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The map label for this gene is amiD [H]

Identifier: 103487220

GI number: 103487220

Start: 1828067

End: 1828756

Strand: Reverse

Name: amiD [H]

Synonym: Sala_1735

Alternate gene names: 103487220

Gene position: 1828756-1828067 (Counterclockwise)

Preceding gene: 103487221

Following gene: 103487219

Centisome position: 54.67

GC content: 64.06

Gene sequence:

>690_bases
ATGAGCGATTTTGTCGAACGCTGGTCCCCCAATTTCGACGAGCGCGCGCTGCCCGTTTCGATGATCGTCCTGCATTATAC
CGGCATGAAGACCGGCGCCGAAGCCATTGATCGGCTTGCGGATCCCGACGCCAGGGTTTCGGCCCATTATGTCGTCAGCG
AGGACGGTCAGATCACCCATATGGTGCCCGAGGACAAGCGCGCCTGGCACGCGGGCAAGTCGCACTGGCGCGGCATCAGC
GACATCAATTCGGCGAGCATCGGGATCGAGATCGTCAATCCGGGGCACGAATATGGCTATGTGCCCTTTCCCGAACCGCA
GGTGGCGTCGGTCGTGCGGCTCGTCCACCTGATCAAGGACCGCTATGCGATCACGCGTGGCAATGTCGTCGGCCATTCGG
ACATCGCCCCGACGCGCAAGCAGGATCCGGGCGAGCTGTTTCCGTGGGAGGAGCTTGCTCGCCGCCGCCTCGCGCTGCCG
CGCCCGACCAAGAAGCTCACCGATCCCCTGTGGACCGACGCGGGTTTCCTGCTCGCGCTCGAACGCTTTGGCTATGATGT
GACCGACGGATTTGCGGCGACGGTCGCGTTCCAGCGGCGCTTTCGTCCCGAGCTGATCGACGGGACGATCGACGGCGAAT
GCCGGGCGATCCTGCTGGCGCTGCTGCTGCCGCAGCCCGAGGGAAATTGA

Upstream 100 bases:

>100_bases
CGCCCCGACACCGCGCGGTCGATCGCCGCCTGACCTTGCTTTTTGTCCCCCGCTGGCGCAAGCGACGGTCAGGCAGCCAC
AGCACGGGCCCAATAAGCGC

Downstream 100 bases:

>100_bases
GGGGCGGCTGACGACCGATTGCGGCCGCCGCGCTCTTCCCCCTGCGGGGGAGGAACGTCCGCTCCCCACCCAAAGCCGCC
ATCCTCCACATTCGCTCGCC

Product: N-acetylmuramyl-L-alanine amidase, negative regulator of AmpC, AmpD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 229; Mature: 228

Protein sequence:

>229_residues
MSDFVERWSPNFDERALPVSMIVLHYTGMKTGAEAIDRLADPDARVSAHYVVSEDGQITHMVPEDKRAWHAGKSHWRGIS
DINSASIGIEIVNPGHEYGYVPFPEPQVASVVRLVHLIKDRYAITRGNVVGHSDIAPTRKQDPGELFPWEELARRRLALP
RPTKKLTDPLWTDAGFLLALERFGYDVTDGFAATVAFQRRFRPELIDGTIDGECRAILLALLLPQPEGN

Sequences:

>Translated_229_residues
MSDFVERWSPNFDERALPVSMIVLHYTGMKTGAEAIDRLADPDARVSAHYVVSEDGQITHMVPEDKRAWHAGKSHWRGIS
DINSASIGIEIVNPGHEYGYVPFPEPQVASVVRLVHLIKDRYAITRGNVVGHSDIAPTRKQDPGELFPWEELARRRLALP
RPTKKLTDPLWTDAGFLLALERFGYDVTDGFAATVAFQRRFRPELIDGTIDGECRAILLALLLPQPEGN
>Mature_228_residues
SDFVERWSPNFDERALPVSMIVLHYTGMKTGAEAIDRLADPDARVSAHYVVSEDGQITHMVPEDKRAWHAGKSHWRGISD
INSASIGIEIVNPGHEYGYVPFPEPQVASVVRLVHLIKDRYAITRGNVVGHSDIAPTRKQDPGELFPWEELARRRLALPR
PTKKLTDPLWTDAGFLLALERFGYDVTDGFAATVAFQRRFRPELIDGTIDGECRAILLALLLPQPEGN

Specific function: Unknown

COG id: COG3023

COG function: function code V; Negative regulator of beta-lactamase expression

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1787092, Length=233, Percent_Identity=37.7682403433476, Blast_Score=124, Evalue=6e-30,
Organism=Escherichia coli, GI1786300, Length=119, Percent_Identity=42.8571428571429, Blast_Score=84, Evalue=9e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502
- InterPro:   IPR002477 [H]

Pfam domain/function: PF01510 Amidase_2 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 25663; Mature: 25532

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDFVERWSPNFDERALPVSMIVLHYTGMKTGAEAIDRLADPDARVSAHYVVSEDGQITH
CCCHHHHHCCCCCCCCCHHHEEEEHHCCCCHHHHHHHHHCCCCCCEEEEEEECCCCCEEE
MVPEDKRAWHAGKSHWRGISDINSASIGIEIVNPGHEYGYVPFPEPQVASVVRLVHLIKD
ECCCCHHHHHCCHHHCCCCCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHH
RYAITRGNVVGHSDIAPTRKQDPGELFPWEELARRRLALPRPTKKLTDPLWTDAGFLLAL
HHHEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCHHHHCCCHHCCCHHHHHH
ERFGYDVTDGFAATVAFQRRFRPELIDGTIDGECRAILLALLLPQPEGN
HHHCCCCCCHHHHHHHHHHHCCHHHHCCCCCHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SDFVERWSPNFDERALPVSMIVLHYTGMKTGAEAIDRLADPDARVSAHYVVSEDGQITH
CCHHHHHCCCCCCCCCHHHEEEEHHCCCCHHHHHHHHHCCCCCCEEEEEEECCCCCEEE
MVPEDKRAWHAGKSHWRGISDINSASIGIEIVNPGHEYGYVPFPEPQVASVVRLVHLIKD
ECCCCHHHHHCCHHHCCCCCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHH
RYAITRGNVVGHSDIAPTRKQDPGELFPWEELARRRLALPRPTKKLTDPLWTDAGFLLAL
HHHEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCHHHHCCCHHCCCHHHHHH
ERFGYDVTDGFAATVAFQRRFRPELIDGTIDGECRAILLALLLPQPEGN
HHHCCCCCCHHHHHHHHHHHCCHHHHCCCCCHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]