLGCFLEDQRSRKEVLGLMGY

Specific function: Tetrahydrofolate biosynthesis; first step. [C]

COG id: COG0302

COG function: function code H; GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family [H]

Homologues:

Organism=Homo sapiens, GI66932968, Length=189, Percent_Identity=44.973544973545, Blast_Score=158, Evalue=3e-39,
Organism=Homo sapiens, GI4503949, Length=189, Percent_Identity=44.973544973545, Blast_Score=158, Evalue=3e-39,
Organism=Homo sapiens, GI66932970, Length=159, Percent_Identity=42.7672955974843, Blast_Score=125, Evalue=2e-29,
Organism=Homo sapiens, GI66932972, Length=149, Percent_Identity=44.9664429530201, Blast_Score=122, Evalue=1e-28,
Organism=Escherichia coli, GI1788476, Length=179, Percent_Identity=38.5474860335196, Blast_Score=122, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17560486, Length=193, Percent_Identity=41.4507772020725, Blast_Score=159, Evalue=8e-40,
Organism=Saccharomyces cerevisiae, GI6321706, Length=182, Percent_Identity=45.6043956043956, Blast_Score=161, Evalue=6e-41,
Organism=Drosophila melanogaster, GI24656782, Length=191, Percent_Identity=41.8848167539267, Blast_Score=144, Evalue=5e-35,
Organism=Drosophila melanogaster, GI24656796, Length=191, Percent_Identity=41.8848167539267, Blast_Score=143, Evalue=9e-35,
Organism=Drosophila melanogaster, GI24656791, Length=191, Percent_Identity=41.8848167539267, Blast_Score=142, Evalue=1e-34,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001474
- InterPro:   IPR020602
- InterPro:   IPR018234 [H]

Pfam domain/function: PF01227 GTP_cyclohydroI [H]

EC number: =3.5.4.16 [H]

Molecular weight: Translated: 22118; Mature: 21986

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: PS00859 GTP_CYCLOHYDROL_1_1 ; PS00860 GTP_CYCLOHYDROL_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSELGQDLGPDGKLIVPDHVADAVRTLIEWAGDDPAREGLLDTPRRVARAWKEYCSGYGE
CCCCCHHCCCCCCEECCHHHHHHHHHHHHHCCCCHHHCCCCCCHHHHHHHHHHHHCCCCC
DPSIHLSRTFEEVGGYDEVVLLRDIPFQSHCEHHMAPITGKASIAYLPRDRVVGISKLAR
CCCCHHHHHHHHHCCCCCEEEEECCCCHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHHH
VLNGYARRLQVQERLTAEVAQCIWDNLQPHGVAVVIDAQHGCMTGRGVRTPGVGMVTSRL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHH
LGCFLEDQRSRKEVLGLMGY
HHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SELGQDLGPDGKLIVPDHVADAVRTLIEWAGDDPAREGLLDTPRRVARAWKEYCSGYGE
CCCCHHCCCCCCEECCHHHHHHHHHHHHHCCCCHHHCCCCCCHHHHHHHHHHHHCCCCC
DPSIHLSRTFEEVGGYDEVVLLRDIPFQSHCEHHMAPITGKASIAYLPRDRVVGISKLAR
CCCCHHHHHHHHHCCCCCEEEEECCCCHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHHH
VLNGYARRLQVQERLTAEVAQCIWDNLQPHGVAVVIDAQHGCMTGRGVRTPGVGMVTSRL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHH
LGCFLEDQRSRKEVLGLMGY
HHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]