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The map label for this gene is aspS [H]

Identifier: 103487236

GI number: 103487236

Start: 1843032

End: 1844891

Strand: Reverse

Name: aspS [H]

Synonym: Sala_1751

Alternate gene names: 103487236

Gene position: 1844891-1843032 (Counterclockwise)

Preceding gene: 103487238

Following gene: 103487235

Centisome position: 55.15

GC content: 64.46

Gene sequence:

>1860_bases
ATGGCTCGCCGACCTGCCTTAGGCGCGGCGATAAATCACTTTAAACGTGACATCCCCAAATCTTCGAAAGACCATGAAAT
GCACGCCTATCGCACCCATCATTGCGGCCAGCTTCGCGCCGAGCATGTCGGCCAGACTGTTCGCCTGTCGGGCTGGGTGC
ATCGCAAGCGCGATCATGGCGGGCTGCTCTTCGTCGATCTGCGCGACCATTATGGTCTGACGCAGATCGTCGTGGACTCG
AGCGATGCCGCTTTCGCGACGCTCGACGGCCTGCGCGCCGAAAGCGTCGTGACGATCACCGGCGACGTCGTCGCGCGTTC
GGCCGAGACGGTGAACGCGAACCTGCCGACCGGCGCGATCGAGGTGCGCGCGCGCGAGGTGGCGGTGCAGTCGGCGGCGG
CCGAGCTGCCGATGCCGGTCGCGGGCGAACAGGAGTATCCCGAGGACATCCGCCTCAAATATCGTTTCCTCGACCTGCGC
CGCGAGCGGCTGCACGCGAACATCATGCTGCGCTCGAACGTTATCGCGTCGCTCCGCCGCCGCATGGTCGAACAGGGCTT
TACCGAATATCAGACCCCGATCCTCACCGCCTCGTCGCCCGAAGGCGCGCGCGACTATCTGGTGCCCAGCCGCGTCCACC
CCGGCAAATTCTATGCGCTGCCGCAGGCACCGCAGATGTTCAAGCAGCTTCTGATGGTCGCGGGCTTCGACCGCTATTTC
CAGATCGCGCCCTGCTTCCGCGACGAGGATGCGCGAGCCGACCGTAGCCCGGGCGAATTTTATCAGCTCGATTTCGAAAT
GAGCTTCGTCACCCAGGACGACGTCTTCAACGCGATCGAACCCGTGCTCGCGGGCGTGTTCGAAGAGTTTGCGAACGGCA
AGTCGGTGACGCCCGCCGGCTCCTTTCCGCGCATCCCGTACCGCGAGTCGATGCTGAAGTACGGCAACGACAAGCCCGAC
CTGCGCAACCCGCTGATCATCAGCGACGTGTCGGCGCATTTCACCGGATCGGGCTTCGGCCGCTTCGCCGACATCGTCGC
GGCGGGCGATGTCGTGCGCGCGATTCCCGCGCCCGCGACGGCCGACAAGAGCCGCAAGTTCTTCGACGACATGAACAGCT
GGGCGCAGGGCGAAGGCTTTGCCGGCCTCGGCTATGCGACGCGCAAGGGCGGCGAATGGGGCGGCCCGATCGCCAAGAAC
CACGGGGCGGAGAAAATGGACGCGCTCGCCGCCGACCTCGGCCTCGGCCCCGACGATGGGTTGTTCTTCGCCGCGGGCAG
GGAAGCGCAGGCGGCGAAGCTCGCGGGCCTCGCGCGCACCCGCGTCGCCGAGCAACTGGGCCTCATCGACCCGAACAGGT
TCGAAATGTGCTGGATCGTCGATTTCCCGATGTTCGAGCGCGACGAGGAAACGGGCAAGATCGACTTCAGCCACAATCCC
TTCTCGATGCCGCAGGGTGAGCTTGAGGCGCTCGAGACCAAAGACCCGCTCGACATTCTCGCCTGGCAATATGACATCGT
CTGCAACGGCGTCGAACTGTCGTCGGGCGCGATCCGCAACCATCGCCCCGACATCATGTACAAGGCGTTCGAAATTGCGG
GCTATAGCCAGGCCGAGGTCGACGCCAATTTCGCGGGCATGATCAACGCGTTCAAATATGGCGCGCCGCCGCATGGCGGG
TCGGCGCCGGGGGTCGACCGCATCGTCATGCTGCTCGCCGACGAGCCCAATATCCGCGAAGTCGTCGTCTTCCCGATGAA
CCAGAAGGCCGAGGACCTGATGATGGGTGCCCCGGCGCCGGTATCAGAGAAGCAGCTCAGGGAACTCGGAATCCGCATTG
TCGACGGTCCGAAGAGCTAA

Upstream 100 bases:

>100_bases
GAGCAACGCGTCGCCCCGCAAAGGCGCGGTCGGCGGCAATCTTGCGCAGGGCCGATGGCGGCCCGCGCCGTCGCGTGGAC
GACGAGGCAAGCCTTGACAA

Downstream 100 bases:

>100_bases
AGCGTGAGCGAACCCCGGGAGCATCGGCTGGAAACGCCGGACGGTGCGATCTGCTGGTTCGAATGGGGTGAGCGCGGCGA
GGCCGCTTCGCTGCTCCTGC

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS [H]

Number of amino acids: Translated: 619; Mature: 618

Protein sequence:

>619_residues
MARRPALGAAINHFKRDIPKSSKDHEMHAYRTHHCGQLRAEHVGQTVRLSGWVHRKRDHGGLLFVDLRDHYGLTQIVVDS
SDAAFATLDGLRAESVVTITGDVVARSAETVNANLPTGAIEVRAREVAVQSAAAELPMPVAGEQEYPEDIRLKYRFLDLR
RERLHANIMLRSNVIASLRRRMVEQGFTEYQTPILTASSPEGARDYLVPSRVHPGKFYALPQAPQMFKQLLMVAGFDRYF
QIAPCFRDEDARADRSPGEFYQLDFEMSFVTQDDVFNAIEPVLAGVFEEFANGKSVTPAGSFPRIPYRESMLKYGNDKPD
LRNPLIISDVSAHFTGSGFGRFADIVAAGDVVRAIPAPATADKSRKFFDDMNSWAQGEGFAGLGYATRKGGEWGGPIAKN
HGAEKMDALAADLGLGPDDGLFFAAGREAQAAKLAGLARTRVAEQLGLIDPNRFEMCWIVDFPMFERDEETGKIDFSHNP
FSMPQGELEALETKDPLDILAWQYDIVCNGVELSSGAIRNHRPDIMYKAFEIAGYSQAEVDANFAGMINAFKYGAPPHGG
SAPGVDRIVMLLADEPNIREVVVFPMNQKAEDLMMGAPAPVSEKQLRELGIRIVDGPKS

Sequences:

>Translated_619_residues
MARRPALGAAINHFKRDIPKSSKDHEMHAYRTHHCGQLRAEHVGQTVRLSGWVHRKRDHGGLLFVDLRDHYGLTQIVVDS
SDAAFATLDGLRAESVVTITGDVVARSAETVNANLPTGAIEVRAREVAVQSAAAELPMPVAGEQEYPEDIRLKYRFLDLR
RERLHANIMLRSNVIASLRRRMVEQGFTEYQTPILTASSPEGARDYLVPSRVHPGKFYALPQAPQMFKQLLMVAGFDRYF
QIAPCFRDEDARADRSPGEFYQLDFEMSFVTQDDVFNAIEPVLAGVFEEFANGKSVTPAGSFPRIPYRESMLKYGNDKPD
LRNPLIISDVSAHFTGSGFGRFADIVAAGDVVRAIPAPATADKSRKFFDDMNSWAQGEGFAGLGYATRKGGEWGGPIAKN
HGAEKMDALAADLGLGPDDGLFFAAGREAQAAKLAGLARTRVAEQLGLIDPNRFEMCWIVDFPMFERDEETGKIDFSHNP
FSMPQGELEALETKDPLDILAWQYDIVCNGVELSSGAIRNHRPDIMYKAFEIAGYSQAEVDANFAGMINAFKYGAPPHGG
SAPGVDRIVMLLADEPNIREVVVFPMNQKAEDLMMGAPAPVSEKQLRELGIRIVDGPKS
>Mature_618_residues
ARRPALGAAINHFKRDIPKSSKDHEMHAYRTHHCGQLRAEHVGQTVRLSGWVHRKRDHGGLLFVDLRDHYGLTQIVVDSS
DAAFATLDGLRAESVVTITGDVVARSAETVNANLPTGAIEVRAREVAVQSAAAELPMPVAGEQEYPEDIRLKYRFLDLRR
ERLHANIMLRSNVIASLRRRMVEQGFTEYQTPILTASSPEGARDYLVPSRVHPGKFYALPQAPQMFKQLLMVAGFDRYFQ
IAPCFRDEDARADRSPGEFYQLDFEMSFVTQDDVFNAIEPVLAGVFEEFANGKSVTPAGSFPRIPYRESMLKYGNDKPDL
RNPLIISDVSAHFTGSGFGRFADIVAAGDVVRAIPAPATADKSRKFFDDMNSWAQGEGFAGLGYATRKGGEWGGPIAKNH
GAEKMDALAADLGLGPDDGLFFAAGREAQAAKLAGLARTRVAEQLGLIDPNRFEMCWIVDFPMFERDEETGKIDFSHNPF
SMPQGELEALETKDPLDILAWQYDIVCNGVELSSGAIRNHRPDIMYKAFEIAGYSQAEVDANFAGMINAFKYGAPPHGGS
APGVDRIVMLLADEPNIREVVVFPMNQKAEDLMMGAPAPVSEKQLRELGIRIVDGPKS

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI40789249, Length=617, Percent_Identity=35.3322528363047, Blast_Score=350, Evalue=3e-96,
Organism=Homo sapiens, GI45439306, Length=293, Percent_Identity=25.938566552901, Blast_Score=93, Evalue=6e-19,
Organism=Homo sapiens, GI226491502, Length=253, Percent_Identity=25.6916996047431, Blast_Score=81, Evalue=3e-15,
Organism=Homo sapiens, GI4758762, Length=298, Percent_Identity=27.1812080536913, Blast_Score=76, Evalue=1e-13,
Organism=Homo sapiens, GI5031815, Length=262, Percent_Identity=24.8091603053435, Blast_Score=74, Evalue=6e-13,
Organism=Homo sapiens, GI194272210, Length=262, Percent_Identity=24.8091603053435, Blast_Score=73, Evalue=7e-13,
Organism=Escherichia coli, GI1788173, Length=593, Percent_Identity=40.9780775716695, Blast_Score=428, Evalue=1e-121,
Organism=Escherichia coli, GI1790571, Length=326, Percent_Identity=25.4601226993865, Blast_Score=78, Evalue=2e-15,
Organism=Escherichia coli, GI1789256, Length=342, Percent_Identity=25.7309941520468, Blast_Score=76, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI32566633, Length=606, Percent_Identity=34.983498349835, Blast_Score=311, Evalue=9e-85,
Organism=Caenorhabditis elegans, GI71984122, Length=303, Percent_Identity=25.7425742574257, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17551876, Length=276, Percent_Identity=24.2753623188406, Blast_Score=81, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI71994340, Length=251, Percent_Identity=26.2948207171315, Blast_Score=80, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17535927, Length=251, Percent_Identity=26.2948207171315, Blast_Score=79, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17535925, Length=251, Percent_Identity=26.2948207171315, Blast_Score=79, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6325153, Length=353, Percent_Identity=33.7110481586402, Blast_Score=172, Evalue=1e-43,
Organism=Saccharomyces cerevisiae, GI6321807, Length=291, Percent_Identity=28.5223367697594, Blast_Score=92, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6323011, Length=270, Percent_Identity=28.5185185185185, Blast_Score=84, Evalue=7e-17,
Organism=Drosophila melanogaster, GI24584738, Length=606, Percent_Identity=34.8184818481848, Blast_Score=331, Evalue=9e-91,
Organism=Drosophila melanogaster, GI17136276, Length=262, Percent_Identity=27.8625954198473, Blast_Score=93, Evalue=6e-19,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.12 [H]

Molecular weight: Translated: 68275; Mature: 68144

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARRPALGAAINHFKRDIPKSSKDHEMHAYRTHHCGQLRAEHVGQTVRLSGWVHRKRDHG
CCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHCCHHHHHHHCCCEEEECCEEEEECCCC
GLLFVDLRDHYGLTQIVVDSSDAAFATLDGLRAESVVTITGDVVARSAETVNANLPTGAI
CEEEEEECCCCCEEEEEEECCCCCEEECCCCCCCEEEEEEHHHHHCCCCEEECCCCCCEE
EVRAREVAVQSAAAELPMPVAGEQEYPEDIRLKYRFLDLRRERLHANIMLRSNVIASLRR
EEHHHHHHHHHHHHHCCCCCCCCCCCCHHHEEEHHHHHHHHHHHHHEEEEHHHHHHHHHH
RMVEQGFTEYQTPILTASSPEGARDYLVPSRVHPGKFYALPQAPQMFKQLLMVAGFDRYF
HHHHCCCHHHCCCEEECCCCCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHCCCHHE
QIAPCFRDEDARADRSPGEFYQLDFEMSFVTQDDVFNAIEPVLAGVFEEFANGKSVTPAG
EECCCCCCCCCCCCCCCCCEEEEEEEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
SFPRIPYRESMLKYGNDKPDLRNPLIISDVSAHFTGSGFGRFADIVAAGDVVRAIPAPAT
CCCCCCHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHCHHHHHCCCCCC
ADKSRKFFDDMNSWAQGEGFAGLGYATRKGGEWGGPIAKNHGAEKMDALAADLGLGPDDG
CCHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCC
LFFAAGREAQAAKLAGLARTRVAEQLGLIDPNRFEMCWIVDFPMFERDEETGKIDFSHNP
EEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCCCEEEECCCC
FSMPQGELEALETKDPLDILAWQYDIVCNGVELSSGAIRNHRPDIMYKAFEIAGYSQAEV
CCCCCCHHHHHCCCCCHHHEEEEEEEEECCEEECCCCCCCCCCCCEEEEHHHCCCCHHHC
DANFAGMINAFKYGAPPHGGSAPGVDRIVMLLADEPNIREVVVFPMNQKAEDLMMGAPAP
CCCHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCEEEEEECCCCCHHHHHCCCCCC
VSEKQLRELGIRIVDGPKS
CCHHHHHHCCCEEECCCCC
>Mature Secondary Structure 
ARRPALGAAINHFKRDIPKSSKDHEMHAYRTHHCGQLRAEHVGQTVRLSGWVHRKRDHG
CCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHCCHHHHHHHCCCEEEECCEEEEECCCC
GLLFVDLRDHYGLTQIVVDSSDAAFATLDGLRAESVVTITGDVVARSAETVNANLPTGAI
CEEEEEECCCCCEEEEEEECCCCCEEECCCCCCCEEEEEEHHHHHCCCCEEECCCCCCEE
EVRAREVAVQSAAAELPMPVAGEQEYPEDIRLKYRFLDLRRERLHANIMLRSNVIASLRR
EEHHHHHHHHHHHHHCCCCCCCCCCCCHHHEEEHHHHHHHHHHHHHEEEEHHHHHHHHHH
RMVEQGFTEYQTPILTASSPEGARDYLVPSRVHPGKFYALPQAPQMFKQLLMVAGFDRYF
HHHHCCCHHHCCCEEECCCCCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHCCCHHE
QIAPCFRDEDARADRSPGEFYQLDFEMSFVTQDDVFNAIEPVLAGVFEEFANGKSVTPAG
EECCCCCCCCCCCCCCCCCEEEEEEEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
SFPRIPYRESMLKYGNDKPDLRNPLIISDVSAHFTGSGFGRFADIVAAGDVVRAIPAPAT
CCCCCCHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHCHHHHHCCCCCC
ADKSRKFFDDMNSWAQGEGFAGLGYATRKGGEWGGPIAKNHGAEKMDALAADLGLGPDDG
CCHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCC
LFFAAGREAQAAKLAGLARTRVAEQLGLIDPNRFEMCWIVDFPMFERDEETGKIDFSHNP
EEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCCCEEEECCCC
FSMPQGELEALETKDPLDILAWQYDIVCNGVELSSGAIRNHRPDIMYKAFEIAGYSQAEV
CCCCCCHHHHHCCCCCHHHEEEEEEEEECCEEECCCCCCCCCCCCEEEEHHHCCCCHHHC
DANFAGMINAFKYGAPPHGGSAPGVDRIVMLLADEPNIREVVVFPMNQKAEDLMMGAPAP
CCCHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCEEEEEECCCCCHHHHHCCCCCC
VSEKQLRELGIRIVDGPKS
CCHHHHHHCCCEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA