Click here to switch to the map view.

The map label for this gene is gcvH [H]

Identifier: 103487354

GI number: 103487354

Start: 1966243

End: 1966614

Strand: Reverse

Name: gcvH [H]

Synonym: Sala_1870

Alternate gene names: 103487354

Gene position: 1966614-1966243 (Counterclockwise)

Preceding gene: 103487355

Following gene: 103487353

Centisome position: 58.79

GC content: 62.37

Gene sequence:

>372_bases
ATGCCGCGTTATTATACCGAGGAACATGAGTGGATCGATGTCGATGGCGATGTCGCGACGGTCGGCATCACCGACTTTGC
GCAGGGCCAGCTTGGCGACATTGTGTTCGTCGAGGTTCCCGACACGGGTGCCGAGCTGAGCCAGGGCGGCGATGCCGCGG
TGGTCGAATCGGTGAAGGCGGCGAGCGACGTCTATGCCCCCGTCGATGGCACGGTGACCGAAGGCAATGCGCAACTCGAG
GAAGACCCCGCGCTCGTCAATTCGGATCCCGAAGGCGAGGGCTGGTTCTTTCGCATGACGCTGGCCGACAAGAGCCAGCT
CGACGCCCTGATGGACGCAAAGGCCTACAAGGCTTTCTGCGACGCCCTCTAA

Upstream 100 bases:

>100_bases
CGCGGTCGCCGCCGAGGTGCGCGGCAAGCGCGTCGCCTGCACCGTCACCGCCATGCCATTCATTCCGCACCGCTATGTGC
GCAAACAGGGAGCCTGACCG

Downstream 100 bases:

>100_bases
CCGACCGCCCCGCCCGCGGACGGGAGGGGGAAGGGACATGATGACCGCAGCCGATCCCGCGCTGGCCAAGTGCCGCCTGA
TGGTGGCGACGCCCATTTAT

Product: glycine cleavage system protein H

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 123; Mature: 122

Protein sequence:

>123_residues
MPRYYTEEHEWIDVDGDVATVGITDFAQGQLGDIVFVEVPDTGAELSQGGDAAVVESVKAASDVYAPVDGTVTEGNAQLE
EDPALVNSDPEGEGWFFRMTLADKSQLDALMDAKAYKAFCDAL

Sequences:

>Translated_123_residues
MPRYYTEEHEWIDVDGDVATVGITDFAQGQLGDIVFVEVPDTGAELSQGGDAAVVESVKAASDVYAPVDGTVTEGNAQLE
EDPALVNSDPEGEGWFFRMTLADKSQLDALMDAKAYKAFCDAL
>Mature_122_residues
PRYYTEEHEWIDVDGDVATVGITDFAQGQLGDIVFVEVPDTGAELSQGGDAAVVESVKAASDVYAPVDGTVTEGNAQLEE
DPALVNSDPEGEGWFFRMTLADKSQLDALMDAKAYKAFCDAL

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein [H]

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI49574537, Length=121, Percent_Identity=42.9752066115703, Blast_Score=120, Evalue=3e-28,
Organism=Homo sapiens, GI89057342, Length=121, Percent_Identity=42.9752066115703, Blast_Score=120, Evalue=3e-28,
Organism=Escherichia coli, GI1789271, Length=118, Percent_Identity=50.8474576271186, Blast_Score=122, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI17507493, Length=117, Percent_Identity=49.5726495726496, Blast_Score=134, Evalue=8e-33,
Organism=Caenorhabditis elegans, GI17551294, Length=117, Percent_Identity=48.7179487179487, Blast_Score=134, Evalue=9e-33,
Organism=Saccharomyces cerevisiae, GI6319272, Length=117, Percent_Identity=47.8632478632479, Blast_Score=113, Evalue=8e-27,
Organism=Drosophila melanogaster, GI17865652, Length=121, Percent_Identity=48.7603305785124, Blast_Score=123, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053 [H]

Pfam domain/function: PF01597 GCV_H [H]

EC number: NA

Molecular weight: Translated: 13180; Mature: 13049

Theoretical pI: Translated: 3.59; Mature: 3.59

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRYYTEEHEWIDVDGDVATVGITDFAQGQLGDIVFVEVPDTGAELSQGGDAAVVESVKA
CCCCCCCCCCEEECCCCEEEEECCCCCCCCCCCEEEEEECCCCCHHCCCCCHHHHHHHHH
ASDVYAPVDGTVTEGNAQLEEDPALVNSDPEGEGWFFRMTLADKSQLDALMDAKAYKAFC
HCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHH
DAL
CCC
>Mature Secondary Structure 
PRYYTEEHEWIDVDGDVATVGITDFAQGQLGDIVFVEVPDTGAELSQGGDAAVVESVKA
CCCCCCCCCEEECCCCEEEEECCCCCCCCCCCEEEEEECCCCCHHCCCCCHHHHHHHHH
ASDVYAPVDGTVTEGNAQLEEDPALVNSDPEGEGWFFRMTLADKSQLDALMDAKAYKAFC
HCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHH
DAL
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11214968 [H]